Streptococcus phage Javan174

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6ASQ7|A0A4D6ASQ7_9CAUD Uncharacterized protein OS=Streptococcus phage Javan174 OX=2548023 GN=Javan174_0023 PE=4 SV=1
MM1 pKa = 7.1AQDD4 pKa = 3.37RR5 pKa = 11.84GLFGTFDD12 pKa = 4.06YY13 pKa = 8.87DD14 pKa = 2.8TWLATEE20 pKa = 4.72PDD22 pKa = 4.03YY23 pKa = 11.53CPHH26 pKa = 6.61EE27 pKa = 4.2EE28 pKa = 5.37SYY30 pKa = 11.63DD31 pKa = 3.55EE32 pKa = 5.29DD33 pKa = 3.45EE34 pKa = 6.29AYY36 pKa = 10.58DD37 pKa = 3.54RR38 pKa = 11.84WVDD41 pKa = 3.54AQSKK45 pKa = 9.86HH46 pKa = 5.07LAEE49 pKa = 4.53RR50 pKa = 11.84LL51 pKa = 3.52

Molecular weight: 6.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AS11|A0A4D6AS11_9CAUD Minor capsid protein OS=Streptococcus phage Javan174 OX=2548023 GN=Javan174_0045 PE=4 SV=1
MM1 pKa = 7.69GGRR4 pKa = 11.84GAKK7 pKa = 9.34IGRR10 pKa = 11.84LVEE13 pKa = 4.19VKK15 pKa = 10.62DD16 pKa = 3.66NRR18 pKa = 11.84GKK20 pKa = 10.23ILSTYY25 pKa = 10.16RR26 pKa = 11.84KK27 pKa = 8.83GKK29 pKa = 10.5GGIAEE34 pKa = 4.64KK35 pKa = 10.61LNPSNGVWIPVEE47 pKa = 3.65TATWKK52 pKa = 10.99NILKK56 pKa = 9.74KK57 pKa = 10.74AKK59 pKa = 10.33ARR61 pKa = 11.84GHH63 pKa = 6.99DD64 pKa = 3.22IRR66 pKa = 6.35

Molecular weight: 7.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

11623

40

1094

197.0

21.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.208 ± 0.876

0.404 ± 0.074

6.461 ± 0.37

6.367 ± 0.481

3.872 ± 0.225

7.244 ± 0.53

1.54 ± 0.172

6.238 ± 0.208

7.304 ± 0.422

7.95 ± 0.412

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.34 ± 0.195

5.412 ± 0.371

2.788 ± 0.208

4.061 ± 0.261

4.087 ± 0.296

6.685 ± 0.53

6.728 ± 0.43

6.952 ± 0.335

1.291 ± 0.129

4.07 ± 0.363
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski