Postia placenta (strain ATCC 44394 / Madison 698-R) (Brown rot fungus) (Poria monticola)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Polyporales; Dacryobolaceae; Postia; Postia placenta

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8983 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B8PC80|B8PC80_POSPM Predicted protein OS=Postia placenta (strain ATCC 44394 / Madison 698-R) OX=561896 GN=POSPLDRAFT_102490 PE=4 SV=1
MM1 pKa = 7.63EE2 pKa = 5.69PMDD5 pKa = 4.38VDD7 pKa = 3.16WDD9 pKa = 3.77AAQEE13 pKa = 4.03ARR15 pKa = 11.84HH16 pKa = 6.02EE17 pKa = 4.14PMQIDD22 pKa = 4.46CIGSFKK28 pKa = 10.85YY29 pKa = 10.18DD30 pKa = 3.2VSMDD34 pKa = 3.22WSTQDD39 pKa = 2.97YY40 pKa = 7.49TANDD44 pKa = 3.7GCTATKK50 pKa = 10.58SDD52 pKa = 3.61TDD54 pKa = 4.87AILSPCTAIINVVGLPSPHH73 pKa = 6.88GYY75 pKa = 10.74GPIADD80 pKa = 4.09AANEE84 pKa = 4.35GFQSWWFEE92 pKa = 4.19TVSAAGTPGSAATQDD107 pKa = 4.36FISGGTVLNLFKK119 pKa = 11.16QNIFQLDD126 pKa = 3.48LRR128 pKa = 11.84NTQGYY133 pKa = 8.82NPPEE137 pKa = 4.13PLL139 pKa = 3.86

Molecular weight: 15.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B8PKB1|B8PKB1_POSPM Predicted protein (Fragment) OS=Postia placenta (strain ATCC 44394 / Madison 698-R) OX=561896 GN=POSPLDRAFT_18999 PE=4 SV=1
PP1 pKa = 7.61FSLLKK6 pKa = 10.05LTTTSPILSVVQQVRR21 pKa = 11.84FRR23 pKa = 11.84SRR25 pKa = 11.84GTEE28 pKa = 3.83YY29 pKa = 10.71QPSQRR34 pKa = 11.84KK35 pKa = 8.85RR36 pKa = 11.84KK37 pKa = 9.17RR38 pKa = 11.84KK39 pKa = 9.56HH40 pKa = 5.55GFLARR45 pKa = 11.84KK46 pKa = 9.65RR47 pKa = 11.84SVSGQKK53 pKa = 9.58ILVRR57 pKa = 11.84RR58 pKa = 11.84RR59 pKa = 11.84AKK61 pKa = 9.8GRR63 pKa = 11.84RR64 pKa = 11.84FLSHH68 pKa = 6.87

Molecular weight: 8.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8983

0

8983

3900308

27

3369

434.2

47.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.461 ± 0.027

1.487 ± 0.011

5.785 ± 0.02

5.939 ± 0.025

3.299 ± 0.014

6.377 ± 0.024

2.716 ± 0.012

4.526 ± 0.02

3.99 ± 0.024

9.067 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.1 ± 0.009

3.038 ± 0.012

6.582 ± 0.03

3.704 ± 0.014

7.027 ± 0.026

8.299 ± 0.03

6.058 ± 0.017

6.452 ± 0.019

1.533 ± 0.009

2.559 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski