Streptococcus satellite phage Javan305

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4D5ZJ71|A0A4D5ZJ71_9VIRU Excalibur domain-containing protein OS=Streptococcus satellite phage Javan305 OX=2558625 GN=JavanS305_0008 PE=4 SV=1
MM1 pKa = 7.63TEE3 pKa = 4.01FNFDD7 pKa = 3.13IDD9 pKa = 3.82QIANNTVAKK18 pKa = 10.17VDD20 pKa = 3.4KK21 pKa = 7.97TTQIIEE27 pKa = 4.46TNTGSDD33 pKa = 2.99KK34 pKa = 11.08TLTFLEE40 pKa = 4.58KK41 pKa = 10.55LSPEE45 pKa = 3.85QQEE48 pKa = 4.62GIKK51 pKa = 10.52AHH53 pKa = 5.93VPQLVDD59 pKa = 4.1QFVTNQNALLDD70 pKa = 4.38FGQSAVEE77 pKa = 4.25GVNNTVNRR85 pKa = 11.84ILSEE89 pKa = 4.05QKK91 pKa = 10.36KK92 pKa = 9.48LQIPQVDD99 pKa = 4.03DD100 pKa = 3.72LLKK103 pKa = 9.26NTNRR107 pKa = 11.84EE108 pKa = 3.93LQGFVVKK115 pKa = 10.25YY116 pKa = 9.81KK117 pKa = 10.13NAEE120 pKa = 3.74IAEE123 pKa = 4.37LEE125 pKa = 4.24EE126 pKa = 4.85KK127 pKa = 10.82PNFLQRR133 pKa = 11.84LFNKK137 pKa = 10.1SKK139 pKa = 9.11NTLQEE144 pKa = 4.4FYY146 pKa = 10.5FDD148 pKa = 3.9SKK150 pKa = 10.03TVEE153 pKa = 3.97QKK155 pKa = 11.03LDD157 pKa = 3.19GMAAAVVKK165 pKa = 10.9QEE167 pKa = 4.6DD168 pKa = 3.74VLARR172 pKa = 11.84NIVSAEE178 pKa = 3.67MLIEE182 pKa = 5.37DD183 pKa = 3.67NTKK186 pKa = 10.67SIEE189 pKa = 4.06NLVGVISFIEE199 pKa = 4.27ASQTEE204 pKa = 4.32AGNRR208 pKa = 11.84ATEE211 pKa = 4.45LKK213 pKa = 10.61AQADD217 pKa = 3.86QLDD220 pKa = 4.2TSTVEE225 pKa = 4.17YY226 pKa = 8.76QTKK229 pKa = 9.32SQEE232 pKa = 4.07LARR235 pKa = 11.84MAEE238 pKa = 4.18VVNTLEE244 pKa = 4.2QQHH247 pKa = 5.54TEE249 pKa = 4.22YY250 pKa = 11.1VSRR253 pKa = 11.84LYY255 pKa = 10.39VAWATTPQMRR265 pKa = 11.84NLVKK269 pKa = 10.62VSSDD273 pKa = 2.97MRR275 pKa = 11.84QKK277 pKa = 11.16LGMLRR282 pKa = 11.84RR283 pKa = 11.84NTIPTMKK290 pKa = 10.54LSIAQLGILQQSMKK304 pKa = 10.45SGVVADD310 pKa = 5.39AIVNANNAALQMLAEE325 pKa = 4.34TSKK328 pKa = 11.04EE329 pKa = 4.07VIPQMEE335 pKa = 5.32RR336 pKa = 11.84IAQSPTVAVEE346 pKa = 4.27SVTQLAEE353 pKa = 3.99SLVAQNQGIVAAIEE367 pKa = 3.82LGRR370 pKa = 11.84QKK372 pKa = 10.42RR373 pKa = 11.84AQLEE377 pKa = 4.28TTIVKK382 pKa = 9.44SAEE385 pKa = 4.12MINDD389 pKa = 3.62SVKK392 pKa = 10.67LRR394 pKa = 11.84DD395 pKa = 3.71EE396 pKa = 5.1KK397 pKa = 10.74IVQALLDD404 pKa = 3.69QGKK407 pKa = 8.96AAQKK411 pKa = 9.8EE412 pKa = 4.34VQEE415 pKa = 4.29

Molecular weight:
46.21 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4D5ZJ41|A0A4D5ZJ41_9VIRU Putative toxic anion resistance protein OS=Streptococcus satellite phage Javan305 OX=2558625 GN=JavanS305_0011 PE=4 SV=1
MM1 pKa = 7.65KK2 pKa = 9.94IAEE5 pKa = 4.22IKK7 pKa = 9.09KK8 pKa = 9.16QNGSTVYY15 pKa = 10.39RR16 pKa = 11.84SRR18 pKa = 11.84VYY20 pKa = 10.84LGIDD24 pKa = 2.94MTTGKK29 pKa = 10.37RR30 pKa = 11.84IQANLTARR38 pKa = 11.84TKK40 pKa = 10.67TEE42 pKa = 4.16LKK44 pKa = 10.56KK45 pKa = 10.53KK46 pKa = 10.04AQLAKK51 pKa = 10.9AEE53 pKa = 4.16FQNNGCTKK61 pKa = 10.48KK62 pKa = 9.28EE63 pKa = 4.32TISLTSYY70 pKa = 11.24EE71 pKa = 4.31EE72 pKa = 4.38VAQLWWEE79 pKa = 4.57SYY81 pKa = 7.5QHH83 pKa = 5.01TVKK86 pKa = 10.72PNTQDD91 pKa = 3.01SVKK94 pKa = 10.42RR95 pKa = 11.84LLANHH100 pKa = 6.83VIPLFGSYY108 pKa = 10.42RR109 pKa = 11.84LDD111 pKa = 3.34KK112 pKa = 10.39LTTPTIQRR120 pKa = 11.84IVNQLALRR128 pKa = 11.84ANKK131 pKa = 10.05RR132 pKa = 11.84EE133 pKa = 3.95EE134 pKa = 4.26GAFLHH139 pKa = 6.3YY140 pKa = 10.87DD141 pKa = 4.12KK142 pKa = 11.05IHH144 pKa = 6.59ALNKK148 pKa = 10.22RR149 pKa = 11.84ILQYY153 pKa = 11.19AVTMQIIDD161 pKa = 3.61INPARR166 pKa = 11.84EE167 pKa = 3.9VILPRR172 pKa = 11.84KK173 pKa = 8.41IKK175 pKa = 10.35KK176 pKa = 9.35GRR178 pKa = 11.84NKK180 pKa = 9.77VKK182 pKa = 10.46HH183 pKa = 5.64FNNLEE188 pKa = 3.99LKK190 pKa = 10.51QFLSYY195 pKa = 11.05LEE197 pKa = 4.12EE198 pKa = 4.98ANLAIYY204 pKa = 10.3RR205 pKa = 11.84NIYY208 pKa = 10.05EE209 pKa = 3.68ITLYY213 pKa = 10.92KK214 pKa = 10.38FLLATGCRR222 pKa = 11.84INEE225 pKa = 3.92ALALHH230 pKa = 6.77WSDD233 pKa = 4.46IDD235 pKa = 3.63LHH237 pKa = 6.01NATVNITKK245 pKa = 9.15TLNHH249 pKa = 6.65LGEE252 pKa = 4.45INSPKK257 pKa = 10.27SEE259 pKa = 3.73ASYY262 pKa = 8.95RR263 pKa = 11.84TIDD266 pKa = 4.0IDD268 pKa = 3.64PQTIEE273 pKa = 3.99VLKK276 pKa = 10.61VYY278 pKa = 9.35QKK280 pKa = 10.51RR281 pKa = 11.84QRR283 pKa = 11.84QEE285 pKa = 2.93AWKK288 pKa = 10.19LGRR291 pKa = 11.84TEE293 pKa = 4.07TVVFSDD299 pKa = 6.26FIHH302 pKa = 7.25KK303 pKa = 10.29YY304 pKa = 9.53PNNKK308 pKa = 8.18TLSTRR313 pKa = 11.84LNTRR317 pKa = 11.84FKK319 pKa = 10.85HH320 pKa = 6.0AGVPNIGFHH329 pKa = 6.22GFRR332 pKa = 11.84HH333 pKa = 4.87THH335 pKa = 6.58ASLLLNSGIPYY346 pKa = 10.24KK347 pKa = 10.53KK348 pKa = 9.14LQHH351 pKa = 6.53RR352 pKa = 11.84LGHH355 pKa = 5.57STLAMTMDD363 pKa = 4.54TYY365 pKa = 11.85SHH367 pKa = 7.08LSKK370 pKa = 10.76EE371 pKa = 4.21KK372 pKa = 10.8AKK374 pKa = 9.65TAVSFYY380 pKa = 9.94EE381 pKa = 4.13KK382 pKa = 10.49AVSSLL387 pKa = 3.39

Molecular weight:
44.63 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

0

13

3397

56

513

261.3

29.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.035 ± 0.601

0.648 ± 0.179

5.328 ± 0.527

8.331 ± 0.738

3.209 ± 0.387

4.268 ± 0.302

1.913 ± 0.42

8.36 ± 0.622

9.361 ± 0.667

8.979 ± 0.734

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.855 ± 0.23

5.475 ± 0.39

2.679 ± 0.272

4.475 ± 0.863

3.945 ± 0.432

8.184 ± 0.779

6.094 ± 0.917

5.946 ± 0.545

0.883 ± 0.182

4.033 ± 0.531

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski