Amino acid dipepetide frequency for Bacillus phage Saddex

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.529AlaAla: 4.529 ± 0.74
0.288AlaCys: 0.288 ± 0.087
3.738AlaAsp: 3.738 ± 0.335
4.553AlaGlu: 4.553 ± 0.328
2.444AlaPhe: 2.444 ± 0.247
3.57AlaGly: 3.57 ± 0.443
1.078AlaHis: 1.078 ± 0.149
4.193AlaIle: 4.193 ± 0.296
5.128AlaLys: 5.128 ± 0.383
5.08AlaLeu: 5.08 ± 0.333
1.869AlaMet: 1.869 ± 0.214
3.546AlaAsn: 3.546 ± 0.345
1.797AlaPro: 1.797 ± 0.218
2.468AlaGln: 2.468 ± 0.25
2.684AlaArg: 2.684 ± 0.245
3.57AlaSer: 3.57 ± 0.408
4.265AlaThr: 4.265 ± 0.444
3.858AlaVal: 3.858 ± 0.298
0.695AlaTrp: 0.695 ± 0.132
2.564AlaTyr: 2.564 ± 0.282
0.0AlaXaa: 0.0 ± 0.0
Cys
0.312CysAla: 0.312 ± 0.091
0.12CysCys: 0.12 ± 0.074
0.503CysAsp: 0.503 ± 0.11
0.743CysGlu: 0.743 ± 0.147
0.383CysPhe: 0.383 ± 0.098
0.623CysGly: 0.623 ± 0.153
0.288CysHis: 0.288 ± 0.086
0.455CysIle: 0.455 ± 0.091
0.719CysLys: 0.719 ± 0.163
0.503CysLeu: 0.503 ± 0.105
0.264CysMet: 0.264 ± 0.087
0.383CysAsn: 0.383 ± 0.117
0.312CysPro: 0.312 ± 0.087
0.192CysGln: 0.192 ± 0.058
0.264CysArg: 0.264 ± 0.093
0.407CysSer: 0.407 ± 0.08
0.671CysThr: 0.671 ± 0.116
0.527CysVal: 0.527 ± 0.11
0.168CysTrp: 0.168 ± 0.072
0.383CysTyr: 0.383 ± 0.101
0.0CysXaa: 0.0 ± 0.0
Asp
4.337AspAla: 4.337 ± 0.351
0.647AspCys: 0.647 ± 0.128
4.361AspAsp: 4.361 ± 0.43
5.272AspGlu: 5.272 ± 0.435
2.804AspPhe: 2.804 ± 0.31
4.169AspGly: 4.169 ± 0.347
0.791AspHis: 0.791 ± 0.153
5.152AspIle: 5.152 ± 0.362
5.871AspLys: 5.871 ± 0.376
4.768AspLeu: 4.768 ± 0.31
2.157AspMet: 2.157 ± 0.231
3.235AspAsn: 3.235 ± 0.272
2.013AspPro: 2.013 ± 0.311
1.078AspGln: 1.078 ± 0.144
2.492AspArg: 2.492 ± 0.204
3.451AspSer: 3.451 ± 0.237
3.906AspThr: 3.906 ± 0.417
5.2AspVal: 5.2 ± 0.357
0.815AspTrp: 0.815 ± 0.152
2.947AspTyr: 2.947 ± 0.288
0.0AspXaa: 0.0 ± 0.0
Glu
4.481GluAla: 4.481 ± 0.381
0.527GluCys: 0.527 ± 0.122
4.577GluAsp: 4.577 ± 0.41
8.818GluGlu: 8.818 ± 0.906
3.666GluPhe: 3.666 ± 0.285
4.768GluGly: 4.768 ± 0.36
1.653GluHis: 1.653 ± 0.226
4.96GluIle: 4.96 ± 0.386
6.158GluLys: 6.158 ± 0.554
7.859GluLeu: 7.859 ± 0.504
2.971GluMet: 2.971 ± 0.25
3.618GluAsn: 3.618 ± 0.34
1.965GluPro: 1.965 ± 0.247
3.379GluGln: 3.379 ± 0.326
2.923GluArg: 2.923 ± 0.289
3.978GluSer: 3.978 ± 0.337
3.403GluThr: 3.403 ± 0.308
6.302GluVal: 6.302 ± 0.595
1.03GluTrp: 1.03 ± 0.181
3.187GluTyr: 3.187 ± 0.309
0.0GluXaa: 0.0 ± 0.0
Phe
2.324PheAla: 2.324 ± 0.224
0.479PheCys: 0.479 ± 0.093
3.091PheAsp: 3.091 ± 0.3
2.468PheGlu: 2.468 ± 0.247
1.773PhePhe: 1.773 ± 0.228
2.157PheGly: 2.157 ± 0.222
0.695PheHis: 0.695 ± 0.142
2.851PheIle: 2.851 ± 0.243
2.875PheLys: 2.875 ± 0.281
3.163PheLeu: 3.163 ± 0.302
1.438PheMet: 1.438 ± 0.193
2.851PheAsn: 2.851 ± 0.319
1.174PhePro: 1.174 ± 0.172
1.174PheGln: 1.174 ± 0.169
1.558PheArg: 1.558 ± 0.203
2.157PheSer: 2.157 ± 0.235
3.403PheThr: 3.403 ± 0.305
2.54PheVal: 2.54 ± 0.257
0.312PheTrp: 0.312 ± 0.085
1.534PheTyr: 1.534 ± 0.192
0.0PheXaa: 0.0 ± 0.0
Gly
3.546GlyAla: 3.546 ± 0.614
0.455GlyCys: 0.455 ± 0.107
3.57GlyAsp: 3.57 ± 0.28
4.72GlyGlu: 4.72 ± 0.358
2.756GlyPhe: 2.756 ± 0.229
5.367GlyGly: 5.367 ± 0.806
0.935GlyHis: 0.935 ± 0.178
3.714GlyIle: 3.714 ± 0.425
5.152GlyLys: 5.152 ± 0.317
3.882GlyLeu: 3.882 ± 0.331
1.917GlyMet: 1.917 ± 0.215
3.427GlyAsn: 3.427 ± 0.411
0.623GlyPro: 0.623 ± 0.091
1.797GlyGln: 1.797 ± 0.297
2.947GlyArg: 2.947 ± 0.225
3.474GlySer: 3.474 ± 0.355
4.553GlyThr: 4.553 ± 0.394
5.463GlyVal: 5.463 ± 0.352
1.006GlyTrp: 1.006 ± 0.148
3.187GlyTyr: 3.187 ± 0.274
0.0GlyXaa: 0.0 ± 0.0
His
0.815HisAla: 0.815 ± 0.145
0.168HisCys: 0.168 ± 0.066
1.126HisAsp: 1.126 ± 0.175
1.246HisGlu: 1.246 ± 0.188
0.743HisPhe: 0.743 ± 0.159
0.815HisGly: 0.815 ± 0.143
0.312HisHis: 0.312 ± 0.099
1.342HisIle: 1.342 ± 0.208
1.534HisLys: 1.534 ± 0.249
1.462HisLeu: 1.462 ± 0.191
0.599HisMet: 0.599 ± 0.12
1.126HisAsn: 1.126 ± 0.159
0.503HisPro: 0.503 ± 0.115
0.527HisGln: 0.527 ± 0.124
0.791HisArg: 0.791 ± 0.13
0.982HisSer: 0.982 ± 0.176
1.054HisThr: 1.054 ± 0.162
1.653HisVal: 1.653 ± 0.251
0.335HisTrp: 0.335 ± 0.081
0.958HisTyr: 0.958 ± 0.175
0.0HisXaa: 0.0 ± 0.0
Ile
3.93IleAla: 3.93 ± 0.282
0.623IleCys: 0.623 ± 0.145
5.152IleAsp: 5.152 ± 0.296
5.871IleGlu: 5.871 ± 0.367
1.605IlePhe: 1.605 ± 0.187
3.834IleGly: 3.834 ± 0.345
0.958IleHis: 0.958 ± 0.165
3.714IleIle: 3.714 ± 0.301
5.847IleLys: 5.847 ± 0.354
4.457IleLeu: 4.457 ± 0.348
1.941IleMet: 1.941 ± 0.216
3.522IleAsn: 3.522 ± 0.32
1.989IlePro: 1.989 ± 0.254
2.013IleGln: 2.013 ± 0.208
3.163IleArg: 3.163 ± 0.284
4.217IleSer: 4.217 ± 0.324
4.625IleThr: 4.625 ± 0.4
4.361IleVal: 4.361 ± 0.358
0.671IleTrp: 0.671 ± 0.145
2.228IleTyr: 2.228 ± 0.258
0.0IleXaa: 0.0 ± 0.0
Lys
4.816LysAla: 4.816 ± 0.454
0.599LysCys: 0.599 ± 0.131
5.511LysAsp: 5.511 ± 0.342
7.62LysGlu: 7.62 ± 0.547
2.756LysPhe: 2.756 ± 0.312
5.008LysGly: 5.008 ± 0.394
1.749LysHis: 1.749 ± 0.239
3.978LysIle: 3.978 ± 0.326
5.823LysLys: 5.823 ± 0.54
6.59LysLeu: 6.59 ± 0.47
2.612LysMet: 2.612 ± 0.253
4.074LysAsn: 4.074 ± 0.351
2.54LysPro: 2.54 ± 0.284
3.427LysGln: 3.427 ± 0.299
3.283LysArg: 3.283 ± 0.296
3.666LysSer: 3.666 ± 0.374
4.529LysThr: 4.529 ± 0.359
5.655LysVal: 5.655 ± 0.396
1.126LysTrp: 1.126 ± 0.166
3.211LysTyr: 3.211 ± 0.327
0.0LysXaa: 0.0 ± 0.0
Leu
5.056LeuAla: 5.056 ± 0.373
0.599LeuCys: 0.599 ± 0.157
5.655LeuAsp: 5.655 ± 0.373
7.236LeuGlu: 7.236 ± 0.599
2.684LeuPhe: 2.684 ± 0.259
4.864LeuGly: 4.864 ± 0.393
1.15LeuHis: 1.15 ± 0.161
4.505LeuIle: 4.505 ± 0.337
5.535LeuLys: 5.535 ± 0.37
6.206LeuLeu: 6.206 ± 0.411
2.204LeuMet: 2.204 ± 0.243
4.241LeuAsn: 4.241 ± 0.344
2.923LeuPro: 2.923 ± 0.266
3.043LeuGln: 3.043 ± 0.31
3.978LeuArg: 3.978 ± 0.357
5.008LeuSer: 5.008 ± 0.347
5.152LeuThr: 5.152 ± 0.361
5.32LeuVal: 5.32 ± 0.415
0.839LeuTrp: 0.839 ± 0.141
2.78LeuTyr: 2.78 ± 0.355
0.0LeuXaa: 0.0 ± 0.0
Met
1.725MetAla: 1.725 ± 0.207
0.24MetCys: 0.24 ± 0.08
1.989MetAsp: 1.989 ± 0.265
2.276MetGlu: 2.276 ± 0.253
1.294MetPhe: 1.294 ± 0.163
1.629MetGly: 1.629 ± 0.256
0.599MetHis: 0.599 ± 0.126
1.821MetIle: 1.821 ± 0.215
2.78MetLys: 2.78 ± 0.289
2.54MetLeu: 2.54 ± 0.263
0.982MetMet: 0.982 ± 0.203
1.701MetAsn: 1.701 ± 0.187
0.887MetPro: 0.887 ± 0.157
0.791MetGln: 0.791 ± 0.143
1.773MetArg: 1.773 ± 0.246
2.468MetSer: 2.468 ± 0.241
2.396MetThr: 2.396 ± 0.286
1.534MetVal: 1.534 ± 0.203
0.407MetTrp: 0.407 ± 0.108
1.581MetTyr: 1.581 ± 0.231
0.0MetXaa: 0.0 ± 0.0
Asn
3.331AsnAla: 3.331 ± 0.322
0.407AsnCys: 0.407 ± 0.13
2.732AsnAsp: 2.732 ± 0.259
2.995AsnGlu: 2.995 ± 0.243
1.917AsnPhe: 1.917 ± 0.216
4.481AsnGly: 4.481 ± 0.351
1.078AsnHis: 1.078 ± 0.162
3.858AsnIle: 3.858 ± 0.303
4.625AsnLys: 4.625 ± 0.338
4.05AsnLeu: 4.05 ± 0.357
2.133AsnMet: 2.133 ± 0.232
3.498AsnAsn: 3.498 ± 0.367
2.3AsnPro: 2.3 ± 0.313
1.677AsnGln: 1.677 ± 0.201
2.827AsnArg: 2.827 ± 0.267
2.564AsnSer: 2.564 ± 0.3
3.307AsnThr: 3.307 ± 0.276
3.522AsnVal: 3.522 ± 0.323
0.623AsnTrp: 0.623 ± 0.128
2.276AsnTyr: 2.276 ± 0.219
0.0AsnXaa: 0.0 ± 0.0
Pro
2.109ProAla: 2.109 ± 0.334
0.24ProCys: 0.24 ± 0.084
2.276ProAsp: 2.276 ± 0.321
2.66ProGlu: 2.66 ± 0.278
1.51ProPhe: 1.51 ± 0.208
0.024ProGly: 0.024 ± 0.021
0.671ProHis: 0.671 ± 0.123
1.605ProIle: 1.605 ± 0.254
2.468ProLys: 2.468 ± 0.29
1.989ProLeu: 1.989 ± 0.231
0.791ProMet: 0.791 ± 0.157
1.869ProAsn: 1.869 ± 0.242
0.815ProPro: 0.815 ± 0.126
0.911ProGln: 0.911 ± 0.172
1.486ProArg: 1.486 ± 0.192
2.013ProSer: 2.013 ± 0.321
2.564ProThr: 2.564 ± 0.298
2.061ProVal: 2.061 ± 0.242
0.216ProTrp: 0.216 ± 0.068
1.534ProTyr: 1.534 ± 0.184
0.0ProXaa: 0.0 ± 0.0
Gln
2.372GlnAla: 2.372 ± 0.315
0.24GlnCys: 0.24 ± 0.085
2.013GlnAsp: 2.013 ± 0.207
2.636GlnGlu: 2.636 ± 0.242
1.246GlnPhe: 1.246 ± 0.178
2.013GlnGly: 2.013 ± 0.213
0.743GlnHis: 0.743 ± 0.131
2.013GlnIle: 2.013 ± 0.231
1.989GlnLys: 1.989 ± 0.23
3.259GlnLeu: 3.259 ± 0.263
0.958GlnMet: 0.958 ± 0.159
1.653GlnAsn: 1.653 ± 0.178
0.982GlnPro: 0.982 ± 0.143
1.438GlnGln: 1.438 ± 0.222
1.749GlnArg: 1.749 ± 0.229
2.037GlnSer: 2.037 ± 0.245
1.845GlnThr: 1.845 ± 0.192
2.085GlnVal: 2.085 ± 0.254
0.383GlnTrp: 0.383 ± 0.093
1.486GlnTyr: 1.486 ± 0.181
0.0GlnXaa: 0.0 ± 0.0
Arg
2.851ArgAla: 2.851 ± 0.307
0.407ArgCys: 0.407 ± 0.1
2.851ArgAsp: 2.851 ± 0.301
3.642ArgGlu: 3.642 ± 0.382
1.893ArgPhe: 1.893 ± 0.224
2.971ArgGly: 2.971 ± 0.25
0.911ArgHis: 0.911 ± 0.167
2.827ArgIle: 2.827 ± 0.314
2.971ArgLys: 2.971 ± 0.27
3.762ArgLeu: 3.762 ± 0.308
1.677ArgMet: 1.677 ± 0.214
2.636ArgAsn: 2.636 ± 0.271
1.366ArgPro: 1.366 ± 0.185
1.605ArgGln: 1.605 ± 0.192
1.965ArgArg: 1.965 ± 0.211
2.061ArgSer: 2.061 ± 0.24
2.588ArgThr: 2.588 ± 0.234
3.594ArgVal: 3.594 ± 0.286
0.551ArgTrp: 0.551 ± 0.117
2.109ArgTyr: 2.109 ± 0.233
0.0ArgXaa: 0.0 ± 0.0
Ser
3.93SerAla: 3.93 ± 0.364
0.455SerCys: 0.455 ± 0.113
3.498SerAsp: 3.498 ± 0.28
3.498SerGlu: 3.498 ± 0.302
2.612SerPhe: 2.612 ± 0.201
4.097SerGly: 4.097 ± 0.425
0.911SerHis: 0.911 ± 0.151
3.954SerIle: 3.954 ± 0.316
4.05SerLys: 4.05 ± 0.386
4.673SerLeu: 4.673 ± 0.282
1.845SerMet: 1.845 ± 0.195
2.756SerAsn: 2.756 ± 0.209
1.653SerPro: 1.653 ± 0.251
1.725SerGln: 1.725 ± 0.224
2.851SerArg: 2.851 ± 0.255
3.451SerSer: 3.451 ± 0.374
3.738SerThr: 3.738 ± 0.334
3.57SerVal: 3.57 ± 0.242
0.695SerTrp: 0.695 ± 0.134
2.444SerTyr: 2.444 ± 0.3
0.0SerXaa: 0.0 ± 0.0
Thr
4.577ThrAla: 4.577 ± 0.5
0.383ThrCys: 0.383 ± 0.122
3.978ThrAsp: 3.978 ± 0.385
4.265ThrGlu: 4.265 ± 0.376
3.235ThrPhe: 3.235 ± 0.306
4.864ThrGly: 4.864 ± 0.436
1.15ThrHis: 1.15 ± 0.167
4.72ThrIle: 4.72 ± 0.381
4.768ThrLys: 4.768 ± 0.328
5.008ThrLeu: 5.008 ± 0.357
1.486ThrMet: 1.486 ± 0.211
3.403ThrAsn: 3.403 ± 0.387
2.492ThrPro: 2.492 ± 0.271
1.653ThrGln: 1.653 ± 0.24
2.971ThrArg: 2.971 ± 0.252
3.594ThrSer: 3.594 ± 0.318
3.762ThrThr: 3.762 ± 0.471
4.912ThrVal: 4.912 ± 0.429
0.647ThrTrp: 0.647 ± 0.114
3.019ThrTyr: 3.019 ± 0.289
0.0ThrXaa: 0.0 ± 0.0
Val
4.097ValAla: 4.097 ± 0.327
0.839ValCys: 0.839 ± 0.159
5.032ValAsp: 5.032 ± 0.416
5.367ValGlu: 5.367 ± 0.533
2.588ValPhe: 2.588 ± 0.274
4.002ValGly: 4.002 ± 0.32
1.318ValHis: 1.318 ± 0.182
4.673ValIle: 4.673 ± 0.345
5.99ValLys: 5.99 ± 0.475
5.631ValLeu: 5.631 ± 0.389
1.725ValMet: 1.725 ± 0.19
3.81ValAsn: 3.81 ± 0.335
2.204ValPro: 2.204 ± 0.278
2.348ValGln: 2.348 ± 0.231
3.139ValArg: 3.139 ± 0.279
4.121ValSer: 4.121 ± 0.382
5.32ValThr: 5.32 ± 0.388
5.032ValVal: 5.032 ± 0.434
0.503ValTrp: 0.503 ± 0.137
3.235ValTyr: 3.235 ± 0.327
0.0ValXaa: 0.0 ± 0.0
Trp
0.695TrpAla: 0.695 ± 0.116
0.048TrpCys: 0.048 ± 0.038
0.958TrpAsp: 0.958 ± 0.143
1.102TrpGlu: 1.102 ± 0.157
0.479TrpPhe: 0.479 ± 0.102
0.695TrpGly: 0.695 ± 0.125
0.264TrpHis: 0.264 ± 0.081
0.982TrpIle: 0.982 ± 0.147
0.815TrpLys: 0.815 ± 0.143
0.743TrpLeu: 0.743 ± 0.131
0.407TrpMet: 0.407 ± 0.096
0.527TrpAsn: 0.527 ± 0.11
0.0TrpPro: 0.0 ± 0.0
0.479TrpGln: 0.479 ± 0.112
0.288TrpArg: 0.288 ± 0.082
0.743TrpSer: 0.743 ± 0.136
0.839TrpThr: 0.839 ± 0.184
0.839TrpVal: 0.839 ± 0.115
0.144TrpTrp: 0.144 ± 0.062
0.743TrpTyr: 0.743 ± 0.135
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.085TyrAla: 2.085 ± 0.222
0.455TyrCys: 0.455 ± 0.108
2.851TyrAsp: 2.851 ± 0.252
3.235TyrGlu: 3.235 ± 0.283
1.629TyrPhe: 1.629 ± 0.241
2.54TyrGly: 2.54 ± 0.264
0.839TyrHis: 0.839 ± 0.136
3.403TyrIle: 3.403 ± 0.28
3.474TyrLys: 3.474 ± 0.262
3.355TyrLeu: 3.355 ± 0.293
1.414TyrMet: 1.414 ± 0.199
2.324TyrAsn: 2.324 ± 0.23
1.294TyrPro: 1.294 ± 0.176
1.414TyrGln: 1.414 ± 0.176
2.109TyrArg: 2.109 ± 0.233
2.444TyrSer: 2.444 ± 0.253
2.971TyrThr: 2.971 ± 0.327
2.947TyrVal: 2.947 ± 0.277
0.623TyrTrp: 0.623 ± 0.111
2.037TyrTyr: 2.037 ± 0.248
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 208 proteins (41734 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski