Nocardia sp. NRRL S-836

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Nocardia; unclassified Nocardia

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7876 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M8WA29|A0A0M8WA29_9NOCA Lysine 2 3-aminomutase OS=Nocardia sp. NRRL S-836 OX=1519492 GN=ADL03_27485 PE=4 SV=1
MM1 pKa = 7.21SHH3 pKa = 5.76NTATSGDD10 pKa = 3.93SGDD13 pKa = 3.97SVDD16 pKa = 5.36WNDD19 pKa = 5.84DD20 pKa = 3.35DD21 pKa = 6.01WNDD24 pKa = 5.1NDD26 pKa = 5.58NDD28 pKa = 4.12WDD30 pKa = 5.08DD31 pKa = 4.88DD32 pKa = 4.72DD33 pKa = 7.05SDD35 pKa = 4.35GGPTGDD41 pKa = 3.82TGDD44 pKa = 4.87AINVSSTNGGNTGHH58 pKa = 6.98GGNGGDD64 pKa = 4.32ADD66 pKa = 4.12ATSEE70 pKa = 4.27SHH72 pKa = 5.3VAVYY76 pKa = 10.63EE77 pKa = 5.09DD78 pKa = 6.1DD79 pKa = 6.44DD80 pKa = 7.4DD81 pKa = 7.66DD82 pKa = 7.76DD83 pKa = 5.38DD84 pKa = 6.25DD85 pKa = 6.63KK86 pKa = 11.99DD87 pKa = 3.78GAHH90 pKa = 5.18QQEE93 pKa = 5.06GAHH96 pKa = 5.24QQDD99 pKa = 4.23GVHH102 pKa = 6.1HH103 pKa = 6.53QDD105 pKa = 3.56GAHH108 pKa = 6.09HH109 pKa = 6.47QDD111 pKa = 3.88GVHH114 pKa = 6.51HH115 pKa = 6.83GSDD118 pKa = 3.19ATAVAVGGNGAPSGEE133 pKa = 4.12GGNALSHH140 pKa = 5.65NTATSGEE147 pKa = 4.35SGDD150 pKa = 4.46SGDD153 pKa = 5.49SGDD156 pKa = 4.07TGHH159 pKa = 6.74NSAIVMCVKK168 pKa = 9.49ATCLTGLWSGHH179 pKa = 6.24SGDD182 pKa = 3.95TSTGNTGDD190 pKa = 3.62AVNVSSTNGGNTGNGGNGGNADD212 pKa = 3.95ADD214 pKa = 4.16ANTNGHH220 pKa = 4.83GHH222 pKa = 6.0ATAVAAAGHH231 pKa = 6.38GKK233 pKa = 9.79PSGEE237 pKa = 3.99GGNALSHH244 pKa = 6.41NSATSGEE251 pKa = 4.43SGDD254 pKa = 4.46SGDD257 pKa = 5.49SGDD260 pKa = 4.07TGHH263 pKa = 6.74NSAIVMCVQADD274 pKa = 3.83CSTGVWSGASGDD286 pKa = 3.8TGTGNTGDD294 pKa = 4.12AVNEE298 pKa = 4.23STTTGGNTGHH308 pKa = 7.02GGNGGNADD316 pKa = 3.21AWATSWHH323 pKa = 6.43EE324 pKa = 3.96GDD326 pKa = 4.45HH327 pKa = 5.77NN328 pKa = 3.92

Molecular weight: 31.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N0A4X6|A0A0N0A4X6_9NOCA Peptidyl-tRNA hydrolase OS=Nocardia sp. NRRL S-836 OX=1519492 GN=pth PE=3 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.53GKK5 pKa = 8.66RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84AKK17 pKa = 8.7THH19 pKa = 5.15GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AILAARR37 pKa = 11.84RR38 pKa = 11.84SKK40 pKa = 10.82GRR42 pKa = 11.84ATLSAA47 pKa = 4.08

Molecular weight: 5.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7876

0

7876

2515523

27

11006

319.4

34.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.965 ± 0.043

0.799 ± 0.008

6.041 ± 0.025

5.554 ± 0.027

2.94 ± 0.017

8.973 ± 0.031

2.371 ± 0.015

3.16 ± 0.019

2.171 ± 0.023

10.507 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.706 ± 0.012

2.056 ± 0.019

5.678 ± 0.025

2.939 ± 0.016

7.834 ± 0.029

5.183 ± 0.022

6.088 ± 0.029

9.481 ± 0.03

1.59 ± 0.013

1.964 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski