Roseobacter phage RDJL Phi 1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Xiamenvirus; Roseobacter virus RDJL1

Average proteome isoelectric point is 5.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 87 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F4YXM0|F4YXM0_9CAUD Uncharacterized protein OS=Roseobacter phage RDJL Phi 1 OX=562742 GN=RDJLphi1_gp09 PE=4 SV=1
MM1 pKa = 5.91THH3 pKa = 5.26VWINGIRR10 pKa = 11.84HH11 pKa = 5.36YY12 pKa = 10.25PAEE15 pKa = 4.4PLDD18 pKa = 3.95EE19 pKa = 4.7EE20 pKa = 5.15GVAEE24 pKa = 4.96LIEE27 pKa = 4.66EE28 pKa = 4.51GWDD31 pKa = 3.48DD32 pKa = 5.13QLILLDD38 pKa = 4.02GKK40 pKa = 10.06PYY42 pKa = 9.63DD43 pKa = 4.39TEE45 pKa = 5.18DD46 pKa = 3.3LCQCSSCEE54 pKa = 5.24AFFTWRR60 pKa = 11.84GDD62 pKa = 3.47LDD64 pKa = 5.39DD65 pKa = 5.1EE66 pKa = 5.73LEE68 pKa = 4.77CEE70 pKa = 4.32DD71 pKa = 4.21CAEE74 pKa = 4.5EE75 pKa = 4.11SRR77 pKa = 11.84QAAEE81 pKa = 3.98EE82 pKa = 3.96QRR84 pKa = 11.84DD85 pKa = 3.85LEE87 pKa = 4.41SWARR91 pKa = 11.84WACRR95 pKa = 3.22

Molecular weight: 11.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F4YXR5|F4YXR5_9CAUD Uncharacterized protein OS=Roseobacter phage RDJL Phi 1 OX=562742 GN=RDJLphi1_gp54 PE=4 SV=1
MM1 pKa = 7.65EE2 pKa = 4.67LTGKK6 pKa = 9.65NNSWGIKK13 pKa = 9.26SGLIGLHH20 pKa = 4.66PAKK23 pKa = 10.65RR24 pKa = 11.84NGLVVKK30 pKa = 10.52NRR32 pKa = 11.84VRR34 pKa = 11.84IIGQVPAGSWPQGFRR49 pKa = 11.84GFMAPATGKK58 pKa = 8.24TPNSCLLYY66 pKa = 10.45LFPEE70 pKa = 4.23SHH72 pKa = 6.8LSQWVSDD79 pKa = 3.99GGSKK83 pKa = 10.37RR84 pKa = 11.84SPDD87 pKa = 3.3QGVTFQVQDD96 pKa = 3.41TLRR99 pKa = 11.84FSVYY103 pKa = 8.95RR104 pKa = 11.84HH105 pKa = 4.89SHH107 pKa = 5.58HH108 pKa = 7.04PVVQALRR115 pKa = 11.84LATKK119 pKa = 9.67MDD121 pKa = 4.23FFGHH125 pKa = 5.17TRR127 pKa = 11.84TRR129 pKa = 11.84YY130 pKa = 9.2HH131 pKa = 6.58VLLDD135 pKa = 3.98GDD137 pKa = 4.22SMTIRR142 pKa = 11.84VPEE145 pKa = 4.25RR146 pKa = 11.84EE147 pKa = 4.41DD148 pKa = 3.1IQPYY152 pKa = 8.19RR153 pKa = 11.84QYY155 pKa = 10.83RR156 pKa = 11.84QRR158 pKa = 11.84DD159 pKa = 3.53TSQGRR164 pKa = 11.84LFF166 pKa = 4.03

Molecular weight: 18.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

87

0

87

18783

57

1361

215.9

23.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.86 ± 0.491

1.059 ± 0.138

7.086 ± 0.261

7.501 ± 0.338

3.7 ± 0.174

7.879 ± 0.233

1.901 ± 0.159

4.461 ± 0.131

4.802 ± 0.351

8.524 ± 0.232

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.598 ± 0.184

3.423 ± 0.189

4.536 ± 0.216

3.487 ± 0.173

6.218 ± 0.282

5.462 ± 0.377

6.671 ± 0.398

6.41 ± 0.197

1.922 ± 0.171

2.497 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski