Candidatus Methanohalarchaeum thermophilum

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Methanonatronarchaeia; Methanonatronarchaeales; Methanonatronarchaeaceae; Candidatus Methanohalarchaeum

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2152 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q6DXN8|A0A1Q6DXN8_9EURY Geranylgeranyl reductase flavoprotein OS=Candidatus Methanohalarchaeum thermophilum OX=1903181 GN=BTN85_1641 PE=4 SV=1
MM1 pKa = 7.8AYY3 pKa = 9.62KK4 pKa = 10.4KK5 pKa = 10.57ALLSLMVIGLVGVAAGAGTFAFFTDD30 pKa = 3.7EE31 pKa = 4.37EE32 pKa = 4.77KK33 pKa = 10.69ATGNTLTAGTIDD45 pKa = 4.43LAVNGQNPWNEE56 pKa = 4.0TFTIDD61 pKa = 5.47DD62 pKa = 4.18MKK64 pKa = 10.73PCEE67 pKa = 3.86KK68 pKa = 9.92TGYY71 pKa = 9.65IVANITNEE79 pKa = 3.57GSNPAKK85 pKa = 9.55VYY87 pKa = 11.25KK88 pKa = 9.47MINVTSCDD96 pKa = 3.39TGLEE100 pKa = 4.09IYY102 pKa = 7.61EE103 pKa = 4.36TTDD106 pKa = 2.93GEE108 pKa = 4.76MYY110 pKa = 10.53SSEE113 pKa = 4.62PEE115 pKa = 3.62WQAEE119 pKa = 3.77KK120 pKa = 10.7DD121 pKa = 3.64GRR123 pKa = 11.84VDD125 pKa = 5.3DD126 pKa = 3.69IHH128 pKa = 6.6TVTDD132 pKa = 3.51YY133 pKa = 12.11SMMIEE138 pKa = 4.33LYY140 pKa = 10.39EE141 pKa = 4.46NEE143 pKa = 4.27TTFYY147 pKa = 11.35ADD149 pKa = 4.37DD150 pKa = 4.97PYY152 pKa = 11.74ADD154 pKa = 4.31SEE156 pKa = 4.44AFSIGDD162 pKa = 3.73DD163 pKa = 3.54VTVGDD168 pKa = 5.62KK169 pKa = 10.9EE170 pKa = 4.53GTWFYY175 pKa = 11.02LGEE178 pKa = 4.15LQPGEE183 pKa = 4.27VMKK186 pKa = 9.91VTQDD190 pKa = 3.31YY191 pKa = 11.22HH192 pKa = 8.25LLNDD196 pKa = 4.19TGNEE200 pKa = 4.05YY201 pKa = 10.73QGDD204 pKa = 4.13AFTFDD209 pKa = 3.13ITFGATQLNDD219 pKa = 3.16DD220 pKa = 4.0TTIQGTVEE228 pKa = 4.11DD229 pKa = 4.41VSVSSAEE236 pKa = 4.03EE237 pKa = 3.67

Molecular weight: 25.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q6DWA9|A0A1Q6DWA9_9EURY IS605 OrfB-like transposable element containing RNAse H-like and Zn finger domain OS=Candidatus Methanohalarchaeum thermophilum OX=1903181 GN=BTN85_1146 PE=4 SV=1
MM1 pKa = 7.54KK2 pKa = 9.47MPRR5 pKa = 11.84RR6 pKa = 11.84IRR8 pKa = 11.84TYY10 pKa = 9.91CPYY13 pKa = 10.47CNEE16 pKa = 4.2HH17 pKa = 6.45NEE19 pKa = 4.2HH20 pKa = 6.07EE21 pKa = 4.48VEE23 pKa = 4.27KK24 pKa = 11.07VRR26 pKa = 11.84NNPPSEE32 pKa = 4.29MKK34 pKa = 9.49WGQRR38 pKa = 11.84QFRR41 pKa = 11.84RR42 pKa = 11.84VTSGYY47 pKa = 9.73RR48 pKa = 11.84GYY50 pKa = 9.99PRR52 pKa = 11.84PLPTGGKK59 pKa = 6.8PTQKK63 pKa = 8.7TDD65 pKa = 2.93LRR67 pKa = 11.84FRR69 pKa = 11.84CNEE72 pKa = 3.75CGKK75 pKa = 9.72AHH77 pKa = 5.7SRR79 pKa = 11.84KK80 pKa = 9.76GFRR83 pKa = 11.84TSRR86 pKa = 11.84LEE88 pKa = 4.07LEE90 pKa = 4.58GG91 pKa = 4.2

Molecular weight: 10.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2152

0

2152

525482

26

1738

244.2

27.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.163 ± 0.047

0.959 ± 0.022

5.92 ± 0.05

9.305 ± 0.074

4.002 ± 0.046

6.789 ± 0.055

1.494 ± 0.021

8.466 ± 0.065

9.735 ± 0.088

9.145 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.999 ± 0.023

5.082 ± 0.043

3.54 ± 0.033

2.438 ± 0.031

4.293 ± 0.039

6.43 ± 0.044

4.828 ± 0.044

6.213 ± 0.051

0.899 ± 0.023

3.301 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski