Tortoise microvirus 50

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W7D3|A0A4P8W7D3_9VIRU DNA pilot protein OS=Tortoise microvirus 50 OX=2583155 PE=4 SV=1
MM1 pKa = 7.74FDD3 pKa = 3.52FSYY6 pKa = 11.25SVFLLSDD13 pKa = 3.56SVRR16 pKa = 11.84YY17 pKa = 10.01DD18 pKa = 3.11SFPVLKK24 pKa = 9.88EE25 pKa = 3.67YY26 pKa = 11.14AKK28 pKa = 9.27THH30 pKa = 5.2QPFIGFNDD38 pKa = 3.33YY39 pKa = 11.14RR40 pKa = 11.84HH41 pKa = 6.29FLTFLEE47 pKa = 4.61TTDD50 pKa = 3.63CQKK53 pKa = 11.07FILLVSKK60 pKa = 10.29FEE62 pKa = 4.28NDD64 pKa = 3.02EE65 pKa = 4.14NPAFSSFIEE74 pKa = 4.12VFDD77 pKa = 4.58PVSCTCVKK85 pKa = 10.63SFEE88 pKa = 4.25HH89 pKa = 7.07SII91 pKa = 3.56

Molecular weight: 10.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W9V4|A0A4P8W9V4_9VIRU Replication initiation protein OS=Tortoise microvirus 50 OX=2583155 PE=4 SV=1
MM1 pKa = 7.61CNSPLVAMKK10 pKa = 10.77LEE12 pKa = 4.0NVYY15 pKa = 9.55TKK17 pKa = 10.38SGKK20 pKa = 10.05NKK22 pKa = 9.29VVVLGGLAARR32 pKa = 11.84EE33 pKa = 3.93HH34 pKa = 7.17LEE36 pKa = 4.51FGDD39 pKa = 4.09SSQLNTPFLIPCGKK53 pKa = 9.97CVSCRR58 pKa = 11.84IKK60 pKa = 10.51HH61 pKa = 4.66SRR63 pKa = 11.84EE64 pKa = 3.37WAVRR68 pKa = 11.84CCHH71 pKa = 5.99EE72 pKa = 4.1ASLYY76 pKa = 10.3EE77 pKa = 4.19KK78 pKa = 10.84NCFITLTYY86 pKa = 10.81NNEE89 pKa = 4.13HH90 pKa = 6.47LPSDD94 pKa = 3.45KK95 pKa = 10.74SLHH98 pKa = 6.01LEE100 pKa = 3.77HH101 pKa = 6.58WQKK104 pKa = 10.74FMKK107 pKa = 10.37RR108 pKa = 11.84FRR110 pKa = 11.84KK111 pKa = 10.0KK112 pKa = 8.27YY113 pKa = 9.14GEE115 pKa = 4.32GIRR118 pKa = 11.84FFMCGEE124 pKa = 4.07YY125 pKa = 10.93GSVNARR131 pKa = 11.84PHH133 pKa = 4.48YY134 pKa = 9.11HH135 pKa = 6.11ACIFGFDD142 pKa = 4.08FPDD145 pKa = 4.09KK146 pKa = 10.54EE147 pKa = 3.75IWQVRR152 pKa = 11.84EE153 pKa = 3.99KK154 pKa = 10.6IPLYY158 pKa = 10.07RR159 pKa = 11.84SHH161 pKa = 7.93SLEE164 pKa = 4.0KK165 pKa = 10.09LWPFGFSTIGEE176 pKa = 4.45VTFEE180 pKa = 3.97SAAYY184 pKa = 7.43VARR187 pKa = 11.84YY188 pKa = 8.75IFKK191 pKa = 10.74KK192 pKa = 9.08ITGDD196 pKa = 3.41KK197 pKa = 10.61AAEE200 pKa = 4.31HH201 pKa = 5.94YY202 pKa = 10.51NGRR205 pKa = 11.84KK206 pKa = 9.4PEE208 pKa = 4.72FITMSRR214 pKa = 11.84RR215 pKa = 11.84PGIAHH220 pKa = 7.22DD221 pKa = 3.64WFLKK225 pKa = 9.88NEE227 pKa = 3.92RR228 pKa = 11.84DD229 pKa = 3.37IYY231 pKa = 11.62SNDD234 pKa = 3.1FLIDD238 pKa = 3.58QKK240 pKa = 10.81KK241 pKa = 10.02HH242 pKa = 6.01KK243 pKa = 9.81IRR245 pKa = 11.84PLRR248 pKa = 11.84YY249 pKa = 7.92YY250 pKa = 10.44DD251 pKa = 4.11HH252 pKa = 7.98LMEE255 pKa = 5.35IYY257 pKa = 10.68SPDD260 pKa = 3.08EE261 pKa = 3.72YY262 pKa = 11.51YY263 pKa = 10.09ILKK266 pKa = 9.69KK267 pKa = 10.33QRR269 pKa = 11.84LEE271 pKa = 3.93NLSKK275 pKa = 10.12IDD277 pKa = 3.31SFQRR281 pKa = 11.84MQDD284 pKa = 2.94KK285 pKa = 10.87EE286 pKa = 4.26KK287 pKa = 10.68FKK289 pKa = 10.78KK290 pKa = 9.98YY291 pKa = 8.07QLKK294 pKa = 10.69KK295 pKa = 10.44LVRR298 pKa = 11.84PLL300 pKa = 3.36

Molecular weight: 35.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1443

85

548

240.5

27.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.9 ± 2.204

1.317 ± 0.708

6.029 ± 1.087

5.613 ± 1.167

6.376 ± 1.478

5.128 ± 1.045

2.287 ± 0.583

5.128 ± 0.261

5.752 ± 1.955

8.593 ± 0.337

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.455 ± 0.257

5.059 ± 0.546

4.782 ± 0.79

4.019 ± 0.606

5.128 ± 0.544

9.979 ± 1.526

4.782 ± 0.599

5.267 ± 0.52

1.386 ± 0.25

4.019 ± 0.63
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski