Haloplanus vescus 
Average proteome isoelectric point is 4.9 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 2791 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|A0A1H3VKD8|A0A1H3VKD8_9EURY ABC-2 type transport system ATP-binding protein OS=Haloplanus vescus OX=555874 GN=SAMN04488065_0039 PE=4 SV=1MM1 pKa = 7.61  TDD3 pKa = 3.11  YY4 pKa = 10.67  NGKK7 pKa = 9.14  VRR9 pKa = 11.84  AVVLAALMVFSVFAGTVALSGTAAANVSSISGQSAQDD46 pKa = 3.39  LVAGQTSVTQEE57 pKa = 3.71  VTYY60 pKa = 9.95  STTIPGDD67 pKa = 3.83  TTPEE71 pKa = 4.36  TYY73 pKa = 10.3  TLDD76 pKa = 3.37  ADD78 pKa = 4.08  AYY80 pKa = 8.92  EE81 pKa = 4.81  GTSSATIQDD90 pKa = 3.4  VSVSLRR96 pKa = 11.84  NADD99 pKa = 4.14  DD100 pKa = 3.88  EE101 pKa = 5.17  LSVGTVSEE109 pKa = 4.22  SSGDD113 pKa = 3.76  YY114 pKa = 11.05  NIDD117 pKa = 3.41  IQDD120 pKa = 3.73  SGTDD124 pKa = 3.52  GTTHH128 pKa = 7.13  SFTLVLTITQDD139 pKa = 3.25  TQGASTGTYY148 pKa = 10.92  DD149 pKa = 3.82  LTLSAASGASSTVSFDD165 pKa = 3.04  ITSLSSNSDD174 pKa = 2.78  KK175 pKa = 11.01  RR176 pKa = 11.84  AGNAAGSGTFDD187 pKa = 3.21  TYY189 pKa = 11.33  DD190 pKa = 3.48  GEE192 pKa = 5.34  GYY194 pKa = 10.94  VFDD197 pKa = 4.77  GANVYY202 pKa = 10.27  QGEE205 pKa = 4.23  SDD207 pKa = 3.57  IEE209 pKa = 4.24  LGGSISGGVVKK220 pKa = 9.29  TAGNDD225 pKa = 3.16  EE226 pKa = 4.34  GVTLEE231 pKa = 4.92  VPDD234 pKa = 4.63  IPQDD238 pKa = 3.56  QATGRR243 pKa = 11.84  YY244 pKa = 5.35  TTNGSNNAPGVTVQRR259 pKa = 11.84  PRR261 pKa = 11.84  VTTLDD266 pKa = 3.27  VNNQYY271 pKa = 10.97  GG272 pKa = 3.28  
 27.77 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.705 
IPC2_protein 3.605 
IPC_protein 3.63 
Toseland    3.401 
ProMoST     3.808 
Dawson      3.643 
Bjellqvist  3.795 
Wikipedia   3.605 
Rodwell     3.452 
Grimsley    3.312 
Solomon     3.63 
Lehninger   3.579 
Nozaki      3.757 
DTASelect   4.024 
Thurlkill   3.465 
EMBOSS      3.605 
Sillero     3.757 
Patrickios  1.036 
IPC_peptide 3.617 
IPC2_peptide  3.732 
IPC2.peptide.svr19  3.718 
 Protein with the highest isoelectric point: 
>tr|A0A1H3WZD7|A0A1H3WZD7_9EURY Exonuclease III OS=Haloplanus vescus OX=555874 GN=SAMN04488065_1255 PE=4 SV=1MM1 pKa = 7.52  AAGSVALAAGLDD13 pKa = 3.68  RR14 pKa = 11.84  LVAEE18 pKa = 5.4  PPAALHH24 pKa = 5.41  PVAWLGRR31 pKa = 11.84  ALGPFDD37 pKa = 5.0  RR38 pKa = 11.84  EE39 pKa = 3.81  WRR41 pKa = 11.84  HH42 pKa = 5.6  PRR44 pKa = 11.84  LAGAAVALAVPLFVAAAAAAVVTLGARR71 pKa = 11.84  LDD73 pKa = 3.72  PRR75 pKa = 11.84  IGAVIAGLGCFAATSRR91 pKa = 11.84  RR92 pKa = 11.84  MLLDD96 pKa = 3.09  EE97 pKa = 4.52  ARR99 pKa = 11.84  AVVAASDD106 pKa = 3.89  TDD108 pKa = 4.07  LSAARR113 pKa = 11.84  TRR115 pKa = 11.84  LRR117 pKa = 11.84  ALAGRR122 pKa = 11.84  DD123 pKa = 3.59  ATDD126 pKa = 3.5  LDD128 pKa = 3.99  AGEE131 pKa = 4.28  VRR133 pKa = 11.84  SAAVEE138 pKa = 4.09  SAAEE142 pKa = 3.98  NLSDD146 pKa = 4.7  GLVAPLAGFALFAPISLPAAAGAAAWVKK174 pKa = 10.46  AVNTLDD180 pKa = 3.32  STFGYY185 pKa = 10.3  RR186 pKa = 11.84  SIPMGWAPARR196 pKa = 11.84  LDD198 pKa = 4.94  DD199 pKa = 3.97  IVMWVPARR207 pKa = 11.84  VSAALLAVVAGRR219 pKa = 11.84  PAALRR224 pKa = 11.84  EE225 pKa = 4.02  ARR227 pKa = 11.84  SFARR231 pKa = 11.84  RR232 pKa = 11.84  PASPNAGWPMATLAVVLGVRR252 pKa = 11.84  LTKK255 pKa = 10.57  HH256 pKa = 5.26  GAYY259 pKa = 10.18  DD260 pKa = 3.79  LRR262 pKa = 11.84  PSASHH267 pKa = 5.91  PTPAEE272 pKa = 3.81  AEE274 pKa = 3.93  RR275 pKa = 11.84  GVAVVSRR282 pKa = 11.84  AGWLAFGLTGVVALCC297 pKa = 4.42  
 30.3 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.328 
IPC2_protein 9.165 
IPC_protein 10.292 
Toseland    10.306 
ProMoST     10.233 
Dawson      10.438 
Bjellqvist  10.277 
Wikipedia   10.73 
Rodwell     10.306 
Grimsley    10.511 
Solomon     10.643 
Lehninger   10.599 
Nozaki      10.35 
DTASelect   10.248 
Thurlkill   10.335 
EMBOSS      10.745 
Sillero     10.394 
Patrickios  10.058 
IPC_peptide 10.643 
IPC2_peptide  9.633 
IPC2.peptide.svr19  8.513 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        2791 
0
2791 
803080
39
2172
287.7
31.26
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        11.121 ± 0.068
0.705 ± 0.015
8.884 ± 0.065
8.047 ± 0.069
3.245 ± 0.032
8.263 ± 0.052
2.021 ± 0.022
3.864 ± 0.034
1.706 ± 0.026
9.066 ± 0.052
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        1.745 ± 0.022
2.235 ± 0.023
4.669 ± 0.032
2.491 ± 0.029
6.789 ± 0.048
5.463 ± 0.041
6.61 ± 0.041
9.305 ± 0.054
1.114 ± 0.017
2.656 ± 0.027
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here