Amino acid dipepetide frequency for Streptococcus phage LYGO9

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.206AlaAla: 3.206 ± 1.042
0.481AlaCys: 0.481 ± 0.234
3.607AlaAsp: 3.607 ± 0.65
4.489AlaGlu: 4.489 ± 0.727
2.405AlaPhe: 2.405 ± 0.427
4.168AlaGly: 4.168 ± 0.8
0.721AlaHis: 0.721 ± 0.262
5.37AlaIle: 5.37 ± 0.745
6.252AlaLys: 6.252 ± 1.012
6.092AlaLeu: 6.092 ± 0.773
1.844AlaMet: 1.844 ± 0.321
4.008AlaAsn: 4.008 ± 0.62
1.202AlaPro: 1.202 ± 0.336
2.405AlaGln: 2.405 ± 0.332
2.324AlaArg: 2.324 ± 0.478
4.248AlaSer: 4.248 ± 0.708
3.527AlaThr: 3.527 ± 0.639
3.687AlaVal: 3.687 ± 0.403
1.122AlaTrp: 1.122 ± 0.352
2.405AlaTyr: 2.405 ± 0.483
0.0AlaXaa: 0.0 ± 0.0
Cys
0.321CysAla: 0.321 ± 0.189
0.08CysCys: 0.08 ± 0.077
0.481CysAsp: 0.481 ± 0.205
0.802CysGlu: 0.802 ± 0.276
0.321CysPhe: 0.321 ± 0.175
0.641CysGly: 0.641 ± 0.262
0.08CysHis: 0.08 ± 0.089
0.401CysIle: 0.401 ± 0.183
0.401CysLys: 0.401 ± 0.206
0.481CysLeu: 0.481 ± 0.22
0.24CysMet: 0.24 ± 0.163
0.321CysAsn: 0.321 ± 0.144
0.08CysPro: 0.08 ± 0.077
0.08CysGln: 0.08 ± 0.076
0.16CysArg: 0.16 ± 0.11
0.401CysSer: 0.401 ± 0.168
0.561CysThr: 0.561 ± 0.187
0.321CysVal: 0.321 ± 0.155
0.16CysTrp: 0.16 ± 0.105
0.401CysTyr: 0.401 ± 0.179
0.0CysXaa: 0.0 ± 0.0
Asp
2.565AspAla: 2.565 ± 0.448
0.641AspCys: 0.641 ± 0.282
4.088AspAsp: 4.088 ± 0.737
5.691AspGlu: 5.691 ± 0.788
3.447AspPhe: 3.447 ± 0.487
4.729AspGly: 4.729 ± 0.639
0.641AspHis: 0.641 ± 0.23
4.008AspIle: 4.008 ± 0.574
6.012AspLys: 6.012 ± 0.741
5.931AspLeu: 5.931 ± 0.698
1.683AspMet: 1.683 ± 0.316
4.088AspAsn: 4.088 ± 0.485
1.122AspPro: 1.122 ± 0.341
1.523AspGln: 1.523 ± 0.357
2.565AspArg: 2.565 ± 0.436
4.088AspSer: 4.088 ± 0.644
3.286AspThr: 3.286 ± 0.525
3.527AspVal: 3.527 ± 0.56
0.802AspTrp: 0.802 ± 0.315
3.928AspTyr: 3.928 ± 0.684
0.0AspXaa: 0.0 ± 0.0
Glu
5.05GluAla: 5.05 ± 0.768
0.08GluCys: 0.08 ± 0.079
3.366GluAsp: 3.366 ± 0.55
6.653GluGlu: 6.653 ± 1.018
2.645GluPhe: 2.645 ± 0.438
2.324GluGly: 2.324 ± 0.421
0.721GluHis: 0.721 ± 0.238
6.813GluIle: 6.813 ± 0.831
6.733GluLys: 6.733 ± 0.947
7.855GluLeu: 7.855 ± 1.091
1.683GluMet: 1.683 ± 0.404
4.248GluAsn: 4.248 ± 0.736
1.523GluPro: 1.523 ± 0.41
4.088GluGln: 4.088 ± 0.641
2.164GluArg: 2.164 ± 0.446
4.649GluSer: 4.649 ± 0.648
4.328GluThr: 4.328 ± 0.508
5.29GluVal: 5.29 ± 0.614
1.202GluTrp: 1.202 ± 0.297
2.966GluTyr: 2.966 ± 0.55
0.0GluXaa: 0.0 ± 0.0
Phe
2.725PheAla: 2.725 ± 0.487
0.321PheCys: 0.321 ± 0.167
4.168PheAsp: 4.168 ± 0.711
2.886PheGlu: 2.886 ± 0.603
1.844PhePhe: 1.844 ± 0.509
3.286PheGly: 3.286 ± 0.468
0.0PheHis: 0.0 ± 0.0
2.565PheIle: 2.565 ± 0.405
4.649PheLys: 4.649 ± 0.705
2.324PheLeu: 2.324 ± 0.435
1.443PheMet: 1.443 ± 0.361
2.565PheAsn: 2.565 ± 0.435
1.042PhePro: 1.042 ± 0.243
0.721PheGln: 0.721 ± 0.26
1.603PheArg: 1.603 ± 0.306
3.286PheSer: 3.286 ± 0.508
2.244PheThr: 2.244 ± 0.509
2.565PheVal: 2.565 ± 0.416
0.401PheTrp: 0.401 ± 0.16
2.084PheTyr: 2.084 ± 0.387
0.0PheXaa: 0.0 ± 0.0
Gly
4.088GlyAla: 4.088 ± 0.757
0.16GlyCys: 0.16 ± 0.118
3.847GlyAsp: 3.847 ± 0.57
2.405GlyGlu: 2.405 ± 0.424
2.966GlyPhe: 2.966 ± 0.386
4.569GlyGly: 4.569 ± 0.85
0.802GlyHis: 0.802 ± 0.22
4.569GlyIle: 4.569 ± 0.487
5.611GlyLys: 5.611 ± 0.812
4.649GlyLeu: 4.649 ± 0.684
1.763GlyMet: 1.763 ± 0.417
4.008GlyAsn: 4.008 ± 0.413
1.042GlyPro: 1.042 ± 0.332
1.924GlyGln: 1.924 ± 0.378
2.164GlyArg: 2.164 ± 0.429
2.886GlySer: 2.886 ± 0.547
4.168GlyThr: 4.168 ± 0.822
4.649GlyVal: 4.649 ± 0.685
1.122GlyTrp: 1.122 ± 0.243
3.366GlyTyr: 3.366 ± 0.577
0.0GlyXaa: 0.0 ± 0.0
His
0.481HisAla: 0.481 ± 0.243
0.16HisCys: 0.16 ± 0.111
0.882HisAsp: 0.882 ± 0.315
1.042HisGlu: 1.042 ± 0.301
0.401HisPhe: 0.401 ± 0.158
0.641HisGly: 0.641 ± 0.226
0.401HisHis: 0.401 ± 0.205
0.641HisIle: 0.641 ± 0.274
0.721HisLys: 0.721 ± 0.174
1.122HisLeu: 1.122 ± 0.26
0.08HisMet: 0.08 ± 0.095
0.802HisAsn: 0.802 ± 0.236
0.24HisPro: 0.24 ± 0.115
0.721HisGln: 0.721 ± 0.19
0.641HisArg: 0.641 ± 0.233
0.721HisSer: 0.721 ± 0.266
0.481HisThr: 0.481 ± 0.232
0.721HisVal: 0.721 ± 0.22
0.24HisTrp: 0.24 ± 0.143
0.641HisTyr: 0.641 ± 0.221
0.0HisXaa: 0.0 ± 0.0
Ile
5.771IleAla: 5.771 ± 0.521
0.641IleCys: 0.641 ± 0.254
5.931IleAsp: 5.931 ± 0.62
5.851IleGlu: 5.851 ± 0.971
3.366IlePhe: 3.366 ± 0.686
3.286IleGly: 3.286 ± 0.684
0.481IleHis: 0.481 ± 0.193
3.767IleIle: 3.767 ± 0.716
6.893IleLys: 6.893 ± 0.691
4.729IleLeu: 4.729 ± 0.806
1.282IleMet: 1.282 ± 0.27
4.569IleAsn: 4.569 ± 0.613
2.405IlePro: 2.405 ± 0.422
1.603IleGln: 1.603 ± 0.368
2.966IleArg: 2.966 ± 0.514
3.928IleSer: 3.928 ± 0.481
4.248IleThr: 4.248 ± 0.534
3.767IleVal: 3.767 ± 0.545
0.882IleTrp: 0.882 ± 0.258
2.886IleTyr: 2.886 ± 0.506
0.0IleXaa: 0.0 ± 0.0
Lys
6.813LysAla: 6.813 ± 0.892
0.401LysCys: 0.401 ± 0.168
5.771LysAsp: 5.771 ± 0.655
7.935LysGlu: 7.935 ± 1.102
3.286LysPhe: 3.286 ± 0.696
5.29LysGly: 5.29 ± 0.79
1.202LysHis: 1.202 ± 0.316
5.931LysIle: 5.931 ± 0.834
8.015LysLys: 8.015 ± 0.747
7.294LysLeu: 7.294 ± 0.716
1.844LysMet: 1.844 ± 0.399
5.37LysAsn: 5.37 ± 0.564
2.725LysPro: 2.725 ± 0.595
3.847LysGln: 3.847 ± 0.686
3.527LysArg: 3.527 ± 0.648
5.21LysSer: 5.21 ± 0.578
6.973LysThr: 6.973 ± 0.671
5.13LysVal: 5.13 ± 0.606
0.962LysTrp: 0.962 ± 0.242
3.286LysTyr: 3.286 ± 0.467
0.0LysXaa: 0.0 ± 0.0
Leu
5.611LeuAla: 5.611 ± 0.669
0.481LeuCys: 0.481 ± 0.198
5.691LeuAsp: 5.691 ± 0.615
6.573LeuGlu: 6.573 ± 0.884
2.886LeuPhe: 2.886 ± 0.525
5.13LeuGly: 5.13 ± 0.932
0.802LeuHis: 0.802 ± 0.228
5.13LeuIle: 5.13 ± 0.561
7.615LeuLys: 7.615 ± 0.938
5.691LeuLeu: 5.691 ± 0.698
2.244LeuMet: 2.244 ± 0.461
5.771LeuAsn: 5.771 ± 0.768
2.565LeuPro: 2.565 ± 0.489
3.687LeuGln: 3.687 ± 0.545
3.366LeuArg: 3.366 ± 0.647
5.851LeuSer: 5.851 ± 0.661
5.45LeuThr: 5.45 ± 0.719
4.168LeuVal: 4.168 ± 0.494
0.882LeuTrp: 0.882 ± 0.241
3.126LeuTyr: 3.126 ± 0.459
0.0LeuXaa: 0.0 ± 0.0
Met
1.603MetAla: 1.603 ± 0.278
0.16MetCys: 0.16 ± 0.129
1.042MetAsp: 1.042 ± 0.358
1.282MetGlu: 1.282 ± 0.267
1.363MetPhe: 1.363 ± 0.373
0.721MetGly: 0.721 ± 0.27
0.16MetHis: 0.16 ± 0.086
2.324MetIle: 2.324 ± 0.506
2.324MetLys: 2.324 ± 0.532
1.763MetLeu: 1.763 ± 0.299
0.721MetMet: 0.721 ± 0.197
1.523MetAsn: 1.523 ± 0.347
0.401MetPro: 0.401 ± 0.181
1.122MetGln: 1.122 ± 0.244
1.202MetArg: 1.202 ± 0.354
1.683MetSer: 1.683 ± 0.414
2.004MetThr: 2.004 ± 0.448
1.924MetVal: 1.924 ± 0.334
0.321MetTrp: 0.321 ± 0.134
0.721MetTyr: 0.721 ± 0.246
0.0MetXaa: 0.0 ± 0.0
Asn
3.447AsnAla: 3.447 ± 0.62
0.24AsnCys: 0.24 ± 0.129
3.286AsnAsp: 3.286 ± 0.499
5.13AsnGlu: 5.13 ± 0.642
2.645AsnPhe: 2.645 ± 0.466
4.97AsnGly: 4.97 ± 0.788
0.962AsnHis: 0.962 ± 0.409
4.649AsnIle: 4.649 ± 0.61
5.851AsnLys: 5.851 ± 0.735
4.97AsnLeu: 4.97 ± 0.599
1.202AsnMet: 1.202 ± 0.334
3.046AsnAsn: 3.046 ± 0.453
2.725AsnPro: 2.725 ± 0.311
1.924AsnGln: 1.924 ± 0.433
2.725AsnArg: 2.725 ± 0.574
4.008AsnSer: 4.008 ± 0.879
2.645AsnThr: 2.645 ± 0.462
3.767AsnVal: 3.767 ± 0.63
0.802AsnTrp: 0.802 ± 0.305
2.485AsnTyr: 2.485 ± 0.399
0.0AsnXaa: 0.0 ± 0.0
Pro
0.882ProAla: 0.882 ± 0.3
0.08ProCys: 0.08 ± 0.079
2.164ProAsp: 2.164 ± 0.425
2.084ProGlu: 2.084 ± 0.502
1.282ProPhe: 1.282 ± 0.265
0.882ProGly: 0.882 ± 0.416
0.401ProHis: 0.401 ± 0.208
1.683ProIle: 1.683 ± 0.317
2.405ProLys: 2.405 ± 0.47
2.164ProLeu: 2.164 ± 0.444
0.24ProMet: 0.24 ± 0.14
1.683ProAsn: 1.683 ± 0.385
0.641ProPro: 0.641 ± 0.234
1.202ProGln: 1.202 ± 0.35
0.721ProArg: 0.721 ± 0.302
2.405ProSer: 2.405 ± 0.468
1.363ProThr: 1.363 ± 0.281
1.443ProVal: 1.443 ± 0.367
0.0ProTrp: 0.0 ± 0.0
1.603ProTyr: 1.603 ± 0.428
0.0ProXaa: 0.0 ± 0.0
Gln
3.206GlnAla: 3.206 ± 0.554
0.401GlnCys: 0.401 ± 0.215
1.443GlnAsp: 1.443 ± 0.247
2.405GlnGlu: 2.405 ± 0.408
1.844GlnPhe: 1.844 ± 0.373
1.924GlnGly: 1.924 ± 0.343
0.321GlnHis: 0.321 ± 0.15
3.366GlnIle: 3.366 ± 0.52
3.447GlnLys: 3.447 ± 0.517
4.088GlnLeu: 4.088 ± 0.456
1.122GlnMet: 1.122 ± 0.291
2.164GlnAsn: 2.164 ± 0.432
0.721GlnPro: 0.721 ± 0.213
1.924GlnGln: 1.924 ± 0.568
1.443GlnArg: 1.443 ± 0.37
3.206GlnSer: 3.206 ± 0.471
1.924GlnThr: 1.924 ± 0.35
1.683GlnVal: 1.683 ± 0.38
0.721GlnTrp: 0.721 ± 0.186
1.042GlnTyr: 1.042 ± 0.273
0.0GlnXaa: 0.0 ± 0.0
Arg
1.763ArgAla: 1.763 ± 0.37
0.321ArgCys: 0.321 ± 0.181
2.324ArgAsp: 2.324 ± 0.401
3.366ArgGlu: 3.366 ± 0.481
1.683ArgPhe: 1.683 ± 0.276
1.763ArgGly: 1.763 ± 0.389
0.962ArgHis: 0.962 ± 0.285
3.847ArgIle: 3.847 ± 0.579
3.286ArgLys: 3.286 ± 0.735
3.126ArgLeu: 3.126 ± 0.481
1.202ArgMet: 1.202 ± 0.281
1.924ArgAsn: 1.924 ± 0.373
1.122ArgPro: 1.122 ± 0.363
1.763ArgGln: 1.763 ± 0.346
2.164ArgArg: 2.164 ± 0.465
1.844ArgSer: 1.844 ± 0.365
2.645ArgThr: 2.645 ± 0.393
1.924ArgVal: 1.924 ± 0.351
0.561ArgTrp: 0.561 ± 0.226
1.924ArgTyr: 1.924 ± 0.431
0.0ArgXaa: 0.0 ± 0.0
Ser
3.767SerAla: 3.767 ± 0.682
0.08SerCys: 0.08 ± 0.085
5.45SerAsp: 5.45 ± 0.67
4.008SerGlu: 4.008 ± 0.478
3.447SerPhe: 3.447 ± 0.577
4.809SerGly: 4.809 ± 0.82
1.363SerHis: 1.363 ± 0.301
3.847SerIle: 3.847 ± 0.538
5.13SerLys: 5.13 ± 0.688
5.37SerLeu: 5.37 ± 0.585
1.523SerMet: 1.523 ± 0.367
4.168SerAsn: 4.168 ± 0.739
0.721SerPro: 0.721 ± 0.273
2.805SerGln: 2.805 ± 0.437
2.324SerArg: 2.324 ± 0.412
5.21SerSer: 5.21 ± 0.556
3.767SerThr: 3.767 ± 0.669
3.607SerVal: 3.607 ± 0.59
0.641SerTrp: 0.641 ± 0.203
3.447SerTyr: 3.447 ± 0.566
0.0SerXaa: 0.0 ± 0.0
Thr
4.168ThrAla: 4.168 ± 0.802
0.321ThrCys: 0.321 ± 0.149
3.206ThrAsp: 3.206 ± 0.384
3.447ThrGlu: 3.447 ± 0.472
2.725ThrPhe: 2.725 ± 0.537
5.13ThrGly: 5.13 ± 0.745
0.401ThrHis: 0.401 ± 0.203
4.168ThrIle: 4.168 ± 0.736
5.851ThrLys: 5.851 ± 0.694
5.45ThrLeu: 5.45 ± 0.444
1.523ThrMet: 1.523 ± 0.437
4.088ThrAsn: 4.088 ± 0.515
2.084ThrPro: 2.084 ± 0.384
2.324ThrGln: 2.324 ± 0.47
2.805ThrArg: 2.805 ± 0.481
3.126ThrSer: 3.126 ± 0.617
4.328ThrThr: 4.328 ± 0.548
5.13ThrVal: 5.13 ± 0.923
0.561ThrTrp: 0.561 ± 0.194
2.084ThrTyr: 2.084 ± 0.507
0.0ThrXaa: 0.0 ± 0.0
Val
4.809ValAla: 4.809 ± 0.557
0.321ValCys: 0.321 ± 0.136
4.168ValAsp: 4.168 ± 0.481
4.489ValGlu: 4.489 ± 0.592
1.523ValPhe: 1.523 ± 0.366
3.527ValGly: 3.527 ± 0.527
0.641ValHis: 0.641 ± 0.204
3.286ValIle: 3.286 ± 0.494
5.13ValLys: 5.13 ± 0.643
4.889ValLeu: 4.889 ± 0.78
1.443ValMet: 1.443 ± 0.347
4.008ValAsn: 4.008 ± 0.432
1.042ValPro: 1.042 ± 0.284
1.844ValGln: 1.844 ± 0.505
2.565ValArg: 2.565 ± 0.527
5.29ValSer: 5.29 ± 0.828
5.13ValThr: 5.13 ± 0.617
4.408ValVal: 4.408 ± 0.788
0.401ValTrp: 0.401 ± 0.163
1.763ValTyr: 1.763 ± 0.273
0.0ValXaa: 0.0 ± 0.0
Trp
0.721TrpAla: 0.721 ± 0.222
0.321TrpCys: 0.321 ± 0.165
0.641TrpAsp: 0.641 ± 0.225
0.882TrpGlu: 0.882 ± 0.33
0.481TrpPhe: 0.481 ± 0.178
1.042TrpGly: 1.042 ± 0.39
0.16TrpHis: 0.16 ± 0.104
0.641TrpIle: 0.641 ± 0.262
0.882TrpLys: 0.882 ± 0.25
1.122TrpLeu: 1.122 ± 0.312
0.24TrpMet: 0.24 ± 0.127
0.481TrpAsn: 0.481 ± 0.253
0.08TrpPro: 0.08 ± 0.072
0.641TrpGln: 0.641 ± 0.229
0.641TrpArg: 0.641 ± 0.243
0.962TrpSer: 0.962 ± 0.25
1.042TrpThr: 1.042 ± 0.379
0.802TrpVal: 0.802 ± 0.232
0.16TrpTrp: 0.16 ± 0.091
0.401TrpTyr: 0.401 ± 0.168
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.565TyrAla: 2.565 ± 0.549
1.042TyrCys: 1.042 ± 0.257
2.805TyrAsp: 2.805 ± 0.469
2.966TyrGlu: 2.966 ± 0.694
2.244TyrPhe: 2.244 ± 0.426
1.924TyrGly: 1.924 ± 0.355
0.561TyrHis: 0.561 ± 0.159
2.244TyrIle: 2.244 ± 0.526
3.607TyrLys: 3.607 ± 0.524
3.607TyrLeu: 3.607 ± 0.542
0.962TyrMet: 0.962 ± 0.272
2.645TyrAsn: 2.645 ± 0.583
1.844TyrPro: 1.844 ± 0.381
2.244TyrGln: 2.244 ± 0.38
1.443TyrArg: 1.443 ± 0.431
2.485TyrSer: 2.485 ± 0.413
2.805TyrThr: 2.805 ± 0.462
2.164TyrVal: 2.164 ± 0.4
0.401TyrTrp: 0.401 ± 0.192
1.844TyrTyr: 1.844 ± 0.432
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 66 proteins (12477 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski