Pseudoroseicyclus sp. CLL3-39

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Pseudoroseicyclus

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3556 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B2JUS4|A0A6B2JUS4_9RHOB Uncharacterized protein OS=Pseudoroseicyclus sp. CLL3-39 OX=2705421 GN=GZA08_02975 PE=4 SV=1
MM1 pKa = 8.12DD2 pKa = 6.09DD3 pKa = 3.82EE4 pKa = 5.07AADD7 pKa = 3.9TATDD11 pKa = 3.55EE12 pKa = 5.2APTLDD17 pKa = 3.56EE18 pKa = 4.51VEE20 pKa = 4.63PGEE23 pKa = 4.8DD24 pKa = 3.18ALFVDD29 pKa = 5.21DD30 pKa = 6.48LEE32 pKa = 4.63TATDD36 pKa = 3.96DD37 pKa = 4.0EE38 pKa = 5.19PGAEE42 pKa = 3.8ATASEE47 pKa = 4.81EE48 pKa = 4.07EE49 pKa = 4.4SLDD52 pKa = 3.87AVIDD56 pKa = 3.92PAPEE60 pKa = 3.85TGEE63 pKa = 3.96TSEE66 pKa = 4.74APEE69 pKa = 5.02DD70 pKa = 4.09GPAATEE76 pKa = 3.94PEE78 pKa = 4.21PVFEE82 pKa = 5.83VGADD86 pKa = 3.11WPYY89 pKa = 10.58WGGDD93 pKa = 3.16ADD95 pKa = 3.84ATRR98 pKa = 11.84YY99 pKa = 10.33SPLAQITPQNVEE111 pKa = 3.83EE112 pKa = 4.0LVEE115 pKa = 4.2VFSYY119 pKa = 8.99QTGDD123 pKa = 3.05MPTEE127 pKa = 4.26VTEE130 pKa = 4.34GTYY133 pKa = 10.58SPEE136 pKa = 3.91TTPLKK141 pKa = 10.95LGDD144 pKa = 4.42DD145 pKa = 4.46LLMCSAMNILISIDD159 pKa = 3.28AATGEE164 pKa = 4.52EE165 pKa = 3.76NWRR168 pKa = 11.84FNPGVPPEE176 pKa = 4.77AIPYY180 pKa = 8.85GATCRR185 pKa = 11.84GVSVYY190 pKa = 10.4RR191 pKa = 11.84DD192 pKa = 3.42PDD194 pKa = 3.45AGEE197 pKa = 4.84GDD199 pKa = 3.72LCATRR204 pKa = 11.84VIQATLDD211 pKa = 3.56AKK213 pKa = 10.71LVAVDD218 pKa = 4.75AEE220 pKa = 4.51TGLPCEE226 pKa = 4.74DD227 pKa = 3.91FGEE230 pKa = 4.41NGTVDD235 pKa = 3.53LWQDD239 pKa = 2.44IGEE242 pKa = 4.63RR243 pKa = 11.84VPGWYY248 pKa = 10.17AVTAPPAIVRR258 pKa = 11.84GVVVTGAQVLDD269 pKa = 4.32GQAEE273 pKa = 4.08EE274 pKa = 4.56APSGVVRR281 pKa = 11.84GYY283 pKa = 10.95DD284 pKa = 3.16ALTGEE289 pKa = 5.21LAWAWDD295 pKa = 3.58LAAPEE300 pKa = 4.33EE301 pKa = 4.49MRR303 pKa = 11.84DD304 pKa = 3.74GPPEE308 pKa = 3.82GRR310 pKa = 11.84VYY312 pKa = 10.87TRR314 pKa = 11.84GTPNMWTTAVADD326 pKa = 3.84EE327 pKa = 4.26EE328 pKa = 4.35LGYY331 pKa = 11.16VYY333 pKa = 10.77LPLGNSSVDD342 pKa = 3.48YY343 pKa = 10.41YY344 pKa = 11.56GSNRR348 pKa = 11.84SEE350 pKa = 4.17AEE352 pKa = 4.06NEE354 pKa = 3.92FSTSLVAVDD363 pKa = 3.53ATTGEE368 pKa = 4.68EE369 pKa = 3.94VWHH372 pKa = 5.93FQTVRR377 pKa = 11.84RR378 pKa = 11.84DD379 pKa = 2.9VWDD382 pKa = 3.73YY383 pKa = 11.96DD384 pKa = 4.13LGSQPTLVDD393 pKa = 3.36WQGTPAIILPSKK405 pKa = 10.61QGDD408 pKa = 3.49IYY410 pKa = 10.89ILDD413 pKa = 4.02RR414 pKa = 11.84ATGEE418 pKa = 3.95PLTPVGQLEE427 pKa = 4.65DD428 pKa = 3.84LPQGEE433 pKa = 4.95IEE435 pKa = 4.45PDD437 pKa = 3.99YY438 pKa = 11.29ISDD441 pKa = 3.74TQPYY445 pKa = 9.0SVWHH449 pKa = 5.75TLRR452 pKa = 11.84KK453 pKa = 9.3PPLEE457 pKa = 5.22AKK459 pKa = 9.83DD460 pKa = 3.38AWGFTPIDD468 pKa = 3.74QLWCRR473 pKa = 11.84IQFHH477 pKa = 6.18RR478 pKa = 11.84ANYY481 pKa = 9.07EE482 pKa = 4.15GYY484 pKa = 7.72FTPPSADD491 pKa = 3.67APWIQYY497 pKa = 9.29PGYY500 pKa = 10.62NGGSDD505 pKa = 3.32WGSVAVDD512 pKa = 3.25VEE514 pKa = 4.25RR515 pKa = 11.84GIIIANYY522 pKa = 10.31NDD524 pKa = 3.23IPNYY528 pKa = 8.76NQLIEE533 pKa = 5.54RR534 pKa = 11.84EE535 pKa = 4.4DD536 pKa = 3.98PYY538 pKa = 10.92QTDD541 pKa = 3.79EE542 pKa = 4.17YY543 pKa = 9.11TTIDD547 pKa = 3.85EE548 pKa = 4.62APEE551 pKa = 4.45SVGSPADD558 pKa = 3.67NEE560 pKa = 4.46AGPQAGSPYY569 pKa = 10.06AISVNAGWRR578 pKa = 11.84SEE580 pKa = 4.06LTGMPCSSPPYY591 pKa = 9.69GGIRR595 pKa = 11.84AIDD598 pKa = 3.95LEE600 pKa = 4.43TGEE603 pKa = 4.58TLWDD607 pKa = 3.9RR608 pKa = 11.84PLGTARR614 pKa = 11.84RR615 pKa = 11.84NGPFGIPSFLPLDD628 pKa = 3.37IGTPNNGGSVVTASGLVFIGAATDD652 pKa = 3.48NLFRR656 pKa = 11.84AIDD659 pKa = 3.58IEE661 pKa = 4.32TGEE664 pKa = 4.45TLWSVVLPAGGQANPVTYY682 pKa = 10.12EE683 pKa = 3.57VDD685 pKa = 2.8GRR687 pKa = 11.84QYY689 pKa = 12.02VMISAMGHH697 pKa = 5.83HH698 pKa = 6.34FMEE701 pKa = 5.06TGVGDD706 pKa = 4.76YY707 pKa = 10.49MMAWALPEE715 pKa = 3.81

Molecular weight: 77.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B2JLW5|A0A6B2JLW5_9RHOB Response regulator transcription factor OS=Pseudoroseicyclus sp. CLL3-39 OX=2705421 GN=GZA08_16455 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84VRR12 pKa = 11.84KK13 pKa = 9.2ARR15 pKa = 11.84HH16 pKa = 4.71GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.97VLSAA44 pKa = 4.11

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3556

0

3556

1122436

21

2097

315.6

34.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.483 ± 0.065

0.786 ± 0.013

5.482 ± 0.038

6.893 ± 0.039

3.517 ± 0.024

9.302 ± 0.046

1.994 ± 0.019

4.709 ± 0.031

2.319 ± 0.034

10.475 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.628 ± 0.02

2.094 ± 0.02

5.659 ± 0.036

2.818 ± 0.023

7.157 ± 0.047

4.925 ± 0.026

5.174 ± 0.026

6.992 ± 0.036

1.471 ± 0.02

2.123 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski