Pseudoclavibacter endophyticus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Pseudoclavibacter

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2822 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6H9WQS1|A0A6H9WQS1_9MICO ABC transporter substrate-binding protein OS=Pseudoclavibacter endophyticus OX=1778590 GN=F8O04_02310 PE=4 SV=1
MM1 pKa = 7.23TRR3 pKa = 11.84LGPAIACLVALAVALAGCSHH23 pKa = 7.29ANPAGAGAADD33 pKa = 4.31GADD36 pKa = 3.63AAPGTAAADD45 pKa = 3.58TGGAGAGLDD54 pKa = 3.72VYY56 pKa = 10.97ATTGYY61 pKa = 10.42LADD64 pKa = 5.74AIANIAPGATVTTMVGPGGDD84 pKa = 3.55PHH86 pKa = 6.14THH88 pKa = 5.38QPSTRR93 pKa = 11.84DD94 pKa = 2.85IEE96 pKa = 5.21AILDD100 pKa = 3.69ADD102 pKa = 3.94VVFWNGLHH110 pKa = 7.07LEE112 pKa = 4.14AQMVDD117 pKa = 3.11QLTSLGDD124 pKa = 3.34RR125 pKa = 11.84QLAVGDD131 pKa = 4.03RR132 pKa = 11.84LPDD135 pKa = 3.67GVLLDD140 pKa = 4.33WPDD143 pKa = 3.12TDD145 pKa = 4.74ADD147 pKa = 4.04GNALHH152 pKa = 7.45DD153 pKa = 3.7PHH155 pKa = 7.77VWNSPTAWSLVVGLIADD172 pKa = 4.19KK173 pKa = 11.12LSALDD178 pKa = 4.02PSDD181 pKa = 3.42AATYY185 pKa = 9.6AANADD190 pKa = 3.69AYY192 pKa = 9.93VAEE195 pKa = 4.48IASAAEE201 pKa = 3.69NAEE204 pKa = 4.08RR205 pKa = 11.84MLATVPEE212 pKa = 4.18PRR214 pKa = 11.84ILITGHH220 pKa = 6.98DD221 pKa = 3.45AFNYY225 pKa = 10.02FGATYY230 pKa = 10.69DD231 pKa = 3.75LDD233 pKa = 4.28VYY235 pKa = 10.77ATDD238 pKa = 4.76FVSSDD243 pKa = 3.28AALSPQEE250 pKa = 3.99LSGLAGLIAEE260 pKa = 4.57HH261 pKa = 5.52QVPVIFMDD269 pKa = 3.71NQANPQAITSLQEE282 pKa = 3.54AVQARR287 pKa = 11.84GWQVAVADD295 pKa = 4.04AEE297 pKa = 4.58LYY299 pKa = 10.82ADD301 pKa = 4.22SLGAEE306 pKa = 4.48PGVDD310 pKa = 3.01TYY312 pKa = 11.75LGVLAHH318 pKa = 6.49NAAAIADD325 pKa = 4.0ALGEE329 pKa = 4.2EE330 pKa = 4.7PAA332 pKa = 4.79

Molecular weight: 33.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6H9WRD1|A0A6H9WRD1_9MICO WYL domain-containing protein OS=Pseudoclavibacter endophyticus OX=1778590 GN=F8O04_08330 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.45VHH17 pKa = 5.31GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.75GRR40 pKa = 11.84AKK42 pKa = 10.68LSAA45 pKa = 3.92

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2822

0

2822

967923

32

1790

343.0

36.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.476 ± 0.075

0.541 ± 0.012

6.375 ± 0.04

5.991 ± 0.044

3.03 ± 0.027

9.331 ± 0.041

2.074 ± 0.022

4.41 ± 0.033

1.652 ± 0.027

9.641 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.862 ± 0.018

1.968 ± 0.024

5.622 ± 0.036

2.708 ± 0.026

7.822 ± 0.064

5.54 ± 0.029

5.987 ± 0.038

8.721 ± 0.043

1.372 ± 0.021

1.875 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski