Cardamine chlorotic fleck virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Betacarmovirus

Average proteome isoelectric point is 8.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q65988|Q65988_9TOMB p8 protein OS=Cardamine chlorotic fleck virus OX=31711 PE=4 SV=1
MM1 pKa = 8.06DD2 pKa = 5.48ALLPEE7 pKa = 4.61FLAHH11 pKa = 6.81KK12 pKa = 10.38LSRR15 pKa = 11.84LVLKK19 pKa = 10.67CFGIGSDD26 pKa = 4.09EE27 pKa = 4.34EE28 pKa = 4.32APQQIFVGEE37 pKa = 3.96FPVWGGEE44 pKa = 3.97IVPPQPEE51 pKa = 4.36EE52 pKa = 3.99LNQLVIPRR60 pKa = 11.84KK61 pKa = 6.81WVRR64 pKa = 11.84SIGSSLNRR72 pKa = 11.84VVLPPSSGVVASLEE86 pKa = 4.34EE87 pKa = 3.98NCVAPFEE94 pKa = 4.34EE95 pKa = 4.97ASVEE99 pKa = 4.06QLEE102 pKa = 4.42PASGDD107 pKa = 3.38TGDD110 pKa = 3.57LPRR113 pKa = 11.84RR114 pKa = 11.84KK115 pKa = 9.0IRR117 pKa = 11.84RR118 pKa = 11.84RR119 pKa = 11.84GRR121 pKa = 11.84FVAHH125 pKa = 6.53AVCAAKK131 pKa = 10.43LHH133 pKa = 6.26FSGCPTQTLANRR145 pKa = 11.84LAVSKK150 pKa = 10.16WLVQYY155 pKa = 8.95CTEE158 pKa = 4.12RR159 pKa = 11.84NVIPSHH165 pKa = 5.02TRR167 pKa = 11.84IAVQEE172 pKa = 4.02ALPQVFLPDD181 pKa = 3.22GHH183 pKa = 6.59DD184 pKa = 3.41LKK186 pKa = 11.16EE187 pKa = 4.09VLDD190 pKa = 3.83LHH192 pKa = 6.03TEE194 pKa = 4.01EE195 pKa = 4.63AHH197 pKa = 6.99DD198 pKa = 4.18RR199 pKa = 11.84RR200 pKa = 11.84NALRR204 pKa = 11.84KK205 pKa = 9.25RR206 pKa = 11.84GRR208 pKa = 11.84HH209 pKa = 3.67VRR211 pKa = 11.84RR212 pKa = 11.84WWVQLAMHH220 pKa = 7.12PMTGRR225 pKa = 11.84SWLRR229 pKa = 11.84AWRR232 pKa = 11.84RR233 pKa = 11.84LTGLPDD239 pKa = 3.56DD240 pKa = 4.17VAVQFVRR247 pKa = 11.84XGCLRR252 pKa = 11.84EE253 pKa = 3.9LVGRR257 pKa = 11.84EE258 pKa = 3.66TAISRR263 pKa = 11.84GEE265 pKa = 4.03HH266 pKa = 5.33PAMRR270 pKa = 11.84VFPLAQPPKK279 pKa = 9.18IRR281 pKa = 11.84RR282 pKa = 11.84IFHH285 pKa = 5.58ICGMGNGLDD294 pKa = 3.67FGVHH298 pKa = 5.81NNSLNNLRR306 pKa = 11.84RR307 pKa = 11.84GLMEE311 pKa = 3.52RR312 pKa = 11.84VFYY315 pKa = 11.45VEE317 pKa = 5.05DD318 pKa = 3.59AQKK321 pKa = 10.38EE322 pKa = 4.38LKK324 pKa = 9.72PAPQPLPDD332 pKa = 4.31AFAKK336 pKa = 10.7LSGIRR341 pKa = 11.84RR342 pKa = 11.84KK343 pKa = 9.47LVKK346 pKa = 10.25LAGNHH351 pKa = 4.65TPILRR356 pKa = 11.84EE357 pKa = 4.57DD358 pKa = 3.54YY359 pKa = 10.9SKK361 pKa = 11.1LYY363 pKa = 9.71KK364 pKa = 10.26GRR366 pKa = 11.84RR367 pKa = 11.84ATIYY371 pKa = 10.77QNACEE376 pKa = 4.19SLADD380 pKa = 4.1RR381 pKa = 11.84ACTQGGSAEE390 pKa = 4.24LKK392 pKa = 9.4TFVKK396 pKa = 10.54AEE398 pKa = 4.43KK399 pKa = 10.48INFTAKK405 pKa = 10.05KK406 pKa = 9.71DD407 pKa = 3.4PAPRR411 pKa = 11.84VIQPRR416 pKa = 11.84DD417 pKa = 3.13PRR419 pKa = 11.84YY420 pKa = 9.34NIEE423 pKa = 3.55VGKK426 pKa = 9.76YY427 pKa = 7.56LKK429 pKa = 10.49CYY431 pKa = 7.6EE432 pKa = 4.09HH433 pKa = 6.8HH434 pKa = 7.45LYY436 pKa = 10.48RR437 pKa = 11.84AIDD440 pKa = 3.78KK441 pKa = 7.23MWCGPTVLKK450 pKa = 10.52GYY452 pKa = 9.78NVEE455 pKa = 4.24EE456 pKa = 4.49IGNIMSDD463 pKa = 3.12AWNQFQKK470 pKa = 10.62PCAIGFDD477 pKa = 3.65MKK479 pKa = 10.85RR480 pKa = 11.84FDD482 pKa = 3.31QHH484 pKa = 8.09VSVDD488 pKa = 3.44ALKK491 pKa = 10.34WEE493 pKa = 4.08HH494 pKa = 5.97SVYY497 pKa = 10.36NASFNCPDD505 pKa = 3.86LAKK508 pKa = 10.26MLTWQLKK515 pKa = 8.38NKK517 pKa = 9.91GVGRR521 pKa = 11.84ASDD524 pKa = 4.59GYY526 pKa = 10.41IKK528 pKa = 10.74YY529 pKa = 9.75QVEE532 pKa = 4.47GCRR535 pKa = 11.84MSGDD539 pKa = 3.46VNTAMGNCLLACSITRR555 pKa = 11.84YY556 pKa = 9.66LMKK559 pKa = 10.5GIKK562 pKa = 9.5SRR564 pKa = 11.84LINNGDD570 pKa = 3.48DD571 pKa = 3.1CVVFFEE577 pKa = 4.71SGDD580 pKa = 3.34MHH582 pKa = 6.32EE583 pKa = 4.33VRR585 pKa = 11.84EE586 pKa = 4.26RR587 pKa = 11.84LCRR590 pKa = 11.84WIDD593 pKa = 3.6FGFQCIAEE601 pKa = 4.21EE602 pKa = 3.92PQYY605 pKa = 11.33DD606 pKa = 3.84LEE608 pKa = 4.55KK609 pKa = 11.26VEE611 pKa = 4.75FCQMSPIYY619 pKa = 10.43DD620 pKa = 3.55GEE622 pKa = 4.29GWVMVRR628 pKa = 11.84NPRR631 pKa = 11.84VSLSKK636 pKa = 10.67DD637 pKa = 3.38SYY639 pKa = 11.35STTQWGSVRR648 pKa = 11.84DD649 pKa = 3.74AASWLASIGEE659 pKa = 4.41CGMAIAGGIPIVQSYY674 pKa = 7.76YY675 pKa = 11.09NCLLRR680 pKa = 11.84NFKK683 pKa = 10.27HH684 pKa = 6.57LARR687 pKa = 11.84NTSKK691 pKa = 10.98KK692 pKa = 10.49KK693 pKa = 10.63LDD695 pKa = 3.31VSFNSGFSMLAQNGNRR711 pKa = 11.84GSKK714 pKa = 9.49EE715 pKa = 3.57VSEE718 pKa = 4.19AARR721 pKa = 11.84YY722 pKa = 9.0SFYY725 pKa = 11.33VAFGYY730 pKa = 8.92TPDD733 pKa = 3.61EE734 pKa = 3.95QRR736 pKa = 11.84GLEE739 pKa = 4.1GYY741 pKa = 10.08YY742 pKa = 10.99DD743 pKa = 3.86NLALEE748 pKa = 4.72AAWDD752 pKa = 3.77PEE754 pKa = 4.64GYY756 pKa = 10.46DD757 pKa = 3.35EE758 pKa = 4.58TLLNRR763 pKa = 11.84WIINPSLTTPP773 pKa = 3.72

Molecular weight: 87.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q65989|Q65989_9TOMB p9 protein OS=Cardamine chlorotic fleck virus OX=31711 PE=4 SV=1
MM1 pKa = 8.11DD2 pKa = 4.4NQSEE6 pKa = 4.4SDD8 pKa = 3.39NSVKK12 pKa = 10.16RR13 pKa = 11.84RR14 pKa = 11.84SGKK17 pKa = 9.81KK18 pKa = 8.45VNKK21 pKa = 8.25QVRR24 pKa = 11.84GSEE27 pKa = 3.72KK28 pKa = 10.1SATKK32 pKa = 10.37RR33 pKa = 11.84SVASHH38 pKa = 6.93AARR41 pKa = 11.84SFSQRR46 pKa = 11.84PSGDD50 pKa = 3.3SAGGNWVLVADD61 pKa = 5.01KK62 pKa = 10.73IEE64 pKa = 3.97VSISFNFF71 pKa = 3.34

Molecular weight: 7.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1289

71

773

322.3

35.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.991 ± 0.633

2.095 ± 0.71

4.577 ± 0.348

5.586 ± 1.102

3.801 ± 0.105

7.292 ± 0.694

2.095 ± 0.424

4.577 ± 0.758

5.818 ± 0.669

8.999 ± 1.264

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.784 ± 0.494

4.422 ± 0.633

5.431 ± 0.494

3.646 ± 0.214

6.827 ± 1.064

7.758 ± 2.255

4.81 ± 1.653

7.448 ± 0.521

2.017 ± 0.18

2.948 ± 0.441
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski