Agrobacterium phage 7-7-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Schmittlotzvirus; Agrobacterium virus 7-7-1

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 127 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J7F9G2|J7F9G2_9CAUD Putative membrane protein OS=Agrobacterium phage 7-7-1 OX=1161931 GN=7-7-1_000110 PE=4 SV=1
MM1 pKa = 6.59VTLVCAKK8 pKa = 10.24PPEE11 pKa = 4.73CPNVPVPLPPSPPAPAPIGGDD32 pKa = 2.65IVVEE36 pKa = 4.03NSDD39 pKa = 4.09TIILSGSGTTDD50 pKa = 2.79SPLRR54 pKa = 11.84AEE56 pKa = 4.24LAFPLRR62 pKa = 11.84IDD64 pKa = 4.21LASTTYY70 pKa = 11.09DD71 pKa = 3.24EE72 pKa = 4.99LLSGEE77 pKa = 4.19LQMVYY82 pKa = 9.39TAVEE86 pKa = 4.04TFSFVNAEE94 pKa = 4.46CEE96 pKa = 4.34GSVNPAIVTTLTVSVNDD113 pKa = 4.22APIGDD118 pKa = 3.82ISIASNQFSFDD129 pKa = 3.74LNEE132 pKa = 4.23AAVITPGDD140 pKa = 3.71VVKK143 pKa = 9.84ITTTEE148 pKa = 3.75ALIFDD153 pKa = 4.43YY154 pKa = 11.08LAITLVGNVTPP165 pKa = 5.0

Molecular weight: 17.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J7FAQ1|J7FAQ1_9CAUD Uncharacterized protein OS=Agrobacterium phage 7-7-1 OX=1161931 GN=7-7-1_0005 PE=4 SV=1
MM1 pKa = 7.48QINFKK6 pKa = 9.95PWGRR10 pKa = 11.84FSAFKK15 pKa = 10.22DD16 pKa = 3.1QRR18 pKa = 11.84QIRR21 pKa = 11.84RR22 pKa = 11.84FLSDD26 pKa = 2.84SADD29 pKa = 3.17IVLKK33 pKa = 10.44RR34 pKa = 11.84LKK36 pKa = 10.59DD37 pKa = 3.63GLRR40 pKa = 11.84NPPKK44 pKa = 10.31SGRR47 pKa = 11.84LYY49 pKa = 10.16FGKK52 pKa = 10.1SKK54 pKa = 9.76RR55 pKa = 11.84TIRR58 pKa = 11.84ASVNRR63 pKa = 11.84ALAEE67 pKa = 4.0YY68 pKa = 8.13PARR71 pKa = 11.84DD72 pKa = 3.25TGDD75 pKa = 3.43LFRR78 pKa = 11.84SAKK81 pKa = 9.83KK82 pKa = 10.09RR83 pKa = 11.84VGRR86 pKa = 11.84DD87 pKa = 3.12EE88 pKa = 4.55MEE90 pKa = 3.9VGTNMFYY97 pKa = 11.01SRR99 pKa = 11.84FLRR102 pKa = 11.84EE103 pKa = 4.33GTSKK107 pKa = 9.63MARR110 pKa = 11.84RR111 pKa = 11.84KK112 pKa = 9.62MSDD115 pKa = 2.87TALEE119 pKa = 4.01EE120 pKa = 4.38SLPKK124 pKa = 10.23LDD126 pKa = 3.72GKK128 pKa = 10.35IGRR131 pKa = 11.84FAYY134 pKa = 8.86WKK136 pKa = 10.51RR137 pKa = 11.84NN138 pKa = 3.35

Molecular weight: 16.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

127

0

127

21814

28

781

171.8

19.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.086 ± 0.331

1.449 ± 0.162

5.808 ± 0.21

6.455 ± 0.231

4.465 ± 0.161

7.555 ± 0.302

1.692 ± 0.125

5.813 ± 0.158

5.286 ± 0.278

7.046 ± 0.162

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.462 ± 0.113

4.548 ± 0.179

5.075 ± 0.215

3.007 ± 0.147

6.184 ± 0.247

5.996 ± 0.206

6.083 ± 0.226

7.486 ± 0.237

1.449 ± 0.095

3.058 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski