Streptococcus phage Javan584

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6BB43|A0A4D6BB43_9CAUD Uncharacterized protein OS=Streptococcus phage Javan584 OX=2548274 GN=Javan584_0027 PE=4 SV=1
MM1 pKa = 7.15VVPKK5 pKa = 10.18FRR7 pKa = 11.84AWDD10 pKa = 3.67TFDD13 pKa = 4.23EE14 pKa = 5.01DD15 pKa = 3.81MVNDD19 pKa = 5.29IFFSWQDD26 pKa = 3.23CGYY29 pKa = 10.67EE30 pKa = 4.11SLNEE34 pKa = 4.07CLSDD38 pKa = 3.8EE39 pKa = 4.18RR40 pKa = 11.84WKK42 pKa = 11.0FMQSTGKK49 pKa = 9.19MDD51 pKa = 2.99STGQVEE57 pKa = 5.1VYY59 pKa = 10.62AGDD62 pKa = 3.27ILYY65 pKa = 10.86YY66 pKa = 9.99PDD68 pKa = 4.1QDD70 pKa = 3.26EE71 pKa = 4.63DD72 pKa = 3.81NYY74 pKa = 11.47GIIKK78 pKa = 10.2FDD80 pKa = 4.0EE81 pKa = 4.41DD82 pKa = 3.98TLALVLDD89 pKa = 3.9NGYY92 pKa = 10.46EE93 pKa = 3.87RR94 pKa = 11.84FVYY97 pKa = 10.53GDD99 pKa = 3.38YY100 pKa = 11.76GMGKK104 pKa = 10.27VIGNIYY110 pKa = 8.63QNKK113 pKa = 10.02DD114 pKa = 3.17LVDD117 pKa = 4.03YY118 pKa = 11.04ILGGRR123 pKa = 11.84KK124 pKa = 8.83

Molecular weight: 14.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6BCK7|A0A4D6BCK7_9CAUD Uncharacterized protein OS=Streptococcus phage Javan584 OX=2548274 GN=Javan584_0006 PE=4 SV=1
MM1 pKa = 7.89RR2 pKa = 11.84LLKK5 pKa = 10.13RR6 pKa = 11.84QKK8 pKa = 10.65KK9 pKa = 9.88RR10 pKa = 11.84DD11 pKa = 3.45VDD13 pKa = 3.73HH14 pKa = 7.14LKK16 pKa = 10.89LIEE19 pKa = 4.44LATAIARR26 pKa = 11.84FFQIVAQIILEE37 pKa = 4.46IIKK40 pKa = 10.38HH41 pKa = 5.32LL42 pKa = 4.06

Molecular weight: 5.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

11128

37

1294

206.1

23.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.964 ± 0.544

0.53 ± 0.11

6.443 ± 0.278

7.89 ± 0.547

3.954 ± 0.283

6.821 ± 0.52

1.483 ± 0.16

6.767 ± 0.249

7.935 ± 0.43

7.782 ± 0.41

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.633 ± 0.198

5.652 ± 0.194

2.84 ± 0.238

3.55 ± 0.284

4.745 ± 0.251

6.156 ± 0.373

6.174 ± 0.563

6.488 ± 0.259

1.402 ± 0.146

3.792 ± 0.277
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski