Methylotenera mobilis (strain JLW8 / ATCC BAA-1282 / DSM 17540)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Methylophilaceae; Methylotenera; Methylotenera mobilis

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2334 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|C6WV09|C6WV09_METML Uncharacterized protein OS=Methylotenera mobilis (strain JLW8 / ATCC BAA-1282 / DSM 17540) OX=583345 GN=Mmol_0848 PE=4 SV=1
MM1 pKa = 7.48ALIGKK6 pKa = 8.81IIAMTGTASLISNNGNQRR24 pKa = 11.84DD25 pKa = 4.01LRR27 pKa = 11.84LGDD30 pKa = 4.37NIQTADD36 pKa = 4.0TIKK39 pKa = 9.69TGAGVEE45 pKa = 4.08VDD47 pKa = 3.67LQLANGQVVHH57 pKa = 7.02IGAEE61 pKa = 3.99QLVAFADD68 pKa = 3.88EE69 pKa = 4.34LVEE72 pKa = 4.54DD73 pKa = 5.74FIPATLDD80 pKa = 3.1TSVNVATIDD89 pKa = 3.78TVVKK93 pKa = 10.57AIEE96 pKa = 4.08EE97 pKa = 4.33GKK99 pKa = 10.59DD100 pKa = 2.94IGDD103 pKa = 3.76VLEE106 pKa = 4.44EE107 pKa = 4.03TAAGPGGSSNSYY119 pKa = 9.86GFSFVDD125 pKa = 3.93LLRR128 pKa = 11.84INDD131 pKa = 3.93DD132 pKa = 3.65LNNFRR137 pKa = 11.84FGYY140 pKa = 9.6EE141 pKa = 4.14FGSATPNEE149 pKa = 4.2QALSGNSFADD159 pKa = 3.34TLNQINVVTGTGGAPDD175 pKa = 4.01TNSPSVVVNIVDD187 pKa = 3.67ANLNVADD194 pKa = 4.16TVSNVTFTFSEE205 pKa = 4.6APVGFTIGDD214 pKa = 3.37ISSTGGIISNLVATADD230 pKa = 3.85PLVFTATFTANAGFEE245 pKa = 4.54GTGSVSVNAGSYY257 pKa = 9.55TNASGNVGTSGADD270 pKa = 3.08TVGIDD275 pKa = 3.36TAAPVPTITLTPNVTADD292 pKa = 4.06DD293 pKa = 4.6IINAGEE299 pKa = 3.88AGGNVNITGTVGGDD313 pKa = 2.91AKK315 pKa = 11.58VNDD318 pKa = 4.13TVTLVINGNTYY329 pKa = 9.85TGLVQPGLTFSIPVAGSDD347 pKa = 4.57LVADD351 pKa = 4.45PDD353 pKa = 3.89RR354 pKa = 11.84VIDD357 pKa = 4.08ASVSTTDD364 pKa = 3.63AAGNTATASDD374 pKa = 4.0TEE376 pKa = 4.74GYY378 pKa = 10.15SVDD381 pKa = 3.54STVPTIANQTFSYY394 pKa = 10.76AEE396 pKa = 3.76NRR398 pKa = 11.84AAGATVATVVASDD411 pKa = 3.54NVGVTGYY418 pKa = 10.88SFTATGTNTSADD430 pKa = 3.41GYY432 pKa = 8.73YY433 pKa = 10.11QISNTGVITITAAGAASGVNDD454 pKa = 4.43FEE456 pKa = 5.1QGTNTGNYY464 pKa = 9.24GITVRR469 pKa = 11.84DD470 pKa = 3.3AAGNNSNATITLNEE484 pKa = 4.07TDD486 pKa = 5.47LDD488 pKa = 3.89DD489 pKa = 4.5TNPVIANQTFSYY501 pKa = 10.76AEE503 pKa = 3.76NRR505 pKa = 11.84AAGATVATVVASDD518 pKa = 3.54NVGVTGYY525 pKa = 10.88SFTATGTNTSADD537 pKa = 3.41GYY539 pKa = 8.73YY540 pKa = 10.11QISNTGVITITAAGAASGVNDD561 pKa = 4.43FEE563 pKa = 5.1QGTNTGNYY571 pKa = 9.24GITVRR576 pKa = 11.84DD577 pKa = 3.3AAGNNSNATITLNEE591 pKa = 4.25TNVNEE596 pKa = 3.96PVAAVSDD603 pKa = 4.45AYY605 pKa = 10.0TMNEE609 pKa = 4.33DD610 pKa = 3.63GPAITLTPLANDD622 pKa = 3.81SDD624 pKa = 4.89PDD626 pKa = 3.64GTTPTIQSINGTALTPGVAQAIAVTGGMVNVSAAGVITFTPTLNFNGTVTFPYY679 pKa = 10.36VITDD683 pKa = 4.07GATTATANQVITVNAVDD700 pKa = 4.61DD701 pKa = 4.48PSVLIADD708 pKa = 4.11TNTVNEE714 pKa = 4.77DD715 pKa = 3.15NPATGNVLSNDD726 pKa = 3.29SDD728 pKa = 3.66VDD730 pKa = 3.66NVLTVSSFKK739 pKa = 10.88VAGISGTFTAGQTATITGVGTLTIAANGNYY769 pKa = 9.01TFTPVANYY777 pKa = 9.91NGSVPVATYY786 pKa = 8.82TVNTGSTSTLSITVTPVNDD805 pKa = 3.72APVGVADD812 pKa = 4.07VFNGVNSVVEE822 pKa = 4.15GTTVVRR828 pKa = 11.84GNILANDD835 pKa = 3.95TDD837 pKa = 3.85VDD839 pKa = 4.26STTLTVAQFATNSGATATAVNGTNSITTALGGTVVMNADD878 pKa = 3.3GTFTYY883 pKa = 7.9TAPVRR888 pKa = 11.84NHH890 pKa = 6.55ADD892 pKa = 3.73AISDD896 pKa = 3.27VDD898 pKa = 3.97SFVYY902 pKa = 10.3RR903 pKa = 11.84ATDD906 pKa = 3.57GSLNSAWTTVTLNIADD922 pKa = 4.25SVPVANPDD930 pKa = 3.4TDD932 pKa = 3.84SVGVGFRR939 pKa = 11.84NPTTNNTASVTGNVITGAGDD959 pKa = 3.2TGGVDD964 pKa = 3.43TLGADD969 pKa = 3.7AARR972 pKa = 11.84VSSIVFNGTTYY983 pKa = 11.29NLSAGNTSIVTSNGTLLINEE1003 pKa = 4.54TGSYY1007 pKa = 9.6TYY1009 pKa = 10.42TSSYY1013 pKa = 8.19QNKK1016 pKa = 8.23VVPVSPSNTASTVANWTSAGISSFGFDD1043 pKa = 3.08GTSPLTNSNNTLTLSALTTAASNIVRR1069 pKa = 11.84LSDD1072 pKa = 3.38NTNSNNDD1079 pKa = 3.94GIGVEE1084 pKa = 4.48TTLSGSASNQSNISRR1099 pKa = 11.84IEE1101 pKa = 4.08NNEE1104 pKa = 3.9HH1105 pKa = 6.54LVLNLGMLSRR1115 pKa = 11.84NTSVTLTDD1123 pKa = 3.82LTASEE1128 pKa = 4.31TAQWRR1133 pKa = 11.84AYY1135 pKa = 9.19DD1136 pKa = 3.6ANGAIVASGTITGNSTNIATGTVSSTVAFQYY1167 pKa = 10.64IVFSGSSSANFRR1179 pKa = 11.84VNGLTATPDD1188 pKa = 3.54LTAVTPDD1195 pKa = 2.85QFTYY1199 pKa = 10.78TLTDD1203 pKa = 3.44ADD1205 pKa = 4.34GSTASTTLTINTNGNPSAVADD1226 pKa = 3.66SATVYY1231 pKa = 10.71EE1232 pKa = 4.81SGLSTGTQAGVLATTASGNLLVNDD1256 pKa = 4.8AGVSTTTTISSINGVTPTSGTITVTSATGTLVVNANTGAYY1296 pKa = 8.22TYY1298 pKa = 9.38TLNAATTEE1306 pKa = 4.19GVNDD1310 pKa = 3.96KK1311 pKa = 9.18PTFNYY1316 pKa = 10.29VLTDD1320 pKa = 3.51SVTGQSTNANLTVNIVDD1337 pKa = 4.23DD1338 pKa = 4.82APVGGNITQTLQAASAALTYY1358 pKa = 10.91NVVIVLDD1365 pKa = 4.22RR1366 pKa = 11.84SGSMAQDD1373 pKa = 3.95ANGLWSNQSGYY1384 pKa = 10.63DD1385 pKa = 3.17ASTNRR1390 pKa = 11.84MEE1392 pKa = 4.27IAKK1395 pKa = 9.79EE1396 pKa = 3.91AIAQLIARR1404 pKa = 11.84YY1405 pKa = 9.86DD1406 pKa = 3.39GLGNVNVKK1414 pKa = 9.95FVTFSSDD1421 pKa = 2.98AVEE1424 pKa = 4.33SEE1426 pKa = 4.06WYY1428 pKa = 9.39IDD1430 pKa = 3.59NVTGAVRR1437 pKa = 11.84YY1438 pKa = 9.54VDD1440 pKa = 3.69NVQAGGGTQYY1450 pKa = 10.0STALNEE1456 pKa = 4.26TMSGFTQPVADD1467 pKa = 3.44KK1468 pKa = 10.02TLFYY1472 pKa = 10.46FITDD1476 pKa = 4.05GQPNSGYY1483 pKa = 10.39EE1484 pKa = 3.87VDD1486 pKa = 5.13ATLQTQWQNFVAANGNISFGIGIGTASLSSLTPIAYY1522 pKa = 9.22PNVDD1526 pKa = 3.02ADD1528 pKa = 4.18GNGTEE1533 pKa = 5.01DD1534 pKa = 3.4YY1535 pKa = 10.6AIRR1538 pKa = 11.84VDD1540 pKa = 4.2NPADD1544 pKa = 3.65LADD1547 pKa = 4.13TLLSTVDD1554 pKa = 3.29GGVVVGNMSVLSGSGSSGFLLGADD1578 pKa = 3.9GGRR1581 pKa = 11.84LNSVVVDD1588 pKa = 4.16GVTYY1592 pKa = 9.21TYY1594 pKa = 10.66SASGPSSMTISTNKK1608 pKa = 10.24GGEE1611 pKa = 4.04LTVNFLTGEE1620 pKa = 4.08YY1621 pKa = 9.93SYY1623 pKa = 11.6HH1624 pKa = 6.58LLLNRR1629 pKa = 11.84TVQNEE1634 pKa = 4.1QEE1636 pKa = 4.5SFLVTAVDD1644 pKa = 3.76GDD1646 pKa = 4.23GDD1648 pKa = 4.04TKK1650 pKa = 10.85TINLNINLDD1659 pKa = 3.81YY1660 pKa = 11.02VANLDD1665 pKa = 4.02PNADD1669 pKa = 3.98TILTNVVTGTPISVSAAALLHH1690 pKa = 6.05NDD1692 pKa = 3.52SLRR1695 pKa = 11.84GTGSITSTQNAVNGSVSGTSTVQFTANAVAAKK1727 pKa = 8.33TIQVVTEE1734 pKa = 3.99ASFDD1738 pKa = 3.94STSQTQNSTRR1748 pKa = 11.84EE1749 pKa = 4.0NAVDD1753 pKa = 3.35LTDD1756 pKa = 3.52RR1757 pKa = 11.84SKK1759 pKa = 11.2FGTVVSGTPTWAVDD1773 pKa = 3.49VAGGSSIVFSGLLDD1787 pKa = 3.55NSGSGTRR1794 pKa = 11.84DD1795 pKa = 2.64VDD1797 pKa = 3.82YY1798 pKa = 11.52VKK1800 pKa = 11.12VKK1802 pKa = 9.21MYY1804 pKa = 10.46AGEE1807 pKa = 4.09RR1808 pKa = 11.84VFIDD1812 pKa = 4.19VDD1814 pKa = 3.58NQTQGVNAFVEE1825 pKa = 4.46YY1826 pKa = 10.46FDD1828 pKa = 5.65ANGVLQTFTVGTTGTGTSLAPNGYY1852 pKa = 8.02FTAPEE1857 pKa = 3.9SGEE1860 pKa = 3.88YY1861 pKa = 9.55FIRR1864 pKa = 11.84MQTVGTTDD1872 pKa = 2.79TSYY1875 pKa = 11.96NLVLTLTDD1883 pKa = 2.9IVGPMTEE1890 pKa = 4.29NGSFEE1895 pKa = 4.3YY1896 pKa = 10.26TVTEE1900 pKa = 3.95NGVASSASATVYY1912 pKa = 10.5NVVGNTITGGDD1923 pKa = 3.65GDD1925 pKa = 4.83EE1926 pKa = 4.6ILLGGNTNDD1935 pKa = 3.64TLIGGGGNDD1944 pKa = 3.64VLIGGAGNDD1953 pKa = 3.71TLLGGTGADD1962 pKa = 3.34RR1963 pKa = 11.84LEE1965 pKa = 4.64GGSGNDD1971 pKa = 3.32ILNGGTGNDD1980 pKa = 3.21ILIGGTGNDD1989 pKa = 3.89TLTGGLGADD1998 pKa = 3.47VFKK2001 pKa = 10.84WSLADD2006 pKa = 3.89AGATGTPATDD2016 pKa = 3.57VITDD2020 pKa = 4.32FDD2022 pKa = 3.84TTANSDD2028 pKa = 3.72KK2029 pKa = 11.21LDD2031 pKa = 4.69LRR2033 pKa = 11.84DD2034 pKa = 4.03LLQGEE2039 pKa = 4.48IGSGVGANLEE2049 pKa = 4.27NYY2051 pKa = 10.32LHH2053 pKa = 6.44FEE2055 pKa = 4.12KK2056 pKa = 10.91VGTDD2060 pKa = 3.39TVVHH2064 pKa = 6.59ISSNGSFNNGYY2075 pKa = 9.73NPAAEE2080 pKa = 4.3VQTITLQNVDD2090 pKa = 4.11LVGSYY2095 pKa = 11.52ANDD2098 pKa = 3.22QQVIQNLINNQKK2110 pKa = 10.63LITDD2114 pKa = 4.23

Molecular weight:
214.9 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|C6WTX1|C6WTX1_METML Uncharacterized protein OS=Methylotenera mobilis (strain JLW8 / ATCC BAA-1282 / DSM 17540) OX=583345 GN=Mmol_0460 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 7.79TRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.79GFLVRR21 pKa = 11.84MKK23 pKa = 9.4TKK25 pKa = 10.36GGRR28 pKa = 11.84AVIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.15GRR39 pKa = 11.84SRR41 pKa = 11.84LGLL44 pKa = 3.62

Molecular weight:
5.03 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2334

0

2334

757536

23

2787

324.6

35.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.12 ± 0.061

0.868 ± 0.019

5.273 ± 0.041

5.659 ± 0.046

3.809 ± 0.035

6.82 ± 0.058

2.329 ± 0.029

6.3 ± 0.044

5.375 ± 0.044

10.506 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.618 ± 0.025

4.353 ± 0.046

4.033 ± 0.032

4.371 ± 0.038

4.635 ± 0.037

6.22 ± 0.039

5.517 ± 0.046

7.202 ± 0.041

1.127 ± 0.021

2.864 ± 0.029

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski