Amino acid dipepetide frequency for Bacillus phage Nf (Bacteriophage Nf)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.867AlaAla: 2.867 ± 0.627
0.675AlaCys: 0.675 ± 0.344
2.699AlaAsp: 2.699 ± 0.599
3.373AlaGlu: 3.373 ± 0.901
2.699AlaPhe: 2.699 ± 1.141
3.879AlaGly: 3.879 ± 0.817
0.506AlaHis: 0.506 ± 0.294
4.385AlaIle: 4.385 ± 1.329
4.048AlaLys: 4.048 ± 0.838
3.373AlaLeu: 3.373 ± 1.016
1.349AlaMet: 1.349 ± 0.506
4.217AlaAsn: 4.217 ± 0.896
1.012AlaPro: 1.012 ± 0.338
2.867AlaGln: 2.867 ± 0.932
2.193AlaArg: 2.193 ± 0.599
5.06AlaSer: 5.06 ± 1.317
4.723AlaThr: 4.723 ± 0.996
4.048AlaVal: 4.048 ± 0.774
0.843AlaTrp: 0.843 ± 0.371
3.036AlaTyr: 3.036 ± 0.451
0.0AlaXaa: 0.0 ± 0.0
Cys
0.337CysAla: 0.337 ± 0.216
0.0CysCys: 0.0 ± 0.0
0.843CysAsp: 0.843 ± 0.453
0.843CysGlu: 0.843 ± 0.382
0.337CysPhe: 0.337 ± 0.194
0.675CysGly: 0.675 ± 0.368
0.169CysHis: 0.169 ± 0.155
0.675CysIle: 0.675 ± 0.31
0.0CysLys: 0.0 ± 0.0
0.337CysLeu: 0.337 ± 0.224
0.169CysMet: 0.169 ± 0.183
1.012CysAsn: 1.012 ± 0.486
0.0CysPro: 0.0 ± 0.0
0.506CysGln: 0.506 ± 0.331
0.169CysArg: 0.169 ± 0.145
0.843CysSer: 0.843 ± 0.343
0.337CysThr: 0.337 ± 0.227
0.843CysVal: 0.843 ± 0.372
0.0CysTrp: 0.0 ± 0.0
0.675CysTyr: 0.675 ± 0.259
0.0CysXaa: 0.0 ± 0.0
Asp
1.687AspAla: 1.687 ± 0.489
0.843AspCys: 0.843 ± 0.277
4.048AspAsp: 4.048 ± 0.678
5.397AspGlu: 5.397 ± 0.741
2.867AspPhe: 2.867 ± 0.76
5.566AspGly: 5.566 ± 1.313
0.337AspHis: 0.337 ± 0.246
5.06AspIle: 5.06 ± 0.96
3.036AspLys: 3.036 ± 0.863
4.385AspLeu: 4.385 ± 1.252
1.012AspMet: 1.012 ± 0.464
3.711AspAsn: 3.711 ± 0.683
1.855AspPro: 1.855 ± 0.43
0.337AspGln: 0.337 ± 0.257
2.361AspArg: 2.361 ± 0.53
4.385AspSer: 4.385 ± 0.671
4.217AspThr: 4.217 ± 0.974
5.229AspVal: 5.229 ± 0.837
1.012AspTrp: 1.012 ± 0.542
3.036AspTyr: 3.036 ± 0.975
0.0AspXaa: 0.0 ± 0.0
Glu
2.53GluAla: 2.53 ± 0.776
0.506GluCys: 0.506 ± 0.256
3.711GluAsp: 3.711 ± 1.036
4.217GluGlu: 4.217 ± 0.869
2.699GluPhe: 2.699 ± 0.762
4.385GluGly: 4.385 ± 0.678
2.193GluHis: 2.193 ± 0.754
7.084GluIle: 7.084 ± 1.094
6.241GluLys: 6.241 ± 1.156
4.723GluLeu: 4.723 ± 1.131
1.687GluMet: 1.687 ± 0.563
3.373GluAsn: 3.373 ± 0.629
0.843GluPro: 0.843 ± 0.34
2.361GluGln: 2.361 ± 0.589
3.879GluArg: 3.879 ± 0.761
4.385GluSer: 4.385 ± 0.831
3.711GluThr: 3.711 ± 0.912
4.385GluVal: 4.385 ± 1.28
1.181GluTrp: 1.181 ± 0.475
4.723GluTyr: 4.723 ± 0.646
0.0GluXaa: 0.0 ± 0.0
Phe
2.024PheAla: 2.024 ± 0.408
0.0PheCys: 0.0 ± 0.0
3.373PheAsp: 3.373 ± 0.846
3.711PheGlu: 3.711 ± 0.702
1.518PhePhe: 1.518 ± 0.428
2.193PheGly: 2.193 ± 0.631
1.349PheHis: 1.349 ± 0.507
3.542PheIle: 3.542 ± 1.202
4.217PheLys: 4.217 ± 1.156
2.193PheLeu: 2.193 ± 0.578
1.012PheMet: 1.012 ± 0.308
2.867PheAsn: 2.867 ± 0.716
0.843PhePro: 0.843 ± 0.414
1.012PheGln: 1.012 ± 0.321
1.518PheArg: 1.518 ± 0.472
2.53PheSer: 2.53 ± 0.662
2.53PheThr: 2.53 ± 0.578
2.024PheVal: 2.024 ± 0.497
0.169PheTrp: 0.169 ± 0.171
1.349PheTyr: 1.349 ± 0.547
0.0PheXaa: 0.0 ± 0.0
Gly
3.711GlyAla: 3.711 ± 0.745
0.506GlyCys: 0.506 ± 0.273
3.879GlyAsp: 3.879 ± 0.969
3.373GlyGlu: 3.373 ± 0.674
2.699GlyPhe: 2.699 ± 0.768
3.711GlyGly: 3.711 ± 1.299
1.181GlyHis: 1.181 ± 0.372
4.554GlyIle: 4.554 ± 1.289
4.385GlyLys: 4.385 ± 1.005
5.06GlyLeu: 5.06 ± 0.674
1.518GlyMet: 1.518 ± 0.521
6.578GlyAsn: 6.578 ± 1.055
0.0GlyPro: 0.0 ± 0.0
2.024GlyGln: 2.024 ± 0.63
1.518GlyArg: 1.518 ± 0.533
5.566GlySer: 5.566 ± 1.402
3.373GlyThr: 3.373 ± 0.661
6.241GlyVal: 6.241 ± 1.574
0.675GlyTrp: 0.675 ± 0.579
4.048GlyTyr: 4.048 ± 0.742
0.0GlyXaa: 0.0 ± 0.0
His
1.518HisAla: 1.518 ± 0.592
0.0HisCys: 0.0 ± 0.0
0.337HisAsp: 0.337 ± 0.21
1.687HisGlu: 1.687 ± 0.65
0.169HisPhe: 0.169 ± 0.18
0.675HisGly: 0.675 ± 0.346
0.506HisHis: 0.506 ± 0.327
1.012HisIle: 1.012 ± 0.359
0.843HisLys: 0.843 ± 0.384
1.012HisLeu: 1.012 ± 0.486
0.337HisMet: 0.337 ± 0.242
0.675HisAsn: 0.675 ± 0.394
0.506HisPro: 0.506 ± 0.321
0.843HisGln: 0.843 ± 0.324
0.337HisArg: 0.337 ± 0.221
1.181HisSer: 1.181 ± 0.52
0.843HisThr: 0.843 ± 0.338
1.349HisVal: 1.349 ± 0.375
0.337HisTrp: 0.337 ± 0.272
1.349HisTyr: 1.349 ± 0.412
0.0HisXaa: 0.0 ± 0.0
Ile
4.217IleAla: 4.217 ± 0.52
0.675IleCys: 0.675 ± 0.24
5.903IleAsp: 5.903 ± 0.833
4.723IleGlu: 4.723 ± 1.153
2.193IlePhe: 2.193 ± 0.691
5.06IleGly: 5.06 ± 1.289
1.518IleHis: 1.518 ± 0.387
4.385IleIle: 4.385 ± 0.75
6.241IleLys: 6.241 ± 1.106
4.385IleLeu: 4.385 ± 0.637
0.843IleMet: 0.843 ± 0.352
5.06IleAsn: 5.06 ± 0.791
1.855IlePro: 1.855 ± 0.586
2.699IleGln: 2.699 ± 0.955
2.867IleArg: 2.867 ± 0.522
5.229IleSer: 5.229 ± 1.448
3.711IleThr: 3.711 ± 0.664
4.723IleVal: 4.723 ± 0.653
0.169IleTrp: 0.169 ± 0.18
3.205IleTyr: 3.205 ± 0.64
0.0IleXaa: 0.0 ± 0.0
Lys
5.229LysAla: 5.229 ± 1.05
0.675LysCys: 0.675 ± 0.291
3.542LysAsp: 3.542 ± 1.01
6.409LysGlu: 6.409 ± 1.122
2.867LysPhe: 2.867 ± 0.671
3.373LysGly: 3.373 ± 0.626
1.349LysHis: 1.349 ± 0.505
4.385LysIle: 4.385 ± 0.893
5.229LysLys: 5.229 ± 1.32
6.578LysLeu: 6.578 ± 1.645
4.217LysMet: 4.217 ± 0.681
5.735LysAsn: 5.735 ± 1.015
2.699LysPro: 2.699 ± 0.513
2.867LysGln: 2.867 ± 0.774
3.205LysArg: 3.205 ± 0.732
4.385LysSer: 4.385 ± 0.804
5.735LysThr: 5.735 ± 0.803
4.385LysVal: 4.385 ± 0.637
1.181LysTrp: 1.181 ± 0.389
2.53LysTyr: 2.53 ± 0.843
0.0LysXaa: 0.0 ± 0.0
Leu
3.879LeuAla: 3.879 ± 0.909
0.843LeuCys: 0.843 ± 0.477
4.385LeuAsp: 4.385 ± 0.888
5.903LeuGlu: 5.903 ± 1.121
2.867LeuPhe: 2.867 ± 0.898
3.373LeuGly: 3.373 ± 0.72
1.012LeuHis: 1.012 ± 0.348
3.879LeuIle: 3.879 ± 0.692
7.421LeuLys: 7.421 ± 1.204
5.735LeuLeu: 5.735 ± 1.603
2.867LeuMet: 2.867 ± 0.619
6.578LeuAsn: 6.578 ± 0.797
3.036LeuPro: 3.036 ± 0.889
1.687LeuGln: 1.687 ± 0.748
3.879LeuArg: 3.879 ± 0.99
7.084LeuSer: 7.084 ± 0.964
3.879LeuThr: 3.879 ± 0.656
4.723LeuVal: 4.723 ± 0.914
0.506LeuTrp: 0.506 ± 0.283
4.217LeuTyr: 4.217 ± 0.946
0.0LeuXaa: 0.0 ± 0.0
Met
1.181MetAla: 1.181 ± 0.404
0.169MetCys: 0.169 ± 0.18
1.012MetAsp: 1.012 ± 0.387
1.518MetGlu: 1.518 ± 0.54
1.687MetPhe: 1.687 ± 0.633
1.855MetGly: 1.855 ± 0.438
0.337MetHis: 0.337 ± 0.22
2.193MetIle: 2.193 ± 0.658
2.193MetLys: 2.193 ± 0.706
1.855MetLeu: 1.855 ± 0.495
0.506MetMet: 0.506 ± 0.262
1.012MetAsn: 1.012 ± 0.396
0.843MetPro: 0.843 ± 0.334
1.181MetGln: 1.181 ± 0.256
2.024MetArg: 2.024 ± 0.671
1.518MetSer: 1.518 ± 0.364
2.53MetThr: 2.53 ± 0.705
2.193MetVal: 2.193 ± 0.571
0.337MetTrp: 0.337 ± 0.246
1.181MetTyr: 1.181 ± 0.364
0.0MetXaa: 0.0 ± 0.0
Asn
5.397AsnAla: 5.397 ± 1.356
1.181AsnCys: 1.181 ± 0.946
4.554AsnAsp: 4.554 ± 0.964
4.385AsnGlu: 4.385 ± 0.986
2.53AsnPhe: 2.53 ± 0.671
5.06AsnGly: 5.06 ± 1.629
0.169AsnHis: 0.169 ± 0.148
3.711AsnIle: 3.711 ± 1.064
5.566AsnLys: 5.566 ± 1.118
5.397AsnLeu: 5.397 ± 0.976
1.687AsnMet: 1.687 ± 0.474
5.397AsnAsn: 5.397 ± 1.091
3.879AsnPro: 3.879 ± 0.696
2.361AsnGln: 2.361 ± 0.736
1.855AsnArg: 1.855 ± 0.468
3.879AsnSer: 3.879 ± 0.727
5.903AsnThr: 5.903 ± 1.326
4.723AsnVal: 4.723 ± 0.827
0.675AsnTrp: 0.675 ± 0.225
2.361AsnTyr: 2.361 ± 0.591
0.0AsnXaa: 0.0 ± 0.0
Pro
2.193ProAla: 2.193 ± 0.83
0.337ProCys: 0.337 ± 0.227
2.024ProAsp: 2.024 ± 0.65
1.855ProGlu: 1.855 ± 0.585
1.181ProPhe: 1.181 ± 0.543
0.169ProGly: 0.169 ± 0.18
0.506ProHis: 0.506 ± 0.287
0.843ProIle: 0.843 ± 0.269
1.855ProLys: 1.855 ± 0.48
3.373ProLeu: 3.373 ± 0.625
1.012ProMet: 1.012 ± 0.491
2.024ProAsn: 2.024 ± 0.481
0.675ProPro: 0.675 ± 0.394
0.675ProGln: 0.675 ± 0.339
1.181ProArg: 1.181 ± 0.374
2.53ProSer: 2.53 ± 0.792
0.843ProThr: 0.843 ± 0.293
2.53ProVal: 2.53 ± 0.69
0.0ProTrp: 0.0 ± 0.0
2.024ProTyr: 2.024 ± 0.412
0.0ProXaa: 0.0 ± 0.0
Gln
2.024GlnAla: 2.024 ± 0.439
0.0GlnCys: 0.0 ± 0.0
0.675GlnAsp: 0.675 ± 0.287
2.361GlnGlu: 2.361 ± 0.596
1.687GlnPhe: 1.687 ± 0.432
2.024GlnGly: 2.024 ± 0.453
0.337GlnHis: 0.337 ± 0.194
2.699GlnIle: 2.699 ± 0.822
2.53GlnLys: 2.53 ± 0.573
3.711GlnLeu: 3.711 ± 0.931
1.181GlnMet: 1.181 ± 0.433
1.855GlnAsn: 1.855 ± 0.604
0.843GlnPro: 0.843 ± 0.451
1.518GlnGln: 1.518 ± 0.9
1.687GlnArg: 1.687 ± 0.661
1.181GlnSer: 1.181 ± 0.394
1.687GlnThr: 1.687 ± 0.559
2.361GlnVal: 2.361 ± 0.455
0.675GlnTrp: 0.675 ± 0.431
1.518GlnTyr: 1.518 ± 0.583
0.0GlnXaa: 0.0 ± 0.0
Arg
1.855ArgAla: 1.855 ± 0.636
0.169ArgCys: 0.169 ± 0.148
2.361ArgAsp: 2.361 ± 0.531
2.867ArgGlu: 2.867 ± 0.688
2.024ArgPhe: 2.024 ± 0.619
3.205ArgGly: 3.205 ± 0.594
0.337ArgHis: 0.337 ± 0.31
3.542ArgIle: 3.542 ± 0.514
3.036ArgLys: 3.036 ± 0.738
2.699ArgLeu: 2.699 ± 0.866
1.855ArgMet: 1.855 ± 0.66
3.205ArgAsn: 3.205 ± 0.699
1.518ArgPro: 1.518 ± 0.286
1.012ArgGln: 1.012 ± 0.406
1.181ArgArg: 1.181 ± 0.408
1.687ArgSer: 1.687 ± 0.61
3.542ArgThr: 3.542 ± 0.808
1.855ArgVal: 1.855 ± 0.388
0.675ArgTrp: 0.675 ± 0.302
1.012ArgTyr: 1.012 ± 0.356
0.0ArgXaa: 0.0 ± 0.0
Ser
5.06SerAla: 5.06 ± 1.337
0.169SerCys: 0.169 ± 0.145
5.06SerAsp: 5.06 ± 0.864
4.554SerGlu: 4.554 ± 0.966
2.53SerPhe: 2.53 ± 0.918
5.06SerGly: 5.06 ± 1.222
0.337SerHis: 0.337 ± 0.252
3.542SerIle: 3.542 ± 0.84
4.554SerLys: 4.554 ± 0.946
6.578SerLeu: 6.578 ± 1.015
1.349SerMet: 1.349 ± 0.437
5.229SerAsn: 5.229 ± 1.087
1.349SerPro: 1.349 ± 0.434
1.687SerGln: 1.687 ± 0.571
3.036SerArg: 3.036 ± 0.928
4.723SerSer: 4.723 ± 0.681
4.048SerThr: 4.048 ± 0.719
3.879SerVal: 3.879 ± 1.094
0.506SerTrp: 0.506 ± 0.276
4.385SerTyr: 4.385 ± 0.793
0.0SerXaa: 0.0 ± 0.0
Thr
4.891ThrAla: 4.891 ± 0.909
0.506ThrCys: 0.506 ± 0.28
3.711ThrAsp: 3.711 ± 0.731
3.373ThrGlu: 3.373 ± 0.845
2.024ThrPhe: 2.024 ± 0.513
5.735ThrGly: 5.735 ± 1.068
0.843ThrHis: 0.843 ± 0.394
6.241ThrIle: 6.241 ± 1.16
4.723ThrLys: 4.723 ± 1.254
5.566ThrLeu: 5.566 ± 1.073
1.181ThrMet: 1.181 ± 0.461
3.373ThrAsn: 3.373 ± 0.813
2.361ThrPro: 2.361 ± 0.473
2.361ThrGln: 2.361 ± 0.64
2.193ThrArg: 2.193 ± 0.586
3.879ThrSer: 3.879 ± 0.784
6.409ThrThr: 6.409 ± 1.903
4.217ThrVal: 4.217 ± 0.745
1.181ThrTrp: 1.181 ± 0.265
2.193ThrTyr: 2.193 ± 0.433
0.0ThrXaa: 0.0 ± 0.0
Val
4.048ValAla: 4.048 ± 1.05
1.012ValCys: 1.012 ± 0.388
4.723ValAsp: 4.723 ± 1.425
4.554ValGlu: 4.554 ± 0.793
2.699ValPhe: 2.699 ± 0.918
4.217ValGly: 4.217 ± 0.707
1.181ValHis: 1.181 ± 0.438
3.879ValIle: 3.879 ± 0.651
6.747ValLys: 6.747 ± 0.944
4.217ValLeu: 4.217 ± 1.017
1.687ValMet: 1.687 ± 0.425
4.554ValAsn: 4.554 ± 0.927
1.855ValPro: 1.855 ± 0.431
2.193ValGln: 2.193 ± 0.38
2.361ValArg: 2.361 ± 0.672
4.891ValSer: 4.891 ± 1.111
6.072ValThr: 6.072 ± 0.938
3.205ValVal: 3.205 ± 0.996
1.012ValTrp: 1.012 ± 0.438
2.867ValTyr: 2.867 ± 0.591
0.0ValXaa: 0.0 ± 0.0
Trp
1.012TrpAla: 1.012 ± 0.494
0.0TrpCys: 0.0 ± 0.0
0.169TrpAsp: 0.169 ± 0.158
0.675TrpGlu: 0.675 ± 0.271
1.012TrpPhe: 1.012 ± 0.405
0.169TrpGly: 0.169 ± 0.161
0.337TrpHis: 0.337 ± 0.238
0.337TrpIle: 0.337 ± 0.19
0.675TrpLys: 0.675 ± 0.327
1.518TrpLeu: 1.518 ± 0.572
0.337TrpMet: 0.337 ± 0.213
1.181TrpAsn: 1.181 ± 0.469
0.0TrpPro: 0.0 ± 0.0
0.843TrpGln: 0.843 ± 0.315
0.169TrpArg: 0.169 ± 0.18
0.843TrpSer: 0.843 ± 0.289
1.012TrpThr: 1.012 ± 0.587
1.181TrpVal: 1.181 ± 0.643
0.0TrpTrp: 0.0 ± 0.0
0.337TrpTyr: 0.337 ± 0.184
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.193TyrAla: 2.193 ± 0.723
0.506TyrCys: 0.506 ± 0.322
3.373TyrAsp: 3.373 ± 0.833
3.036TyrGlu: 3.036 ± 0.498
1.687TyrPhe: 1.687 ± 0.49
4.385TyrGly: 4.385 ± 1.035
1.012TyrHis: 1.012 ± 0.434
3.711TyrIle: 3.711 ± 0.701
3.036TyrLys: 3.036 ± 0.75
5.06TyrLeu: 5.06 ± 0.938
1.349TyrMet: 1.349 ± 0.451
3.036TyrAsn: 3.036 ± 0.842
1.855TyrPro: 1.855 ± 0.503
1.518TyrGln: 1.518 ± 0.644
2.024TyrArg: 2.024 ± 0.694
1.855TyrSer: 1.855 ± 0.545
1.855TyrThr: 1.855 ± 0.49
3.879TyrVal: 3.879 ± 0.962
0.675TyrTrp: 0.675 ± 0.336
1.687TyrTyr: 1.687 ± 0.506
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 27 proteins (5930 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski