Pseudomonas phage phi297

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H2BD49|H2BD49_9CAUD Putative RecT protein OS=Pseudomonas phage phi297 OX=1129145 GN=phi297_00014 PE=4 SV=1
MM1 pKa = 7.79PSLTVLEE8 pKa = 4.89RR9 pKa = 11.84YY10 pKa = 9.32GQVGEE15 pKa = 4.4FAALLGAAEE24 pKa = 4.67LNAATDD30 pKa = 3.14WDD32 pKa = 4.22EE33 pKa = 4.82QFLADD38 pKa = 5.04LRR40 pKa = 11.84SNFQRR45 pKa = 11.84YY46 pKa = 7.58GAHH49 pKa = 6.6TYY51 pKa = 10.99LSDD54 pKa = 3.57AQLEE58 pKa = 4.19QLEE61 pKa = 4.73RR62 pKa = 11.84IANEE66 pKa = 3.68

Molecular weight: 7.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H2BD37|H2BD37_9CAUD Uncharacterized protein OS=Pseudomonas phage phi297 OX=1129145 GN=phi297_0002 PE=4 SV=1
MM1 pKa = 8.26PDD3 pKa = 3.08RR4 pKa = 11.84SHH6 pKa = 7.31AGGLAGGVLRR16 pKa = 11.84MKK18 pKa = 10.09QKK20 pKa = 10.6PGIALPRR27 pKa = 11.84WLLRR31 pKa = 11.84TTTMQMHH38 pKa = 6.08SVDD41 pKa = 3.95VVLVMALVLQHH52 pKa = 6.9HH53 pKa = 6.34GTADD57 pKa = 3.38AVRR60 pKa = 11.84RR61 pKa = 11.84AAGQLRR67 pKa = 11.84DD68 pKa = 3.81RR69 pKa = 11.84VCAEE73 pKa = 4.33HH74 pKa = 6.63RR75 pKa = 11.84PKK77 pKa = 8.65MTALMRR83 pKa = 11.84MQDD86 pKa = 3.47DD87 pKa = 3.62AAALQVALNIVQRR100 pKa = 11.84ATDD103 pKa = 3.61ALGILPGTPFPAGPVLTNRR122 pKa = 11.84YY123 pKa = 9.9LEE125 pKa = 4.3TQLAPGPYY133 pKa = 7.79QWTRR137 pKa = 11.84EE138 pKa = 4.05VSLCATPSSTKK149 pKa = 9.9TSPASSTT156 pKa = 3.41

Molecular weight: 16.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

69

0

69

15578

40

1003

225.8

24.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.132 ± 0.683

1.085 ± 0.112

5.797 ± 0.218

6.188 ± 0.305

2.882 ± 0.185

7.902 ± 0.233

2.022 ± 0.174

4.802 ± 0.233

3.755 ± 0.22

8.717 ± 0.364

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.407 ± 0.16

3.21 ± 0.247

5.026 ± 0.271

4.506 ± 0.281

7.209 ± 0.386

5.854 ± 0.262

5.514 ± 0.24

6.58 ± 0.288

1.592 ± 0.14

2.818 ± 0.215
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski