Mona Grita virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Phlebovirus; Mona Grita phlebovirus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482KEM8|A0A482KEM8_9VIRU Nonstructural protein OS=Mona Grita virus OX=2559111 GN=NSs PE=4 SV=1
MM1 pKa = 7.36NFLYY5 pKa = 10.04DD6 pKa = 3.34QPVIFRR12 pKa = 11.84MGGVGDD18 pKa = 4.12PEE20 pKa = 4.55VEE22 pKa = 3.97VGYY25 pKa = 10.77LPFEE29 pKa = 4.11AWSVRR34 pKa = 11.84KK35 pKa = 9.71ICVHH39 pKa = 5.11NTMEE43 pKa = 4.95IPLEE47 pKa = 4.19TYY49 pKa = 10.13RR50 pKa = 11.84FAMDD54 pKa = 3.55HH55 pKa = 6.68RR56 pKa = 11.84PTLRR60 pKa = 11.84DD61 pKa = 3.82FYY63 pKa = 11.53SRR65 pKa = 11.84GEE67 pKa = 3.99FPFRR71 pKa = 11.84WGPGTFNSRR80 pKa = 11.84VQEE83 pKa = 4.02EE84 pKa = 4.73TTNSFDD90 pKa = 3.68SAIMDD95 pKa = 3.83LVGFPLASFTKK106 pKa = 10.53SFLPNVKK113 pKa = 9.96LALSWPLGYY122 pKa = 7.71PTTDD126 pKa = 4.54FIQLSGIPGHH136 pKa = 5.71FTHH139 pKa = 6.76NGWKK143 pKa = 10.31GMAATSLFRR152 pKa = 11.84MNPQLEE158 pKa = 4.32RR159 pKa = 11.84FDD161 pKa = 3.76QVFVEE166 pKa = 3.99AHH168 pKa = 5.69KK169 pKa = 11.07SILEE173 pKa = 3.86EE174 pKa = 3.99AEE176 pKa = 3.99RR177 pKa = 11.84RR178 pKa = 11.84GLSSDD183 pKa = 3.52YY184 pKa = 10.08FTGYY188 pKa = 11.12DD189 pKa = 3.61LLKK192 pKa = 10.51EE193 pKa = 4.27VASLQIVRR201 pKa = 11.84LLNAVDD207 pKa = 3.34IDD209 pKa = 4.06VVYY212 pKa = 11.38SNIPTNLTYY221 pKa = 11.18KK222 pKa = 10.9LLDD225 pKa = 3.72LRR227 pKa = 11.84DD228 pKa = 3.76TFEE231 pKa = 4.4NEE233 pKa = 4.05SPEE236 pKa = 4.09LLGNRR241 pKa = 11.84FWIPSPDD248 pKa = 2.81TWFDD252 pKa = 3.35GLYY255 pKa = 10.3LQTDD259 pKa = 3.67SSFSSDD265 pKa = 2.77EE266 pKa = 4.09DD267 pKa = 3.51

Molecular weight: 30.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482KC00|A0A482KC00_9VIRU Glycoprotein OS=Mona Grita virus OX=2559111 GN=GPC PE=4 SV=1
MM1 pKa = 7.56TDD3 pKa = 3.19YY4 pKa = 11.74AEE6 pKa = 4.07IAIAFAGEE14 pKa = 4.34PINNAEE20 pKa = 3.85VMGWVNEE27 pKa = 3.96FAYY30 pKa = 10.14EE31 pKa = 4.14GFSAQRR37 pKa = 11.84IIQLVQEE44 pKa = 5.25RR45 pKa = 11.84GPQTWQVDD53 pKa = 3.9VKK55 pKa = 10.81MMIVLALTRR64 pKa = 11.84GNKK67 pKa = 7.49PSKK70 pKa = 10.02MIEE73 pKa = 3.94KK74 pKa = 9.86MSAEE78 pKa = 4.22GKK80 pKa = 10.19KK81 pKa = 10.14KK82 pKa = 9.12ATRR85 pKa = 11.84LITTYY90 pKa = 10.48NLKK93 pKa = 10.41SGNPGRR99 pKa = 11.84DD100 pKa = 3.45DD101 pKa = 3.54LTLSRR106 pKa = 11.84VASAFAGWTCQALAVLHH123 pKa = 6.86PYY125 pKa = 10.72LPVTGASMDD134 pKa = 4.42SISPNYY140 pKa = 8.29PRR142 pKa = 11.84AMMHH146 pKa = 6.7PSFAGLIDD154 pKa = 3.52NSIPEE159 pKa = 4.71AYY161 pKa = 9.95LQLVVDD167 pKa = 3.84AHH169 pKa = 6.94ALYY172 pKa = 10.84LLQFSRR178 pKa = 11.84VINKK182 pKa = 8.82NMRR185 pKa = 11.84GQPKK189 pKa = 9.72SVVVSSFLQPMNAAIVSGFISNDD212 pKa = 2.71KK213 pKa = 9.22RR214 pKa = 11.84RR215 pKa = 11.84KK216 pKa = 8.2MLMAFGIVDD225 pKa = 3.8QNGKK229 pKa = 7.34PTQAVEE235 pKa = 4.17TAAKK239 pKa = 10.82AFMTINN245 pKa = 3.28

Molecular weight: 26.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3947

245

2083

986.8

110.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.636 ± 1.085

2.584 ± 0.837

5.194 ± 0.562

6.587 ± 0.461

4.864 ± 0.573

6.486 ± 0.257

2.128 ± 0.176

6.714 ± 0.42

6.283 ± 0.659

9.121 ± 0.47

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.116 ± 0.408

4.18 ± 0.487

4.054 ± 0.478

3.344 ± 0.216

4.814 ± 0.278

10.312 ± 0.823

5.194 ± 0.191

6.309 ± 0.199

1.267 ± 0.165

2.812 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski