La Piedad-Michoacan-Mexico virus (LPMV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Paramyxoviridae; Rubulavirinae; Orthorubulavirus

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q917N9|Q917N9_LPMV Isoform of A7E3E6 Non-structural protein V OS=La Piedad-Michoacan-Mexico virus OX=2560645 GN=P PE=2 SV=1
MM1 pKa = 7.66ASSSLSFSDD10 pKa = 5.1GEE12 pKa = 4.06ITEE15 pKa = 4.31LLEE18 pKa = 4.49TGLGTIEE25 pKa = 4.28SIEE28 pKa = 4.23RR29 pKa = 11.84MVAAKK34 pKa = 10.08GGPDD38 pKa = 3.34GGIDD42 pKa = 3.71PEE44 pKa = 4.42SQPGQRR50 pKa = 11.84GLPTPTPRR58 pKa = 11.84TTSTPTAAGSASATLEE74 pKa = 3.85LSPEE78 pKa = 3.82GGAIKK83 pKa = 10.15KK84 pKa = 10.24APRR87 pKa = 11.84AHH89 pKa = 6.38PTLPNPLGQEE99 pKa = 3.99EE100 pKa = 4.85RR101 pKa = 11.84PGNPLSTFTPVRR113 pKa = 11.84GSSSTHH119 pKa = 6.64DD120 pKa = 4.3PLPGSRR126 pKa = 11.84PGSEE130 pKa = 4.33VYY132 pKa = 10.54EE133 pKa = 4.62GDD135 pKa = 3.06LMARR139 pKa = 11.84ARR141 pKa = 11.84SEE143 pKa = 4.39LVTRR147 pKa = 11.84WSDD150 pKa = 3.51EE151 pKa = 3.99EE152 pKa = 4.53GDD154 pKa = 3.95PVPTRR159 pKa = 11.84VLQSTFKK166 pKa = 10.66RR167 pKa = 11.84GADD170 pKa = 2.8RR171 pKa = 11.84KK172 pKa = 9.75GANPGEE178 pKa = 4.35PGHH181 pKa = 6.15RR182 pKa = 11.84RR183 pKa = 11.84EE184 pKa = 4.3YY185 pKa = 10.72SIGWVCGTVRR195 pKa = 11.84VLEE198 pKa = 4.31WCNPACSPISMEE210 pKa = 3.56PRR212 pKa = 11.84YY213 pKa = 9.01YY214 pKa = 10.51QCTCGTCPAKK224 pKa = 10.6CPQCAGDD231 pKa = 3.73NGIVEE236 pKa = 4.94SNRR239 pKa = 11.84GPHH242 pKa = 6.94DD243 pKa = 3.62GAGNEE248 pKa = 4.29SS249 pKa = 3.43

Molecular weight: 26.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q917N9|Q917N9_LPMV Isoform of A7E3E6 Non-structural protein V OS=La Piedad-Michoacan-Mexico virus OX=2560645 GN=P PE=2 SV=1
MM1 pKa = 7.48EE2 pKa = 5.94RR3 pKa = 11.84LLNYY7 pKa = 8.15WKK9 pKa = 10.18QDD11 pKa = 2.96WALLRR16 pKa = 11.84VSRR19 pKa = 11.84GWWRR23 pKa = 11.84QRR25 pKa = 11.84VGLTGEE31 pKa = 4.68LTQSPNLDD39 pKa = 3.13RR40 pKa = 11.84GDD42 pKa = 3.69FRR44 pKa = 11.84LPPPGQHH51 pKa = 5.01QHH53 pKa = 6.75PPLQEE58 pKa = 3.91VPLLLLSLVRR68 pKa = 11.84KK69 pKa = 9.39EE70 pKa = 4.29EE71 pKa = 3.69QSKK74 pKa = 9.44RR75 pKa = 11.84HH76 pKa = 6.01LGRR79 pKa = 11.84TLHH82 pKa = 6.65SPTPLGKK89 pKa = 10.32KK90 pKa = 9.4NALEE94 pKa = 4.09TPYY97 pKa = 10.55PHH99 pKa = 7.2LPQSEE104 pKa = 4.47VHH106 pKa = 6.45HH107 pKa = 6.65PLMIPSLEE115 pKa = 4.1VVLDD119 pKa = 3.71QKK121 pKa = 10.84FMRR124 pKa = 11.84AII126 pKa = 3.65

Molecular weight: 14.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

3

9

5235

126

2251

581.7

64.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.813 ± 0.976

1.796 ± 0.307

4.394 ± 0.462

5.444 ± 0.54

3.362 ± 0.481

6.399 ± 0.933

2.006 ± 0.349

6.38 ± 0.543

3.572 ± 0.527

10.869 ± 0.914

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.541 ± 0.178

3.859 ± 0.352

6.418 ± 1.192

4.852 ± 0.299

5.788 ± 0.442

7.335 ± 0.77

6.972 ± 0.784

5.826 ± 0.442

1.203 ± 0.173

3.171 ± 0.442
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski