Flavobacterium phage Fpv6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B0WLD3|A0A1B0WLD3_9CAUD Uncharacterized protein OS=Flavobacterium phage Fpv6 OX=1814286 PE=4 SV=1
MM1 pKa = 7.12VKK3 pKa = 10.44VIEE6 pKa = 4.15RR7 pKa = 11.84TYY9 pKa = 11.5NEE11 pKa = 3.89IFTPEE16 pKa = 3.98STSWLLGNVGEE27 pKa = 4.19WQKK30 pKa = 11.54LKK32 pKa = 11.1LKK34 pKa = 10.9VEE36 pKa = 4.22VSVDD40 pKa = 3.87LYY42 pKa = 11.17CDD44 pKa = 3.07QEE46 pKa = 4.01NRR48 pKa = 11.84LKK50 pKa = 10.18IDD52 pKa = 3.65YY53 pKa = 10.12INNSFVLNDD62 pKa = 3.51GKK64 pKa = 10.87KK65 pKa = 8.31WSDD68 pKa = 3.23YY69 pKa = 11.43GFDD72 pKa = 3.18IGQNIQLVYY81 pKa = 10.64KK82 pKa = 10.43FKK84 pKa = 11.14LDD86 pKa = 3.63TNGDD90 pKa = 4.01GIYY93 pKa = 10.22EE94 pKa = 4.37GPFYY98 pKa = 10.2QAPNFTIVNIASNVMEE114 pKa = 4.24VLQPIVIWVLADD126 pKa = 4.1TIPYY130 pKa = 8.93NQDD133 pKa = 2.34SVRR136 pKa = 11.84IIDD139 pKa = 3.77CKK141 pKa = 10.69FIAFVEE147 pKa = 4.36PEE149 pKa = 4.07GCRR152 pKa = 11.84IVYY155 pKa = 8.92GQISNEE161 pKa = 3.71NSEE164 pKa = 4.3NLSLQSFIDD173 pKa = 3.62GTTTMFVQPNVKK185 pKa = 9.93SQSLNINSSMNAVGMQSGMSVRR207 pKa = 11.84MVSVKK212 pKa = 10.44RR213 pKa = 11.84IGLKK217 pKa = 10.16PGSQNVYY224 pKa = 9.29QYY226 pKa = 11.08EE227 pKa = 4.12FEE229 pKa = 4.18LQYY232 pKa = 10.96LISSFFEE239 pKa = 4.43NISNFTNNQMPDD251 pKa = 3.37YY252 pKa = 10.39LLGDD256 pKa = 4.07GSLTDD261 pKa = 3.45NFQILFYY268 pKa = 10.36PKK270 pKa = 9.29WNNPNTFIQNDD281 pKa = 3.62MKK283 pKa = 10.43KK284 pKa = 8.0TARR287 pKa = 11.84LGNTGWFDD295 pKa = 3.56EE296 pKa = 4.86NYY298 pKa = 10.94NEE300 pKa = 4.82LEE302 pKa = 4.08NNFVIEE308 pKa = 4.06SLQYY312 pKa = 10.81FDD314 pKa = 6.38DD315 pKa = 4.01NGNPVDD321 pKa = 5.06AIDD324 pKa = 3.74YY325 pKa = 7.49FAPTKK330 pKa = 10.82VKK332 pKa = 10.74GIVSGVQNINTNTEE346 pKa = 3.97CGFGFIWVPKK356 pKa = 10.4FEE358 pKa = 4.5EE359 pKa = 4.73DD360 pKa = 3.91YY361 pKa = 11.21KK362 pKa = 11.42NKK364 pKa = 7.82EE365 pKa = 3.98TPFYY369 pKa = 11.18QNTFVNTGTTTDD381 pKa = 3.39GFKK384 pKa = 10.91VGTFYY389 pKa = 11.07PLTNVGGGLNSASIDD404 pKa = 3.57TKK406 pKa = 10.55NVKK409 pKa = 8.64FTDD412 pKa = 3.03IGGGKK417 pKa = 9.35ISFEE421 pKa = 4.52MIFTPNPNFYY431 pKa = 10.93LFFDD435 pKa = 4.38ARR437 pKa = 11.84NDD439 pKa = 3.71DD440 pKa = 3.53DD441 pKa = 4.51RR442 pKa = 11.84KK443 pKa = 9.43YY444 pKa = 11.16VLWLSVADD452 pKa = 3.6QTLVRR457 pKa = 11.84NFSDD461 pKa = 3.49RR462 pKa = 11.84VSLLVDD468 pKa = 4.62LNSMIKK474 pKa = 8.78TVPPAGPYY482 pKa = 10.02PYY484 pKa = 9.67IQNAFLEE491 pKa = 4.7HH492 pKa = 6.94PFDD495 pKa = 4.22EE496 pKa = 4.59NAIGVDD502 pKa = 3.67EE503 pKa = 4.51YY504 pKa = 11.58DD505 pKa = 4.52GFVQDD510 pKa = 6.08DD511 pKa = 4.15VLCRR515 pKa = 11.84LPFQLDD521 pKa = 4.15PITTEE526 pKa = 3.9FKK528 pKa = 10.77KK529 pKa = 9.99MRR531 pKa = 11.84FAIEE535 pKa = 4.05IYY537 pKa = 10.59NPSTGVKK544 pKa = 10.02KK545 pKa = 10.4ILEE548 pKa = 4.33KK549 pKa = 10.65FDD551 pKa = 4.09VDD553 pKa = 3.37MTQFFDD559 pKa = 4.16DD560 pKa = 4.43ANGVPQFNLNQTRR573 pKa = 11.84GFKK576 pKa = 10.74LEE578 pKa = 4.08NGNNKK583 pKa = 8.9NWLKK587 pKa = 10.68INRR590 pKa = 11.84EE591 pKa = 3.78PSLDD595 pKa = 3.32SGGRR599 pKa = 11.84FGYY602 pKa = 10.45LAFYY606 pKa = 10.2AFKK609 pKa = 10.33IRR611 pKa = 11.84YY612 pKa = 7.6EE613 pKa = 3.89DD614 pKa = 3.82WINFPGMPNDD624 pKa = 4.01FFSSTEE630 pKa = 3.94LNNGFHH636 pKa = 7.07NDD638 pKa = 3.17WIHH641 pKa = 5.74YY642 pKa = 8.4FNTIGWQMNFYY653 pKa = 10.21TEE655 pKa = 3.67IDD657 pKa = 3.18AVEE660 pKa = 4.01NNEE663 pKa = 4.06LKK665 pKa = 10.43RR666 pKa = 11.84YY667 pKa = 8.9KK668 pKa = 10.77NKK670 pKa = 10.28FKK672 pKa = 11.0FKK674 pKa = 10.83FKK676 pKa = 10.74DD677 pKa = 3.2YY678 pKa = 11.24DD679 pKa = 3.98LNQLISTQHH688 pKa = 6.02RR689 pKa = 11.84YY690 pKa = 9.62YY691 pKa = 10.59RR692 pKa = 11.84DD693 pKa = 3.23SDD695 pKa = 3.67NTLLNVGTDD704 pKa = 4.42PISGRR709 pKa = 11.84PLGVIISNEE718 pKa = 3.8PTRR721 pKa = 11.84LEE723 pKa = 3.71IEE725 pKa = 4.23YY726 pKa = 10.26EE727 pKa = 4.06IMDD730 pKa = 4.12GGTWDD735 pKa = 3.24IANVYY740 pKa = 8.23ATATIEE746 pKa = 3.86IDD748 pKa = 3.01KK749 pKa = 10.85GAGIMQMRR757 pKa = 11.84QLSSVWGSEE766 pKa = 3.48NDD768 pKa = 3.41NPLIPLPGQTKK779 pKa = 10.54LKK781 pKa = 10.36IEE783 pKa = 4.0VDD785 pKa = 3.32ATLKK789 pKa = 10.9RR790 pKa = 11.84LTTKK794 pKa = 10.69CLIDD798 pKa = 5.02PDD800 pKa = 4.58LLDD803 pKa = 3.73DD804 pKa = 5.42AIRR807 pKa = 11.84YY808 pKa = 8.21RR809 pKa = 11.84ITGRR813 pKa = 11.84VGCFANGGSFEE824 pKa = 4.1FGKK827 pKa = 11.04YY828 pKa = 6.16EE829 pKa = 4.06ARR831 pKa = 11.84YY832 pKa = 7.89EE833 pKa = 4.16DD834 pKa = 4.57KK835 pKa = 11.59YY836 pKa = 11.13EE837 pKa = 3.81

Molecular weight: 96.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B0WLH7|A0A1B0WLH7_9CAUD Uncharacterized protein OS=Flavobacterium phage Fpv6 OX=1814286 PE=4 SV=1
MM1 pKa = 7.58EE2 pKa = 5.63NYY4 pKa = 10.18NPSSQAKK11 pKa = 8.79LIRR14 pKa = 11.84MIFNKK19 pKa = 8.19NHH21 pKa = 6.76KK22 pKa = 9.85IILSDD27 pKa = 3.78EE28 pKa = 3.96QLKK31 pKa = 10.01TIDD34 pKa = 3.54IAFWDD39 pKa = 3.87YY40 pKa = 11.4KK41 pKa = 10.72KK42 pKa = 11.08EE43 pKa = 3.78KK44 pKa = 10.26LYY46 pKa = 10.89DD47 pKa = 2.99IGIYY51 pKa = 10.03RR52 pKa = 11.84GINICLFFILSLISFFTTTSKK73 pKa = 9.62WLKK76 pKa = 9.11IVLIIFFFYY85 pKa = 10.96NIIHH89 pKa = 7.09LLLTIKK95 pKa = 10.37RR96 pKa = 11.84YY97 pKa = 9.46KK98 pKa = 9.05QLKK101 pKa = 9.9NSFEE105 pKa = 4.09

Molecular weight: 12.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

13535

71

1103

233.4

26.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.625 ± 0.285

1.212 ± 0.162

5.807 ± 0.207

7.824 ± 0.417

5.696 ± 0.283

4.898 ± 0.325

1.322 ± 0.135

8.615 ± 0.272

9.945 ± 0.544

8.053 ± 0.309

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.268 ± 0.148

7.536 ± 0.363

2.985 ± 0.236

4.463 ± 0.385

3.258 ± 0.218

5.445 ± 0.228

5.911 ± 0.25

5.297 ± 0.253

1.093 ± 0.114

3.746 ± 0.214
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski