Methylovorus glucosetrophus (strain SIP3-4)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Methylophilaceae; Methylovorus; Methylovorus glucosotrophus

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2907 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C6XBQ3|C6XBQ3_METGS Acyl carrier protein-related protein OS=Methylovorus glucosetrophus (strain SIP3-4) OX=582744 GN=Msip34_2786 PE=4 SV=1
MM1 pKa = 7.52AKK3 pKa = 8.77STSFDD8 pKa = 3.59DD9 pKa = 3.76YY10 pKa = 10.67VTYY13 pKa = 10.89LIDD16 pKa = 3.62TEE18 pKa = 4.51EE19 pKa = 4.65NDD21 pKa = 3.61TASASLGKK29 pKa = 7.45TTITVSLAGLTDD41 pKa = 3.55NSQQEE46 pKa = 3.92AAARR50 pKa = 11.84HH51 pKa = 5.61ALEE54 pKa = 4.21AWEE57 pKa = 4.91DD58 pKa = 3.63ATGLTFTIVTSGSADD73 pKa = 2.97IYY75 pKa = 10.73IGNDD79 pKa = 3.2DD80 pKa = 4.29ALGEE84 pKa = 3.94GAYY87 pKa = 9.99AYY89 pKa = 9.74EE90 pKa = 4.53EE91 pKa = 3.81NDD93 pKa = 2.8ARR95 pKa = 11.84YY96 pKa = 10.32INVSEE101 pKa = 3.81NWMDD105 pKa = 3.99GFPADD110 pKa = 4.25EE111 pKa = 4.03QWSVGSYY118 pKa = 9.81GVQTFIHH125 pKa = 6.8EE126 pKa = 4.31IGHH129 pKa = 5.17TLGLGHH135 pKa = 7.29GGPYY139 pKa = 9.97NGNGSYY145 pKa = 11.03AKK147 pKa = 10.36DD148 pKa = 3.47AIFALDD154 pKa = 3.55TNQYY158 pKa = 11.1SIMSYY163 pKa = 10.65FDD165 pKa = 2.99QSNYY169 pKa = 10.35GGASNLFLLSPMMADD184 pKa = 3.26IAAIRR189 pKa = 11.84LLYY192 pKa = 10.85GNLATNTGDD201 pKa = 3.2TLYY204 pKa = 11.45GLGEE208 pKa = 4.3TVLGGTSDD216 pKa = 3.24MGSYY220 pKa = 10.36AGYY223 pKa = 10.83ARR225 pKa = 11.84TISDD229 pKa = 3.42SDD231 pKa = 3.67GTDD234 pKa = 2.71TMDD237 pKa = 4.05FSNVSRR243 pKa = 11.84GSVIDD248 pKa = 3.4MRR250 pKa = 11.84AGGFSSINGYY260 pKa = 9.75KK261 pKa = 10.33KK262 pKa = 10.56NFAIAQDD269 pKa = 3.76TTIEE273 pKa = 4.17NLNATLGRR281 pKa = 11.84DD282 pKa = 3.34TVTGNDD288 pKa = 4.11ADD290 pKa = 3.85NLIYY294 pKa = 10.65GLSGNDD300 pKa = 4.09KK301 pKa = 10.14ISSGAGNDD309 pKa = 3.72TLDD312 pKa = 4.03GGLGGDD318 pKa = 4.07TLTGGLGDD326 pKa = 3.86DD327 pKa = 3.7VYY329 pKa = 11.41YY330 pKa = 10.45INSSKK335 pKa = 11.13DD336 pKa = 3.59VISEE340 pKa = 4.19KK341 pKa = 10.88ADD343 pKa = 3.24GGTDD347 pKa = 3.25VVYY350 pKa = 11.02ASVSYY355 pKa = 10.59TMASYY360 pKa = 10.96LEE362 pKa = 4.21EE363 pKa = 4.1VVLQGSASTKK373 pKa = 10.62LKK375 pKa = 9.79ATGNAMANQITGDD388 pKa = 3.7DD389 pKa = 3.9GANVLNGGAGNDD401 pKa = 3.72TLAGGLGRR409 pKa = 11.84DD410 pKa = 3.7TLAGGTGNDD419 pKa = 3.83SLDD422 pKa = 3.95GGDD425 pKa = 5.51ANDD428 pKa = 4.7KK429 pKa = 10.92LDD431 pKa = 4.31GGAGDD436 pKa = 3.94DD437 pKa = 4.16TLVGGAGNDD446 pKa = 3.81LLKK449 pKa = 11.08GGAGSDD455 pKa = 2.99IFIIEE460 pKa = 5.36DD461 pKa = 3.73GTDD464 pKa = 2.94HH465 pKa = 8.02DD466 pKa = 5.18IISDD470 pKa = 3.97FAYY473 pKa = 10.5SSKK476 pKa = 9.46TSKK479 pKa = 11.08GITTIVEE486 pKa = 3.96DD487 pKa = 3.36KK488 pKa = 11.12LYY490 pKa = 9.45ITGHH494 pKa = 7.11DD495 pKa = 3.88YY496 pKa = 11.43DD497 pKa = 4.33QDD499 pKa = 3.97TLFDD503 pKa = 4.55GDD505 pKa = 4.54DD506 pKa = 3.79YY507 pKa = 11.98SVGLAADD514 pKa = 3.87DD515 pKa = 4.17SGLLFSFYY523 pKa = 10.89SGGSLLLKK531 pKa = 10.84GIGEE535 pKa = 4.35SMLDD539 pKa = 3.38KK540 pKa = 10.87FDD542 pKa = 3.53IAALHH547 pKa = 6.0TDD549 pKa = 3.76EE550 pKa = 6.1NGDD553 pKa = 3.86SYY555 pKa = 11.75FSLAA559 pKa = 4.01

Molecular weight: 58.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C6XAU7|C6XAU7_METGS Efflux transporter RND family MFP subunit OS=Methylovorus glucosetrophus (strain SIP3-4) OX=582744 GN=Msip34_2480 PE=3 SV=1
MM1 pKa = 7.21MPLHH5 pKa = 6.49RR6 pKa = 11.84PWRR9 pKa = 11.84LIRR12 pKa = 11.84GCRR15 pKa = 11.84RR16 pKa = 11.84AWLAMLGQAMLVFSMASAHH35 pKa = 6.22AAADD39 pKa = 3.46AHH41 pKa = 6.55VEE43 pKa = 3.88SHH45 pKa = 5.43YY46 pKa = 11.12RR47 pKa = 11.84EE48 pKa = 3.88AGYY51 pKa = 10.34FIGDD55 pKa = 3.82VIDD58 pKa = 3.87EE59 pKa = 4.52VVDD62 pKa = 3.51ITATPGATLVEE73 pKa = 4.38EE74 pKa = 4.22TLPRR78 pKa = 11.84SGDD81 pKa = 3.75GQWIEE86 pKa = 3.79LRR88 pKa = 11.84EE89 pKa = 4.14VNVQEE94 pKa = 4.15NAPEE98 pKa = 3.96HH99 pKa = 6.44GRR101 pKa = 11.84FKK103 pKa = 11.17LHH105 pKa = 6.99LRR107 pKa = 11.84WQLFKK112 pKa = 11.06ALRR115 pKa = 11.84EE116 pKa = 4.09TRR118 pKa = 11.84TIPLPAFTVNLRR130 pKa = 11.84HH131 pKa = 5.88GQTVLPVKK139 pKa = 9.64IHH141 pKa = 5.73EE142 pKa = 4.36RR143 pKa = 11.84QVMVSPLLPVILDD156 pKa = 3.89TSQTSLRR163 pKa = 11.84PDD165 pKa = 3.41SLPPLRR171 pKa = 11.84DD172 pKa = 3.1TLRR175 pKa = 11.84PMRR178 pKa = 11.84HH179 pKa = 4.85TVLALALTLSAALWLLWRR197 pKa = 11.84YY198 pKa = 8.71DD199 pKa = 3.55KK200 pKa = 11.4PPFRR204 pKa = 11.84WAAAGPFTAACRR216 pKa = 11.84RR217 pKa = 11.84LRR219 pKa = 11.84GRR221 pKa = 11.84QGRR224 pKa = 11.84KK225 pKa = 9.49LSLTQSFAVAHH236 pKa = 6.13HH237 pKa = 7.08AFNQAAGRR245 pKa = 11.84TVFASDD251 pKa = 3.7VPVLLKK257 pKa = 10.63LRR259 pKa = 11.84PEE261 pKa = 4.48LARR264 pKa = 11.84LRR266 pKa = 11.84PEE268 pKa = 3.54IEE270 pKa = 3.62AFYY273 pKa = 10.97ARR275 pKa = 11.84SARR278 pKa = 11.84VFFAEE283 pKa = 4.47APNAASADD291 pKa = 3.51DD292 pKa = 3.7VAAIKK297 pKa = 9.5TLLAACRR304 pKa = 11.84ALEE307 pKa = 4.13RR308 pKa = 11.84GRR310 pKa = 11.84KK311 pKa = 7.45PP312 pKa = 3.17

Molecular weight: 35.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2907

0

2907

933751

23

2907

321.2

35.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.494 ± 0.057

0.822 ± 0.015

5.379 ± 0.036

5.746 ± 0.044

3.643 ± 0.029

7.297 ± 0.053

2.385 ± 0.029

5.727 ± 0.034

4.488 ± 0.041

10.682 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.689 ± 0.022

3.623 ± 0.039

4.544 ± 0.033

4.54 ± 0.035

5.563 ± 0.043

6.004 ± 0.041

5.285 ± 0.042

6.953 ± 0.039

1.301 ± 0.021

2.837 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski