Proteus phage vB_PmiM_Pm5461

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; unclassified Tevenvirinae

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 256 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G2SSK2|A0A0G2SSK2_9CAUD Uncharacterized protein OS=Proteus phage vB_PmiM_Pm5461 OX=1636250 GN=Pm5461_125 PE=4 SV=1
MM1 pKa = 6.75KK2 pKa = 10.1TNVFMDD8 pKa = 4.24LFTSKK13 pKa = 10.94EE14 pKa = 4.16DD15 pKa = 3.19ICNEE19 pKa = 3.77FQIPTDD25 pKa = 3.49VLADD29 pKa = 3.7KK30 pKa = 10.56EE31 pKa = 4.41IVLAYY36 pKa = 9.59YY37 pKa = 10.46DD38 pKa = 3.99CEE40 pKa = 4.62DD41 pKa = 3.66YY42 pKa = 11.02CGCAIVIYY50 pKa = 10.72ADD52 pKa = 3.15ILGQFWEE59 pKa = 4.44VHH61 pKa = 5.86GSHH64 pKa = 6.94CSCYY68 pKa = 10.13GLEE71 pKa = 4.47GQWEE75 pKa = 4.3PEE77 pKa = 4.27LIGDD81 pKa = 4.13FEE83 pKa = 4.8LFKK86 pKa = 10.6EE87 pKa = 4.35YY88 pKa = 11.1SKK90 pKa = 9.26TQQIDD95 pKa = 3.36FGKK98 pKa = 10.49GSIYY102 pKa = 11.35NMIFF106 pKa = 2.96

Molecular weight: 12.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G2SSB0|A0A0G2SSB0_9CAUD Uncharacterized protein OS=Proteus phage vB_PmiM_Pm5461 OX=1636250 GN=Pm5461_203 PE=4 SV=1
MM1 pKa = 7.56SARR4 pKa = 11.84KK5 pKa = 9.45GYY7 pKa = 9.54KK8 pKa = 9.44RR9 pKa = 11.84KK10 pKa = 7.78TNKK13 pKa = 10.16RR14 pKa = 11.84INQDD18 pKa = 2.64LDD20 pKa = 3.54KK21 pKa = 11.31LVGFVKK27 pKa = 10.31RR28 pKa = 11.84QEE30 pKa = 3.69NGRR33 pKa = 11.84AKK35 pKa = 10.51FLANLGNKK43 pKa = 8.71KK44 pKa = 10.29

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

256

0

256

51479

33

1218

201.1

23.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.68 ± 0.144

1.197 ± 0.078

6.389 ± 0.105

7.56 ± 0.165

4.561 ± 0.106

5.645 ± 0.177

1.667 ± 0.057

8.131 ± 0.139

9.118 ± 0.163

8.168 ± 0.15

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.397 ± 0.071

6.035 ± 0.123

3.413 ± 0.085

3.085 ± 0.088

3.53 ± 0.106

7.053 ± 0.131

5.449 ± 0.169

6.026 ± 0.099

1.261 ± 0.056

4.635 ± 0.126
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski