Actinoplanes friuliensis DSM 7358

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Actinoplanes; Actinoplanes friuliensis

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8558 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5VU47|U5VU47_9ACTN Putative MFS transporter OS=Actinoplanes friuliensis DSM 7358 OX=1246995 GN=AFR_04285 PE=4 SV=1
MM1 pKa = 8.25DD2 pKa = 6.16DD3 pKa = 3.3PTAFLTEE10 pKa = 3.98PAKK13 pKa = 10.78PDD15 pKa = 3.58DD16 pKa = 3.99SVSNGFANPLDD27 pKa = 3.7LFNYY31 pKa = 8.8VSPSAWLNAAIEE43 pKa = 4.32KK44 pKa = 9.13LTGVDD49 pKa = 3.24VFGWMTDD56 pKa = 3.01WVSGDD61 pKa = 3.39WEE63 pKa = 4.61SLWKK67 pKa = 11.14YY68 pKa = 9.82GDD70 pKa = 3.32AMANLAQCMQQIGINIQTGMLQLDD94 pKa = 4.05ASWDD98 pKa = 3.87GNASDD103 pKa = 4.66AAYY106 pKa = 9.66KK107 pKa = 10.65YY108 pKa = 10.94FSDD111 pKa = 4.55LAAATSGQQVAIAKK125 pKa = 7.75TQDD128 pKa = 3.56SYY130 pKa = 12.12HH131 pKa = 6.45KK132 pKa = 9.98AALGAWQLSNQLGNILQALADD153 pKa = 3.87KK154 pKa = 10.92AILAGIAAAAGTALIEE170 pKa = 4.34TGVGAVAGYY179 pKa = 9.43GAAAYY184 pKa = 9.25IVLDD188 pKa = 3.8MLQLINDD195 pKa = 3.94ASVIINTAGTVILGLFGGVMDD216 pKa = 5.47AAYY219 pKa = 10.42QGGDD223 pKa = 3.42LTAVPLPATAYY234 pKa = 9.99SGPGAA239 pKa = 4.07

Molecular weight: 24.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5W634|U5W634_9ACTN YceI domain-containing protein OS=Actinoplanes friuliensis DSM 7358 OX=1246995 GN=AFR_31890 PE=4 SV=1
MM1 pKa = 7.07TRR3 pKa = 11.84RR4 pKa = 11.84GGISRR9 pKa = 11.84IRR11 pKa = 11.84NGHH14 pKa = 5.22TGGPATGRR22 pKa = 11.84PDD24 pKa = 3.45PRR26 pKa = 11.84LTAIDD31 pKa = 3.55RR32 pKa = 11.84RR33 pKa = 11.84MPASGLLRR41 pKa = 11.84DD42 pKa = 3.76TTPPRR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84LTRR52 pKa = 11.84RR53 pKa = 11.84SLSGHH58 pKa = 5.81GLTRR62 pKa = 11.84HH63 pKa = 5.37RR64 pKa = 11.84TTSRR68 pKa = 11.84RR69 pKa = 11.84LTRR72 pKa = 11.84HH73 pKa = 5.27GLTWHH78 pKa = 6.18WLARR82 pKa = 11.84HH83 pKa = 6.21RR84 pKa = 11.84LPGNGLARR92 pKa = 11.84SRR94 pKa = 11.84TTRR97 pKa = 11.84HH98 pKa = 5.64GLTLTGTAGRR108 pKa = 11.84GLTRR112 pKa = 11.84RR113 pKa = 11.84RR114 pKa = 11.84VTGPGLTRR122 pKa = 11.84TGTTRR127 pKa = 11.84HH128 pKa = 5.82SLTSRR133 pKa = 11.84SMTSPRR139 pKa = 11.84LTRR142 pKa = 11.84TWTTRR147 pKa = 11.84HH148 pKa = 5.84GLTSSSMTGGGLTRR162 pKa = 11.84TRR164 pKa = 11.84TTRR167 pKa = 11.84HH168 pKa = 5.48GLTSRR173 pKa = 11.84SMTGRR178 pKa = 11.84GLTRR182 pKa = 11.84NGTTRR187 pKa = 11.84NGLTSRR193 pKa = 11.84SLTRR197 pKa = 11.84PRR199 pKa = 11.84LTRR202 pKa = 11.84TWTTRR207 pKa = 11.84HH208 pKa = 5.38GLTRR212 pKa = 11.84SGLTLNRR219 pKa = 11.84TGTAGRR225 pKa = 11.84GLAGRR230 pKa = 11.84RR231 pKa = 11.84VTGRR235 pKa = 11.84GLTRR239 pKa = 11.84ARR241 pKa = 11.84TTRR244 pKa = 11.84HH245 pKa = 5.49SLTSRR250 pKa = 11.84SMTT253 pKa = 3.47

Molecular weight: 27.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8558

0

8558

2802956

29

6661

327.5

34.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.646 ± 0.038

0.659 ± 0.007

6.074 ± 0.023

5.094 ± 0.026

2.836 ± 0.015

9.262 ± 0.029

2.028 ± 0.012

3.608 ± 0.019

2.004 ± 0.022

10.532 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.685 ± 0.009

1.981 ± 0.019

5.983 ± 0.024

2.847 ± 0.018

7.659 ± 0.031

5.194 ± 0.023

6.488 ± 0.032

8.784 ± 0.025

1.568 ± 0.012

2.067 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski