Diaminobutyricimonas aerilata

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Diaminobutyricimonas

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3263 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2M9CN69|A0A2M9CN69_9MICO Putative dehydrogenase OS=Diaminobutyricimonas aerilata OX=1162967 GN=CLV46_2943 PE=4 SV=1
MM1 pKa = 7.46GLAIATVIATAALTLAGGLPAQAVGPAEE29 pKa = 4.29LSIEE33 pKa = 4.02ITPVNPTTGEE43 pKa = 4.32VITEE47 pKa = 4.1TAFNQHH53 pKa = 5.87NDD55 pKa = 3.0RR56 pKa = 11.84LAYY59 pKa = 10.11RR60 pKa = 11.84IGYY63 pKa = 7.74SCSVAEE69 pKa = 4.38CTDD72 pKa = 3.43AVVTLPAVATDD83 pKa = 3.41PTYY86 pKa = 10.86GQFRR90 pKa = 11.84LHH92 pKa = 7.19HH93 pKa = 6.25YY94 pKa = 7.33EE95 pKa = 3.65TWTPPAGGGATITNSEE111 pKa = 4.22TAGITVNLGTLPAGTSATFQVQYY134 pKa = 10.82IRR136 pKa = 11.84YY137 pKa = 7.45TGGSAPNPLEE147 pKa = 3.92AAYY150 pKa = 9.35FPPGYY155 pKa = 9.92QIEE158 pKa = 4.27RR159 pKa = 11.84SATISSPNATADD171 pKa = 3.37ATAVASPVTWNIQIPTPAIGKK192 pKa = 9.56VGLTATTRR200 pKa = 11.84PDD202 pKa = 2.63TDD204 pKa = 3.33YY205 pKa = 10.45TYY207 pKa = 10.67RR208 pKa = 11.84IAMSDD213 pKa = 3.44GCFVNQGAARR223 pKa = 11.84WLATGPYY230 pKa = 9.65LCAEE234 pKa = 4.35NFTVVDD240 pKa = 4.0TLPDD244 pKa = 3.23QAEE247 pKa = 4.32FVSATGGGVYY257 pKa = 10.3DD258 pKa = 4.69PEE260 pKa = 4.5THH262 pKa = 5.44TVTWTGSGPRR272 pKa = 11.84AAGGWGVALYY282 pKa = 9.14TGWSTGNGYY291 pKa = 11.07ANRR294 pKa = 11.84FVTVRR299 pKa = 11.84FPADD303 pKa = 3.7AFPEE307 pKa = 4.37AAGGADD313 pKa = 4.79FIAPVTNDD321 pKa = 2.53IEE323 pKa = 4.4ATVTYY328 pKa = 10.53LDD330 pKa = 4.59DD331 pKa = 4.23AQTTKK336 pKa = 10.36TVTASSEE343 pKa = 4.13NQVIRR348 pKa = 11.84AAPFGRR354 pKa = 11.84ANQTKK359 pKa = 8.63DD360 pKa = 3.11TTSDD364 pKa = 3.41QIIGGQRR371 pKa = 11.84FVNVPPDD378 pKa = 3.65TTGLVCPEE386 pKa = 4.59SGRR389 pKa = 11.84DD390 pKa = 3.46DD391 pKa = 3.36WGTTCTPGGAVATYY405 pKa = 7.53PTQLEE410 pKa = 4.34YY411 pKa = 10.44WQVDD415 pKa = 3.66TYY417 pKa = 11.91NRR419 pKa = 11.84GNVPGVAVITDD430 pKa = 3.83DD431 pKa = 5.39DD432 pKa = 5.1LGDD435 pKa = 3.68SALRR439 pKa = 11.84VYY441 pKa = 10.42QINTSAAATIAVTLSDD457 pKa = 3.95GTPARR462 pKa = 11.84ATGTQYY468 pKa = 10.97VAPAGLRR475 pKa = 11.84IIAATVTSGPIAGPNLLPSQNAGTLFRR502 pKa = 11.84TLYY505 pKa = 10.04RR506 pKa = 11.84YY507 pKa = 7.34EE508 pKa = 4.29VPVGAPTDD516 pKa = 3.88VAWTNTATATMSYY529 pKa = 10.52PEE531 pKa = 4.26YY532 pKa = 10.41PEE534 pKa = 4.42IADD537 pKa = 3.43IPMEE541 pKa = 4.3ATATANFRR549 pKa = 11.84DD550 pKa = 4.3WPTVTVPVTPPTFGAGFASAPVVEE574 pKa = 5.19GGGQVVPGGRR584 pKa = 11.84VTFGVRR590 pKa = 11.84GGTANIPADD599 pKa = 3.95RR600 pKa = 11.84DD601 pKa = 3.5VSPQYY606 pKa = 11.64VFIAPVGWAVVPNSASFPAGSVPPGVAYY634 pKa = 9.12TYY636 pKa = 11.17KK637 pKa = 10.5DD638 pKa = 3.3VTIAGVTRR646 pKa = 11.84QAVIASWPTGTTFGKK661 pKa = 10.18NVTWPTMNVVASPTSAVAAGTNSVATAWAGDD692 pKa = 3.48SRR694 pKa = 11.84NAYY697 pKa = 9.85EE698 pKa = 5.39PDD700 pKa = 3.21TTTWNVKK707 pKa = 9.79VVDD710 pKa = 3.98TTDD713 pKa = 2.77VDD715 pKa = 3.7GDD717 pKa = 4.23GNSTEE722 pKa = 4.86AFAAGNSAAVLVSGTSRR739 pKa = 11.84LDD741 pKa = 3.45TVKK744 pKa = 10.35EE745 pKa = 3.84ICVLDD750 pKa = 3.78EE751 pKa = 5.55DD752 pKa = 5.64GACEE756 pKa = 4.05WVSNPDD762 pKa = 2.59IVAGVDD768 pKa = 3.41PDD770 pKa = 3.78ATDD773 pKa = 2.76ITYY776 pKa = 10.48RR777 pKa = 11.84ITFSNGSNQTLSNVVGYY794 pKa = 10.5DD795 pKa = 3.18VLPYY799 pKa = 10.76VGDD802 pKa = 3.7PRR804 pKa = 11.84GSTFGEE810 pKa = 4.24TLNEE814 pKa = 4.19VTSVSPNLEE823 pKa = 3.85LSYY826 pKa = 10.9SASTNPCRR834 pKa = 11.84PEE836 pKa = 3.89VLSTNPGCEE845 pKa = 4.15TGWTGSADD853 pKa = 3.43GARR856 pKa = 11.84SIRR859 pKa = 11.84AALTGTLAPGASASFTFTASVVPGAAADD887 pKa = 3.81AVACNSVASDD897 pKa = 3.65SASTLPAEE905 pKa = 4.52PRR907 pKa = 11.84PVCATTQEE915 pKa = 3.78ADD917 pKa = 3.67LAVTVPSRR925 pKa = 11.84LPLQAEE931 pKa = 4.29RR932 pKa = 11.84PGVVPFTVANLGPSANAPATVEE954 pKa = 3.69IDD956 pKa = 3.46VPAGIRR962 pKa = 11.84ITSLDD967 pKa = 3.49PDD969 pKa = 3.86GWVCAADD976 pKa = 3.73EE977 pKa = 4.37VEE979 pKa = 4.49PDD981 pKa = 3.62GSVLGPVTLSCEE993 pKa = 3.96PVTAEE998 pKa = 3.59GTARR1002 pKa = 11.84MLAIDD1007 pKa = 4.67EE1008 pKa = 4.73PNALDD1013 pKa = 4.14LPAVIPSDD1021 pKa = 3.56EE1022 pKa = 4.18LVGEE1026 pKa = 4.76GACFAAAVSGLMSDD1040 pKa = 3.63PVEE1043 pKa = 4.45EE1044 pKa = 4.33NDD1046 pKa = 3.55TAEE1049 pKa = 3.92ACFDD1053 pKa = 3.84VVQGDD1058 pKa = 4.16TLVGLTKK1065 pKa = 10.59DD1066 pKa = 3.79DD1067 pKa = 4.03GLGSVAMGEE1076 pKa = 4.24EE1077 pKa = 4.1FTYY1080 pKa = 10.44TIDD1083 pKa = 3.79VANLLVGEE1091 pKa = 4.36GLSDD1095 pKa = 3.88IVITDD1100 pKa = 3.83EE1101 pKa = 5.43LPPTLAFVSASAGGSIEE1118 pKa = 4.12GQGDD1122 pKa = 3.5ADD1124 pKa = 4.35ADD1126 pKa = 3.83GLRR1129 pKa = 11.84AGGTVTWNLASLAASGQPDD1148 pKa = 3.69ADD1150 pKa = 3.48GDD1152 pKa = 4.2VAAGAAGSTQQLTVTVRR1169 pKa = 11.84VVQAAEE1175 pKa = 3.97AVGEE1179 pKa = 4.21IANTATVTTIDD1190 pKa = 3.52PALPEE1195 pKa = 4.29VVLTATDD1202 pKa = 3.92DD1203 pKa = 4.43DD1204 pKa = 4.31VDD1206 pKa = 3.88EE1207 pKa = 5.56LIRR1210 pKa = 11.84TAGLTLVKK1218 pKa = 9.56TADD1221 pKa = 3.75PVTVDD1226 pKa = 3.3SVGDD1230 pKa = 3.63VITYY1234 pKa = 10.62SFLATNSGDD1243 pKa = 3.6VTLSAVGVTEE1253 pKa = 4.02TAFTGTGIAPAVTCPSEE1270 pKa = 4.14AATLAPAASVTCTATYY1286 pKa = 9.62TVTQADD1292 pKa = 4.19LDD1294 pKa = 4.13AGIVTNTATAAATAPAGVEE1313 pKa = 4.34GPVSNASTATVTAQALPALTLVKK1336 pKa = 10.45SAGDD1340 pKa = 3.65AVIDD1344 pKa = 3.96SAGDD1348 pKa = 3.47TVSYY1352 pKa = 10.8LFLVTNTGNVTLSDD1366 pKa = 3.64VAIDD1370 pKa = 3.45EE1371 pKa = 4.52TAFTGTGADD1380 pKa = 3.56VVAEE1384 pKa = 4.18CADD1387 pKa = 3.57GVLAPGASVICTASYY1402 pKa = 10.88EE1403 pKa = 4.35VTQPDD1408 pKa = 3.87VNAGRR1413 pKa = 11.84IDD1415 pKa = 3.63NTAVAVATTPTDD1427 pKa = 3.35APVSSEE1433 pKa = 3.91ASSASLVIGAAPALSVVKK1451 pKa = 10.51SATPTDD1457 pKa = 3.66LVVDD1461 pKa = 3.71QEE1463 pKa = 4.42VTYY1466 pKa = 11.16SFVVTNTGNVTLDD1479 pKa = 3.38DD1480 pKa = 3.87VAIDD1484 pKa = 3.6EE1485 pKa = 4.79VEE1487 pKa = 4.29FTGSGALSDD1496 pKa = 4.1VVCAAGAGALDD1507 pKa = 4.88PGDD1510 pKa = 3.9QVICSATYY1518 pKa = 9.44TITQADD1524 pKa = 3.62VDD1526 pKa = 4.83AGTLSNTATATAVAPGGPLTSEE1548 pKa = 4.19PSSVQLPFVQEE1559 pKa = 4.04PGVSVVKK1566 pKa = 10.7SADD1569 pKa = 3.16VDD1571 pKa = 4.23GYY1573 pKa = 11.34AAAGDD1578 pKa = 3.55AVEE1581 pKa = 3.94YY1582 pKa = 10.29RR1583 pKa = 11.84FRR1585 pKa = 11.84VTNTGNVSLADD1596 pKa = 3.66AEE1598 pKa = 4.91VIEE1601 pKa = 4.51QDD1603 pKa = 3.87FSGTGDD1609 pKa = 4.14LSAVDD1614 pKa = 4.3CATGSLLPGQFVDD1627 pKa = 4.12CTADD1631 pKa = 3.66YY1632 pKa = 10.73EE1633 pKa = 4.66VTQADD1638 pKa = 3.43VDD1640 pKa = 3.95AGEE1643 pKa = 4.56LTNSATATGVAPGSADD1659 pKa = 3.83PIEE1662 pKa = 5.03SEE1664 pKa = 4.33LSEE1667 pKa = 4.36LVLPFTGEE1675 pKa = 3.78LALTLTKK1682 pKa = 10.63AGTPVDD1688 pKa = 3.32VDD1690 pKa = 3.27GDD1692 pKa = 3.97GRR1694 pKa = 11.84TTAADD1699 pKa = 4.84RR1700 pKa = 11.84IQWSFTVTNPSAATLSDD1717 pKa = 3.58VTVVDD1722 pKa = 4.44PMAGEE1727 pKa = 4.49IVCDD1731 pKa = 3.82ATVLAPGDD1739 pKa = 4.26SVDD1742 pKa = 3.63CAAVEE1747 pKa = 4.66EE1748 pKa = 4.32YY1749 pKa = 10.78QITAQQAAAGQVLNVASAFAVGVGNVEE1776 pKa = 3.93VAAAEE1781 pKa = 4.05AEE1783 pKa = 4.23AVVEE1787 pKa = 4.47VVTVPPAAAGPGTGGLVSTGVEE1809 pKa = 3.93VRR1811 pKa = 11.84LTLLLGLLALLAGIGLYY1828 pKa = 9.5VAQRR1832 pKa = 11.84RR1833 pKa = 11.84RR1834 pKa = 11.84TRR1836 pKa = 11.84QAA1838 pKa = 2.64

Molecular weight: 185.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2M9CGA0|A0A2M9CGA0_9MICO Uncharacterized protein OS=Diaminobutyricimonas aerilata OX=1162967 GN=CLV46_0419 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3263

0

3263

1073911

29

6075

329.1

35.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.386 ± 0.063

0.436 ± 0.01

6.385 ± 0.043

5.848 ± 0.05

3.123 ± 0.029

8.96 ± 0.042

1.941 ± 0.024

4.214 ± 0.033

1.64 ± 0.031

10.237 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.64 ± 0.021

1.923 ± 0.029

5.568 ± 0.038

2.627 ± 0.024

7.676 ± 0.065

5.379 ± 0.03

6.201 ± 0.083

9.276 ± 0.046

1.516 ± 0.021

2.024 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski