Calothrix sp. 336/3

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Calotrichaceae; Calothrix; unclassified Calothrix

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5001 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0T7BLG9|A0A0T7BLG9_9CYAN Clp protease ClpX OS=Calothrix sp. 336/3 OX=1337936 GN=IJ00_00170 PE=3 SV=1
MM1 pKa = 7.78SDD3 pKa = 2.89SLMYY7 pKa = 8.59QQEE10 pKa = 4.49YY11 pKa = 10.08FVVLEE16 pKa = 4.35TNQPEE21 pKa = 4.05QFLTVGEE28 pKa = 4.97LIAKK32 pKa = 8.32LQEE35 pKa = 4.29VIQNLDD41 pKa = 3.65AQDD44 pKa = 4.57LPPDD48 pKa = 4.37LSSLDD53 pKa = 3.67SPEE56 pKa = 4.45AQAQHH61 pKa = 6.62LVDD64 pKa = 4.44TSCEE68 pKa = 3.85LDD70 pKa = 3.31IGPGEE75 pKa = 4.03YY76 pKa = 10.25LQWYY80 pKa = 8.97AVRR83 pKa = 11.84LEE85 pKa = 4.13KK86 pKa = 10.85

Molecular weight: 9.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0T7BV16|A0A0T7BV16_9CYAN Thylakoid-associated protein OS=Calothrix sp. 336/3 OX=1337936 GN=IJ00_18275 PE=4 SV=1
MM1 pKa = 7.47TIEE4 pKa = 5.55PIPQKK9 pKa = 10.37KK10 pKa = 9.1YY11 pKa = 9.64PRR13 pKa = 11.84VQIGRR18 pKa = 11.84RR19 pKa = 11.84AMALGIDD26 pKa = 5.08FLGTWLLSSLLATGDD41 pKa = 3.23VGIQFLQILVFIFAWLGFRR60 pKa = 11.84VLLVYY65 pKa = 10.81SNQGQSLGKK74 pKa = 8.33WAFDD78 pKa = 3.19IKK80 pKa = 10.96VLEE83 pKa = 4.32SEE85 pKa = 4.34RR86 pKa = 11.84GRR88 pKa = 11.84VPDD91 pKa = 5.24LPTLLKK97 pKa = 10.56RR98 pKa = 11.84EE99 pKa = 4.61AIVGASALLVAIAWNNILRR118 pKa = 11.84NPTAILLMLPLAIDD132 pKa = 3.99CGAAVSDD139 pKa = 3.82TQFRR143 pKa = 11.84QALHH147 pKa = 6.6DD148 pKa = 4.43RR149 pKa = 11.84YY150 pKa = 10.05AGTMIVSSRR159 pKa = 11.84RR160 pKa = 11.84GYY162 pKa = 10.95SLDD165 pKa = 3.27IKK167 pKa = 10.19VKK169 pKa = 10.38RR170 pKa = 11.84LVEE173 pKa = 3.73TWRR176 pKa = 11.84RR177 pKa = 11.84NVRR180 pKa = 11.84RR181 pKa = 3.71

Molecular weight: 20.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5001

0

5001

1597472

31

2362

319.4

35.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.945 ± 0.038

1.002 ± 0.011

4.733 ± 0.024

6.156 ± 0.039

3.973 ± 0.024

6.69 ± 0.038

1.89 ± 0.016

7.099 ± 0.033

4.921 ± 0.028

10.742 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.938 ± 0.015

4.501 ± 0.033

4.696 ± 0.026

5.486 ± 0.03

4.982 ± 0.026

6.39 ± 0.031

5.778 ± 0.025

6.517 ± 0.028

1.441 ± 0.017

3.121 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski