Amino acid dipepetide frequency for Idiomarina sp. MD25a

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.396AlaAla: 8.396 ± 0.131	0.89AlaCys: 0.89 ± 0.035	5.394AlaAsp: 5.394 ± 0.078	6.325AlaGlu: 6.325 ± 0.091	3.459AlaPhe: 3.459 ± 0.079	6.2AlaGly: 6.2 ± 0.087	1.964AlaHis: 1.964 ± 0.053	5.786AlaIle: 5.786 ± 0.097	4.306AlaLys: 4.306 ± 0.075	10.395AlaLeu: 10.395 ± 0.126	2.569AlaMet: 2.569 ± 0.059	3.266AlaAsn: 3.266 ± 0.069	3.171AlaPro: 3.171 ± 0.065	5.057AlaGln: 5.057 ± 0.092	4.704AlaArg: 4.704 ± 0.077	5.324AlaSer: 5.324 ± 0.084	4.426AlaThr: 4.426 ± 0.067	6.56AlaVal: 6.56 ± 0.08	1.099AlaTrp: 1.099 ± 0.041	2.372AlaTyr: 2.372 ± 0.063	0.0AlaXaa: 0.0 ± 0.0
Cys	0.75CysAla: 0.75 ± 0.033	0.137CysCys: 0.137 ± 0.014	0.57CysAsp: 0.57 ± 0.025	0.565CysGlu: 0.565 ± 0.024	0.358CysPhe: 0.358 ± 0.022	0.751CysGly: 0.751 ± 0.03	0.277CysHis: 0.277 ± 0.017	0.456CysIle: 0.456 ± 0.024	0.335CysLys: 0.335 ± 0.023	0.83CysLeu: 0.83 ± 0.032	0.172CysMet: 0.172 ± 0.015	0.266CysAsn: 0.266 ± 0.015	0.391CysPro: 0.391 ± 0.024	0.446CysGln: 0.446 ± 0.026	0.473CysArg: 0.473 ± 0.027	0.601CysSer: 0.601 ± 0.027	0.362CysThr: 0.362 ± 0.02	0.606CysVal: 0.606 ± 0.025	0.113CysTrp: 0.113 ± 0.013	0.295CysTyr: 0.295 ± 0.021	0.0CysXaa: 0.0 ± 0.0
Asp	5.369AspAla: 5.369 ± 0.083	0.488AspCys: 0.488 ± 0.027	3.84AspAsp: 3.84 ± 0.078	4.595AspGlu: 4.595 ± 0.099	2.476AspPhe: 2.476 ± 0.052	3.892AspGly: 3.892 ± 0.074	1.137AspHis: 1.137 ± 0.036	3.963AspIle: 3.963 ± 0.069	2.968AspLys: 2.968 ± 0.073	5.026AspLeu: 5.026 ± 0.082	1.411AspMet: 1.411 ± 0.045	2.45AspAsn: 2.45 ± 0.06	2.146AspPro: 2.146 ± 0.059	2.169AspGln: 2.169 ± 0.054	2.578AspArg: 2.578 ± 0.063	3.325AspSer: 3.325 ± 0.07	2.948AspThr: 2.948 ± 0.063	4.251AspVal: 4.251 ± 0.075	1.14AspTrp: 1.14 ± 0.04	2.284AspTyr: 2.284 ± 0.051	0.001AspXaa: 0.001 ± 0.001
Glu	5.723GluAla: 5.723 ± 0.098	0.457GluCys: 0.457 ± 0.021	2.991GluAsp: 2.991 ± 0.075	3.644GluGlu: 3.644 ± 0.089	2.437GluPhe: 2.437 ± 0.062	3.761GluGly: 3.761 ± 0.088	1.831GluHis: 1.831 ± 0.054	3.291GluIle: 3.291 ± 0.067	3.051GluLys: 3.051 ± 0.066	7.047GluLeu: 7.047 ± 0.103	1.514GluMet: 1.514 ± 0.05	2.302GluAsn: 2.302 ± 0.059	2.439GluPro: 2.439 ± 0.062	5.442GluGln: 5.442 ± 0.094	4.725GluArg: 4.725 ± 0.09	3.711GluSer: 3.711 ± 0.077	3.137GluThr: 3.137 ± 0.068	4.488GluVal: 4.488 ± 0.082	0.869GluTrp: 0.869 ± 0.034	1.83GluTyr: 1.83 ± 0.047	0.0GluXaa: 0.0 ± 0.0
Phe	3.754PheAla: 3.754 ± 0.075	0.388PheCys: 0.388 ± 0.022	2.863PheAsp: 2.863 ± 0.068	2.66PheGlu: 2.66 ± 0.062	1.606PhePhe: 1.606 ± 0.051	2.924PheGly: 2.924 ± 0.063	0.717PheHis: 0.717 ± 0.034	2.518PheIle: 2.518 ± 0.058	1.935PheLys: 1.935 ± 0.043	3.059PheLeu: 3.059 ± 0.069	0.884PheMet: 0.884 ± 0.033	1.911PheAsn: 1.911 ± 0.048	1.385PhePro: 1.385 ± 0.044	1.218PheGln: 1.218 ± 0.036	1.743PheArg: 1.743 ± 0.047	2.912PheSer: 2.912 ± 0.065	2.079PheThr: 2.079 ± 0.049	2.764PheVal: 2.764 ± 0.061	0.555PheTrp: 0.555 ± 0.027	1.333PheTyr: 1.333 ± 0.038	0.0PheXaa: 0.0 ± 0.0
Gly	5.566GlyAla: 5.566 ± 0.094	0.699GlyCys: 0.699 ± 0.031	3.935GlyAsp: 3.935 ± 0.079	4.522GlyGlu: 4.522 ± 0.085	3.232GlyPhe: 3.232 ± 0.069	4.863GlyGly: 4.863 ± 0.096	1.701GlyHis: 1.701 ± 0.044	4.399GlyIle: 4.399 ± 0.084	3.295GlyLys: 3.295 ± 0.076	6.744GlyLeu: 6.744 ± 0.105	1.9GlyMet: 1.9 ± 0.057	2.302GlyAsn: 2.302 ± 0.066	1.82GlyPro: 1.82 ± 0.048	3.026GlyGln: 3.026 ± 0.057	3.56GlyArg: 3.56 ± 0.073	4.036GlySer: 4.036 ± 0.072	3.393GlyThr: 3.393 ± 0.07	5.344GlyVal: 5.344 ± 0.086	1.027GlyTrp: 1.027 ± 0.034	2.522GlyTyr: 2.522 ± 0.065	0.001GlyXaa: 0.001 ± 0.001
His	1.898HisAla: 1.898 ± 0.056	0.34HisCys: 0.34 ± 0.022	1.28HisAsp: 1.28 ± 0.039	1.286HisGlu: 1.286 ± 0.039	1.017HisPhe: 1.017 ± 0.037	1.612HisGly: 1.612 ± 0.047	0.715HisHis: 0.715 ± 0.034	1.334HisIle: 1.334 ± 0.042	0.888HisLys: 0.888 ± 0.032	2.158HisLeu: 2.158 ± 0.045	0.517HisMet: 0.517 ± 0.024	0.788HisAsn: 0.788 ± 0.031	1.266HisPro: 1.266 ± 0.045	1.215HisGln: 1.215 ± 0.04	1.269HisArg: 1.269 ± 0.04	1.358HisSer: 1.358 ± 0.049	1.038HisThr: 1.038 ± 0.034	1.514HisVal: 1.514 ± 0.042	0.473HisTrp: 0.473 ± 0.023	0.902HisTyr: 0.902 ± 0.033	0.0HisXaa: 0.0 ± 0.0
Ile	6.211IleAla: 6.211 ± 0.085	0.533IleCys: 0.533 ± 0.029	4.286IleAsp: 4.286 ± 0.07	4.6IleGlu: 4.6 ± 0.08	1.916IlePhe: 1.916 ± 0.056	4.251IleGly: 4.251 ± 0.076	1.157IleHis: 1.157 ± 0.035	3.319IleIle: 3.319 ± 0.064	3.015IleLys: 3.015 ± 0.061	4.608IleLeu: 4.608 ± 0.082	1.11IleMet: 1.11 ± 0.036	2.594IleAsn: 2.594 ± 0.06	2.378IlePro: 2.378 ± 0.057	2.132IleGln: 2.132 ± 0.041	2.978IleArg: 2.978 ± 0.061	3.63IleSer: 3.63 ± 0.068	3.197IleThr: 3.197 ± 0.064	4.056IleVal: 4.056 ± 0.077	0.631IleTrp: 0.631 ± 0.027	1.532IleTyr: 1.532 ± 0.043	0.0IleXaa: 0.0 ± 0.0
Lys	4.398LysAla: 4.398 ± 0.068	0.23LysCys: 0.23 ± 0.019	2.389LysAsp: 2.389 ± 0.06	2.683LysGlu: 2.683 ± 0.072	1.321LysPhe: 1.321 ± 0.042	2.859LysGly: 2.859 ± 0.06	1.209LysHis: 1.209 ± 0.034	2.071LysIle: 2.071 ± 0.051	2.276LysLys: 2.276 ± 0.063	4.643LysLeu: 4.643 ± 0.085	1.017LysMet: 1.017 ± 0.038	1.629LysAsn: 1.629 ± 0.048	2.371LysPro: 2.371 ± 0.053	3.261LysGln: 3.261 ± 0.069	3.161LysArg: 3.161 ± 0.069	2.62LysSer: 2.62 ± 0.055	2.402LysThr: 2.402 ± 0.054	3.242LysVal: 3.242 ± 0.069	0.478LysTrp: 0.478 ± 0.023	1.099LysTyr: 1.099 ± 0.038	0.0LysXaa: 0.0 ± 0.0
Leu	10.365LeuAla: 10.365 ± 0.126	0.881LeuCys: 0.881 ± 0.033	5.707LeuAsp: 5.707 ± 0.093	6.018LeuGlu: 6.018 ± 0.095	3.88LeuPhe: 3.88 ± 0.077	6.799LeuGly: 6.799 ± 0.106	1.958LeuHis: 1.958 ± 0.051	5.994LeuIle: 5.994 ± 0.097	5.167LeuLys: 5.167 ± 0.087	10.585LeuLeu: 10.585 ± 0.156	2.697LeuMet: 2.697 ± 0.068	4.501LeuAsn: 4.501 ± 0.083	4.725LeuPro: 4.725 ± 0.083	4.166LeuGln: 4.166 ± 0.089	5.089LeuArg: 5.089 ± 0.082	7.4LeuSer: 7.4 ± 0.114	6.283LeuThr: 6.283 ± 0.089	7.019LeuVal: 7.019 ± 0.108	1.164LeuTrp: 1.164 ± 0.04	2.537LeuTyr: 2.537 ± 0.056	0.0LeuXaa: 0.0 ± 0.0
Met	2.456MetAla: 2.456 ± 0.051	0.157MetCys: 0.157 ± 0.015	1.181MetAsp: 1.181 ± 0.039	1.13MetGlu: 1.13 ± 0.041	0.781MetPhe: 0.781 ± 0.034	1.718MetGly: 1.718 ± 0.049	0.476MetHis: 0.476 ± 0.027	1.248MetIle: 1.248 ± 0.037	1.242MetLys: 1.242 ± 0.037	2.564MetLeu: 2.564 ± 0.068	0.678MetMet: 0.678 ± 0.031	1.044MetAsn: 1.044 ± 0.033	1.227MetPro: 1.227 ± 0.04	1.267MetGln: 1.267 ± 0.042	1.327MetArg: 1.327 ± 0.04	1.911MetSer: 1.911 ± 0.049	1.611MetThr: 1.611 ± 0.046	1.67MetVal: 1.67 ± 0.043	0.223MetTrp: 0.223 ± 0.017	0.503MetTyr: 0.503 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	3.494AsnAla: 3.494 ± 0.07	0.308AsnCys: 0.308 ± 0.02	2.531AsnAsp: 2.531 ± 0.062	2.588AsnGlu: 2.588 ± 0.063	1.347AsnPhe: 1.347 ± 0.04	2.701AsnGly: 2.701 ± 0.058	0.839AsnHis: 0.839 ± 0.031	2.167AsnIle: 2.167 ± 0.056	1.624AsnLys: 1.624 ± 0.047	3.372AsnLeu: 3.372 ± 0.072	0.825AsnMet: 0.825 ± 0.033	1.596AsnAsn: 1.596 ± 0.062	1.96AsnPro: 1.96 ± 0.052	2.197AsnGln: 2.197 ± 0.062	2.071AsnArg: 2.071 ± 0.052	2.2AsnSer: 2.2 ± 0.067	2.029AsnThr: 2.029 ± 0.051	2.578AsnVal: 2.578 ± 0.064	0.618AsnTrp: 0.618 ± 0.027	1.304AsnTyr: 1.304 ± 0.042	0.0AsnXaa: 0.0 ± 0.0
Pro	3.236ProAla: 3.236 ± 0.062	0.296ProCys: 0.296 ± 0.018	2.582ProAsp: 2.582 ± 0.051	3.398ProGlu: 3.398 ± 0.08	1.653ProPhe: 1.653 ± 0.042	2.596ProGly: 2.596 ± 0.061	0.923ProHis: 0.923 ± 0.03	2.434ProIle: 2.434 ± 0.057	1.837ProLys: 1.837 ± 0.049	4.238ProLeu: 4.238 ± 0.08	1.077ProMet: 1.077 ± 0.033	1.788ProAsn: 1.788 ± 0.046	1.251ProPro: 1.251 ± 0.043	1.935ProGln: 1.935 ± 0.059	1.616ProArg: 1.616 ± 0.049	2.449ProSer: 2.449 ± 0.048	2.206ProThr: 2.206 ± 0.047	3.088ProVal: 3.088 ± 0.064	0.53ProTrp: 0.53 ± 0.024	1.243ProTyr: 1.243 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	5.062GlnAla: 5.062 ± 0.071	0.396GlnCys: 0.396 ± 0.025	2.064GlnAsp: 2.064 ± 0.052	2.317GlnGlu: 2.317 ± 0.052	1.984GlnPhe: 1.984 ± 0.053	3.322GlnGly: 3.322 ± 0.061	1.453GlnHis: 1.453 ± 0.05	2.32GlnIle: 2.32 ± 0.053	1.944GlnLys: 1.944 ± 0.047	6.637GlnLeu: 6.637 ± 0.107	1.173GlnMet: 1.173 ± 0.04	1.492GlnAsn: 1.492 ± 0.046	2.358GlnPro: 2.358 ± 0.064	4.637GlnGln: 4.637 ± 0.115	3.773GlnArg: 3.773 ± 0.085	3.167GlnSer: 3.167 ± 0.065	2.525GlnThr: 2.525 ± 0.061	3.699GlnVal: 3.699 ± 0.071	0.974GlnTrp: 0.974 ± 0.041	1.302GlnTyr: 1.302 ± 0.036	0.0GlnXaa: 0.0 ± 0.0
Arg	4.501ArgAla: 4.501 ± 0.083	0.462ArgCys: 0.462 ± 0.021	3.076ArgAsp: 3.076 ± 0.057	3.512ArgGlu: 3.512 ± 0.064	2.503ArgPhe: 2.503 ± 0.056	3.153ArgGly: 3.153 ± 0.056	1.332ArgHis: 1.332 ± 0.045	3.389ArgIle: 3.389 ± 0.067	2.33ArgLys: 2.33 ± 0.063	6.088ArgLeu: 6.088 ± 0.105	1.43ArgMet: 1.43 ± 0.039	1.993ArgAsn: 1.993 ± 0.044	1.972ArgPro: 1.972 ± 0.05	2.753ArgGln: 2.753 ± 0.071	2.985ArgArg: 2.985 ± 0.069	3.107ArgSer: 3.107 ± 0.052	2.38ArgThr: 2.38 ± 0.051	4.136ArgVal: 4.136 ± 0.066	0.966ArgTrp: 0.966 ± 0.037	2.084ArgTyr: 2.084 ± 0.054	0.0ArgXaa: 0.0 ± 0.0
Ser	5.741SerAla: 5.741 ± 0.09	0.537SerCys: 0.537 ± 0.026	3.838SerAsp: 3.838 ± 0.065	4.226SerGlu: 4.226 ± 0.07	2.527SerPhe: 2.527 ± 0.064	4.691SerGly: 4.691 ± 0.085	1.417SerHis: 1.417 ± 0.049	3.584SerIle: 3.584 ± 0.069	2.475SerLys: 2.475 ± 0.053	6.54SerLeu: 6.54 ± 0.096	1.558SerMet: 1.558 ± 0.045	2.335SerAsn: 2.335 ± 0.054	2.358SerPro: 2.358 ± 0.053	3.117SerGln: 3.117 ± 0.073	3.152SerArg: 3.152 ± 0.058	3.971SerSer: 3.971 ± 0.077	3.011SerThr: 3.011 ± 0.069	4.719SerVal: 4.719 ± 0.076	0.794SerTrp: 0.794 ± 0.031	1.851SerTyr: 1.851 ± 0.048	0.0SerXaa: 0.0 ± 0.0
Thr	4.698ThrAla: 4.698 ± 0.074	0.412ThrCys: 0.412 ± 0.022	3.123ThrAsp: 3.123 ± 0.059	3.507ThrGlu: 3.507 ± 0.077	1.956ThrPhe: 1.956 ± 0.049	3.909ThrGly: 3.909 ± 0.068	1.214ThrHis: 1.214 ± 0.036	3.074ThrIle: 3.074 ± 0.062	1.636ThrLys: 1.636 ± 0.047	6.067ThrLeu: 6.067 ± 0.08	1.013ThrMet: 1.013 ± 0.033	1.618ThrAsn: 1.618 ± 0.046	2.525ThrPro: 2.525 ± 0.063	2.837ThrGln: 2.837 ± 0.059	2.587ThrArg: 2.587 ± 0.057	3.05ThrSer: 3.05 ± 0.072	2.767ThrThr: 2.767 ± 0.059	3.9ThrVal: 3.9 ± 0.079	0.639ThrTrp: 0.639 ± 0.027	1.515ThrTyr: 1.515 ± 0.036	0.0ThrXaa: 0.0 ± 0.0
Val	6.804ValAla: 6.804 ± 0.095	0.671ValCys: 0.671 ± 0.031	4.501ValAsp: 4.501 ± 0.088	4.921ValGlu: 4.921 ± 0.084	2.741ValPhe: 2.741 ± 0.062	4.932ValGly: 4.932 ± 0.088	1.341ValHis: 1.341 ± 0.035	4.6ValIle: 4.6 ± 0.085	3.442ValLys: 3.442 ± 0.069	6.909ValLeu: 6.909 ± 0.09	1.934ValMet: 1.934 ± 0.048	3.05ValAsn: 3.05 ± 0.063	2.768ValPro: 2.768 ± 0.06	2.505ValGln: 2.505 ± 0.061	3.508ValArg: 3.508 ± 0.068	4.983ValSer: 4.983 ± 0.084	4.251ValThr: 4.251 ± 0.07	5.458ValVal: 5.458 ± 0.086	0.821ValTrp: 0.821 ± 0.037	1.875ValTyr: 1.875 ± 0.051	0.0ValXaa: 0.0 ± 0.0
Trp	0.858TrpAla: 0.858 ± 0.032	0.14TrpCys: 0.14 ± 0.015	0.588TrpAsp: 0.588 ± 0.025	0.493TrpGlu: 0.493 ± 0.025	0.683TrpPhe: 0.683 ± 0.032	0.822TrpGly: 0.822 ± 0.035	0.424TrpHis: 0.424 ± 0.022	0.678TrpIle: 0.678 ± 0.026	0.403TrpLys: 0.403 ± 0.024	2.236TrpLeu: 2.236 ± 0.067	0.377TrpMet: 0.377 ± 0.022	0.416TrpAsn: 0.416 ± 0.021	0.543TrpPro: 0.543 ± 0.027	1.235TrpGln: 1.235 ± 0.04	0.918TrpArg: 0.918 ± 0.034	0.795TrpSer: 0.795 ± 0.033	0.565TrpThr: 0.565 ± 0.028	1.017TrpVal: 1.017 ± 0.04	0.247TrpTrp: 0.247 ± 0.018	0.406TrpTyr: 0.406 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.385TyrAla: 2.385 ± 0.056	0.331TyrCys: 0.331 ± 0.019	1.719TyrAsp: 1.719 ± 0.051	1.701TyrGlu: 1.701 ± 0.047	1.332TyrPhe: 1.332 ± 0.043	2.131TyrGly: 2.131 ± 0.057	0.785TyrHis: 0.785 ± 0.036	1.507TyrIle: 1.507 ± 0.043	1.19TyrLys: 1.19 ± 0.039	3.189TyrLeu: 3.189 ± 0.059	0.554TyrMet: 0.554 ± 0.028	1.008TyrAsn: 1.008 ± 0.038	1.322TyrPro: 1.322 ± 0.047	1.984TyrGln: 1.984 ± 0.056	1.935TyrArg: 1.935 ± 0.048	1.89TyrSer: 1.89 ± 0.048	1.436TyrThr: 1.436 ± 0.048	1.906TyrVal: 1.906 ± 0.048	0.483TyrTrp: 0.483 ± 0.025	1.013TyrTyr: 1.013 ± 0.038	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 2463 proteins (833508 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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