Terrabacter sp. Root85

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Intrasporangiaceae; Terrabacter; unclassified Terrabacter

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4304 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q8YKF2|A0A0Q8YKF2_9MICO Uncharacterized protein OS=Terrabacter sp. Root85 OX=1736603 GN=ASE25_04195 PE=4 SV=1
MM1 pKa = 7.89SDD3 pKa = 3.85LDD5 pKa = 3.93YY6 pKa = 11.1PSDD9 pKa = 3.44LRR11 pKa = 11.84YY12 pKa = 8.66TSDD15 pKa = 3.69HH16 pKa = 5.31EE17 pKa = 4.37WVRR20 pKa = 11.84VGDD23 pKa = 4.08DD24 pKa = 3.44GVVRR28 pKa = 11.84IGITSFAQDD37 pKa = 3.07ALGDD41 pKa = 3.82VVYY44 pKa = 11.12VSLPAVGDD52 pKa = 3.74TVTPGDD58 pKa = 3.41SCGEE62 pKa = 4.13VEE64 pKa = 4.44STKK67 pKa = 10.76SVSDD71 pKa = 3.43VYY73 pKa = 11.43APLEE77 pKa = 4.37GEE79 pKa = 4.42VTATNAALDD88 pKa = 3.89SSPEE92 pKa = 4.43LINSDD97 pKa = 3.82PYY99 pKa = 10.96GEE101 pKa = 3.8GWMFEE106 pKa = 4.4LRR108 pKa = 11.84LADD111 pKa = 3.9PSAVEE116 pKa = 4.22GLMDD120 pKa = 5.05ASTYY124 pKa = 8.18TASLSS129 pKa = 3.56

Molecular weight: 13.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q8YG60|A0A0Q8YG60_9MICO Uncharacterized protein OS=Terrabacter sp. Root85 OX=1736603 GN=ASE25_09180 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.84GRR40 pKa = 11.84AEE42 pKa = 4.11LSAA45 pKa = 4.93

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4304

0

4304

1430963

29

3511

332.5

35.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.222 ± 0.05

0.669 ± 0.01

6.482 ± 0.032

5.309 ± 0.038

2.733 ± 0.02

9.309 ± 0.033

2.268 ± 0.018

3.403 ± 0.023

1.938 ± 0.029

10.0 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.778 ± 0.015

1.718 ± 0.022

5.692 ± 0.027

2.634 ± 0.018

7.594 ± 0.038

5.591 ± 0.026

6.496 ± 0.04

9.711 ± 0.036

1.585 ± 0.016

1.866 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski