Sphingobium japonicum (strain DSM 16413 / CCM 7287 / MTCC 6362 / UT26 / NBRC 101211 / UT26S)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingobium; Sphingobium indicum

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4347 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D4Z202|D4Z202_SPHJU TrbN/BfpH-like protein OS=Sphingobium japonicum (strain DSM 16413 / CCM 7287 / MTCC 6362 / UT26 / NBRC 101211 / UT26S) OX=452662 GN=SJA_C1-18000 PE=4 SV=1
MM1 pKa = 7.7TYY3 pKa = 10.26GHH5 pKa = 6.55SVTIDD10 pKa = 3.11GSLDD14 pKa = 2.97DD15 pKa = 4.41WFATDD20 pKa = 5.81RR21 pKa = 11.84IDD23 pKa = 5.21DD24 pKa = 3.89NSAPGYY30 pKa = 9.28QVYY33 pKa = 7.91ATLDD37 pKa = 3.03NDD39 pKa = 3.86AFVFALTAPVAIGADD54 pKa = 3.38TTVWLNTDD62 pKa = 3.62GKK64 pKa = 9.32TSTGYY69 pKa = 10.64QIFGFAGGAEE79 pKa = 4.26YY80 pKa = 10.84NVNFAADD87 pKa = 3.84GTVNLYY93 pKa = 10.61SGAAGEE99 pKa = 4.19TLVLGGLQAAWSADD113 pKa = 3.2RR114 pKa = 11.84TTVEE118 pKa = 3.96FRR120 pKa = 11.84LPTSAVGNPAAMYY133 pKa = 10.12SYY135 pKa = 11.65YY136 pKa = 10.69DD137 pKa = 3.56INNLSNSFMPSNYY150 pKa = 8.91QSAPFTVFNQTTATQAPDD168 pKa = 3.24MRR170 pKa = 11.84IGIVFSQTTADD181 pKa = 3.82AYY183 pKa = 10.59FSPTAYY189 pKa = 9.99SQLFMSVQAQAIQAGIPFDD208 pKa = 3.74ILTEE212 pKa = 4.37ADD214 pKa = 3.6LTDD217 pKa = 4.26LNKK220 pKa = 10.43LSHH223 pKa = 6.51YY224 pKa = 9.61DD225 pKa = 3.36ALVFPSFRR233 pKa = 11.84NVPVDD238 pKa = 3.22KK239 pKa = 11.29VEE241 pKa = 5.14AISHH245 pKa = 4.93TLEE248 pKa = 4.06MASKK252 pKa = 10.13QLGIGLITAGEE263 pKa = 4.14FMTNQPHH270 pKa = 5.64VPGGAIGGDD279 pKa = 4.09PLPGDD284 pKa = 4.91PYY286 pKa = 11.72AQMKK290 pKa = 10.45LLFDD294 pKa = 3.87ATRR297 pKa = 11.84VTGGTGDD304 pKa = 3.84VVVTATDD311 pKa = 3.56PTGLVLDD318 pKa = 5.23HH319 pKa = 6.54YY320 pKa = 11.91ANGQQVNSYY329 pKa = 11.03SGVGWNAFASVSGTGHH345 pKa = 6.44QIATEE350 pKa = 4.42TINGGSAYY358 pKa = 10.08AAALATQTGAARR370 pKa = 11.84NVLFSSEE377 pKa = 3.75GVMADD382 pKa = 4.33ANMLQQAISYY392 pKa = 8.11AANGTGLSVGLHH404 pKa = 6.58LSRR407 pKa = 11.84EE408 pKa = 4.38SGVVAARR415 pKa = 11.84VDD417 pKa = 3.45MDD419 pKa = 3.35QSQEE423 pKa = 4.17LEE425 pKa = 4.4DD426 pKa = 3.84VDD428 pKa = 5.0PPGSTPGIYY437 pKa = 10.21DD438 pKa = 3.48KK439 pKa = 11.03LLPILTQWKK448 pKa = 9.71ADD450 pKa = 3.66YY451 pKa = 10.88NFVGSFYY458 pKa = 11.03LNVGNGTNGTGTDD471 pKa = 3.38WAVSLPYY478 pKa = 10.58YY479 pKa = 10.32KK480 pKa = 10.85ALLDD484 pKa = 4.35LGNEE488 pKa = 4.38LGTHH492 pKa = 6.09SLTHH496 pKa = 7.0PDD498 pKa = 3.09NTNLLNAQQIQTEE511 pKa = 4.41FQDD514 pKa = 4.37SIAILNQQISAYY526 pKa = 10.08LNQPFQITGAAVPGAPEE543 pKa = 4.11SLPVSQEE550 pKa = 3.05IMKK553 pKa = 10.38YY554 pKa = 10.05VSDD557 pKa = 4.24YY558 pKa = 10.76LTGGYY563 pKa = 9.32AAQGAGYY570 pKa = 8.77PNAFGYY576 pKa = 7.41LTPADD581 pKa = 3.35QSKK584 pKa = 10.57IYY586 pKa = 9.4FAPNTSFDD594 pKa = 3.39YY595 pKa = 11.03TFIEE599 pKa = 4.92FQQKK603 pKa = 7.0TVAEE607 pKa = 4.48ATALWIGEE615 pKa = 3.92FDD617 pKa = 4.14KK618 pKa = 11.48LVANAGMPVIVWPWHH633 pKa = 6.94DD634 pKa = 3.57YY635 pKa = 10.36GAADD639 pKa = 4.58FLSGSPTVDD648 pKa = 2.81SPYY651 pKa = 10.57DD652 pKa = 3.35ISLFTSWIQHH662 pKa = 4.4AVAANMEE669 pKa = 4.58FVTLDD674 pKa = 3.39DD675 pKa = 3.79LAARR679 pKa = 11.84MTAVKK684 pKa = 10.21NATITTTVAGNSITASVGGANVGNLSLDD712 pKa = 3.64VEE714 pKa = 4.49RR715 pKa = 11.84QGALVIQNVTGWYY728 pKa = 9.97AYY730 pKa = 10.57DD731 pKa = 3.64SDD733 pKa = 5.55SVFLPQSGGTFKK745 pKa = 9.84ITLGAAADD753 pKa = 4.2DD754 pKa = 4.07VTHH757 pKa = 6.31ITDD760 pKa = 3.66LPMRR764 pKa = 11.84AVLLSVAGDD773 pKa = 3.42GHH775 pKa = 6.51NLAFSIQGEE784 pKa = 4.55GKK786 pKa = 10.33VVIDD790 pKa = 3.9IANPGTDD797 pKa = 3.75GIVVTGAAGYY807 pKa = 8.64SQVGEE812 pKa = 4.24IVTVDD817 pKa = 3.19LGGYY821 pKa = 8.58GLHH824 pKa = 6.52NVMLEE829 pKa = 4.09YY830 pKa = 10.67KK831 pKa = 9.21NPPVIVSNGGGPTATLSMGEE851 pKa = 4.19NGTAVTTVVATDD863 pKa = 3.59ADD865 pKa = 3.83VGTVIGYY872 pKa = 9.33SISGGADD879 pKa = 2.87AAKK882 pKa = 10.28FRR884 pKa = 11.84IDD886 pKa = 3.38GATGALTFLSAPNFEE901 pKa = 4.5AAGDD905 pKa = 3.74VGTDD909 pKa = 3.01NVYY912 pKa = 11.12DD913 pKa = 4.11VIVRR917 pKa = 11.84ASDD920 pKa = 3.12GTLYY924 pKa = 10.68DD925 pKa = 3.86DD926 pKa = 3.62QAIAVTITNVNEE938 pKa = 4.03APVITSNGGGASAAVSVAEE957 pKa = 4.16NLTGVTTVTAIDD969 pKa = 4.0PDD971 pKa = 4.25AGTMLAYY978 pKa = 10.45SIVGGPDD985 pKa = 2.74RR986 pKa = 11.84ALFSIDD992 pKa = 3.59PSTGALAFLSPPNYY1006 pKa = 10.02DD1007 pKa = 3.99LPGDD1011 pKa = 3.83ANGNNVYY1018 pKa = 10.62SLTVRR1023 pKa = 11.84VSDD1026 pKa = 3.91GALFDD1031 pKa = 4.18DD1032 pKa = 3.92QVINVTVTNVNEE1044 pKa = 3.96QAPVITSGGGGDD1056 pKa = 3.39TAAISVSSGTRR1067 pKa = 11.84TVMKK1071 pKa = 9.27VTAVDD1076 pKa = 3.51PDD1078 pKa = 4.0ANTTLAYY1085 pKa = 10.36SIAGGVDD1092 pKa = 2.76AGKK1095 pKa = 8.57FTINASTGQLNFVSTPNYY1113 pKa = 10.27LLPTDD1118 pKa = 4.13AGRR1121 pKa = 11.84DD1122 pKa = 3.39NVYY1125 pKa = 10.97DD1126 pKa = 4.04VIVRR1130 pKa = 11.84ASDD1133 pKa = 3.47GTLHH1137 pKa = 7.35DD1138 pKa = 4.0EE1139 pKa = 3.91QAIAVTVTILNAPPIIISNGGGASATIRR1167 pKa = 11.84MAEE1170 pKa = 4.19NGTAVTKK1177 pKa = 9.35VTATDD1182 pKa = 3.41VNLGQSITYY1191 pKa = 10.06SLGSAVDD1198 pKa = 3.7GALFSIDD1205 pKa = 3.73PQSGVLTFKK1214 pKa = 10.53SAPDD1218 pKa = 3.74FEE1220 pKa = 6.3APADD1224 pKa = 3.87SNLDD1228 pKa = 2.83NRR1230 pKa = 11.84YY1231 pKa = 8.85QVQVLATDD1239 pKa = 3.44SLGAQDD1245 pKa = 4.31FQNLTVVVNNVSGLSLTAASGGSTLIGQGEE1275 pKa = 4.67EE1276 pKa = 3.94DD1277 pKa = 3.82TLRR1280 pKa = 11.84GAGGNDD1286 pKa = 3.26TLIGNGGSDD1295 pKa = 3.73TLNGAGGNDD1304 pKa = 4.11NIDD1307 pKa = 3.63GGAGADD1313 pKa = 4.32RR1314 pKa = 11.84ITGGAGADD1322 pKa = 3.5ILTGGADD1329 pKa = 3.7ADD1331 pKa = 4.0LFIYY1335 pKa = 10.42NATGNSTLAVMDD1347 pKa = 5.38RR1348 pKa = 11.84IMDD1351 pKa = 4.11FQQGIDD1357 pKa = 3.95RR1358 pKa = 11.84IDD1360 pKa = 3.57LSGIDD1365 pKa = 4.14ALSALSGNQAFSFIGNAAFSGAGQLHH1391 pKa = 6.42VYY1393 pKa = 10.97SNGTDD1398 pKa = 3.18SFVEE1402 pKa = 4.04ANVDD1406 pKa = 3.7NNPATVDD1413 pKa = 3.7FAIRR1417 pKa = 11.84LIGVHH1422 pKa = 6.35NLTASDD1428 pKa = 3.96FLLL1431 pKa = 4.74

Molecular weight: 148.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D4YZ56|D4YZ56_SPHJU Uncharacterized protein OS=Sphingobium japonicum (strain DSM 16413 / CCM 7287 / MTCC 6362 / UT26 / NBRC 101211 / UT26S) OX=452662 GN=SJA_C1-08040 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATPGGRR28 pKa = 11.84NVLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4347

0

4347

1313854

26

4159

302.2

32.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.205 ± 0.061

0.811 ± 0.013

5.946 ± 0.037

5.353 ± 0.038

3.527 ± 0.024

9.0 ± 0.048

2.069 ± 0.021

5.045 ± 0.023

2.964 ± 0.028

9.98 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.557 ± 0.02

2.547 ± 0.03

5.347 ± 0.036

3.209 ± 0.023

7.664 ± 0.046

5.345 ± 0.029

4.9 ± 0.039

6.909 ± 0.029

1.421 ± 0.017

2.202 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski