Alienimonas californiensis

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Planctomycetales; Planctomycetaceae; Alienimonas

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4303 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A517P485|A0A517P485_9PLAN Uncharacterized protein OS=Alienimonas californiensis OX=2527989 GN=CA12_02660 PE=4 SV=1
MM1 pKa = 7.57LPPLKK6 pKa = 10.34RR7 pKa = 11.84HH8 pKa = 6.06ADD10 pKa = 3.71RR11 pKa = 11.84LRR13 pKa = 11.84RR14 pKa = 11.84AAVLGIVAGGAATPAFAGPPPCPPPAYY41 pKa = 9.9NPCPPAYY48 pKa = 9.64NPCPPGVITPVTPAPPAPMDD68 pKa = 4.08GSATEE73 pKa = 4.39LLPEE77 pKa = 4.42TFSRR81 pKa = 11.84TAPPSPAAQNLVANTANPLAGRR103 pKa = 11.84SGLAAVGGGVGDD115 pKa = 4.28PGYY118 pKa = 10.34IDD120 pKa = 3.82PAVVASRR127 pKa = 11.84FRR129 pKa = 11.84LRR131 pKa = 11.84YY132 pKa = 9.7SGARR136 pKa = 11.84DD137 pKa = 3.49GNSDD141 pKa = 3.43RR142 pKa = 11.84GEE144 pKa = 4.25FLYY147 pKa = 10.74GAYY150 pKa = 7.94DD151 pKa = 3.86TPALQASLPPGTPAVFIGPGNPGNVAAVPGQTVSGPTGPVRR192 pKa = 11.84PVIDD196 pKa = 3.84GFDD199 pKa = 3.54YY200 pKa = 11.54DD201 pKa = 4.66EE202 pKa = 4.51ISAYY206 pKa = 10.85LEE208 pKa = 4.18LEE210 pKa = 4.19VVEE213 pKa = 4.66GVSAFVEE220 pKa = 4.89VPWRR224 pKa = 11.84DD225 pKa = 3.17VSGYY229 pKa = 10.5DD230 pKa = 3.17QFRR233 pKa = 11.84DD234 pKa = 3.41QGFADD239 pKa = 5.35DD240 pKa = 5.32GLGDD244 pKa = 3.39INAGVRR250 pKa = 11.84VSLYY254 pKa = 10.62DD255 pKa = 3.88DD256 pKa = 4.97CDD258 pKa = 3.5TALTFQLMYY267 pKa = 9.04QTNTGDD273 pKa = 3.61PDD275 pKa = 3.88FGLGNGTQFIVPGLLFQQSLSDD297 pKa = 3.9DD298 pKa = 3.31LHH300 pKa = 8.08LFGEE304 pKa = 4.22AQYY307 pKa = 11.39YY308 pKa = 9.67VDD310 pKa = 4.81AGNGTEE316 pKa = 5.47FNGEE320 pKa = 4.4DD321 pKa = 3.59YY322 pKa = 11.0TSDD325 pKa = 3.18VLRR328 pKa = 11.84FGLGAALDD336 pKa = 4.02LVEE339 pKa = 4.79TRR341 pKa = 11.84DD342 pKa = 3.99TNLQLLNEE350 pKa = 4.31FVGWTFYY357 pKa = 11.07DD358 pKa = 4.09GQQSEE363 pKa = 4.95FNPATRR369 pKa = 11.84QIDD372 pKa = 3.66VTDD375 pKa = 4.21ADD377 pKa = 4.21GEE379 pKa = 4.71TIVNYY384 pKa = 10.24KK385 pKa = 10.27VGLRR389 pKa = 11.84YY390 pKa = 10.02LFGRR394 pKa = 11.84QSVYY398 pKa = 10.98AGYY401 pKa = 11.05GLALTDD407 pKa = 4.25DD408 pKa = 4.02VLYY411 pKa = 11.01DD412 pKa = 4.21DD413 pKa = 4.8IVRR416 pKa = 11.84LEE418 pKa = 3.89YY419 pKa = 10.75SVLLDD424 pKa = 3.45

Molecular weight: 44.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A517P9L4|A0A517P9L4_9PLAN Putative major pilin subunit OS=Alienimonas californiensis OX=2527989 GN=CA12_21610 PE=4 SV=1
MM1 pKa = 7.83AKK3 pKa = 8.26TQRR6 pKa = 11.84KK7 pKa = 8.43LKK9 pKa = 9.62KK10 pKa = 8.39AHH12 pKa = 6.49HH13 pKa = 6.56GSRR16 pKa = 11.84PASSKK21 pKa = 10.28ARR23 pKa = 11.84KK24 pKa = 8.05QKK26 pKa = 10.21RR27 pKa = 11.84RR28 pKa = 11.84AIKK31 pKa = 8.92TT32 pKa = 3.46

Molecular weight: 3.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4303

0

4303

1597559

30

4167

371.3

39.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.874 ± 0.068

1.001 ± 0.015

6.343 ± 0.026

6.29 ± 0.035

3.323 ± 0.022

9.349 ± 0.04

1.899 ± 0.019

2.949 ± 0.023

2.479 ± 0.031

10.165 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.556 ± 0.017

2.221 ± 0.021

6.925 ± 0.041

2.491 ± 0.019

7.911 ± 0.041

4.801 ± 0.023

5.394 ± 0.026

7.58 ± 0.033

1.486 ± 0.016

1.963 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski