Vibrio phage LP.1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3T0IES4|A0A3T0IES4_9CAUD Uncharacterized protein OS=Vibrio phage LP.1 OX=2500150 PE=4 SV=1
MM1 pKa = 7.26ATINTYY7 pKa = 11.06DD8 pKa = 3.9LATSEE13 pKa = 5.06PIVEE17 pKa = 4.46TTLTASDD24 pKa = 4.17DD25 pKa = 3.95AVVSLNKK32 pKa = 10.37ANIALIVTNDD42 pKa = 3.22TGGDD46 pKa = 3.35LTLNIVGAGVSNAYY60 pKa = 9.55RR61 pKa = 11.84CPGVGSIDD69 pKa = 3.65LTAGAQATATDD80 pKa = 3.7AAVTKK85 pKa = 10.43INLSPEE91 pKa = 3.46ITKK94 pKa = 10.77YY95 pKa = 10.86LGDD98 pKa = 3.72GTLTITGGTGATAYY112 pKa = 9.68IIQSS116 pKa = 3.38

Molecular weight: 11.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q9R5Y2|A0A3Q9R5Y2_9CAUD Uncharacterized protein OS=Vibrio phage LP.1 OX=2500150 PE=4 SV=1
MM1 pKa = 7.76PARR4 pKa = 11.84PGKK7 pKa = 8.19TPRR10 pKa = 11.84HH11 pKa = 5.22VKK13 pKa = 9.81QEE15 pKa = 3.76YY16 pKa = 9.76AEE18 pKa = 4.2FVGRR22 pKa = 11.84MTGPRR27 pKa = 11.84TKK29 pKa = 10.66AAIAKK34 pKa = 8.51VLSIGMTGAKK44 pKa = 9.44QYY46 pKa = 11.71APLEE50 pKa = 4.33YY51 pKa = 9.0GTLMNSAFRR60 pKa = 11.84RR61 pKa = 11.84IEE63 pKa = 3.76GSRR66 pKa = 11.84MNWRR70 pKa = 11.84GIAGFTVYY78 pKa = 10.9YY79 pKa = 10.31SFYY82 pKa = 10.27LHH84 pKa = 6.65QRR86 pKa = 11.84MDD88 pKa = 3.5WNPRR92 pKa = 11.84PPDD95 pKa = 3.71KK96 pKa = 10.79KK97 pKa = 10.62AGPAWNPNARR107 pKa = 11.84PKK109 pKa = 10.59FLEE112 pKa = 4.46LGFTAPDD119 pKa = 3.94QITLMQKK126 pKa = 10.78ALGEE130 pKa = 4.33EE131 pKa = 4.3YY132 pKa = 10.54RR133 pKa = 11.84KK134 pKa = 10.36LL135 pKa = 3.55

Molecular weight: 15.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

13889

33

1158

195.6

21.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.244 ± 0.446

1.346 ± 0.183

6.696 ± 0.231

7.063 ± 0.306

3.528 ± 0.152

7.308 ± 0.275

1.793 ± 0.166

5.796 ± 0.214

5.904 ± 0.481

7.762 ± 0.276

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.945 ± 0.242

5.177 ± 0.236

3.708 ± 0.185

3.73 ± 0.201

4.723 ± 0.249

5.983 ± 0.258

6.242 ± 0.381

6.847 ± 0.223

1.526 ± 0.145

3.679 ± 0.226
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski