Thalassococcus halodurans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Thalassococcus

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3829 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H6ATN9|A0A1H6ATN9_9RHOB 5-carboxymethyl-2-hydroxymuconate isomerase OS=Thalassococcus halodurans OX=373675 GN=SAMN04488045_3153 PE=4 SV=1
MM1 pKa = 7.28LRR3 pKa = 11.84ISAMILALALVSACGGGGSNPLQNTEE29 pKa = 4.17EE30 pKa = 4.53EE31 pKa = 4.4EE32 pKa = 4.27TDD34 pKa = 3.22TDD36 pKa = 3.57TGTDD40 pKa = 3.59TEE42 pKa = 5.24DD43 pKa = 3.81DD44 pKa = 3.99ADD46 pKa = 4.9PIGGDD51 pKa = 3.04RR52 pKa = 11.84TLPPGTASPTPDD64 pKa = 3.06TAIVRR69 pKa = 11.84VEE71 pKa = 3.92ADD73 pKa = 3.51DD74 pKa = 5.0GEE76 pKa = 4.73GAGVATGYY84 pKa = 10.87DD85 pKa = 3.78YY86 pKa = 11.61DD87 pKa = 3.94SATDD91 pKa = 3.36TFYY94 pKa = 11.66VDD96 pKa = 4.36NLAFDD101 pKa = 4.3GDD103 pKa = 3.84NTYY106 pKa = 11.15SRR108 pKa = 11.84GTAVSSLAPNIAVYY122 pKa = 10.42EE123 pKa = 4.07SDD125 pKa = 4.54APGIDD130 pKa = 4.37PISGLQHH137 pKa = 7.25DD138 pKa = 4.43DD139 pKa = 3.22AEE141 pKa = 4.31YY142 pKa = 9.09RR143 pKa = 11.84AVYY146 pKa = 9.74GVSDD150 pKa = 3.46TGTTKK155 pKa = 10.49FAIVRR160 pKa = 11.84PAGYY164 pKa = 10.21NDD166 pKa = 3.23FGFRR170 pKa = 11.84GYY172 pKa = 9.11TYY174 pKa = 10.16EE175 pKa = 4.36RR176 pKa = 11.84EE177 pKa = 4.4GSVTLPTDD185 pKa = 3.28IQATYY190 pKa = 9.4IGDD193 pKa = 3.61YY194 pKa = 10.57GGVRR198 pKa = 11.84VYY200 pKa = 10.87EE201 pKa = 4.37GATGLDD207 pKa = 4.2YY208 pKa = 11.06VTGTARR214 pKa = 11.84MSVDD218 pKa = 4.14FNDD221 pKa = 4.0FNQAPGVSLYY231 pKa = 11.04VVDD234 pKa = 3.95RR235 pKa = 11.84HH236 pKa = 6.85LYY238 pKa = 9.98DD239 pKa = 3.32INGNDD244 pKa = 3.12VTTEE248 pKa = 3.94YY249 pKa = 11.28LDD251 pKa = 4.58ALMTANTQGVRR262 pKa = 11.84PTNGLGQDD270 pKa = 3.99NLPDD274 pKa = 3.83INPVIRR280 pKa = 11.84IDD282 pKa = 3.57AADD285 pKa = 3.68TNGEE289 pKa = 4.13VTSEE293 pKa = 4.02VFTLLEE299 pKa = 4.57LDD301 pKa = 3.92NGSTTNLSSGNYY313 pKa = 7.12YY314 pKa = 10.79AIMAGDD320 pKa = 4.07GASEE324 pKa = 3.91IVGIVVMTGTDD335 pKa = 3.58PRR337 pKa = 11.84NSTSFEE343 pKa = 4.0EE344 pKa = 4.15TGGFIVTRR352 pKa = 4.18

Molecular weight: 37.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H5TV32|A0A1H5TV32_9RHOB Predicted dehydrogenase OS=Thalassococcus halodurans OX=373675 GN=SAMN04488045_0750 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNLVRR13 pKa = 11.84KK14 pKa = 9.18RR15 pKa = 11.84RR16 pKa = 11.84HH17 pKa = 4.42GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.37AGRR29 pKa = 11.84KK30 pKa = 8.54ILNARR35 pKa = 11.84RR36 pKa = 11.84ARR38 pKa = 11.84GRR40 pKa = 11.84KK41 pKa = 9.07SLSAA45 pKa = 3.93

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3829

0

3829

1203994

31

4044

314.4

34.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.515 ± 0.047

0.887 ± 0.012

6.443 ± 0.037

6.054 ± 0.036

3.845 ± 0.028

8.375 ± 0.04

1.99 ± 0.02

5.409 ± 0.029

3.698 ± 0.034

9.776 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.83 ± 0.021

2.897 ± 0.024

4.829 ± 0.028

3.343 ± 0.022

6.093 ± 0.042

5.44 ± 0.028

5.66 ± 0.036

7.294 ± 0.032

1.344 ± 0.016

2.277 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski