Amino acid dipepetide frequency for Klebsiella phage KMI1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.669AlaAla: 9.669 ± 1.078
0.42AlaCys: 0.42 ± 0.186
6.474AlaAsp: 6.474 ± 0.789
5.549AlaGlu: 5.549 ± 0.852
2.606AlaPhe: 2.606 ± 0.481
7.146AlaGly: 7.146 ± 1.245
1.597AlaHis: 1.597 ± 0.342
4.456AlaIle: 4.456 ± 0.467
6.138AlaLys: 6.138 ± 0.685
7.903AlaLeu: 7.903 ± 1.008
2.606AlaMet: 2.606 ± 0.761
4.12AlaAsn: 4.12 ± 0.476
2.943AlaPro: 2.943 ± 0.62
3.783AlaGln: 3.783 ± 0.588
4.624AlaArg: 4.624 ± 0.607
6.053AlaSer: 6.053 ± 0.812
4.036AlaThr: 4.036 ± 0.527
5.465AlaVal: 5.465 ± 0.651
1.093AlaTrp: 1.093 ± 0.336
2.606AlaTyr: 2.606 ± 0.412
0.0AlaXaa: 0.0 ± 0.0
Cys
0.504CysAla: 0.504 ± 0.226
0.084CysCys: 0.084 ± 0.082
0.757CysAsp: 0.757 ± 0.28
0.673CysGlu: 0.673 ± 0.233
0.504CysPhe: 0.504 ± 0.254
0.589CysGly: 0.589 ± 0.204
0.168CysHis: 0.168 ± 0.103
0.757CysIle: 0.757 ± 0.254
0.252CysLys: 0.252 ± 0.124
0.925CysLeu: 0.925 ± 0.269
0.0CysMet: 0.0 ± 0.0
0.252CysAsn: 0.252 ± 0.177
0.42CysPro: 0.42 ± 0.185
0.42CysGln: 0.42 ± 0.214
0.589CysArg: 0.589 ± 0.264
0.589CysSer: 0.589 ± 0.252
0.504CysThr: 0.504 ± 0.216
0.925CysVal: 0.925 ± 0.236
0.084CysTrp: 0.084 ± 0.081
0.168CysTyr: 0.168 ± 0.141
0.0CysXaa: 0.0 ± 0.0
Asp
5.801AspAla: 5.801 ± 0.59
0.673AspCys: 0.673 ± 0.251
3.952AspAsp: 3.952 ± 0.559
3.027AspGlu: 3.027 ± 0.553
2.69AspPhe: 2.69 ± 0.458
6.81AspGly: 6.81 ± 0.611
0.841AspHis: 0.841 ± 0.284
3.531AspIle: 3.531 ± 0.633
4.036AspLys: 4.036 ± 0.628
3.531AspLeu: 3.531 ± 0.654
1.682AspMet: 1.682 ± 0.347
2.69AspAsn: 2.69 ± 0.483
2.859AspPro: 2.859 ± 0.487
2.606AspGln: 2.606 ± 0.512
2.69AspArg: 2.69 ± 0.393
3.195AspSer: 3.195 ± 0.508
4.708AspThr: 4.708 ± 0.499
4.372AspVal: 4.372 ± 0.554
0.925AspTrp: 0.925 ± 0.348
2.354AspTyr: 2.354 ± 0.448
0.0AspXaa: 0.0 ± 0.0
Glu
7.399GluAla: 7.399 ± 0.938
0.42GluCys: 0.42 ± 0.236
4.54GluAsp: 4.54 ± 0.755
5.969GluGlu: 5.969 ± 1.339
2.69GluPhe: 2.69 ± 0.409
5.801GluGly: 5.801 ± 0.998
1.261GluHis: 1.261 ± 0.495
2.606GluIle: 2.606 ± 0.389
3.279GluLys: 3.279 ± 0.72
5.885GluLeu: 5.885 ± 0.868
1.766GluMet: 1.766 ± 0.6
2.606GluAsn: 2.606 ± 0.439
1.85GluPro: 1.85 ± 0.552
2.775GluGln: 2.775 ± 0.694
3.447GluArg: 3.447 ± 0.613
4.12GluSer: 4.12 ± 0.723
3.195GluThr: 3.195 ± 0.554
4.876GluVal: 4.876 ± 0.701
0.42GluTrp: 0.42 ± 0.159
3.279GluTyr: 3.279 ± 0.379
0.0GluXaa: 0.0 ± 0.0
Phe
2.522PheAla: 2.522 ± 0.43
0.252PheCys: 0.252 ± 0.169
2.606PheAsp: 2.606 ± 0.4
1.597PheGlu: 1.597 ± 0.306
0.841PhePhe: 0.841 ± 0.241
3.195PheGly: 3.195 ± 0.593
0.589PheHis: 0.589 ± 0.277
2.27PheIle: 2.27 ± 0.541
2.606PheLys: 2.606 ± 0.452
3.111PheLeu: 3.111 ± 0.461
1.093PheMet: 1.093 ± 0.287
2.018PheAsn: 2.018 ± 0.408
1.766PhePro: 1.766 ± 0.357
1.177PheGln: 1.177 ± 0.338
1.85PheArg: 1.85 ± 0.486
2.438PheSer: 2.438 ± 0.474
2.606PheThr: 2.606 ± 0.451
1.85PheVal: 1.85 ± 0.35
0.336PheTrp: 0.336 ± 0.132
1.009PheTyr: 1.009 ± 0.261
0.0PheXaa: 0.0 ± 0.0
Gly
6.642GlyAla: 6.642 ± 1.006
0.673GlyCys: 0.673 ± 0.252
5.465GlyAsp: 5.465 ± 0.532
5.465GlyGlu: 5.465 ± 0.752
2.606GlyPhe: 2.606 ± 0.362
5.717GlyGly: 5.717 ± 0.641
1.429GlyHis: 1.429 ± 0.366
4.624GlyIle: 4.624 ± 0.956
5.381GlyLys: 5.381 ± 0.864
6.138GlyLeu: 6.138 ± 0.827
1.682GlyMet: 1.682 ± 0.447
3.447GlyAsn: 3.447 ± 0.614
1.177GlyPro: 1.177 ± 0.396
2.943GlyGln: 2.943 ± 0.62
4.708GlyArg: 4.708 ± 0.354
6.81GlySer: 6.81 ± 0.855
5.129GlyThr: 5.129 ± 0.787
6.138GlyVal: 6.138 ± 0.998
1.597GlyTrp: 1.597 ± 0.426
3.363GlyTyr: 3.363 ± 0.452
0.0GlyXaa: 0.0 ± 0.0
His
1.177HisAla: 1.177 ± 0.283
0.589HisCys: 0.589 ± 0.276
1.177HisAsp: 1.177 ± 0.27
1.009HisGlu: 1.009 ± 0.356
0.841HisPhe: 0.841 ± 0.232
1.682HisGly: 1.682 ± 0.435
0.504HisHis: 0.504 ± 0.236
1.429HisIle: 1.429 ± 0.315
1.177HisLys: 1.177 ± 0.335
1.597HisLeu: 1.597 ± 0.425
0.589HisMet: 0.589 ± 0.226
0.504HisAsn: 0.504 ± 0.242
0.757HisPro: 0.757 ± 0.166
0.42HisGln: 0.42 ± 0.208
0.757HisArg: 0.757 ± 0.223
0.673HisSer: 0.673 ± 0.236
1.009HisThr: 1.009 ± 0.237
1.345HisVal: 1.345 ± 0.281
0.168HisTrp: 0.168 ± 0.107
0.589HisTyr: 0.589 ± 0.163
0.0HisXaa: 0.0 ± 0.0
Ile
4.54IleAla: 4.54 ± 0.533
0.589IleCys: 0.589 ± 0.218
3.531IleAsp: 3.531 ± 0.507
3.195IleGlu: 3.195 ± 0.497
0.925IlePhe: 0.925 ± 0.277
4.204IleGly: 4.204 ± 0.488
0.757IleHis: 0.757 ± 0.276
2.606IleIle: 2.606 ± 0.549
3.111IleLys: 3.111 ± 0.468
3.699IleLeu: 3.699 ± 0.438
1.093IleMet: 1.093 ± 0.376
2.102IleAsn: 2.102 ± 0.669
2.438IlePro: 2.438 ± 0.524
1.345IleGln: 1.345 ± 0.385
3.867IleArg: 3.867 ± 0.631
3.195IleSer: 3.195 ± 0.488
2.775IleThr: 2.775 ± 0.668
3.111IleVal: 3.111 ± 0.503
0.504IleTrp: 0.504 ± 0.219
1.934IleTyr: 1.934 ± 0.487
0.0IleXaa: 0.0 ± 0.0
Lys
6.726LysAla: 6.726 ± 1.025
0.589LysCys: 0.589 ± 0.228
3.363LysAsp: 3.363 ± 0.608
5.381LysGlu: 5.381 ± 0.606
2.102LysPhe: 2.102 ± 0.455
5.717LysGly: 5.717 ± 0.914
1.597LysHis: 1.597 ± 0.326
2.354LysIle: 2.354 ± 0.505
3.447LysLys: 3.447 ± 0.87
5.717LysLeu: 5.717 ± 0.692
1.513LysMet: 1.513 ± 0.335
2.943LysAsn: 2.943 ± 0.431
2.69LysPro: 2.69 ± 0.614
2.438LysGln: 2.438 ± 0.483
3.279LysArg: 3.279 ± 0.716
3.867LysSer: 3.867 ± 0.542
3.279LysThr: 3.279 ± 0.432
4.96LysVal: 4.96 ± 0.799
0.757LysTrp: 0.757 ± 0.24
2.186LysTyr: 2.186 ± 0.461
0.0LysXaa: 0.0 ± 0.0
Leu
7.062LeuAla: 7.062 ± 1.097
0.504LeuCys: 0.504 ± 0.211
4.456LeuAsp: 4.456 ± 0.634
6.726LeuGlu: 6.726 ± 1.11
2.522LeuPhe: 2.522 ± 0.446
5.213LeuGly: 5.213 ± 0.633
1.345LeuHis: 1.345 ± 0.359
3.867LeuIle: 3.867 ± 0.624
6.558LeuLys: 6.558 ± 0.641
5.969LeuLeu: 5.969 ± 0.799
2.354LeuMet: 2.354 ± 0.309
3.783LeuAsn: 3.783 ± 0.508
2.775LeuPro: 2.775 ± 0.544
3.363LeuGln: 3.363 ± 0.532
5.717LeuArg: 5.717 ± 0.626
4.96LeuSer: 4.96 ± 0.601
4.54LeuThr: 4.54 ± 0.681
4.876LeuVal: 4.876 ± 0.623
1.093LeuTrp: 1.093 ± 0.372
2.775LeuTyr: 2.775 ± 0.478
0.0LeuXaa: 0.0 ± 0.0
Met
3.279MetAla: 3.279 ± 0.458
0.168MetCys: 0.168 ± 0.128
2.102MetAsp: 2.102 ± 0.362
1.177MetGlu: 1.177 ± 0.359
0.841MetPhe: 0.841 ± 0.259
1.261MetGly: 1.261 ± 0.329
0.504MetHis: 0.504 ± 0.218
0.504MetIle: 0.504 ± 0.192
1.177MetLys: 1.177 ± 0.311
2.69MetLeu: 2.69 ± 0.554
0.673MetMet: 0.673 ± 0.204
0.757MetAsn: 0.757 ± 0.233
0.925MetPro: 0.925 ± 0.255
1.934MetGln: 1.934 ± 0.418
1.093MetArg: 1.093 ± 0.224
1.513MetSer: 1.513 ± 0.377
1.934MetThr: 1.934 ± 0.423
1.85MetVal: 1.85 ± 0.492
0.336MetTrp: 0.336 ± 0.174
0.589MetTyr: 0.589 ± 0.22
0.0MetXaa: 0.0 ± 0.0
Asn
3.867AsnAla: 3.867 ± 0.69
0.42AsnCys: 0.42 ± 0.239
2.102AsnAsp: 2.102 ± 0.302
2.859AsnGlu: 2.859 ± 0.573
1.597AsnPhe: 1.597 ± 0.286
4.288AsnGly: 4.288 ± 0.708
0.252AsnHis: 0.252 ± 0.137
2.354AsnIle: 2.354 ± 0.48
1.682AsnLys: 1.682 ± 0.419
2.69AsnLeu: 2.69 ± 0.47
1.093AsnMet: 1.093 ± 0.357
1.597AsnAsn: 1.597 ± 0.303
2.27AsnPro: 2.27 ± 0.351
1.261AsnGln: 1.261 ± 0.27
2.354AsnArg: 2.354 ± 0.689
3.111AsnSer: 3.111 ± 0.418
1.682AsnThr: 1.682 ± 0.301
3.699AsnVal: 3.699 ± 1.047
0.589AsnTrp: 0.589 ± 0.217
1.85AsnTyr: 1.85 ± 0.421
0.0AsnXaa: 0.0 ± 0.0
Pro
3.111ProAla: 3.111 ± 0.42
0.757ProCys: 0.757 ± 0.207
2.018ProAsp: 2.018 ± 0.406
4.372ProGlu: 4.372 ± 0.754
1.429ProPhe: 1.429 ± 0.271
2.354ProGly: 2.354 ± 0.528
0.504ProHis: 0.504 ± 0.209
1.261ProIle: 1.261 ± 0.355
2.69ProLys: 2.69 ± 0.466
2.522ProLeu: 2.522 ± 0.366
0.841ProMet: 0.841 ± 0.301
2.018ProAsn: 2.018 ± 0.419
1.009ProPro: 1.009 ± 0.363
1.345ProGln: 1.345 ± 0.308
1.682ProArg: 1.682 ± 0.391
2.018ProSer: 2.018 ± 0.31
1.513ProThr: 1.513 ± 0.368
3.111ProVal: 3.111 ± 0.403
0.757ProTrp: 0.757 ± 0.211
1.597ProTyr: 1.597 ± 0.463
0.0ProXaa: 0.0 ± 0.0
Gln
3.615GlnAla: 3.615 ± 0.585
0.084GlnCys: 0.084 ± 0.075
2.27GlnAsp: 2.27 ± 0.344
2.943GlnGlu: 2.943 ± 0.413
1.934GlnPhe: 1.934 ± 0.395
2.522GlnGly: 2.522 ± 0.447
0.504GlnHis: 0.504 ± 0.214
1.766GlnIle: 1.766 ± 0.434
3.279GlnLys: 3.279 ± 0.5
3.867GlnLeu: 3.867 ± 0.453
1.261GlnMet: 1.261 ± 0.498
1.261GlnAsn: 1.261 ± 0.252
1.934GlnPro: 1.934 ± 0.356
2.859GlnGln: 2.859 ± 0.74
2.102GlnArg: 2.102 ± 0.516
2.606GlnSer: 2.606 ± 0.476
1.766GlnThr: 1.766 ± 0.428
2.27GlnVal: 2.27 ± 0.425
0.757GlnTrp: 0.757 ± 0.264
1.682GlnTyr: 1.682 ± 0.521
0.0GlnXaa: 0.0 ± 0.0
Arg
4.708ArgAla: 4.708 ± 0.756
0.673ArgCys: 0.673 ± 0.281
3.615ArgAsp: 3.615 ± 0.443
3.615ArgGlu: 3.615 ± 0.56
1.934ArgPhe: 1.934 ± 0.516
4.036ArgGly: 4.036 ± 0.588
0.841ArgHis: 0.841 ± 0.219
3.111ArgIle: 3.111 ± 0.432
3.952ArgLys: 3.952 ± 0.721
5.045ArgLeu: 5.045 ± 0.675
1.177ArgMet: 1.177 ± 0.252
1.85ArgAsn: 1.85 ± 0.385
2.018ArgPro: 2.018 ± 0.399
2.606ArgGln: 2.606 ± 0.465
2.775ArgArg: 2.775 ± 0.41
4.372ArgSer: 4.372 ± 0.53
2.859ArgThr: 2.859 ± 0.519
3.699ArgVal: 3.699 ± 0.589
1.009ArgTrp: 1.009 ± 0.335
1.345ArgTyr: 1.345 ± 0.234
0.0ArgXaa: 0.0 ± 0.0
Ser
5.465SerAla: 5.465 ± 0.772
0.504SerCys: 0.504 ± 0.184
3.952SerAsp: 3.952 ± 0.52
4.036SerGlu: 4.036 ± 0.507
3.027SerPhe: 3.027 ± 0.473
6.138SerGly: 6.138 ± 1.214
2.018SerHis: 2.018 ± 0.42
2.438SerIle: 2.438 ± 0.482
4.372SerLys: 4.372 ± 0.519
5.213SerLeu: 5.213 ± 0.775
1.513SerMet: 1.513 ± 0.335
1.85SerAsn: 1.85 ± 0.479
2.27SerPro: 2.27 ± 0.383
2.775SerGln: 2.775 ± 0.479
3.531SerArg: 3.531 ± 0.571
3.531SerSer: 3.531 ± 0.692
4.456SerThr: 4.456 ± 0.791
4.96SerVal: 4.96 ± 0.728
0.589SerTrp: 0.589 ± 0.203
2.27SerTyr: 2.27 ± 0.604
0.0SerXaa: 0.0 ± 0.0
Thr
4.708ThrAla: 4.708 ± 0.809
0.673ThrCys: 0.673 ± 0.235
2.775ThrAsp: 2.775 ± 0.504
3.615ThrGlu: 3.615 ± 0.548
2.859ThrPhe: 2.859 ± 0.548
5.969ThrGly: 5.969 ± 0.787
1.009ThrHis: 1.009 ± 0.217
3.699ThrIle: 3.699 ± 0.481
4.456ThrLys: 4.456 ± 0.616
5.045ThrLeu: 5.045 ± 0.663
1.429ThrMet: 1.429 ± 0.293
2.102ThrAsn: 2.102 ± 0.579
2.522ThrPro: 2.522 ± 0.472
2.186ThrGln: 2.186 ± 0.408
2.354ThrArg: 2.354 ± 0.374
4.288ThrSer: 4.288 ± 0.868
3.027ThrThr: 3.027 ± 0.692
3.699ThrVal: 3.699 ± 0.752
0.42ThrTrp: 0.42 ± 0.178
1.513ThrTyr: 1.513 ± 0.342
0.0ThrXaa: 0.0 ± 0.0
Val
5.465ValAla: 5.465 ± 0.732
0.504ValCys: 0.504 ± 0.204
4.204ValAsp: 4.204 ± 0.569
3.952ValGlu: 3.952 ± 0.679
2.438ValPhe: 2.438 ± 0.601
4.792ValGly: 4.792 ± 0.481
1.345ValHis: 1.345 ± 0.397
3.867ValIle: 3.867 ± 0.565
4.036ValLys: 4.036 ± 0.581
5.129ValLeu: 5.129 ± 0.638
1.177ValMet: 1.177 ± 0.269
2.943ValAsn: 2.943 ± 0.77
3.111ValPro: 3.111 ± 0.51
2.775ValGln: 2.775 ± 0.432
5.045ValArg: 5.045 ± 0.715
4.96ValSer: 4.96 ± 0.868
6.222ValThr: 6.222 ± 0.895
4.288ValVal: 4.288 ± 0.574
0.841ValTrp: 0.841 ± 0.31
2.522ValTyr: 2.522 ± 0.563
0.0ValXaa: 0.0 ± 0.0
Trp
0.42TrpAla: 0.42 ± 0.168
0.336TrpCys: 0.336 ± 0.154
0.673TrpAsp: 0.673 ± 0.241
1.009TrpGlu: 1.009 ± 0.249
0.42TrpPhe: 0.42 ± 0.194
0.589TrpGly: 0.589 ± 0.249
0.336TrpHis: 0.336 ± 0.188
0.589TrpIle: 0.589 ± 0.288
1.093TrpLys: 1.093 ± 0.261
1.429TrpLeu: 1.429 ± 0.411
0.42TrpMet: 0.42 ± 0.196
0.589TrpAsn: 0.589 ± 0.214
0.252TrpPro: 0.252 ± 0.143
0.589TrpGln: 0.589 ± 0.191
0.673TrpArg: 0.673 ± 0.249
0.925TrpSer: 0.925 ± 0.364
0.841TrpThr: 0.841 ± 0.242
1.345TrpVal: 1.345 ± 0.35
0.252TrpTrp: 0.252 ± 0.137
0.168TrpTyr: 0.168 ± 0.111
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.606TyrAla: 2.606 ± 0.466
0.252TyrCys: 0.252 ± 0.145
2.943TyrAsp: 2.943 ± 0.53
2.018TyrGlu: 2.018 ± 0.509
0.925TyrPhe: 0.925 ± 0.283
3.111TyrGly: 3.111 ± 0.537
0.589TyrHis: 0.589 ± 0.241
1.429TyrIle: 1.429 ± 0.404
2.186TyrLys: 2.186 ± 0.33
2.354TyrLeu: 2.354 ± 0.313
1.261TyrMet: 1.261 ± 0.316
2.186TyrAsn: 2.186 ± 0.408
0.925TyrPro: 0.925 ± 0.268
1.682TyrGln: 1.682 ± 0.474
2.102TyrArg: 2.102 ± 0.391
1.682TyrSer: 1.682 ± 0.366
2.27TyrThr: 2.27 ± 0.56
2.859TyrVal: 2.859 ± 0.531
0.42TyrTrp: 0.42 ± 0.217
0.673TyrTyr: 0.673 ± 0.342
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 41 proteins (11895 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski