Vanilla virus X

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Potexvirus

Average proteome isoelectric point is 7.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A220NQ60|A0A220NQ60_9VIRU Triple gene block protein 2 OS=Vanilla virus X OX=2016427 GN=TGBp2 PE=4 SV=1
MM1 pKa = 7.43ANIRR5 pKa = 11.84AALDD9 pKa = 3.48RR10 pKa = 11.84FTDD13 pKa = 3.67TSIKK17 pKa = 10.51SALQEE22 pKa = 3.76QAYY25 pKa = 10.87SDD27 pKa = 3.84VKK29 pKa = 10.98PEE31 pKa = 3.69LRR33 pKa = 11.84AINSLNPYY41 pKa = 8.97AQSTSGADD49 pKa = 3.5DD50 pKa = 4.44LEE52 pKa = 4.16QLGIGTNPFSIKK64 pKa = 10.4LHH66 pKa = 4.52THH68 pKa = 6.18AAAKK72 pKa = 10.11AVEE75 pKa = 3.89NRR77 pKa = 11.84MLEE80 pKa = 4.18IVGHH84 pKa = 5.71HH85 pKa = 6.93LPRR88 pKa = 11.84DD89 pKa = 3.38SCTFLWLKK97 pKa = 9.87KK98 pKa = 10.59AKK100 pKa = 10.54LNLLRR105 pKa = 11.84RR106 pKa = 11.84NNPLDD111 pKa = 3.26TFRR114 pKa = 11.84NVPFEE119 pKa = 4.49PKK121 pKa = 10.3DD122 pKa = 3.25FARR125 pKa = 11.84YY126 pKa = 9.0EE127 pKa = 4.14EE128 pKa = 4.23EE129 pKa = 4.61TICSSINGITDD140 pKa = 3.14ATVYY144 pKa = 10.59ISDD147 pKa = 4.46ALHH150 pKa = 5.92FMKK153 pKa = 10.52PSFVLGLFAGNPSIQQVYY171 pKa = 7.99ATLVLPPEE179 pKa = 4.73ALHH182 pKa = 5.95KK183 pKa = 10.62HH184 pKa = 6.0PSAHH188 pKa = 6.39PNLYY192 pKa = 10.03NINYY196 pKa = 9.25NFGGFQYY203 pKa = 10.57IPGKK207 pKa = 9.59HH208 pKa = 6.09AGGAYY213 pKa = 9.41HH214 pKa = 7.22HH215 pKa = 6.47EE216 pKa = 4.51LEE218 pKa = 4.26TLEE221 pKa = 3.92WLKK224 pKa = 10.63IGKK227 pKa = 8.86IFYY230 pKa = 10.8NKK232 pKa = 10.26GLTDD236 pKa = 3.33QKK238 pKa = 11.32VITVQLLEE246 pKa = 4.25SLGANHH252 pKa = 7.35LFLFRR257 pKa = 11.84RR258 pKa = 11.84GDD260 pKa = 3.19WVTPRR265 pKa = 11.84VRR267 pKa = 11.84TFSLDD272 pKa = 3.34EE273 pKa = 4.32YY274 pKa = 9.96VTLPQIFHH282 pKa = 7.32PKK284 pKa = 8.85LANASRR290 pKa = 11.84PIRR293 pKa = 11.84FTLANQLLLYY303 pKa = 10.05VKK305 pKa = 10.03SIKK308 pKa = 9.6TATQRR313 pKa = 11.84DD314 pKa = 3.72IFAKK318 pKa = 10.2LRR320 pKa = 11.84QLIKK324 pKa = 10.18TEE326 pKa = 4.08DD327 pKa = 3.59LSRR330 pKa = 11.84YY331 pKa = 10.0APDD334 pKa = 3.59EE335 pKa = 4.0LVHH338 pKa = 6.15IANYY342 pKa = 9.98FLFVGEE348 pKa = 5.09LSAEE352 pKa = 3.92NCYY355 pKa = 11.44DD356 pKa = 3.61NLLKK360 pKa = 10.4MGFFRR365 pKa = 11.84KK366 pKa = 8.49CTVPIKK372 pKa = 10.75NAFLKK377 pKa = 10.47LWRR380 pKa = 11.84TLFGKK385 pKa = 10.8LEE387 pKa = 4.0FEE389 pKa = 4.46QLLQSLEE396 pKa = 4.24WKK398 pKa = 9.41PFSYY402 pKa = 10.89SLLVIDD408 pKa = 5.69RR409 pKa = 11.84EE410 pKa = 4.46YY411 pKa = 11.21NDD413 pKa = 3.05YY414 pKa = 9.37TVRR417 pKa = 11.84VEE419 pKa = 3.86ARR421 pKa = 11.84KK422 pKa = 9.53EE423 pKa = 3.95KK424 pKa = 9.54YY425 pKa = 9.8HH426 pKa = 6.43KK427 pKa = 10.69LNSQFHH433 pKa = 5.1STWAIEE439 pKa = 4.08DD440 pKa = 3.99TEE442 pKa = 5.07ADD444 pKa = 3.02IDD446 pKa = 4.18DD447 pKa = 4.67LVTRR451 pKa = 11.84MTTEE455 pKa = 3.47LHH457 pKa = 6.39IPLPLPSPNDD467 pKa = 3.69DD468 pKa = 3.63GQKK471 pKa = 10.84GNSEE475 pKa = 4.02EE476 pKa = 5.55AKK478 pKa = 8.45TQPEE482 pKa = 4.4APMPAAEE489 pKa = 5.27KK490 pKa = 10.93GPTDD494 pKa = 3.62DD495 pKa = 3.96TSSPFAGGEE504 pKa = 4.08EE505 pKa = 4.4EE506 pKa = 4.61PSNIADD512 pKa = 3.28DD513 pKa = 4.11HH514 pKa = 6.21EE515 pKa = 4.76RR516 pKa = 11.84VNSSCEE522 pKa = 3.62ASTSQNAPAHH532 pKa = 6.38PGTSKK537 pKa = 10.62EE538 pKa = 3.91RR539 pKa = 11.84TVRR542 pKa = 11.84NAYY545 pKa = 9.57FLHH548 pKa = 6.57AMRR551 pKa = 11.84KK552 pKa = 8.63AASDD556 pKa = 3.83PNVFTDD562 pKa = 3.82INDD565 pKa = 4.88AILGACEE572 pKa = 3.46KK573 pKa = 9.68MEE575 pKa = 4.59RR576 pKa = 11.84SGCVDD581 pKa = 3.74SPWSPWASILNACGFSGDD599 pKa = 4.44YY600 pKa = 9.9IQHH603 pKa = 5.78DD604 pKa = 3.42QRR606 pKa = 11.84YY607 pKa = 8.2NLIYY611 pKa = 9.8PIQDD615 pKa = 2.56IKK617 pKa = 11.35AVNQCPWPITVPTRR631 pKa = 11.84LAAALEE637 pKa = 4.3KK638 pKa = 9.83MGRR641 pKa = 11.84PATHH645 pKa = 6.77AKK647 pKa = 9.81IEE649 pKa = 4.19SRR651 pKa = 11.84RR652 pKa = 11.84AAAFASDD659 pKa = 3.98VKK661 pKa = 10.99NSRR664 pKa = 11.84TGMLLPKK671 pKa = 10.33QPIDD675 pKa = 3.13WKK677 pKa = 10.66QAFASKK683 pKa = 10.71CDD685 pKa = 3.67FADD688 pKa = 3.59TKK690 pKa = 11.13NFPCVVIHH698 pKa = 6.43GAGGSGKK705 pKa = 10.18SRR707 pKa = 11.84LIQNFLKK714 pKa = 10.1TVDD717 pKa = 2.99RR718 pKa = 11.84RR719 pKa = 11.84EE720 pKa = 3.77RR721 pKa = 11.84RR722 pKa = 11.84FCVVTPTLEE731 pKa = 5.14LRR733 pKa = 11.84NDD735 pKa = 3.43WLKK738 pKa = 10.86KK739 pKa = 10.02VPQLPLEE746 pKa = 4.2VFKK749 pKa = 10.7TFEE752 pKa = 3.86KK753 pKa = 11.12AMVQGSASIVIMDD766 pKa = 5.78DD767 pKa = 3.55YY768 pKa = 11.37TKK770 pKa = 11.07LPAGYY775 pKa = 8.87IEE777 pKa = 4.9AYY779 pKa = 9.76IMQHH783 pKa = 6.28PNVDD787 pKa = 4.22LLILTGDD794 pKa = 4.29PKK796 pKa = 10.75QSSHH800 pKa = 7.13HH801 pKa = 5.72EE802 pKa = 4.18QNSQAQISHH811 pKa = 7.34LEE813 pKa = 3.82PAHH816 pKa = 6.2TIFGQYY822 pKa = 9.31AKK824 pKa = 10.66YY825 pKa = 10.26YY826 pKa = 10.33INATHH831 pKa = 6.97RR832 pKa = 11.84NCSEE836 pKa = 3.83LANPLGVYY844 pKa = 9.89SEE846 pKa = 4.49NFLPLKK852 pKa = 10.24LALSSQVSDD861 pKa = 4.16GPILVPSQLKK871 pKa = 10.51SEE873 pKa = 4.43ALHH876 pKa = 5.26EE877 pKa = 4.76LGRR880 pKa = 11.84KK881 pKa = 8.81AYY883 pKa = 9.15TYY885 pKa = 10.73AGCQGLTTPSIQIMLDD901 pKa = 3.22QNTPLCSNEE910 pKa = 4.16VIYY913 pKa = 9.14TALSRR918 pKa = 11.84AVDD921 pKa = 4.52EE922 pKa = 4.22ITFINTGSNSTDD934 pKa = 3.51FWRR937 pKa = 11.84KK938 pKa = 8.26MDD940 pKa = 3.63CTPYY944 pKa = 11.05LKK946 pKa = 9.89TFLQAVRR953 pKa = 11.84EE954 pKa = 4.18PKK956 pKa = 10.67LPTEE960 pKa = 4.27KK961 pKa = 11.03CEE963 pKa = 4.17DD964 pKa = 3.73PQPRR968 pKa = 11.84EE969 pKa = 4.23PIIKK973 pKa = 8.3THH975 pKa = 6.53FPVEE979 pKa = 3.97NEE981 pKa = 3.67KK982 pKa = 11.26VIFEE986 pKa = 4.29KK987 pKa = 10.57EE988 pKa = 3.57ISEE991 pKa = 4.19LQDD994 pKa = 2.93KK995 pKa = 10.09FSRR998 pKa = 11.84EE999 pKa = 4.04LHH1001 pKa = 5.73TPAHH1005 pKa = 5.51GHH1007 pKa = 5.77SNAIQTEE1014 pKa = 4.19DD1015 pKa = 3.83TIVQLFQHH1023 pKa = 5.59QQAKK1027 pKa = 10.42DD1028 pKa = 3.36QTLYY1032 pKa = 10.05WATIDD1037 pKa = 4.94KK1038 pKa = 10.24RR1039 pKa = 11.84IKK1041 pKa = 10.56LATEE1045 pKa = 3.61EE1046 pKa = 4.52ANLKK1050 pKa = 10.42EE1051 pKa = 4.28FVFKK1055 pKa = 10.94KK1056 pKa = 10.65DD1057 pKa = 2.8IGDD1060 pKa = 3.78ILFLNYY1066 pKa = 9.51KK1067 pKa = 9.85EE1068 pKa = 5.27AMNLPNQPIPFDD1080 pKa = 3.5LQLYY1084 pKa = 6.82EE1085 pKa = 4.06NCKK1088 pKa = 10.54SEE1090 pKa = 4.33VQNTYY1095 pKa = 9.92LKK1097 pKa = 10.8KK1098 pKa = 10.48PISQLMNGEE1107 pKa = 4.55LRR1109 pKa = 11.84QSPDD1113 pKa = 3.29FHH1115 pKa = 6.66PHH1117 pKa = 7.33AIQLFLKK1124 pKa = 9.31SQWVKK1129 pKa = 11.17KK1130 pKa = 9.94NDD1132 pKa = 4.39CIGLLQVKK1140 pKa = 9.75AGQTIASFMQQTVMIFGTMARR1161 pKa = 11.84YY1162 pKa = 7.4MRR1164 pKa = 11.84RR1165 pKa = 11.84MRR1167 pKa = 11.84QRR1169 pKa = 11.84YY1170 pKa = 5.77QPRR1173 pKa = 11.84KK1174 pKa = 9.91IMINCEE1180 pKa = 4.05TTPEE1184 pKa = 4.94DD1185 pKa = 2.88ISKK1188 pKa = 10.23FLKK1191 pKa = 10.49QEE1193 pKa = 3.94WNFKK1197 pKa = 10.12RR1198 pKa = 11.84PAYY1201 pKa = 10.49ANDD1204 pKa = 3.24YY1205 pKa = 10.91SAFDD1209 pKa = 3.64QSQDD1213 pKa = 2.94GAMLQFEE1220 pKa = 4.74VIKK1223 pKa = 10.67AKK1225 pKa = 8.22YY1226 pKa = 8.65HH1227 pKa = 6.39CIPEE1231 pKa = 4.4EE1232 pKa = 5.13IIDD1235 pKa = 3.85AYY1237 pKa = 10.06IAIKK1241 pKa = 10.66LNAKK1245 pKa = 9.71IFLGTLAIMRR1255 pKa = 11.84LTGEE1259 pKa = 4.65GPTFDD1264 pKa = 6.01ANTEE1268 pKa = 4.14CNIAYY1273 pKa = 9.91HH1274 pKa = 5.83FARR1277 pKa = 11.84FNMPPDD1283 pKa = 3.44TAYY1286 pKa = 11.19LFAGDD1291 pKa = 5.95DD1292 pKa = 3.84MAQDD1296 pKa = 3.59APVEE1300 pKa = 4.39EE1301 pKa = 4.67KK1302 pKa = 11.12SSFAKK1307 pKa = 10.41IKK1309 pKa = 10.64DD1310 pKa = 3.74RR1311 pKa = 11.84LSLTSKK1317 pKa = 10.48PLTFKK1322 pKa = 10.7QGPGDD1327 pKa = 3.61YY1328 pKa = 10.99ASFCGSLITPLGLLKK1343 pKa = 10.24EE1344 pKa = 4.18ARR1346 pKa = 11.84KK1347 pKa = 9.02LAASLEE1353 pKa = 4.23LANRR1357 pKa = 11.84TGNLKK1362 pKa = 10.11NVAASYY1368 pKa = 10.47AQDD1371 pKa = 3.17IFPTFVQGDD1380 pKa = 4.45RR1381 pKa = 11.84IYY1383 pKa = 10.86DD1384 pKa = 3.21IFTPEE1389 pKa = 4.06EE1390 pKa = 3.99QTHH1393 pKa = 5.96HH1394 pKa = 6.46RR1395 pKa = 11.84NSVRR1399 pKa = 11.84FLVKK1403 pKa = 10.11SAHH1406 pKa = 5.01QNFGEE1411 pKa = 4.6LVAQKK1416 pKa = 10.64LAGDD1420 pKa = 4.14SDD1422 pKa = 4.7SDD1424 pKa = 5.15DD1425 pKa = 4.14EE1426 pKa = 4.47DD1427 pKa = 3.96TGDD1430 pKa = 4.26SLMKK1434 pKa = 10.44HH1435 pKa = 6.35LSS1437 pKa = 3.28

Molecular weight:
162.63 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A220NQ63|A0A220NQ63_9VIRU Movement protein TGBp3 OS=Vanilla virus X OX=2016427 GN=TGBp3 PE=3 SV=1
MM1 pKa = 7.6PLIHH5 pKa = 7.15PPDD8 pKa = 3.6HH9 pKa = 6.51TNAIRR14 pKa = 11.84IAAVFLGVTLTIYY27 pKa = 11.01ALRR30 pKa = 11.84VNTLPHH36 pKa = 6.52VGDD39 pKa = 4.69NIHH42 pKa = 6.79ALPHH46 pKa = 5.85GGRR49 pKa = 11.84YY50 pKa = 8.24QDD52 pKa = 3.33GTKK55 pKa = 10.07TIFYY59 pKa = 9.27NSPFRR64 pKa = 11.84SQVPSTLPAILALILPAIIYY84 pKa = 9.86ALSRR88 pKa = 11.84HH89 pKa = 5.39HH90 pKa = 6.48RR91 pKa = 11.84TRR93 pKa = 11.84VVTAWADD100 pKa = 3.59THH102 pKa = 6.39HH103 pKa = 6.72HH104 pKa = 5.93HH105 pKa = 6.78HH106 pKa = 6.9HH107 pKa = 6.66PAA109 pKa = 3.89

Molecular weight:
12.15 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2052

62

1437

410.4

45.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.064 ± 1.373

1.559 ± 0.194

5.458 ± 0.491

5.068 ± 1.467

4.581 ± 0.702

4.873 ± 0.424

3.46 ± 1.25

6.823 ± 1.092

5.653 ± 1.333

9.698 ± 1.147

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.657 ± 0.351

4.63 ± 0.559

6.287 ± 0.693

4.678 ± 0.516

4.678 ± 0.322

5.75 ± 0.637

7.115 ± 1.384

4.922 ± 1.021

0.975 ± 0.141

3.07 ± 0.665

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski