Crimson clover cryptic virus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; Betapartitivirus

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|M9VTV7|M9VTV7_9VIRU RNA-dependent RNA polymerase OS=Crimson clover cryptic virus 2 OX=1323528 PE=4 SV=1
MM1 pKa = 6.65TTNSSNARR9 pKa = 11.84LNKK12 pKa = 10.01LKK14 pKa = 10.96NNTVPLPFNATEE26 pKa = 4.25VPEE29 pKa = 4.37PATDD33 pKa = 4.05LLTSMKK39 pKa = 9.92EE40 pKa = 3.71NTSAHH45 pKa = 6.22SATANQWTIDD55 pKa = 4.26LFPNMIPVLIHH66 pKa = 5.69VMSAASHH73 pKa = 6.21HH74 pKa = 6.58ANAINFRR81 pKa = 11.84EE82 pKa = 3.99HH83 pKa = 6.8SKK85 pKa = 9.98TSAATVAMYY94 pKa = 10.8HH95 pKa = 5.44MAVVYY100 pKa = 9.69GYY102 pKa = 11.15FLLNDD107 pKa = 3.75LNIRR111 pKa = 11.84LNPSAHH117 pKa = 5.41ARR119 pKa = 11.84SWAEE123 pKa = 3.72ASWKK127 pKa = 10.63SEE129 pKa = 3.62FVEE132 pKa = 4.31FLYY135 pKa = 10.85GLPVPEE141 pKa = 4.81FLAPILSEE149 pKa = 4.01YY150 pKa = 9.56QHH152 pKa = 6.52FVTDD156 pKa = 3.54KK157 pKa = 9.32TPNVHH162 pKa = 6.1FTPSAAGFDD171 pKa = 3.42HH172 pKa = 7.29DD173 pKa = 4.07QFFGRR178 pKa = 11.84VFPLNMFAALHH189 pKa = 6.88DD190 pKa = 4.23STATLPSSSTYY201 pKa = 10.92AQVLQDD207 pKa = 3.98LFSRR211 pKa = 11.84PLYY214 pKa = 10.18TITDD218 pKa = 3.97PAFTCYY224 pKa = 10.54LPDD227 pKa = 3.95LLGITLTAGEE237 pKa = 4.52NVTTLNYY244 pKa = 9.87INSKK248 pKa = 9.92LYY250 pKa = 10.61QVFTTIFNPVLFRR263 pKa = 11.84DD264 pKa = 3.71HH265 pKa = 5.86QRR267 pKa = 11.84RR268 pKa = 11.84SSLAALSFKK277 pKa = 11.0SPTYY281 pKa = 10.11PNPNINAYY289 pKa = 10.03DD290 pKa = 4.04LLFSASVANLRR301 pKa = 11.84EE302 pKa = 4.15IKK304 pKa = 10.2IVLQSIYY311 pKa = 10.83KK312 pKa = 10.44LFDD315 pKa = 3.01GRR317 pKa = 11.84VACKK321 pKa = 8.81HH322 pKa = 5.5TLSQFICEE330 pKa = 4.3STSPSITKK338 pKa = 9.82HH339 pKa = 5.7GYY341 pKa = 7.84STFPLPTWSHH351 pKa = 5.78TEE353 pKa = 3.61SDD355 pKa = 3.07AKK357 pKa = 10.62AIRR360 pKa = 11.84FSGVTALVHH369 pKa = 6.22VSEE372 pKa = 4.87EE373 pKa = 3.76DD374 pKa = 3.33RR375 pKa = 11.84AQDD378 pKa = 3.19FCFLQRR384 pKa = 11.84PAEE387 pKa = 4.67AIPHH391 pKa = 5.21VNEE394 pKa = 4.16VPDD397 pKa = 3.26IRR399 pKa = 11.84YY400 pKa = 7.49ATTEE404 pKa = 4.02DD405 pKa = 4.35PEE407 pKa = 4.63TAVPLPANHH416 pKa = 6.28ILVRR420 pKa = 11.84RR421 pKa = 11.84FPFAIRR427 pKa = 11.84LRR429 pKa = 11.84QEE431 pKa = 3.8AANGFPRR438 pKa = 11.84HH439 pKa = 6.74DD440 pKa = 4.16NDD442 pKa = 5.43DD443 pKa = 3.54LTKK446 pKa = 10.73FSDD449 pKa = 3.99HH450 pKa = 5.33VHH452 pKa = 5.51TAPRR456 pKa = 11.84VLILDD461 pKa = 3.7TTGDD465 pKa = 4.26GIISAHH471 pKa = 5.21TTTASGKK478 pKa = 9.58IIEE481 pKa = 4.39SFEE484 pKa = 4.67LDD486 pKa = 3.04GSTIEE491 pKa = 4.75MPNADD496 pKa = 4.58KK497 pKa = 11.17SLGMQNCLFADD508 pKa = 3.42SAIAYY513 pKa = 8.89KK514 pKa = 9.6YY515 pKa = 9.43VRR517 pKa = 11.84PGSFYY522 pKa = 10.78RR523 pKa = 11.84PRR525 pKa = 11.84TQGSVLPPLNRR536 pKa = 11.84APPNSRR542 pKa = 11.84PRR544 pKa = 11.84LPASSMLHH552 pKa = 6.68DD553 pKa = 3.68RR554 pKa = 11.84TKK556 pKa = 11.13VFLPQLNTRR565 pKa = 11.84INEE568 pKa = 4.06AVDD571 pKa = 3.04IRR573 pKa = 11.84TLPGLTMLNNVNCINYY589 pKa = 8.46AQSFIGFKK597 pKa = 10.19TVDD600 pKa = 2.95ATRR603 pKa = 11.84NDD605 pKa = 3.96DD606 pKa = 3.81VLDD609 pKa = 4.29AVPGMPLNNLILWSPYY625 pKa = 9.86TYY627 pKa = 9.79TAYY630 pKa = 10.19EE631 pKa = 4.44DD632 pKa = 3.49EE633 pKa = 4.85TYY635 pKa = 10.56PDD637 pKa = 3.83PVFSNSRR644 pKa = 11.84HH645 pKa = 5.29YY646 pKa = 11.33YY647 pKa = 7.96LTNLRR652 pKa = 11.84TIFGTDD658 pKa = 2.85TNLIKK663 pKa = 10.53AVHH666 pKa = 6.82PYY668 pKa = 9.41EE669 pKa = 4.91AYY671 pKa = 9.61PVSS674 pKa = 3.51

Molecular weight:
75.36 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|M9VTV7|M9VTV7_9VIRU RNA-dependent RNA polymerase OS=Crimson clover cryptic virus 2 OX=1323528 PE=4 SV=1
MM1 pKa = 7.81PFNAVRR7 pKa = 11.84NYY9 pKa = 10.52LNEE12 pKa = 3.53RR13 pKa = 11.84MTRR16 pKa = 11.84LKK18 pKa = 10.63QEE20 pKa = 3.51WKK22 pKa = 8.49TYY24 pKa = 10.3QSTDD28 pKa = 2.96HH29 pKa = 7.31DD30 pKa = 5.06PIKK33 pKa = 10.71ILDD36 pKa = 4.3TIQDD40 pKa = 3.59PDD42 pKa = 3.32YY43 pKa = 11.23RR44 pKa = 11.84RR45 pKa = 11.84YY46 pKa = 10.67LDD48 pKa = 3.33NARR51 pKa = 11.84FNSDD55 pKa = 3.17NEE57 pKa = 4.48MKK59 pKa = 10.57HH60 pKa = 6.7LMLNKK65 pKa = 9.91EE66 pKa = 3.89YY67 pKa = 9.61STLVEE72 pKa = 5.36AYY74 pKa = 8.28RR75 pKa = 11.84TDD77 pKa = 3.31NAKK80 pKa = 9.94KK81 pKa = 7.82HH82 pKa = 4.88QVYY85 pKa = 9.84EE86 pKa = 3.83LHH88 pKa = 6.59QPIPNDD94 pKa = 3.58AAPVIQSRR102 pKa = 11.84LPAKK106 pKa = 10.1GIKK109 pKa = 9.39LVPLMYY115 pKa = 10.13HH116 pKa = 6.17YY117 pKa = 11.45GHH119 pKa = 6.32VTHH122 pKa = 7.42DD123 pKa = 3.91PVSLTDD129 pKa = 4.02ANRR132 pKa = 11.84SDD134 pKa = 4.71DD135 pKa = 4.44FEE137 pKa = 4.61TQVVPDD143 pKa = 3.73SDD145 pKa = 3.55APTRR149 pKa = 11.84FGYY152 pKa = 10.0PIDD155 pKa = 3.56VRR157 pKa = 11.84IYY159 pKa = 10.33NLICYY164 pKa = 9.56RR165 pKa = 11.84YY166 pKa = 8.73SKK168 pKa = 10.59YY169 pKa = 11.01LEE171 pKa = 4.51VINAYY176 pKa = 9.52CRR178 pKa = 11.84PIGTVNATFEE188 pKa = 4.58DD189 pKa = 4.7FNKK192 pKa = 10.12EE193 pKa = 4.12QIPSAPIDD201 pKa = 3.67PKK203 pKa = 10.73RR204 pKa = 11.84KK205 pKa = 9.58EE206 pKa = 3.91NVLSHH211 pKa = 5.41IHH213 pKa = 6.52KK214 pKa = 10.53FLDD217 pKa = 3.5TKK219 pKa = 10.72PYY221 pKa = 10.62LPLHH225 pKa = 5.97FVDD228 pKa = 4.11TQFCKK233 pKa = 10.39TPLVTGTGYY242 pKa = 10.81HH243 pKa = 5.59NRR245 pKa = 11.84YY246 pKa = 9.47SFKK249 pKa = 10.71QKK251 pKa = 10.14AHH253 pKa = 6.25AKK255 pKa = 8.19YY256 pKa = 10.18SHH258 pKa = 6.48PAEE261 pKa = 4.48YY262 pKa = 8.59ATKK265 pKa = 9.37PISKK269 pKa = 10.03GYY271 pKa = 9.88FYY273 pKa = 11.23NATYY277 pKa = 10.76EE278 pKa = 3.93NARR281 pKa = 11.84TIIHH285 pKa = 6.83FIKK288 pKa = 10.54EE289 pKa = 3.72YY290 pKa = 10.52GLPFNVIRR298 pKa = 11.84ADD300 pKa = 3.92DD301 pKa = 3.98KK302 pKa = 11.95SEE304 pKa = 3.99LTDD307 pKa = 3.83SDD309 pKa = 3.74VQKK312 pKa = 11.1YY313 pKa = 10.08INEE316 pKa = 4.19ANSFFNDD323 pKa = 3.31YY324 pKa = 7.74PTLLFTRR331 pKa = 11.84NHH333 pKa = 5.47ISKK336 pKa = 10.27RR337 pKa = 11.84DD338 pKa = 3.55GPLKK342 pKa = 10.16VRR344 pKa = 11.84PVYY347 pKa = 10.62AVDD350 pKa = 4.65DD351 pKa = 3.68IFIIMEE357 pKa = 4.25LMLTFPLTIQARR369 pKa = 11.84KK370 pKa = 8.74PSCCIMYY377 pKa = 10.42GLEE380 pKa = 4.28TIRR383 pKa = 11.84GSNRR387 pKa = 11.84YY388 pKa = 8.8IEE390 pKa = 4.44QIARR394 pKa = 11.84DD395 pKa = 3.74YY396 pKa = 11.09STFFSLDD403 pKa = 2.74WSSYY407 pKa = 6.99DD408 pKa = 3.17QRR410 pKa = 11.84LPRR413 pKa = 11.84VITDD417 pKa = 3.3IYY419 pKa = 9.28YY420 pKa = 9.62TDD422 pKa = 4.27FLRR425 pKa = 11.84SLLVINNGYY434 pKa = 9.28QPTYY438 pKa = 10.24EE439 pKa = 4.22YY440 pKa = 8.37PTYY443 pKa = 10.44PDD445 pKa = 3.72LDD447 pKa = 3.61EE448 pKa = 5.24HH449 pKa = 6.91KK450 pKa = 10.3LYY452 pKa = 11.02HH453 pKa = 7.43RR454 pKa = 11.84MDD456 pKa = 3.79NLLHH460 pKa = 6.85FLHH463 pKa = 6.72LWYY466 pKa = 11.33NNMTFLLPDD475 pKa = 4.16GYY477 pKa = 11.15AYY479 pKa = 10.7RR480 pKa = 11.84RR481 pKa = 11.84TSCGVPSGLYY491 pKa = 7.56NTQYY495 pKa = 11.31LDD497 pKa = 3.35SFGNLFLIIDD507 pKa = 4.03AMLEE511 pKa = 3.96FGFTDD516 pKa = 3.75SEE518 pKa = 4.08IQKK521 pKa = 10.67FILLVLGDD529 pKa = 5.24DD530 pKa = 3.93NTGMTTIPICRR541 pKa = 11.84MFNFITFLEE550 pKa = 4.95KK551 pKa = 10.76YY552 pKa = 10.2ALEE555 pKa = 4.49RR556 pKa = 11.84YY557 pKa = 10.39NMVLSTTKK565 pKa = 10.58SVLTTFRR572 pKa = 11.84SKK574 pKa = 10.49IEE576 pKa = 3.95SLGYY580 pKa = 9.35QCNHH584 pKa = 6.66GSPKK588 pKa = 9.96RR589 pKa = 11.84DD590 pKa = 2.8IDD592 pKa = 3.69KK593 pKa = 11.03LVAQLCYY600 pKa = 9.98PEE602 pKa = 5.79NGLKK606 pKa = 9.45PHH608 pKa = 6.12TMAARR613 pKa = 11.84AIGIAYY619 pKa = 9.85ASAGQDD625 pKa = 3.3DD626 pKa = 4.87MFHH629 pKa = 6.69SFCQDD634 pKa = 2.32VYY636 pKa = 11.4NIFRR640 pKa = 11.84SDD642 pKa = 3.51YY643 pKa = 11.0RR644 pKa = 11.84PDD646 pKa = 3.55DD647 pKa = 3.83RR648 pKa = 11.84MNLHH652 pKa = 7.07FKK654 pKa = 10.58RR655 pKa = 11.84QIFHH659 pKa = 6.44NLEE662 pKa = 4.65DD663 pKa = 4.34GMPDD667 pKa = 3.93LAPPIVPPFPSLYY680 pKa = 10.08EE681 pKa = 3.62IRR683 pKa = 11.84EE684 pKa = 3.76MYY686 pKa = 10.25AYY688 pKa = 10.76YY689 pKa = 10.45KK690 pKa = 10.81GPLDD694 pKa = 5.61FAPKK698 pKa = 8.95WNYY701 pKa = 9.41AHH703 pKa = 6.86FMSDD707 pKa = 3.44PDD709 pKa = 3.77TTPPYY714 pKa = 10.32SKK716 pKa = 9.81TMRR719 pKa = 11.84DD720 pKa = 3.47YY721 pKa = 10.87EE722 pKa = 4.28AEE724 pKa = 4.06NNISSRR730 pKa = 11.84VAPTFEE736 pKa = 4.21TVVPSTINLPP746 pKa = 3.63

Molecular weight:
87.17 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1420

674

746

710.0

81.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.042 ± 1.352

1.127 ± 0.159

6.479 ± 0.766

4.225 ± 0.148

5.634 ± 0.103

3.31 ± 0.131

3.521 ± 0.127

5.915 ± 0.487

4.155 ± 0.8

8.944 ± 0.372

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.324 ± 0.366

6.127 ± 0.27

7.254 ± 0.211

2.676 ± 0.203

5.282 ± 0.26

6.761 ± 0.843

7.676 ± 0.726

5.0 ± 0.53

0.634 ± 0.073

5.915 ± 1.286

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski