Marssonina brunnea f. sp. multigermtubi (strain MB_m1) (Marssonina leaf spot fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Helotiales; Dermateaceae; Marssonina; Marssonina brunnea; Marssonina brunnea f. sp. 'multigermtubi'

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10037 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K1X7G9|K1X7G9_MARBU BZIP-type transcription factor OS=Marssonina brunnea f. sp. multigermtubi (strain MB_m1) OX=1072389 GN=MBM_00160 PE=4 SV=1
MM1 pKa = 8.31DD2 pKa = 4.76MLTVQYY8 pKa = 11.21KK9 pKa = 10.53LLANSARR16 pKa = 11.84LAFTPSKK23 pKa = 10.2YY24 pKa = 9.97RR25 pKa = 11.84YY26 pKa = 8.77SALHH30 pKa = 4.65STPRR34 pKa = 11.84SFPNQPRR41 pKa = 11.84YY42 pKa = 8.18TYY44 pKa = 10.8QPDD47 pKa = 5.27DD48 pKa = 4.96DD49 pKa = 6.04DD50 pKa = 5.36DD51 pKa = 4.16WLPSPRR57 pKa = 11.84PSVPRR62 pKa = 11.84FSSPRR67 pKa = 11.84FQQAQQAQQAQQAQQPQYY85 pKa = 11.01DD86 pKa = 3.72YY87 pKa = 11.08DD88 pKa = 4.34YY89 pKa = 9.88EE90 pKa = 4.22TEE92 pKa = 4.09EE93 pKa = 3.98QIGYY97 pKa = 10.27AEE99 pKa = 4.2QIEE102 pKa = 4.28EE103 pKa = 4.06LRR105 pKa = 11.84EE106 pKa = 3.89EE107 pKa = 4.38FEE109 pKa = 6.55DD110 pKa = 4.21NSEE113 pKa = 4.22DD114 pKa = 3.84QNEE117 pKa = 4.09DD118 pKa = 3.43EE119 pKa = 4.91EE120 pKa = 5.93DD121 pKa = 3.81YY122 pKa = 11.74EE123 pKa = 4.22EE124 pKa = 6.06DD125 pKa = 4.02YY126 pKa = 11.51EE127 pKa = 4.83SDD129 pKa = 3.66YY130 pKa = 11.87DD131 pKa = 4.66DD132 pKa = 6.27GYY134 pKa = 11.56DD135 pKa = 3.95DD136 pKa = 5.98GEE138 pKa = 4.18WW139 pKa = 3.16

Molecular weight: 16.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K1W5Y6|K1W5Y6_MARBU Ammecr1 family protein OS=Marssonina brunnea f. sp. multigermtubi (strain MB_m1) OX=1072389 GN=MBM_09394 PE=4 SV=1
MM1 pKa = 6.82GQLRR5 pKa = 11.84VVALRR10 pKa = 11.84TLRR13 pKa = 11.84MAALRR18 pKa = 11.84GLRR21 pKa = 11.84VAALTVILNVLKK33 pKa = 10.72AAIRR37 pKa = 11.84KK38 pKa = 8.37LRR40 pKa = 11.84KK41 pKa = 8.98AAIRR45 pKa = 11.84NLRR48 pKa = 11.84KK49 pKa = 9.47AAILNVLKK57 pKa = 10.05ATTRR61 pKa = 11.84NPRR64 pKa = 11.84KK65 pKa = 9.57AAIRR69 pKa = 11.84NLRR72 pKa = 11.84KK73 pKa = 9.5AATFNVLKK81 pKa = 10.67AAIRR85 pKa = 11.84NLRR88 pKa = 11.84KK89 pKa = 9.57AATLNVLKK97 pKa = 10.71AVIRR101 pKa = 11.84NPRR104 pKa = 11.84KK105 pKa = 9.05AAIRR109 pKa = 11.84SVRR112 pKa = 11.84KK113 pKa = 9.12AAIRR117 pKa = 11.84NLLVNYY123 pKa = 8.54SLRR126 pKa = 11.84AII128 pKa = 4.2

Molecular weight: 14.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10037

0

10037

4975927

50

6298

495.8

54.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.795 ± 0.024

1.162 ± 0.009

5.511 ± 0.016

6.382 ± 0.027

3.616 ± 0.014

7.036 ± 0.025

2.246 ± 0.01

4.841 ± 0.016

5.227 ± 0.025

8.656 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.191 ± 0.009

3.648 ± 0.014

6.255 ± 0.028

3.892 ± 0.017

6.219 ± 0.021

8.488 ± 0.033

5.928 ± 0.018

5.943 ± 0.017

1.322 ± 0.009

2.641 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski