Gossypium barbadense (Sea-island cotton) (Egyptian cotton)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 40046 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P5XLG9|A0A2P5XLG9_GOSBA Uncharacterized protein OS=Gossypium barbadense OX=3634 GN=GOBAR_AA16507 PE=4 SV=1
MM1 pKa = 7.51IALYY5 pKa = 10.6CEE7 pKa = 4.05NRR9 pKa = 11.84SDD11 pKa = 3.51KK12 pKa = 10.65YY13 pKa = 11.56APIHH17 pKa = 6.41LFAEE21 pKa = 4.49LAGMKK26 pKa = 10.12QNEE29 pKa = 4.59DD30 pKa = 2.96FTAYY34 pKa = 10.38GEE36 pKa = 3.99EE37 pKa = 4.01HH38 pKa = 6.97RR39 pKa = 11.84AQEE42 pKa = 4.68LCMMAPISYY51 pKa = 10.13VDD53 pKa = 3.44SEE55 pKa = 4.25LTIRR59 pKa = 11.84GINIDD64 pKa = 4.25LNVTPDD70 pKa = 3.18IDD72 pKa = 4.01VVGDD76 pKa = 3.81DD77 pKa = 5.53GYY79 pKa = 11.52DD80 pKa = 3.37SSDD83 pKa = 3.43PCDD86 pKa = 3.33QKK88 pKa = 11.51VDD90 pKa = 3.71SDD92 pKa = 3.86NDD94 pKa = 3.82LDD96 pKa = 4.71VDD98 pKa = 5.98DD99 pKa = 5.83IPDD102 pKa = 5.13DD103 pKa = 4.53IDD105 pKa = 6.11DD106 pKa = 4.57EE107 pKa = 4.8DD108 pKa = 4.95VNDD111 pKa = 5.07DD112 pKa = 3.93GNINTSSIGNQIRR125 pKa = 11.84CIVIHH130 pKa = 6.3NNPGPHH136 pKa = 6.25MSFIDD141 pKa = 3.47PDD143 pKa = 3.47AAYY146 pKa = 10.42VAEE149 pKa = 4.31IPKK152 pKa = 10.45

Molecular weight: 16.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P5YPA9|A0A2P5YPA9_GOSBA F-box domain-containing protein OS=Gossypium barbadense OX=3634 GN=ES319_A01G165000v1 PE=4 SV=1
MM1 pKa = 7.08VSKK4 pKa = 10.96APAHH8 pKa = 6.14TSTKK12 pKa = 10.51VPMLLEE18 pKa = 4.57APAHH22 pKa = 6.11PRR24 pKa = 11.84HH25 pKa = 5.9QGADD29 pKa = 3.35GAQGSRR35 pKa = 11.84TTKK38 pKa = 10.01VADD41 pKa = 3.69GARR44 pKa = 11.84GSRR47 pKa = 11.84TTRR50 pKa = 11.84GLGCRR55 pKa = 11.84WCPRR59 pKa = 11.84LPHH62 pKa = 6.5DD63 pKa = 4.1QGPRR67 pKa = 11.84LPMVSEE73 pKa = 4.26APAYY77 pKa = 8.86PSHH80 pKa = 6.49QGADD84 pKa = 3.46AARR87 pKa = 11.84GSCTTNVLGVQVVRR101 pKa = 11.84PRR103 pKa = 11.84LQHH106 pKa = 4.89VQAAMGGCFGTIDD119 pKa = 3.31VLGYY123 pKa = 8.0QWCLMLRR130 pKa = 11.84RR131 pKa = 11.84DD132 pKa = 3.93QGPKK136 pKa = 9.44VPEE139 pKa = 3.75VLVPKK144 pKa = 10.84GEE146 pKa = 4.17GPGWFEE152 pKa = 4.57KK153 pKa = 10.93GGTGTTCSIIFIFQNGSPEE172 pKa = 3.76HH173 pKa = 6.25RR174 pKa = 11.84RR175 pKa = 11.84EE176 pKa = 3.89SSLDD180 pKa = 3.06HH181 pKa = 6.59HH182 pKa = 7.33RR183 pKa = 11.84NGSLATFMCLKK194 pKa = 9.87QGYY197 pKa = 9.59FMVPTLLHH205 pKa = 5.15QRR207 pKa = 11.84EE208 pKa = 4.26PKK210 pKa = 9.95ISFDD214 pKa = 3.42ARR216 pKa = 11.84PSRR219 pKa = 11.84TSDD222 pKa = 2.98APRR225 pKa = 11.84CRR227 pKa = 11.84CCPMLRR233 pKa = 11.84HH234 pKa = 6.05IKK236 pKa = 10.14GAGGARR242 pKa = 11.84WPCTTGWHH250 pKa = 5.78TLPIVYY256 pKa = 8.73EE257 pKa = 3.98ATARR261 pKa = 11.84PGAKK265 pKa = 9.81GAGGSCCKK273 pKa = 10.47RR274 pKa = 11.84GGTWMIHH281 pKa = 5.17SLLGTGHH288 pKa = 6.01ATIFEE293 pKa = 4.51SYY295 pKa = 10.33LWDD298 pKa = 3.94DD299 pKa = 3.66SFIIGDD305 pKa = 4.35RR306 pKa = 11.84FQMSEE311 pKa = 3.34IGTLFSAQRR320 pKa = 11.84SCTSEE325 pKa = 3.88APRR328 pKa = 11.84CRR330 pKa = 11.84CCSMTRR336 pKa = 11.84TMGWPPLRR344 pKa = 11.84ILPQTPKK351 pKa = 9.93IEE353 pKa = 4.23QVPEE357 pKa = 4.13PPTVEE362 pKa = 4.22SSHH365 pKa = 5.76SHH367 pKa = 5.02HH368 pKa = 7.12HH369 pKa = 6.77DD370 pKa = 2.93PRR372 pKa = 11.84YY373 pKa = 9.02ATVSRR378 pKa = 11.84GGGTANKK385 pKa = 8.31ATLPLTIPRR394 pKa = 11.84RR395 pKa = 11.84VFKK398 pKa = 11.0SSAKK402 pKa = 10.27DD403 pKa = 3.36FTHH406 pKa = 6.74RR407 pKa = 11.84SVGITLQGGTRR418 pKa = 11.84DD419 pKa = 3.52LSAAIAAPMTRR430 pKa = 11.84AIGGQRR436 pKa = 11.84PLMRR440 pKa = 11.84VGNWATGPCIASIPDD455 pKa = 3.28SDD457 pKa = 5.6LEE459 pKa = 4.21AFSYY463 pKa = 11.0NPAHH467 pKa = 6.37GRR469 pKa = 11.84ADD471 pKa = 3.39IEE473 pKa = 4.46GSKK476 pKa = 11.16SNVAMNAWLPQASYY490 pKa = 10.49PCGNFSDD497 pKa = 3.71TSSFKK502 pKa = 10.28FRR504 pKa = 11.84RR505 pKa = 11.84SKK507 pKa = 11.22GSIGHH512 pKa = 7.39AFTPNASAPTGAEE525 pKa = 3.98VVDD528 pKa = 4.27GARR531 pKa = 11.84GSRR534 pKa = 11.84TSEE537 pKa = 3.98APSKK541 pKa = 10.18ATLPLTIPRR550 pKa = 11.84RR551 pKa = 11.84VFKK554 pKa = 10.99SSAKK558 pKa = 10.07DD559 pKa = 3.14STRR562 pKa = 11.84RR563 pKa = 11.84SVGITLQGGARR574 pKa = 11.84DD575 pKa = 4.05LSAAAAAPTTRR586 pKa = 11.84ALGGQRR592 pKa = 11.84PLLRR596 pKa = 11.84VGNRR600 pKa = 11.84ATGACVASSPDD611 pKa = 3.19SDD613 pKa = 5.32LEE615 pKa = 4.35AFSHH619 pKa = 6.03NPAHH623 pKa = 7.32GSFAPLAFQPSAMTNCANQRR643 pKa = 11.84SEE645 pKa = 4.2STVRR649 pKa = 11.84RR650 pKa = 11.84PGKK653 pKa = 10.24APEE656 pKa = 4.17GVIPSPSPDD665 pKa = 3.04QHH667 pKa = 7.47AVTCSRR673 pKa = 11.84RR674 pKa = 11.84GSSSSSAPVADD685 pKa = 4.06EE686 pKa = 4.75FGTGTPVRR694 pKa = 11.84SPQSQSFSRR703 pKa = 11.84GYY705 pKa = 11.09GSIFPTSLAYY715 pKa = 9.23IVPLTRR721 pKa = 11.84GCSPWRR727 pKa = 11.84PDD729 pKa = 2.8AVMNISTATVMARR742 pKa = 11.84GRR744 pKa = 11.84CGSFSRR750 pKa = 11.84DD751 pKa = 2.64TRR753 pKa = 11.84QWLRR757 pKa = 11.84RR758 pKa = 11.84GTYY761 pKa = 8.76GGHH764 pKa = 6.1RR765 pKa = 11.84KK766 pKa = 8.93VAGAGKK772 pKa = 10.33RR773 pKa = 11.84LFRR776 pKa = 11.84PLGVQNRR783 pKa = 11.84APLRR787 pKa = 11.84CLRR790 pKa = 11.84RR791 pKa = 11.84KK792 pKa = 9.95APRR795 pKa = 11.84LRR797 pKa = 11.84VPKK800 pKa = 10.11RR801 pKa = 11.84LASSRR806 pKa = 11.84GSLTFSPLFPPQLNVLVSSSRR827 pKa = 11.84SAFQPEE833 pKa = 4.44SLGCRR838 pKa = 11.84YY839 pKa = 9.66GHH841 pKa = 7.22LPAAIDD847 pKa = 3.32SGAEE851 pKa = 3.53KK852 pKa = 10.34RR853 pKa = 11.84GPGRR857 pKa = 11.84EE858 pKa = 3.81ASRR861 pKa = 11.84SSRR864 pKa = 11.84SSNAQSGVISSHH876 pKa = 6.0ALYY879 pKa = 9.5PSHH882 pKa = 7.04AALPPSVKK890 pKa = 10.1PSGSAPAINAASRR903 pKa = 11.84TSLKK907 pKa = 10.3GSCSLTPGGAASPAGRR923 pKa = 11.84EE924 pKa = 4.2HH925 pKa = 6.86TSQLAAGCRR934 pKa = 11.84PMLLVVTIRR943 pKa = 11.84CSTGLDD949 pKa = 2.88LGLCHH954 pKa = 7.14FEE956 pKa = 3.9IHH958 pKa = 6.51HH959 pKa = 6.22SAGSCTCPLKK969 pKa = 10.13TPRR972 pKa = 11.84KK973 pKa = 7.32RR974 pKa = 11.84SRR976 pKa = 11.84RR977 pKa = 11.84IRR979 pKa = 11.84NN980 pKa = 3.17

Molecular weight: 104.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

40045

1

40046

15047206

26

6014

375.7

41.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.547 ± 0.011

1.91 ± 0.006

5.195 ± 0.008

6.437 ± 0.016

4.244 ± 0.009

6.503 ± 0.011

2.407 ± 0.005

5.413 ± 0.009

6.229 ± 0.012

9.773 ± 0.015

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.494 ± 0.005

4.634 ± 0.009

4.938 ± 0.012

3.694 ± 0.008

5.245 ± 0.01

8.8 ± 0.015

4.957 ± 0.008

6.479 ± 0.009

1.315 ± 0.004

2.774 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski