Rhodobacteraceae bacterium HTCC2083

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; unclassified Rhodobacteraceae

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4108 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B6AVR7|B6AVR7_9RHOB Probable transcriptional regulator protein AraC family putative OS=Rhodobacteraceae bacterium HTCC2083 OX=314270 GN=RB2083_1284 PE=4 SV=1
MM1 pKa = 7.34TNATPLEE8 pKa = 4.38GTPLIVPSSTDD19 pKa = 2.94HH20 pKa = 6.76PLYY23 pKa = 10.5EE24 pKa = 4.63GLVEE28 pKa = 4.4ACRR31 pKa = 11.84TVYY34 pKa = 10.74DD35 pKa = 3.74PEE37 pKa = 5.17IPVNIYY43 pKa = 10.8DD44 pKa = 4.42LGLIYY49 pKa = 10.34TIDD52 pKa = 3.6VNEE55 pKa = 4.16EE56 pKa = 3.71NDD58 pKa = 3.76VKK60 pKa = 11.48VLMSLTAPGCPVAGEE75 pKa = 4.1MPGWVAEE82 pKa = 4.85AIEE85 pKa = 4.12PMAGVKK91 pKa = 8.56TVNVEE96 pKa = 4.4LVWEE100 pKa = 4.78PPWGMEE106 pKa = 3.89MMSDD110 pKa = 3.74EE111 pKa = 4.65ARR113 pKa = 11.84LEE115 pKa = 4.14LGFMM119 pKa = 4.54

Molecular weight: 13.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B6B192|B6B192_9RHOB DNA topoisomerase 1 OS=Rhodobacteraceae bacterium HTCC2083 OX=314270 GN=topA PE=3 SV=1
MM1 pKa = 7.16PTNSGSARR9 pKa = 11.84GAKK12 pKa = 7.71VAKK15 pKa = 9.27MGRR18 pKa = 11.84NLAILLKK25 pKa = 10.35RR26 pKa = 11.84LRR28 pKa = 11.84QLARR32 pKa = 11.84AVPSGG37 pKa = 3.22

Molecular weight: 3.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4108

0

4108

1152315

30

2137

280.5

30.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.275 ± 0.04

0.977 ± 0.014

5.981 ± 0.035

5.872 ± 0.037

3.993 ± 0.029

8.29 ± 0.04

2.13 ± 0.022

5.76 ± 0.029

4.007 ± 0.031

9.758 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.03 ± 0.022

3.145 ± 0.023

4.584 ± 0.029

3.313 ± 0.021

5.735 ± 0.036

5.768 ± 0.03

5.589 ± 0.025

7.107 ± 0.032

1.346 ± 0.015

2.336 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski