Shigella phage vB_SsoS_008

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Tunavirinae; Tunavirus; Shigella virus 008

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411AVD6|A0A411AVD6_9CAUD Tail tape measure protein OS=Shigella phage vB_SsoS_008 OX=2507846 GN=vBSsoS008_041 PE=4 SV=1
MM1 pKa = 7.19SAAFVGAYY9 pKa = 8.22QVNGVAQLVMQGVPFGMWDD28 pKa = 3.56NPNVTLEE35 pKa = 4.06SDD37 pKa = 4.54INTIWCRR44 pKa = 11.84DD45 pKa = 4.03AINKK49 pKa = 9.91QIDD52 pKa = 3.62DD53 pKa = 3.81VGAFGPVVLALITSYY68 pKa = 10.77HH69 pKa = 5.83PQGQEE74 pKa = 3.31

Molecular weight: 8.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411AVE6|A0A411AVE6_9CAUD Uncharacterized protein OS=Shigella phage vB_SsoS_008 OX=2507846 GN=vBSsoS008_057 PE=4 SV=1
MM1 pKa = 7.24KK2 pKa = 10.31RR3 pKa = 11.84IAEE6 pKa = 4.24STGTIGARR14 pKa = 11.84HH15 pKa = 5.68NNGNNGTRR23 pKa = 11.84RR24 pKa = 11.84GKK26 pKa = 10.02DD27 pKa = 2.81KK28 pKa = 10.89KK29 pKa = 10.07PRR31 pKa = 11.84QRR33 pKa = 11.84CGFYY37 pKa = 9.55IHH39 pKa = 7.18KK40 pKa = 9.92EE41 pKa = 3.74EE42 pKa = 4.11TRR44 pKa = 11.84AGLRR48 pKa = 11.84ARR50 pKa = 11.84LDD52 pKa = 3.56ALIEE56 pKa = 4.39HH57 pKa = 6.74YY58 pKa = 10.46GGPAACAKK66 pKa = 9.79ALKK69 pKa = 10.32VSNQTVQGWKK79 pKa = 9.67DD80 pKa = 3.31RR81 pKa = 11.84NMISWQGAEE90 pKa = 3.99AAHH93 pKa = 6.1RR94 pKa = 11.84AYY96 pKa = 10.34RR97 pKa = 11.84RR98 pKa = 11.84QGCKK102 pKa = 9.76GFRR105 pKa = 11.84AAWLRR110 pKa = 11.84FDD112 pKa = 5.62LKK114 pKa = 11.0FDD116 pKa = 4.08GNGKK120 pKa = 9.4CLEE123 pKa = 4.08KK124 pKa = 10.71RR125 pKa = 3.57

Molecular weight: 14.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

12127

41

670

146.1

16.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.139 ± 0.441

1.484 ± 0.142

5.822 ± 0.259

6.3 ± 0.241

4.041 ± 0.205

6.688 ± 0.306

2.045 ± 0.158

6.597 ± 0.165

6.655 ± 0.237

7.017 ± 0.261

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.373 ± 0.17

5.08 ± 0.194

3.505 ± 0.23

3.999 ± 0.201

5.632 ± 0.294

7.298 ± 0.283

5.277 ± 0.232

6.539 ± 0.3

1.303 ± 0.102

3.208 ± 0.146
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski