Saccharomyces kudriavzevii (strain ATCC MYA-4449 / AS 2.2408 / CBS 8840 / NBRC 1802 / NCYC 2889) (Yeast)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces; Saccharomyces kudriavzevii

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3745 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J6EQ84|J6EQ84_SACK1 Histone H2B OS=Saccharomyces kudriavzevii (strain ATCC MYA-4449 / AS 2.2408 / CBS 8840 / NBRC 1802 / NCYC 2889) OX=226230 GN=SKUD_158603 PE=3 SV=1
MM1 pKa = 7.89IITSTLVLTIIWSILPSSSDD21 pKa = 2.92EE22 pKa = 3.97HH23 pKa = 6.43TPQQFISTILDD34 pKa = 3.41IFPQRR39 pKa = 11.84RR40 pKa = 11.84WIITLEE46 pKa = 4.23SIMLMGMLCTYY57 pKa = 9.93TGLLMYY63 pKa = 10.63NEE65 pKa = 5.36DD66 pKa = 4.26SLTPPLDD73 pKa = 3.72SLCTVTDD80 pKa = 3.16AGGQLVIEE88 pKa = 5.54DD89 pKa = 4.11DD90 pKa = 3.61TDD92 pKa = 3.77VLVKK96 pKa = 10.2RR97 pKa = 11.84WAFKK101 pKa = 8.93EE102 pKa = 3.97TSGIYY107 pKa = 9.77DD108 pKa = 4.49LPLMDD113 pKa = 5.31ACQLLYY119 pKa = 10.83LYY121 pKa = 10.83DD122 pKa = 4.28NEE124 pKa = 4.42DD125 pKa = 3.18LSRR128 pKa = 5.19

Molecular weight: 14.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J4TT88|J4TT88_SACK1 Nucleolar complex-associated protein 3 OS=Saccharomyces kudriavzevii (strain ATCC MYA-4449 / AS 2.2408 / CBS 8840 / NBRC 1802 / NCYC 2889) OX=226230 GN=YLR002C PE=3 SV=1
MM1 pKa = 7.52LSSISSFSALSVLRR15 pKa = 11.84PQSSMLLNSPPMKK28 pKa = 10.19TMSLTALGFGFIGQRR43 pKa = 11.84RR44 pKa = 11.84WKK46 pKa = 10.67SRR48 pKa = 11.84GNTYY52 pKa = 10.31QPSTLKK58 pKa = 10.47RR59 pKa = 11.84KK60 pKa = 8.63RR61 pKa = 11.84TFGFLARR68 pKa = 11.84AKK70 pKa = 9.98SKK72 pKa = 10.64QGSKK76 pKa = 9.52ILKK79 pKa = 8.89RR80 pKa = 11.84RR81 pKa = 11.84KK82 pKa = 9.76LKK84 pKa = 10.58GRR86 pKa = 11.84WFLSHH91 pKa = 7.05

Molecular weight: 10.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3745

0

3745

1538632

45

2159

410.8

46.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.651 ± 0.04

1.31 ± 0.015

5.913 ± 0.029

6.561 ± 0.039

4.447 ± 0.027

5.196 ± 0.039

2.181 ± 0.016

6.417 ± 0.03

7.273 ± 0.039

9.525 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.084 ± 0.013

5.975 ± 0.039

4.342 ± 0.026

3.969 ± 0.032

4.566 ± 0.03

8.856 ± 0.055

5.703 ± 0.034

5.65 ± 0.026

1.043 ± 0.013

3.336 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski