Amino acid dipepetide frequency for Actinomadura sp. CNU-125

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.011AlaAla: 22.011 ± 0.171
1.168AlaCys: 1.168 ± 0.025
8.935AlaAsp: 8.935 ± 0.071
8.897AlaGlu: 8.897 ± 0.079
3.786AlaPhe: 3.786 ± 0.047
13.265AlaGly: 13.265 ± 0.096
2.689AlaHis: 2.689 ± 0.035
4.031AlaIle: 4.031 ± 0.052
2.545AlaLys: 2.545 ± 0.042
13.492AlaLeu: 13.492 ± 0.105
2.707AlaMet: 2.707 ± 0.033
1.928AlaAsn: 1.928 ± 0.031
7.046AlaPro: 7.046 ± 0.066
2.975AlaGln: 2.975 ± 0.038
11.512AlaArg: 11.512 ± 0.094
5.898AlaSer: 5.898 ± 0.057
6.421AlaThr: 6.421 ± 0.058
11.75AlaVal: 11.75 ± 0.091
1.92AlaTrp: 1.92 ± 0.028
2.458AlaTyr: 2.458 ± 0.036
0.0AlaXaa: 0.0 ± 0.0
Cys
1.096CysAla: 1.096 ± 0.024
0.143CysCys: 0.143 ± 0.008
0.516CysAsp: 0.516 ± 0.017
0.446CysGlu: 0.446 ± 0.015
0.215CysPhe: 0.215 ± 0.011
1.091CysGly: 1.091 ± 0.028
0.189CysHis: 0.189 ± 0.01
0.179CysIle: 0.179 ± 0.009
0.117CysLys: 0.117 ± 0.008
0.678CysLeu: 0.678 ± 0.02
0.154CysMet: 0.154 ± 0.009
0.112CysAsn: 0.112 ± 0.007
0.551CysPro: 0.551 ± 0.018
0.144CysGln: 0.144 ± 0.008
0.718CysArg: 0.718 ± 0.021
0.502CysSer: 0.502 ± 0.015
0.551CysThr: 0.551 ± 0.018
0.672CysVal: 0.672 ± 0.017
0.144CysTrp: 0.144 ± 0.009
0.153CysTyr: 0.153 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
8.653AspAla: 8.653 ± 0.069
0.399AspCys: 0.399 ± 0.013
4.487AspAsp: 4.487 ± 0.053
4.438AspGlu: 4.438 ± 0.048
1.557AspPhe: 1.557 ± 0.025
7.358AspGly: 7.358 ± 0.067
1.392AspHis: 1.392 ± 0.03
1.766AspIle: 1.766 ± 0.03
0.905AspLys: 0.905 ± 0.021
6.531AspLeu: 6.531 ± 0.068
0.977AspMet: 0.977 ± 0.021
0.79AspAsn: 0.79 ± 0.019
4.839AspPro: 4.839 ± 0.051
1.283AspGln: 1.283 ± 0.025
5.742AspArg: 5.742 ± 0.06
2.024AspSer: 2.024 ± 0.033
2.627AspThr: 2.627 ± 0.043
5.642AspVal: 5.642 ± 0.054
0.946AspTrp: 0.946 ± 0.021
1.103AspTyr: 1.103 ± 0.024
0.0AspXaa: 0.0 ± 0.0
Glu
6.702GluAla: 6.702 ± 0.063
0.405GluCys: 0.405 ± 0.014
2.826GluAsp: 2.826 ± 0.037
3.193GluGlu: 3.193 ± 0.043
1.692GluPhe: 1.692 ± 0.027
4.035GluGly: 4.035 ± 0.046
1.7GluHis: 1.7 ± 0.028
2.536GluIle: 2.536 ± 0.035
1.32GluLys: 1.32 ± 0.028
6.587GluLeu: 6.587 ± 0.068
1.034GluMet: 1.034 ± 0.025
1.058GluAsn: 1.058 ± 0.025
3.848GluPro: 3.848 ± 0.045
1.975GluGln: 1.975 ± 0.032
6.172GluArg: 6.172 ± 0.061
2.535GluSer: 2.535 ± 0.041
3.034GluThr: 3.034 ± 0.04
4.472GluVal: 4.472 ± 0.051
0.837GluTrp: 0.837 ± 0.022
1.187GluTyr: 1.187 ± 0.025
0.0GluXaa: 0.0 ± 0.0
Phe
4.03PheAla: 4.03 ± 0.043
0.307PheCys: 0.307 ± 0.011
2.109PheAsp: 2.109 ± 0.033
1.621PheGlu: 1.621 ± 0.03
0.87PhePhe: 0.87 ± 0.022
3.312PheGly: 3.312 ± 0.038
0.58PheHis: 0.58 ± 0.019
0.714PheIle: 0.714 ± 0.021
0.482PheLys: 0.482 ± 0.015
2.572PheLeu: 2.572 ± 0.042
0.45PheMet: 0.45 ± 0.017
0.514PheAsn: 0.514 ± 0.014
1.403PhePro: 1.403 ± 0.027
0.675PheGln: 0.675 ± 0.018
1.961PheArg: 1.961 ± 0.029
1.278PheSer: 1.278 ± 0.023
1.925PheThr: 1.925 ± 0.031
2.505PheVal: 2.505 ± 0.036
0.442PheTrp: 0.442 ± 0.014
0.594PheTyr: 0.594 ± 0.017
0.0PheXaa: 0.0 ± 0.0
Gly
11.171GlyAla: 11.171 ± 0.081
0.909GlyCys: 0.909 ± 0.024
5.786GlyAsp: 5.786 ± 0.055
5.395GlyGlu: 5.395 ± 0.058
2.996GlyPhe: 2.996 ± 0.037
9.526GlyGly: 9.526 ± 0.1
2.217GlyHis: 2.217 ± 0.031
3.25GlyIle: 3.25 ± 0.041
2.162GlyLys: 2.162 ± 0.035
9.401GlyLeu: 9.401 ± 0.08
2.211GlyMet: 2.211 ± 0.038
1.603GlyAsn: 1.603 ± 0.029
5.516GlyPro: 5.516 ± 0.056
2.198GlyGln: 2.198 ± 0.039
9.26GlyArg: 9.26 ± 0.08
4.813GlySer: 4.813 ± 0.053
6.209GlyThr: 6.209 ± 0.061
7.794GlyVal: 7.794 ± 0.076
1.769GlyTrp: 1.769 ± 0.031
2.172GlyTyr: 2.172 ± 0.034
0.0GlyXaa: 0.0 ± 0.0
His
2.641HisAla: 2.641 ± 0.04
0.203HisCys: 0.203 ± 0.009
1.381HisAsp: 1.381 ± 0.027
1.17HisGlu: 1.17 ± 0.021
0.607HisPhe: 0.607 ± 0.019
2.426HisGly: 2.426 ± 0.031
0.62HisHis: 0.62 ± 0.02
0.661HisIle: 0.661 ± 0.02
0.284HisLys: 0.284 ± 0.012
2.294HisLeu: 2.294 ± 0.034
0.384HisMet: 0.384 ± 0.014
0.341HisAsn: 0.341 ± 0.013
1.667HisPro: 1.667 ± 0.023
0.483HisGln: 0.483 ± 0.016
2.217HisArg: 2.217 ± 0.038
0.854HisSer: 0.854 ± 0.02
1.027HisThr: 1.027 ± 0.022
1.799HisVal: 1.799 ± 0.032
0.346HisTrp: 0.346 ± 0.013
0.496HisTyr: 0.496 ± 0.014
0.0HisXaa: 0.0 ± 0.0
Ile
5.189IleAla: 5.189 ± 0.059
0.3IleCys: 0.3 ± 0.011
2.478IleAsp: 2.478 ± 0.038
2.218IleGlu: 2.218 ± 0.031
0.767IlePhe: 0.767 ± 0.022
3.803IleGly: 3.803 ± 0.047
0.529IleHis: 0.529 ± 0.017
0.978IleIle: 0.978 ± 0.024
0.669IleLys: 0.669 ± 0.018
2.437IleLeu: 2.437 ± 0.034
0.538IleMet: 0.538 ± 0.016
0.653IleAsn: 0.653 ± 0.022
1.749IlePro: 1.749 ± 0.034
0.576IleGln: 0.576 ± 0.019
2.321IleArg: 2.321 ± 0.034
1.574IleSer: 1.574 ± 0.029
2.17IleThr: 2.17 ± 0.028
3.431IleVal: 3.431 ± 0.044
0.399IleTrp: 0.399 ± 0.014
0.529IleTyr: 0.529 ± 0.016
0.0IleXaa: 0.0 ± 0.0
Lys
2.487LysAla: 2.487 ± 0.037
0.105LysCys: 0.105 ± 0.007
1.106LysAsp: 1.106 ± 0.026
1.054LysGlu: 1.054 ± 0.024
0.464LysPhe: 0.464 ± 0.017
1.49LysGly: 1.49 ± 0.036
0.398LysHis: 0.398 ± 0.016
0.934LysIle: 0.934 ± 0.026
0.641LysLys: 0.641 ± 0.022
1.738LysLeu: 1.738 ± 0.035
0.408LysMet: 0.408 ± 0.016
0.44LysAsn: 0.44 ± 0.016
1.262LysPro: 1.262 ± 0.028
0.528LysGln: 0.528 ± 0.017
1.437LysArg: 1.437 ± 0.03
1.021LysSer: 1.021 ± 0.027
1.199LysThr: 1.199 ± 0.025
1.724LysVal: 1.724 ± 0.03
0.251LysTrp: 0.251 ± 0.013
0.391LysTyr: 0.391 ± 0.013
0.0LysXaa: 0.0 ± 0.0
Leu
14.926LeuAla: 14.926 ± 0.107
0.755LeuCys: 0.755 ± 0.019
6.98LeuAsp: 6.98 ± 0.061
4.696LeuGlu: 4.696 ± 0.053
2.617LeuPhe: 2.617 ± 0.043
9.031LeuGly: 9.031 ± 0.077
2.133LeuHis: 2.133 ± 0.032
3.343LeuIle: 3.343 ± 0.046
1.733LeuLys: 1.733 ± 0.034
10.607LeuLeu: 10.607 ± 0.103
1.598LeuMet: 1.598 ± 0.031
1.726LeuAsn: 1.726 ± 0.027
6.056LeuPro: 6.056 ± 0.063
2.07LeuGln: 2.07 ± 0.034
8.815LeuArg: 8.815 ± 0.073
4.608LeuSer: 4.608 ± 0.056
6.172LeuThr: 6.172 ± 0.053
8.6LeuVal: 8.6 ± 0.076
1.237LeuTrp: 1.237 ± 0.024
1.752LeuTyr: 1.752 ± 0.028
0.0LeuXaa: 0.0 ± 0.0
Met
2.35MetAla: 2.35 ± 0.036
0.154MetCys: 0.154 ± 0.009
1.023MetAsp: 1.023 ± 0.024
0.842MetGlu: 0.842 ± 0.022
0.582MetPhe: 0.582 ± 0.016
1.391MetGly: 1.391 ± 0.026
0.378MetHis: 0.378 ± 0.012
0.822MetIle: 0.822 ± 0.022
0.432MetLys: 0.432 ± 0.016
1.979MetLeu: 1.979 ± 0.031
0.351MetMet: 0.351 ± 0.014
0.482MetAsn: 0.482 ± 0.013
1.263MetPro: 1.263 ± 0.027
0.475MetGln: 0.475 ± 0.013
1.761MetArg: 1.761 ± 0.032
1.366MetSer: 1.366 ± 0.024
1.667MetThr: 1.667 ± 0.032
1.355MetVal: 1.355 ± 0.028
0.238MetTrp: 0.238 ± 0.01
0.322MetTyr: 0.322 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
2.15AsnAla: 2.15 ± 0.032
0.172AsnCys: 0.172 ± 0.009
0.93AsnAsp: 0.93 ± 0.024
0.89AsnGlu: 0.89 ± 0.024
0.431AsnPhe: 0.431 ± 0.014
1.845AsnGly: 1.845 ± 0.032
0.361AsnHis: 0.361 ± 0.013
0.588AsnIle: 0.588 ± 0.017
0.326AsnLys: 0.326 ± 0.013
1.712AsnLeu: 1.712 ± 0.033
0.313AsnMet: 0.313 ± 0.012
0.345AsnAsn: 0.345 ± 0.012
1.292AsnPro: 1.292 ± 0.028
0.406AsnGln: 0.406 ± 0.016
1.336AsnArg: 1.336 ± 0.025
0.74AsnSer: 0.74 ± 0.019
1.015AsnThr: 1.015 ± 0.024
1.519AsnVal: 1.519 ± 0.029
0.282AsnTrp: 0.282 ± 0.011
0.366AsnTyr: 0.366 ± 0.014
0.0AsnXaa: 0.0 ± 0.0
Pro
8.511ProAla: 8.511 ± 0.069
0.402ProCys: 0.402 ± 0.016
5.172ProAsp: 5.172 ± 0.057
4.266ProGlu: 4.266 ± 0.05
1.662ProPhe: 1.662 ± 0.029
7.237ProGly: 7.237 ± 0.077
1.371ProHis: 1.371 ± 0.03
1.616ProIle: 1.616 ± 0.028
1.167ProLys: 1.167 ± 0.027
5.038ProLeu: 5.038 ± 0.054
1.181ProMet: 1.181 ± 0.024
1.056ProAsn: 1.056 ± 0.028
4.335ProPro: 4.335 ± 0.059
1.386ProGln: 1.386 ± 0.025
4.65ProArg: 4.65 ± 0.051
3.487ProSer: 3.487 ± 0.052
2.934ProThr: 2.934 ± 0.038
5.344ProVal: 5.344 ± 0.053
0.944ProTrp: 0.944 ± 0.021
1.254ProTyr: 1.254 ± 0.026
0.0ProXaa: 0.0 ± 0.0
Gln
3.099GlnAla: 3.099 ± 0.043
0.145GlnCys: 0.145 ± 0.007
1.251GlnAsp: 1.251 ± 0.026
1.208GlnGlu: 1.208 ± 0.026
0.657GlnPhe: 0.657 ± 0.019
1.889GlnGly: 1.889 ± 0.03
0.518GlnHis: 0.518 ± 0.018
1.06GlnIle: 1.06 ± 0.022
0.524GlnLys: 0.524 ± 0.015
2.112GlnLeu: 2.112 ± 0.039
0.484GlnMet: 0.484 ± 0.017
0.503GlnAsn: 0.503 ± 0.017
1.303GlnPro: 1.303 ± 0.031
0.903GlnGln: 0.903 ± 0.03
2.125GlnArg: 2.125 ± 0.034
1.019GlnSer: 1.019 ± 0.022
1.213GlnThr: 1.213 ± 0.025
2.139GlnVal: 2.139 ± 0.03
0.387GlnTrp: 0.387 ± 0.014
0.479GlnTyr: 0.479 ± 0.018
0.0GlnXaa: 0.0 ± 0.0
Arg
11.168ArgAla: 11.168 ± 0.089
0.783ArgCys: 0.783 ± 0.021
5.398ArgAsp: 5.398 ± 0.057
5.022ArgGlu: 5.022 ± 0.061
2.608ArgPhe: 2.608 ± 0.034
6.474ArgGly: 6.474 ± 0.058
2.231ArgHis: 2.231 ± 0.033
3.24ArgIle: 3.24 ± 0.045
1.59ArgLys: 1.59 ± 0.029
9.473ArgLeu: 9.473 ± 0.082
2.077ArgMet: 2.077 ± 0.03
1.404ArgAsn: 1.404 ± 0.026
6.219ArgPro: 6.219 ± 0.065
2.003ArgGln: 2.003 ± 0.037
9.759ArgArg: 9.759 ± 0.108
4.306ArgSer: 4.306 ± 0.055
5.664ArgThr: 5.664 ± 0.064
6.686ArgVal: 6.686 ± 0.058
1.543ArgTrp: 1.543 ± 0.029
1.919ArgTyr: 1.919 ± 0.031
0.0ArgXaa: 0.0 ± 0.0
Ser
6.107SerAla: 6.107 ± 0.055
0.447SerCys: 0.447 ± 0.017
2.533SerAsp: 2.533 ± 0.037
2.145SerGlu: 2.145 ± 0.038
1.452SerPhe: 1.452 ± 0.026
5.774SerGly: 5.774 ± 0.058
0.849SerHis: 0.849 ± 0.02
1.552SerIle: 1.552 ± 0.03
0.941SerLys: 0.941 ± 0.021
4.274SerLeu: 4.274 ± 0.048
1.197SerMet: 1.197 ± 0.024
0.795SerAsn: 0.795 ± 0.019
3.328SerPro: 3.328 ± 0.045
0.972SerGln: 0.972 ± 0.024
3.969SerArg: 3.969 ± 0.056
2.826SerSer: 2.826 ± 0.056
2.723SerThr: 2.723 ± 0.04
3.795SerVal: 3.795 ± 0.048
0.867SerTrp: 0.867 ± 0.021
1.019SerTyr: 1.019 ± 0.022
0.0SerXaa: 0.0 ± 0.0
Thr
7.984ThrAla: 7.984 ± 0.075
0.5ThrCys: 0.5 ± 0.016
3.331ThrAsp: 3.331 ± 0.046
2.878ThrGlu: 2.878 ± 0.041
1.739ThrPhe: 1.739 ± 0.028
6.616ThrGly: 6.616 ± 0.054
0.996ThrHis: 0.996 ± 0.022
2.041ThrIle: 2.041 ± 0.031
1.012ThrLys: 1.012 ± 0.026
5.339ThrLeu: 5.339 ± 0.053
1.14ThrMet: 1.14 ± 0.022
0.93ThrAsn: 0.93 ± 0.021
3.733ThrPro: 3.733 ± 0.042
0.989ThrGln: 0.989 ± 0.021
4.173ThrArg: 4.173 ± 0.05
3.012ThrSer: 3.012 ± 0.044
3.335ThrThr: 3.335 ± 0.053
5.665ThrVal: 5.665 ± 0.053
0.966ThrTrp: 0.966 ± 0.021
1.145ThrTyr: 1.145 ± 0.027
0.0ThrXaa: 0.0 ± 0.0
Val
10.959ValAla: 10.959 ± 0.087
0.704ValCys: 0.704 ± 0.021
5.01ValAsp: 5.01 ± 0.051
4.886ValGlu: 4.886 ± 0.051
2.505ValPhe: 2.505 ± 0.036
6.598ValGly: 6.598 ± 0.066
2.005ValHis: 2.005 ± 0.032
2.867ValIle: 2.867 ± 0.04
1.519ValLys: 1.519 ± 0.028
9.441ValLeu: 9.441 ± 0.074
1.437ValMet: 1.437 ± 0.029
1.603ValAsn: 1.603 ± 0.033
5.692ValPro: 5.692 ± 0.067
2.049ValGln: 2.049 ± 0.034
8.134ValArg: 8.134 ± 0.073
3.978ValSer: 3.978 ± 0.044
5.323ValThr: 5.323 ± 0.055
7.683ValVal: 7.683 ± 0.073
1.135ValTrp: 1.135 ± 0.026
1.585ValTyr: 1.585 ± 0.028
0.0ValXaa: 0.0 ± 0.0
Trp
1.697TrpAla: 1.697 ± 0.028
0.189TrpCys: 0.189 ± 0.01
0.909TrpAsp: 0.909 ± 0.027
0.739TrpGlu: 0.739 ± 0.018
0.528TrpPhe: 0.528 ± 0.015
1.052TrpGly: 1.052 ± 0.021
0.354TrpHis: 0.354 ± 0.014
0.597TrpIle: 0.597 ± 0.018
0.342TrpLys: 0.342 ± 0.014
1.692TrpLeu: 1.692 ± 0.031
0.289TrpMet: 0.289 ± 0.01
0.383TrpAsn: 0.383 ± 0.012
0.864TrpPro: 0.864 ± 0.017
0.448TrpGln: 0.448 ± 0.013
1.545TrpArg: 1.545 ± 0.032
0.893TrpSer: 0.893 ± 0.022
1.222TrpThr: 1.222 ± 0.026
0.937TrpVal: 0.937 ± 0.023
0.35TrpTrp: 0.35 ± 0.013
0.338TrpTyr: 0.338 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.502TyrAla: 2.502 ± 0.031
0.183TyrCys: 0.183 ± 0.009
1.297TyrAsp: 1.297 ± 0.03
1.143TyrGlu: 1.143 ± 0.025
0.61TyrPhe: 0.61 ± 0.019
2.141TyrGly: 2.141 ± 0.032
0.394TyrHis: 0.394 ± 0.014
0.536TyrIle: 0.536 ± 0.016
0.365TyrLys: 0.365 ± 0.014
2.056TyrLeu: 2.056 ± 0.033
0.304TyrMet: 0.304 ± 0.013
0.355TyrAsn: 0.355 ± 0.014
1.049TyrPro: 1.049 ± 0.023
0.477TyrGln: 0.477 ± 0.015
1.961TyrArg: 1.961 ± 0.031
0.832TyrSer: 0.832 ± 0.021
1.094TyrThr: 1.094 ± 0.025
1.597TyrVal: 1.597 ± 0.029
0.367TyrTrp: 0.367 ± 0.015
0.455TyrTyr: 0.455 ± 0.016
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7656 proteins (2085195 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski