Streptococcus satellite phage Javan295

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZNM3|A0A4D5ZNM3_9VIRU Transcriptional regulator OS=Streptococcus satellite phage Javan295 OX=2558614 GN=JavanS295_0002 PE=4 SV=1
MM1 pKa = 7.5SISEE5 pKa = 4.26FAKK8 pKa = 10.6KK9 pKa = 10.6LPDD12 pKa = 4.57DD13 pKa = 3.99NTTLSLGYY21 pKa = 9.87EE22 pKa = 4.22KK23 pKa = 10.93SADD26 pKa = 3.72QYY28 pKa = 12.19DD29 pKa = 5.06LIAKK33 pKa = 9.82SSKK36 pKa = 10.19QKK38 pKa = 11.06DD39 pKa = 4.1FIPVGEE45 pKa = 4.55IIVNN49 pKa = 4.02

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZKH3|A0A4D5ZKH3_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan295 OX=2558614 GN=JavanS295_0011 PE=4 SV=1
MM1 pKa = 7.9DD2 pKa = 5.64LAAQLANILAEE13 pKa = 4.04YY14 pKa = 10.08CEE16 pKa = 4.32EE17 pKa = 4.1VNEE20 pKa = 4.26EE21 pKa = 3.64VDD23 pKa = 4.71KK24 pKa = 11.22IAEE27 pKa = 4.18QVAKK31 pKa = 9.33EE32 pKa = 4.16TVKK35 pKa = 10.43EE36 pKa = 3.96LRR38 pKa = 11.84EE39 pKa = 3.99TSPKK43 pKa = 9.52RR44 pKa = 11.84TGKK47 pKa = 10.24YY48 pKa = 9.18SKK50 pKa = 9.74GWRR53 pKa = 11.84KK54 pKa = 9.52KK55 pKa = 9.16RR56 pKa = 11.84VRR58 pKa = 11.84NGVWVVYY65 pKa = 8.83SFKK68 pKa = 10.96YY69 pKa = 10.71GSLTHH74 pKa = 6.73LLEE77 pKa = 4.86FGHH80 pKa = 6.93IKK82 pKa = 10.57RR83 pKa = 11.84NGGRR87 pKa = 11.84TKK89 pKa = 10.28AYY91 pKa = 8.33PHH93 pKa = 6.61LRR95 pKa = 11.84PAEE98 pKa = 3.9LNAIQKK104 pKa = 7.51FTEE107 pKa = 4.66RR108 pKa = 11.84IKK110 pKa = 11.09NISKK114 pKa = 10.68

Molecular weight: 13.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18

0

18

2845

49

388

158.1

18.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.605 ± 0.398

0.562 ± 0.117

6.467 ± 0.575

8.366 ± 0.601

4.429 ± 0.28

4.569 ± 0.478

1.441 ± 0.298

6.749 ± 0.35

11.353 ± 0.498

9.701 ± 0.292

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.004 ± 0.273

6.116 ± 0.44

2.109 ± 0.283

4.112 ± 0.361

4.499 ± 0.37

6.186 ± 0.363

6.116 ± 0.575

5.272 ± 0.381

0.808 ± 0.109

4.534 ± 0.311
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski