Pseudomonas phage Persinger

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D7ISZ3|A0A7D7ISZ3_9CAUD Uncharacterized protein OS=Pseudomonas phage Persinger OX=2749430 PE=4 SV=1
MM1 pKa = 7.39SLTYY5 pKa = 9.33RR6 pKa = 11.84TSDD9 pKa = 3.4GDD11 pKa = 3.77TADD14 pKa = 3.97YY15 pKa = 10.59IAWKK19 pKa = 10.67YY20 pKa = 11.22YY21 pKa = 8.13GTQDD25 pKa = 3.53GQVVEE30 pKa = 4.71QLLEE34 pKa = 4.42ANPGLADD41 pKa = 5.04LGPVLPAGVLVTLPEE56 pKa = 4.5LEE58 pKa = 4.56PATAAQVVRR67 pKa = 11.84LWDD70 pKa = 3.33

Molecular weight: 7.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D7IT00|A0A7D7IT00_9CAUD Terminase small subunit OS=Pseudomonas phage Persinger OX=2749430 PE=4 SV=1
MM1 pKa = 7.19NAAEE5 pKa = 4.12FAKK8 pKa = 9.75RR9 pKa = 11.84RR10 pKa = 11.84ARR12 pKa = 11.84IEE14 pKa = 4.01HH15 pKa = 4.82EE16 pKa = 3.67HH17 pKa = 5.37GRR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84GYY23 pKa = 9.21IAKK26 pKa = 10.42AMAALVRR33 pKa = 11.84DD34 pKa = 4.21AGAAIIIVHH43 pKa = 6.19GKK45 pKa = 8.89AVAWRR50 pKa = 11.84LPNGQIVCRR59 pKa = 11.84KK60 pKa = 8.5RR61 pKa = 11.84RR62 pKa = 11.84YY63 pKa = 8.67RR64 pKa = 11.84TEE66 pKa = 4.02LDD68 pKa = 2.97AMIALGHH75 pKa = 6.47IRR77 pKa = 11.84HH78 pKa = 6.63DD79 pKa = 3.9PKK81 pKa = 10.65TPKK84 pKa = 10.11IPTRR88 pKa = 11.84FYY90 pKa = 11.27LCPHH94 pKa = 6.44CNGWHH99 pKa = 6.9LSSQAPANDD108 pKa = 3.27NNAQQ112 pKa = 3.32

Molecular weight: 12.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

13251

48

781

210.3

22.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.422 ± 0.534

1.14 ± 0.141

6.128 ± 0.234

6.135 ± 0.312

2.913 ± 0.143

8.671 ± 0.329

1.721 ± 0.144

4.385 ± 0.225

4.52 ± 0.222

8.43 ± 0.222

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.37 ± 0.144

3.479 ± 0.208

4.422 ± 0.242

4.709 ± 0.222

7.056 ± 0.334

4.543 ± 0.175

5.894 ± 0.296

7.177 ± 0.297

1.743 ± 0.147

2.143 ± 0.145
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski