Gordonia phage Ailee

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Vividuovirus; unclassified Vividuovirus

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3M7N9|A0A3G3M7N9_9CAUD Uncharacterized protein OS=Gordonia phage Ailee OX=2483665 GN=72 PE=4 SV=1
MM1 pKa = 7.6ASDD4 pKa = 5.73DD5 pKa = 3.67IRR7 pKa = 11.84NYY9 pKa = 11.28DD10 pKa = 3.81PDD12 pKa = 3.28TGMLYY17 pKa = 9.88RR18 pKa = 11.84WEE20 pKa = 4.57MPDD23 pKa = 3.1SPRR26 pKa = 11.84GFVVEE31 pKa = 5.22IEE33 pKa = 4.35NDD35 pKa = 3.4DD36 pKa = 5.08DD37 pKa = 5.74GPILCGCVTGPGFTKK52 pKa = 10.39PVGFLYY58 pKa = 9.98PAEE61 pKa = 4.24ARR63 pKa = 11.84WLAEE67 pKa = 3.93NMPSMLASIEE77 pKa = 4.22AYY79 pKa = 9.66EE80 pKa = 4.16LQQGATSS87 pKa = 3.39

Molecular weight: 9.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3M7M9|A0A3G3M7M9_9CAUD Uncharacterized protein OS=Gordonia phage Ailee OX=2483665 GN=61 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 10.12EE3 pKa = 3.92RR4 pKa = 11.84GKK6 pKa = 10.11HH7 pKa = 4.07RR8 pKa = 11.84PRR10 pKa = 11.84RR11 pKa = 11.84PRR13 pKa = 11.84VTVYY17 pKa = 10.12DD18 pKa = 4.07AQWRR22 pKa = 11.84AVWPQPNLDD31 pKa = 3.9LVEE34 pKa = 4.09VHH36 pKa = 6.76AWTNDD41 pKa = 2.81PVTEE45 pKa = 4.12VDD47 pKa = 3.21GHH49 pKa = 5.83LVIAARR55 pKa = 11.84VGWRR59 pKa = 11.84ARR61 pKa = 11.84IGDD64 pKa = 3.47VRR66 pKa = 11.84IQRR69 pKa = 11.84TEE71 pKa = 3.76TLL73 pKa = 3.42

Molecular weight: 8.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

18614

39

1604

224.3

24.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.088 ± 0.471

0.86 ± 0.141

7.548 ± 0.405

5.652 ± 0.337

2.503 ± 0.15

8.563 ± 0.471

2.24 ± 0.199

4.523 ± 0.255

2.557 ± 0.187

7.489 ± 0.277

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.063 ± 0.106

2.718 ± 0.171

6.463 ± 0.276

3.814 ± 0.221

7.306 ± 0.46

4.69 ± 0.222

6.624 ± 0.311

8.042 ± 0.252

2.095 ± 0.125

2.16 ± 0.142
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski