Capsaspora owczarzaki (strain ATCC 30864)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Filasterea; Capsaspora; Capsaspora owczarzaki

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9794 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D2VYG0|A0A0D2VYG0_CAPO3 DNA-directed DNA polymerase OS=Capsaspora owczarzaki (strain ATCC 30864) OX=595528 GN=CAOG_007029 PE=3 SV=1
MM1 pKa = 7.03RR2 pKa = 11.84TVVPFILVAILVAVSVVDD20 pKa = 3.95SQPQPHH26 pKa = 6.28EE27 pKa = 4.19RR28 pKa = 11.84QRR30 pKa = 11.84LEE32 pKa = 3.62PVAYY36 pKa = 10.31RR37 pKa = 11.84FDD39 pKa = 4.19AEE41 pKa = 4.4HH42 pKa = 7.26DD43 pKa = 3.8GSEE46 pKa = 4.13LLEE49 pKa = 4.54GVDD52 pKa = 4.24IDD54 pKa = 5.6LDD56 pKa = 3.75TDD58 pKa = 3.66EE59 pKa = 5.98VDD61 pKa = 2.93MSRR64 pKa = 11.84FDD66 pKa = 3.83RR67 pKa = 11.84SSSSSSAAFDD77 pKa = 4.35LDD79 pKa = 3.97DD80 pKa = 5.92DD81 pKa = 4.55GEE83 pKa = 4.17EE84 pKa = 3.85MHH86 pKa = 6.67MDD88 pKa = 3.36IYY90 pKa = 11.16YY91 pKa = 10.76DD92 pKa = 3.7EE93 pKa = 5.41ANGVYY98 pKa = 10.23VDD100 pKa = 3.2AHH102 pKa = 6.16GNQLFTPAEE111 pKa = 4.13MEE113 pKa = 3.99ALQAQFDD120 pKa = 3.93NALEE124 pKa = 4.21NEE126 pKa = 4.23EE127 pKa = 4.05AAYY130 pKa = 9.92AYY132 pKa = 7.7HH133 pKa = 5.87TTTFEE138 pKa = 4.01GNAPIPSEE146 pKa = 3.8NVYY149 pKa = 10.69FEE151 pKa = 4.74ILDD154 pKa = 4.05PPEE157 pKa = 3.71IAYY160 pKa = 8.5MYY162 pKa = 9.91TGVISFNIGVDD173 pKa = 3.95FEE175 pKa = 5.13FMLDD179 pKa = 3.38PAYY182 pKa = 10.73LVLSEE187 pKa = 5.49PYY189 pKa = 9.97DD190 pKa = 3.53ACQPLNGEE198 pKa = 4.17QFHH201 pKa = 7.1DD202 pKa = 3.48AVVMVEE208 pKa = 4.69RR209 pKa = 11.84GTCSFIEE216 pKa = 4.07KK217 pKa = 10.0AINVASAGAAAMIVADD233 pKa = 4.5NEE235 pKa = 4.14PPEE238 pKa = 4.9DD239 pKa = 4.41SILVDD244 pKa = 3.54MVSDD248 pKa = 4.31DD249 pKa = 3.95GRR251 pKa = 11.84LASIPCVFIQTQDD264 pKa = 2.99GAKK267 pKa = 9.32IRR269 pKa = 11.84NAIYY273 pKa = 8.88QHH275 pKa = 6.07GVSGVPITIPLNITMQTQEE294 pKa = 4.15AFVDD298 pKa = 4.45SFVPWAPLLL307 pKa = 4.01

Molecular weight: 33.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D2W241|A0A0D2W241_CAPO3 Uncharacterized protein OS=Capsaspora owczarzaki (strain ATCC 30864) OX=595528 GN=CAOG_008372 PE=4 SV=1
MM1 pKa = 7.29SASASNIPRR10 pKa = 11.84TLNRR14 pKa = 11.84ISSTVSTILARR25 pKa = 11.84VAANSSTASSQPFKK39 pKa = 10.88LIPKK43 pKa = 9.24LPSAPRR49 pKa = 11.84TATARR54 pKa = 11.84RR55 pKa = 11.84GAWQPSPAQQAAQTKK70 pKa = 6.5QQKK73 pKa = 9.42

Molecular weight: 7.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8738

1056

9794

6129802

37

12732

625.9

67.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.821 ± 0.033

1.375 ± 0.019

5.412 ± 0.018

5.053 ± 0.022

3.538 ± 0.015

5.901 ± 0.021

2.524 ± 0.012

3.946 ± 0.016

3.684 ± 0.024

9.38 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.039 ± 0.009

3.518 ± 0.013

5.832 ± 0.025

4.745 ± 0.026

5.732 ± 0.019

9.548 ± 0.042

6.26 ± 0.023

6.615 ± 0.018

0.973 ± 0.006

2.103 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski