Gordonia phage Kurt

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A386KIH4|A0A386KIH4_9CAUD HicA-like toxin OS=Gordonia phage Kurt OX=2315533 GN=9 PE=4 SV=1
MM1 pKa = 6.62THH3 pKa = 6.46SPAASDD9 pKa = 3.98VIATRR14 pKa = 11.84TLDD17 pKa = 3.7PVDD20 pKa = 4.51GFTLTAFEE28 pKa = 4.91VPDD31 pKa = 4.04TDD33 pKa = 3.76STPIGQSLDD42 pKa = 3.24EE43 pKa = 4.79GGCYY47 pKa = 8.92TADD50 pKa = 5.31DD51 pKa = 3.72IEE53 pKa = 4.04AWRR56 pKa = 11.84RR57 pKa = 11.84DD58 pKa = 2.76EE59 pKa = 3.88WQYY62 pKa = 11.21VGVVVVASRR71 pKa = 11.84AGVDD75 pKa = 3.79LGSASIWGMDD85 pKa = 3.27TGDD88 pKa = 3.48YY89 pKa = 10.09WDD91 pKa = 5.58AITRR95 pKa = 11.84ADD97 pKa = 3.04GRR99 pKa = 11.84TPYY102 pKa = 10.7GVLDD106 pKa = 3.78NVPNSVTTGHH116 pKa = 6.54PRR118 pKa = 11.84EE119 pKa = 4.96DD120 pKa = 3.3GGIDD124 pKa = 3.5YY125 pKa = 7.48VTRR128 pKa = 11.84DD129 pKa = 4.3DD130 pKa = 3.93FAHH133 pKa = 7.2GYY135 pKa = 9.33GADD138 pKa = 4.51LISEE142 pKa = 4.69ALTEE146 pKa = 4.09ARR148 pKa = 11.84ATVAALTSPDD158 pKa = 3.52VVIPVCDD165 pKa = 3.49EE166 pKa = 3.96CGEE169 pKa = 4.22RR170 pKa = 11.84ADD172 pKa = 4.52GVSDD176 pKa = 3.42AHH178 pKa = 6.9GVACSLFTGNVAGDD192 pKa = 4.17PYY194 pKa = 10.78EE195 pKa = 4.52VPAPTFYY202 pKa = 10.52PGAGGWHH209 pKa = 5.59TEE211 pKa = 3.81QRR213 pKa = 11.84GTCTYY218 pKa = 10.45HH219 pKa = 5.82VATIAGGHH227 pKa = 5.79RR228 pKa = 11.84AAYY231 pKa = 7.83VTLSDD236 pKa = 3.55ANEE239 pKa = 4.22YY240 pKa = 10.18VAGLYY245 pKa = 7.74DD246 pKa = 3.76TTDD249 pKa = 3.39TPHH252 pKa = 7.12VIGTFPTLFDD262 pKa = 3.78GKK264 pKa = 10.04RR265 pKa = 11.84AADD268 pKa = 3.53AARR271 pKa = 11.84EE272 pKa = 4.02RR273 pKa = 11.84GSVKK277 pKa = 10.58

Molecular weight: 29.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A386KIV6|A0A386KIV6_9CAUD Uncharacterized protein OS=Gordonia phage Kurt OX=2315533 GN=71 PE=4 SV=1
MM1 pKa = 7.42TIVNRR6 pKa = 11.84VPTLKK11 pKa = 10.38PRR13 pKa = 11.84GRR15 pKa = 11.84CGFVKK20 pKa = 9.67EE21 pKa = 4.1NKK23 pKa = 8.93MGVEE27 pKa = 4.33VRR29 pKa = 11.84CTLIAGHH36 pKa = 6.72DD37 pKa = 3.3EE38 pKa = 4.25GRR40 pKa = 11.84RR41 pKa = 11.84HH42 pKa = 6.41RR43 pKa = 11.84APDD46 pKa = 3.47SPSRR50 pKa = 11.84HH51 pKa = 5.56YY52 pKa = 7.37FTPLSAFDD60 pKa = 4.0EE61 pKa = 4.55NGVMVFNGRR70 pKa = 11.84IITAPKK76 pKa = 9.91DD77 pKa = 3.41LYY79 pKa = 10.57RR80 pKa = 11.84GYY82 pKa = 10.24IDD84 pKa = 3.34PFEE87 pKa = 4.17RR88 pKa = 11.84AKK90 pKa = 10.95ARR92 pKa = 11.84AAVYY96 pKa = 9.97DD97 pKa = 3.64QAARR101 pKa = 11.84AARR104 pKa = 11.84LSRR107 pKa = 11.84MRR109 pKa = 4.27

Molecular weight: 12.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

21856

40

1797

230.1

25.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.098 ± 0.453

0.837 ± 0.107

7.298 ± 0.294

6.483 ± 0.286

3.061 ± 0.125

8.625 ± 0.404

1.871 ± 0.147

4.941 ± 0.208

4.036 ± 0.269

7.426 ± 0.271

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.224 ± 0.104

3.088 ± 0.183

5.792 ± 0.212

3.157 ± 0.147

7.641 ± 0.394

5.312 ± 0.222

6.419 ± 0.251

7.119 ± 0.203

1.99 ± 0.118

2.581 ± 0.217
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski