Circoviridae sp. ctfvP2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 7.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2W9F3|A0A5Q2W9F3_9CIRC ATP-dependent helicase Rep OS=Circoviridae sp. ctfvP2 OX=2656728 PE=3 SV=1
MM1 pKa = 7.44EE2 pKa = 5.1GKK4 pKa = 9.88SIKK7 pKa = 9.6RR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84YY11 pKa = 10.14LFTLNKK17 pKa = 9.56PLEE20 pKa = 4.53KK21 pKa = 10.04KK22 pKa = 10.7DD23 pKa = 3.19IDD25 pKa = 3.94FNNNVVRR32 pKa = 11.84CYY34 pKa = 10.34CYY36 pKa = 9.27QHH38 pKa = 6.41EE39 pKa = 4.47VGEE42 pKa = 4.69KK43 pKa = 9.95GTDD46 pKa = 2.78HH47 pKa = 5.66YY48 pKa = 11.33QGYY51 pKa = 9.51FEE53 pKa = 5.48LLRR56 pKa = 11.84PQHH59 pKa = 6.13LSWIKK64 pKa = 10.51RR65 pKa = 11.84NISNEE70 pKa = 3.8MHH72 pKa = 7.03VEE74 pKa = 3.83MCFGDD79 pKa = 3.89RR80 pKa = 11.84KK81 pKa = 11.2DD82 pKa = 3.77NVHH85 pKa = 6.1YY86 pKa = 10.11CSKK89 pKa = 10.41PHH91 pKa = 6.96GPDD94 pKa = 3.3DD95 pKa = 3.69EE96 pKa = 6.61CYY98 pKa = 10.45DD99 pKa = 3.91EE100 pKa = 6.11CGTICDD106 pKa = 4.68CKK108 pKa = 10.38NCKK111 pKa = 9.39KK112 pKa = 10.48ARR114 pKa = 11.84KK115 pKa = 8.94APQKK119 pKa = 10.38IKK121 pKa = 10.72DD122 pKa = 3.76IIFGGDD128 pKa = 2.75WDD130 pKa = 4.41LEE132 pKa = 4.5SGKK135 pKa = 8.94RR136 pKa = 11.84TDD138 pKa = 4.76LIEE141 pKa = 4.28FKK143 pKa = 10.77KK144 pKa = 10.85DD145 pKa = 2.95IVEE148 pKa = 4.19MSLKK152 pKa = 10.15EE153 pKa = 4.03LWEE156 pKa = 3.7KK157 pKa = 10.81HH158 pKa = 4.46YY159 pKa = 11.16FLMLRR164 pKa = 11.84YY165 pKa = 9.59RR166 pKa = 11.84NMVVHH171 pKa = 6.63MKK173 pKa = 10.76NDD175 pKa = 3.1INEE178 pKa = 4.22KK179 pKa = 9.32YY180 pKa = 10.23FVGIRR185 pKa = 11.84NVILIIGRR193 pKa = 11.84SGTGKK198 pKa = 10.03SEE200 pKa = 4.2MVNKK204 pKa = 9.25EE205 pKa = 3.37HH206 pKa = 7.23SKK208 pKa = 10.43IYY210 pKa = 10.56DD211 pKa = 3.0KK212 pKa = 11.34SLNVKK217 pKa = 10.48DD218 pKa = 3.27KK219 pKa = 11.06DD220 pKa = 3.5YY221 pKa = 10.63WEE223 pKa = 4.99KK224 pKa = 11.39YY225 pKa = 10.1DD226 pKa = 3.64GEE228 pKa = 4.33EE229 pKa = 3.95IIKK232 pKa = 10.63FEE234 pKa = 4.53DD235 pKa = 3.56FTGEE239 pKa = 3.88VEE241 pKa = 4.23MNEE244 pKa = 4.41MIRR247 pKa = 11.84ILDD250 pKa = 3.67RR251 pKa = 11.84YY252 pKa = 9.82KK253 pKa = 10.59VDD255 pKa = 2.81VCKK258 pKa = 10.27RR259 pKa = 11.84YY260 pKa = 9.94VGKK263 pKa = 8.5TPFNGKK269 pKa = 9.55VIYY272 pKa = 9.19ITTNTLPEE280 pKa = 3.71EE281 pKa = 4.43WYY283 pKa = 10.63KK284 pKa = 11.44NEE286 pKa = 4.2LNVEE290 pKa = 4.38RR291 pKa = 11.84FNALYY296 pKa = 10.38RR297 pKa = 11.84RR298 pKa = 11.84FTGYY302 pKa = 10.6VVTKK306 pKa = 9.87YY307 pKa = 10.58KK308 pKa = 10.46EE309 pKa = 4.05DD310 pKa = 3.7PVVSNCVNEE319 pKa = 4.34FKK321 pKa = 11.07DD322 pKa = 4.73DD323 pKa = 3.35ILSIMGEE330 pKa = 3.92EE331 pKa = 4.55KK332 pKa = 10.73YY333 pKa = 11.28NLFKK337 pKa = 10.9VYY339 pKa = 10.3KK340 pKa = 9.15CYY342 pKa = 11.06CHH344 pKa = 6.22MCNII348 pKa = 4.42

Molecular weight: 41.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2W9F3|A0A5Q2W9F3_9CIRC ATP-dependent helicase Rep OS=Circoviridae sp. ctfvP2 OX=2656728 PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 10.34NIYY5 pKa = 9.47IYY7 pKa = 10.33KK8 pKa = 10.02RR9 pKa = 11.84KK10 pKa = 9.77NFYY13 pKa = 10.83SISFKK18 pKa = 11.18NFLLLYY24 pKa = 10.16QDD26 pKa = 3.8GKK28 pKa = 10.1MVNPTCIYY36 pKa = 9.5CNKK39 pKa = 9.5VLLYY43 pKa = 9.76LHH45 pKa = 5.99IHH47 pKa = 6.06KK48 pKa = 10.37LRR50 pKa = 11.84RR51 pKa = 11.84QKK53 pKa = 10.94PNTCICGSLLFCCHH67 pKa = 7.19CDD69 pKa = 3.41CNIYY73 pKa = 10.02IYY75 pKa = 10.53CFKK78 pKa = 10.36CCNGRR83 pKa = 11.84KK84 pKa = 9.27INN86 pKa = 3.65

Molecular weight: 10.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

434

86

348

217.0

26.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

0.691 ± 0.34

5.76 ± 3.458

6.221 ± 1.916

7.373 ± 3.627

4.608 ± 0.593

5.3 ± 0.891

2.995 ± 0.242

7.834 ± 1.294

11.751 ± 0.061

6.682 ± 1.861

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.226 ± 0.443

7.604 ± 1.407

2.304 ± 0.011

1.382 ± 0.464

5.3 ± 0.319

3.456 ± 0.016

3.226 ± 0.443

5.991 ± 1.803

1.152 ± 0.567

7.143 ± 1.062
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski