Proteome-pI 2.0 - Proteome Isoelectric Point Database

Beihai narna-like virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A1L3KIC8|A0A1L3KIC8_9VIRU RNA-dependent RNA polymerase OS=Beihai narna-like virus 2 OX=1922447 PE=4 SV=1

MM1 pKa = 7.15EE2 pKa = 4.13TTLAKK7 pKa = 10.56ALVEE11 pKa = 4.36SCVKK15 pKa = 10.61YY16 pKa = 9.99YY17 pKa = 10.66SPKK20 pKa = 9.48EE21 pKa = 3.55ASAYY25 pKa = 10.44LIEE28 pKa = 5.07FGVKK32 pKa = 9.56YY33 pKa = 10.33FGLSSCHH40 pKa = 7.35SPDD43 pKa = 3.51LWDD46 pKa = 3.6EE47 pKa = 4.1FLGRR51 pKa = 11.84FYY53 pKa = 10.47PSSDD57 pKa = 4.38SIPMDD62 pKa = 3.4GVTDD66 pKa = 3.5WRR68 pKa = 11.84SDD70 pKa = 3.36LSRR73 pKa = 11.84EE74 pKa = 4.04IVDD77 pKa = 3.44SYY79 pKa = 11.62EE80 pKa = 3.68KK81 pKa = 9.82VKK83 pKa = 10.94RR84 pKa = 11.84MFEE87 pKa = 4.1VYY89 pKa = 10.27SYY91 pKa = 10.88IINTRR96 pKa = 11.84LGQTLTWASQRR107 pKa = 11.84EE108 pKa = 4.41TMTRR112 pKa = 11.84WFRR115 pKa = 11.84YY116 pKa = 8.97ALRR119 pKa = 11.84GKK121 pKa = 9.92LEE123 pKa = 4.0KK124 pKa = 10.52VLKK127 pKa = 10.54FKK129 pKa = 10.02TATLLALGLNQSEE142 pKa = 4.46LPPRR146 pKa = 11.84PSCLEE151 pKa = 3.73ADD153 pKa = 3.77DD154 pKa = 4.06RR155 pKa = 11.84ANYY158 pKa = 9.57LFQGKK163 pKa = 8.39VGRR166 pKa = 11.84RR167 pKa = 11.84ILHH170 pKa = 5.39CTRR173 pKa = 11.84IVSAKK178 pKa = 9.28RR179 pKa = 11.84DD180 pKa = 3.43NGEE183 pKa = 3.83RR184 pKa = 11.84VQFLYY189 pKa = 10.75EE190 pKa = 4.25LYY192 pKa = 9.27SAKK195 pKa = 10.14RR196 pKa = 11.84ASLPVSSDD204 pKa = 3.18FVTEE208 pKa = 3.83ALEE211 pKa = 4.24KK212 pKa = 10.46HH213 pKa = 5.53WFTLTEE219 pKa = 4.65LDD221 pKa = 5.51QKK223 pKa = 11.61DD224 pKa = 4.15LDD226 pKa = 4.36NPFLDD231 pKa = 4.66DD232 pKa = 5.23LIEE235 pKa = 4.33EE236 pKa = 4.44VTRR239 pKa = 11.84TVDD242 pKa = 3.44EE243 pKa = 5.3LIPPVKK249 pKa = 9.63CTPHH253 pKa = 6.42YY254 pKa = 9.92KK255 pKa = 9.59VCPSFGASFKK265 pKa = 10.16TGRR268 pKa = 11.84DD269 pKa = 2.91KK270 pKa = 11.52GGSYY274 pKa = 10.62SEE276 pKa = 4.73LVGEE280 pKa = 4.51SQLTSNEE287 pKa = 3.53GHH289 pKa = 7.15LGGFMQKK296 pKa = 9.56SCPILVQEE304 pKa = 4.24SFDD307 pKa = 3.52WKK309 pKa = 11.16SEE311 pKa = 3.94ITSKK315 pKa = 10.3PSKK318 pKa = 10.34IGGGDD323 pKa = 3.33VMGVEE328 pKa = 4.15WRR330 pKa = 11.84VPDD333 pKa = 4.78VDD335 pKa = 5.24EE336 pKa = 5.23DD337 pKa = 3.47WLDD340 pKa = 3.82YY341 pKa = 11.47VSVASQLQDD350 pKa = 2.86IDD352 pKa = 4.08CRR354 pKa = 11.84PVALLEE360 pKa = 4.09PFKK363 pKa = 11.27VRR365 pKa = 11.84VITRR369 pKa = 11.84GSAPAYY375 pKa = 9.3QLARR379 pKa = 11.84AYY381 pKa = 10.39QRR383 pKa = 11.84IIAPLVGKK391 pKa = 10.16NPAFRR396 pKa = 11.84LTRR399 pKa = 11.84GPAKK403 pKa = 9.4MDD405 pKa = 4.56HH406 pKa = 6.75INDD409 pKa = 4.27LLEE412 pKa = 4.38KK413 pKa = 10.27AQGPSAEE420 pKa = 4.18YY421 pKa = 10.34IEE423 pKa = 5.5AFEE426 pKa = 4.45CGARR430 pKa = 11.84RR431 pKa = 11.84STLEE435 pKa = 3.94AEE437 pKa = 5.07DD438 pKa = 4.39YY439 pKa = 11.44SSGTWRR445 pKa = 11.84PEE447 pKa = 4.3GIWVSGDD454 pKa = 3.41YY455 pKa = 10.96EE456 pKa = 4.57SATDD460 pKa = 4.49LLNATLSEE468 pKa = 4.05ICLDD472 pKa = 4.5RR473 pKa = 11.84ILHH476 pKa = 6.01NLDD479 pKa = 2.92APYY482 pKa = 10.64EE483 pKa = 4.06HH484 pKa = 7.26RR485 pKa = 11.84RR486 pKa = 11.84TLFGCLTRR494 pKa = 11.84HH495 pKa = 5.65TLHH498 pKa = 6.77SADD501 pKa = 4.21DD502 pKa = 4.13SKK504 pKa = 11.58VAPQINGQLMGSPLSFPILCMVNAAVTRR532 pKa = 11.84KK533 pKa = 10.09AIEE536 pKa = 3.97VCTGRR541 pKa = 11.84KK542 pKa = 9.19KK543 pKa = 10.62LLKK546 pKa = 10.32DD547 pKa = 3.1HH548 pKa = 7.0AMLINGDD555 pKa = 3.72DD556 pKa = 3.67VLFHH560 pKa = 6.53LRR562 pKa = 11.84SIAEE566 pKa = 4.02YY567 pKa = 9.79EE568 pKa = 4.01VWKK571 pKa = 9.22TFTAGAGLKK580 pKa = 10.42FSLGKK585 pKa = 10.39NYY587 pKa = 9.94VSRR590 pKa = 11.84SYY592 pKa = 11.22LVINSEE598 pKa = 3.82IFKK601 pKa = 10.35IRR603 pKa = 11.84PHH605 pKa = 6.94VNFFGEE611 pKa = 4.49RR612 pKa = 11.84KK613 pKa = 8.82WICDD617 pKa = 3.05RR618 pKa = 11.84SLPIINMGHH627 pKa = 6.24VYY629 pKa = 9.36VQKK632 pKa = 10.65KK633 pKa = 10.24SSSKK637 pKa = 10.11LQSEE641 pKa = 3.93QSMYY645 pKa = 8.64GTHH648 pKa = 6.46YY649 pKa = 10.02LQKK652 pKa = 10.99DD653 pKa = 3.76SLKK656 pKa = 10.78DD657 pKa = 3.32SCEE660 pKa = 4.35DD661 pKa = 3.24FVEE664 pKa = 4.07AHH666 pKa = 6.86PLDD669 pKa = 3.93KK670 pKa = 10.92RR671 pKa = 11.84CFALSKK677 pKa = 9.55WISARR682 pKa = 11.84KK683 pKa = 9.55HH684 pKa = 5.97EE685 pKa = 5.87LDD687 pKa = 3.08TCLPKK692 pKa = 10.67GMSYY696 pKa = 10.32FVHH699 pKa = 6.83EE700 pKa = 4.51SLGGLGLPVYY710 pKa = 8.6PHH712 pKa = 7.41KK713 pKa = 10.88DD714 pKa = 3.14QLVGITEE721 pKa = 4.41GQRR724 pKa = 11.84KK725 pKa = 7.99LAAYY729 pKa = 9.33LWTSQDD735 pKa = 2.89PTARR739 pKa = 11.84LINTGALRR747 pKa = 11.84LPIPSFVEE755 pKa = 4.1RR756 pKa = 11.84YY757 pKa = 8.85MRR759 pKa = 11.84DD760 pKa = 3.02KK761 pKa = 11.41SDD763 pKa = 3.15LLQRR767 pKa = 11.84LDD769 pKa = 4.72VRR771 pKa = 11.84YY772 pKa = 8.49EE773 pKa = 3.63WSNVKK778 pKa = 10.1VKK780 pKa = 10.63EE781 pKa = 4.08DD782 pKa = 3.17WPDD785 pKa = 3.42FHH787 pKa = 7.41RR788 pKa = 11.84WTAVGIVGQDD798 pKa = 3.55PPDD801 pKa = 3.46MKK803 pKa = 10.39PFRR806 pKa = 11.84RR807 pKa = 11.84VYY809 pKa = 9.03EE810 pKa = 4.1AVWRR814 pKa = 11.84DD815 pKa = 3.62AQASWASPLSFRR827 pKa = 11.84KK828 pKa = 9.81CIKK831 pKa = 10.45GVFDD835 pKa = 3.96GVPGYY840 pKa = 9.98FGPAEE845 pKa = 3.98RR846 pKa = 11.84VRR848 pKa = 11.84KK849 pKa = 8.85QQ850 pKa = 2.84

Molecular weight: 96.65 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A1L3KIC8|A0A1L3KIC8_9VIRU RNA-dependent RNA polymerase OS=Beihai narna-like virus 2 OX=1922447 PE=4 SV=1

MM1 pKa = 7.15EE2 pKa = 4.13TTLAKK7 pKa = 10.56ALVEE11 pKa = 4.36SCVKK15 pKa = 10.61YY16 pKa = 9.99YY17 pKa = 10.66SPKK20 pKa = 9.48EE21 pKa = 3.55ASAYY25 pKa = 10.44LIEE28 pKa = 5.07FGVKK32 pKa = 9.56YY33 pKa = 10.33FGLSSCHH40 pKa = 7.35SPDD43 pKa = 3.51LWDD46 pKa = 3.6EE47 pKa = 4.1FLGRR51 pKa = 11.84FYY53 pKa = 10.47PSSDD57 pKa = 4.38SIPMDD62 pKa = 3.4GVTDD66 pKa = 3.5WRR68 pKa = 11.84SDD70 pKa = 3.36LSRR73 pKa = 11.84EE74 pKa = 4.04IVDD77 pKa = 3.44SYY79 pKa = 11.62EE80 pKa = 3.68KK81 pKa = 9.82VKK83 pKa = 10.94RR84 pKa = 11.84MFEE87 pKa = 4.1VYY89 pKa = 10.27SYY91 pKa = 10.88IINTRR96 pKa = 11.84LGQTLTWASQRR107 pKa = 11.84EE108 pKa = 4.41TMTRR112 pKa = 11.84WFRR115 pKa = 11.84YY116 pKa = 8.97ALRR119 pKa = 11.84GKK121 pKa = 9.92LEE123 pKa = 4.0KK124 pKa = 10.52VLKK127 pKa = 10.54FKK129 pKa = 10.02TATLLALGLNQSEE142 pKa = 4.46LPPRR146 pKa = 11.84PSCLEE151 pKa = 3.73ADD153 pKa = 3.77DD154 pKa = 4.06RR155 pKa = 11.84ANYY158 pKa = 9.57LFQGKK163 pKa = 8.39VGRR166 pKa = 11.84RR167 pKa = 11.84ILHH170 pKa = 5.39CTRR173 pKa = 11.84IVSAKK178 pKa = 9.28RR179 pKa = 11.84DD180 pKa = 3.43NGEE183 pKa = 3.83RR184 pKa = 11.84VQFLYY189 pKa = 10.75EE190 pKa = 4.25LYY192 pKa = 9.27SAKK195 pKa = 10.14RR196 pKa = 11.84ASLPVSSDD204 pKa = 3.18FVTEE208 pKa = 3.83ALEE211 pKa = 4.24KK212 pKa = 10.46HH213 pKa = 5.53WFTLTEE219 pKa = 4.65LDD221 pKa = 5.51QKK223 pKa = 11.61DD224 pKa = 4.15LDD226 pKa = 4.36NPFLDD231 pKa = 4.66DD232 pKa = 5.23LIEE235 pKa = 4.33EE236 pKa = 4.44VTRR239 pKa = 11.84TVDD242 pKa = 3.44EE243 pKa = 5.3LIPPVKK249 pKa = 9.63CTPHH253 pKa = 6.42YY254 pKa = 9.92KK255 pKa = 9.59VCPSFGASFKK265 pKa = 10.16TGRR268 pKa = 11.84DD269 pKa = 2.91KK270 pKa = 11.52GGSYY274 pKa = 10.62SEE276 pKa = 4.73LVGEE280 pKa = 4.51SQLTSNEE287 pKa = 3.53GHH289 pKa = 7.15LGGFMQKK296 pKa = 9.56SCPILVQEE304 pKa = 4.24SFDD307 pKa = 3.52WKK309 pKa = 11.16SEE311 pKa = 3.94ITSKK315 pKa = 10.3PSKK318 pKa = 10.34IGGGDD323 pKa = 3.33VMGVEE328 pKa = 4.15WRR330 pKa = 11.84VPDD333 pKa = 4.78VDD335 pKa = 5.24EE336 pKa = 5.23DD337 pKa = 3.47WLDD340 pKa = 3.82YY341 pKa = 11.47VSVASQLQDD350 pKa = 2.86IDD352 pKa = 4.08CRR354 pKa = 11.84PVALLEE360 pKa = 4.09PFKK363 pKa = 11.27VRR365 pKa = 11.84VITRR369 pKa = 11.84GSAPAYY375 pKa = 9.3QLARR379 pKa = 11.84AYY381 pKa = 10.39QRR383 pKa = 11.84IIAPLVGKK391 pKa = 10.16NPAFRR396 pKa = 11.84LTRR399 pKa = 11.84GPAKK403 pKa = 9.4MDD405 pKa = 4.56HH406 pKa = 6.75INDD409 pKa = 4.27LLEE412 pKa = 4.38KK413 pKa = 10.27AQGPSAEE420 pKa = 4.18YY421 pKa = 10.34IEE423 pKa = 5.5AFEE426 pKa = 4.45CGARR430 pKa = 11.84RR431 pKa = 11.84STLEE435 pKa = 3.94AEE437 pKa = 5.07DD438 pKa = 4.39YY439 pKa = 11.44SSGTWRR445 pKa = 11.84PEE447 pKa = 4.3GIWVSGDD454 pKa = 3.41YY455 pKa = 10.96EE456 pKa = 4.57SATDD460 pKa = 4.49LLNATLSEE468 pKa = 4.05ICLDD472 pKa = 4.5RR473 pKa = 11.84ILHH476 pKa = 6.01NLDD479 pKa = 2.92APYY482 pKa = 10.64EE483 pKa = 4.06HH484 pKa = 7.26RR485 pKa = 11.84RR486 pKa = 11.84TLFGCLTRR494 pKa = 11.84HH495 pKa = 5.65TLHH498 pKa = 6.77SADD501 pKa = 4.21DD502 pKa = 4.13SKK504 pKa = 11.58VAPQINGQLMGSPLSFPILCMVNAAVTRR532 pKa = 11.84KK533 pKa = 10.09AIEE536 pKa = 3.97VCTGRR541 pKa = 11.84KK542 pKa = 9.19KK543 pKa = 10.62LLKK546 pKa = 10.32DD547 pKa = 3.1HH548 pKa = 7.0AMLINGDD555 pKa = 3.72DD556 pKa = 3.67VLFHH560 pKa = 6.53LRR562 pKa = 11.84SIAEE566 pKa = 4.02YY567 pKa = 9.79EE568 pKa = 4.01VWKK571 pKa = 9.22TFTAGAGLKK580 pKa = 10.42FSLGKK585 pKa = 10.39NYY587 pKa = 9.94VSRR590 pKa = 11.84SYY592 pKa = 11.22LVINSEE598 pKa = 3.82IFKK601 pKa = 10.35IRR603 pKa = 11.84PHH605 pKa = 6.94VNFFGEE611 pKa = 4.49RR612 pKa = 11.84KK613 pKa = 8.82WICDD617 pKa = 3.05RR618 pKa = 11.84SLPIINMGHH627 pKa = 6.24VYY629 pKa = 9.36VQKK632 pKa = 10.65KK633 pKa = 10.24SSSKK637 pKa = 10.11LQSEE641 pKa = 3.93QSMYY645 pKa = 8.64GTHH648 pKa = 6.46YY649 pKa = 10.02LQKK652 pKa = 10.99DD653 pKa = 3.76SLKK656 pKa = 10.78DD657 pKa = 3.32SCEE660 pKa = 4.35DD661 pKa = 3.24FVEE664 pKa = 4.07AHH666 pKa = 6.86PLDD669 pKa = 3.93KK670 pKa = 10.92RR671 pKa = 11.84CFALSKK677 pKa = 9.55WISARR682 pKa = 11.84KK683 pKa = 9.55HH684 pKa = 5.97EE685 pKa = 5.87LDD687 pKa = 3.08TCLPKK692 pKa = 10.67GMSYY696 pKa = 10.32FVHH699 pKa = 6.83EE700 pKa = 4.51SLGGLGLPVYY710 pKa = 8.6PHH712 pKa = 7.41KK713 pKa = 10.88DD714 pKa = 3.14QLVGITEE721 pKa = 4.41GQRR724 pKa = 11.84KK725 pKa = 7.99LAAYY729 pKa = 9.33LWTSQDD735 pKa = 2.89PTARR739 pKa = 11.84LINTGALRR747 pKa = 11.84LPIPSFVEE755 pKa = 4.1RR756 pKa = 11.84YY757 pKa = 8.85MRR759 pKa = 11.84DD760 pKa = 3.02KK761 pKa = 11.41SDD763 pKa = 3.15LLQRR767 pKa = 11.84LDD769 pKa = 4.72VRR771 pKa = 11.84YY772 pKa = 8.49EE773 pKa = 3.63WSNVKK778 pKa = 10.1VKK780 pKa = 10.63EE781 pKa = 4.08DD782 pKa = 3.17WPDD785 pKa = 3.42FHH787 pKa = 7.41RR788 pKa = 11.84WTAVGIVGQDD798 pKa = 3.55PPDD801 pKa = 3.46MKK803 pKa = 10.39PFRR806 pKa = 11.84RR807 pKa = 11.84VYY809 pKa = 9.03EE810 pKa = 4.1AVWRR814 pKa = 11.84DD815 pKa = 3.62AQASWASPLSFRR827 pKa = 11.84KK828 pKa = 9.81CIKK831 pKa = 10.45GVFDD835 pKa = 3.96GVPGYY840 pKa = 9.98FGPAEE845 pKa = 3.98RR846 pKa = 11.84VRR848 pKa = 11.84KK849 pKa = 8.85QQ850 pKa = 2.84

Molecular weight: 96.65 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
1	0	1	850	850	850	850.0	96.65

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
6.353 ± 0.0	2.118 ± 0.0	6.471 ± 0.0	6.353 ± 0.0	4.235 ± 0.0	6.353 ± 0.0	2.353 ± 0.0	4.471 ± 0.0	6.471 ± 0.0	10.0 ± 0.0

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
1.765 ± 0.0	2.235 ± 0.0	5.176 ± 0.0	3.176 ± 0.0	6.706 ± 0.0	8.235 ± 0.0	4.706 ± 0.0	6.588 ± 0.0	2.235 ± 0.0	4.0 ± 0.0

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski