Phaeodactylum tricornutum (strain CCAP 1055/1)

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Ochrophyta; Bacillariophyta; Bacillariophyceae; Bacillariophycidae; Naviculales; Phaeodactylaceae; Phaeodactylum; Phaeodactylum tricornutum

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10465 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B7GBD6|B7GBD6_PHATC Predicted protein OS=Phaeodactylum tricornutum (strain CCAP 1055/1) OX=556484 GN=PHATRDRAFT_49665 PE=4 SV=1
MM1 pKa = 7.69RR2 pKa = 11.84GIFFAASTLVLGIASFRR19 pKa = 11.84ATDD22 pKa = 3.68AQDD25 pKa = 3.46VSCQLVRR32 pKa = 11.84QPNGSVEE39 pKa = 4.26YY40 pKa = 10.65VCDD43 pKa = 3.64QVRR46 pKa = 11.84EE47 pKa = 4.16PMRR50 pKa = 11.84PPLVPDD56 pKa = 3.97GQTYY60 pKa = 8.31QQGPVEE66 pKa = 4.96KK67 pKa = 10.56YY68 pKa = 10.28YY69 pKa = 10.75LQQPAEE75 pKa = 4.46DD76 pKa = 4.04VNASANATIPILRR89 pKa = 11.84RR90 pKa = 11.84VGVPPFDD97 pKa = 4.31TPLQACQGSCGSDD110 pKa = 2.97ADD112 pKa = 4.17CATGLVCLDD121 pKa = 3.27QLDD124 pKa = 4.01RR125 pKa = 11.84PRR127 pKa = 11.84DD128 pKa = 3.65GSVQGCSGNGRR139 pKa = 11.84LTMSVCVVPGSEE151 pKa = 3.92VDD153 pKa = 3.84LKK155 pKa = 10.86IGSLSLEE162 pKa = 4.35KK163 pKa = 11.01CQGTCSSDD171 pKa = 3.42DD172 pKa = 3.57DD173 pKa = 4.38CAGGLACFRR182 pKa = 11.84RR183 pKa = 11.84QGTEE187 pKa = 3.88TVPGCLEE194 pKa = 4.12RR195 pKa = 11.84DD196 pKa = 3.33VSEE199 pKa = 5.91SNFCHH204 pKa = 7.27DD205 pKa = 4.6PNEE208 pKa = 4.27TLARR212 pKa = 11.84LAFVGAAPHH221 pKa = 5.93SQSSLLPTCSGSCSSDD237 pKa = 2.71WDD239 pKa = 3.73CVRR242 pKa = 11.84GSNCFRR248 pKa = 11.84RR249 pKa = 11.84DD250 pKa = 3.12GTEE253 pKa = 3.89SVPGCEE259 pKa = 4.41GRR261 pKa = 11.84GLSGANYY268 pKa = 10.06CYY270 pKa = 10.1IAPEE274 pKa = 4.42GSLLLVGDD282 pKa = 4.43GVTYY286 pKa = 10.19IQYY289 pKa = 10.28PLRR292 pKa = 11.84QCEE295 pKa = 4.11GHH297 pKa = 6.96CIADD301 pKa = 3.66IDD303 pKa = 4.37CEE305 pKa = 4.35SGLKK309 pKa = 10.11CFRR312 pKa = 11.84RR313 pKa = 11.84EE314 pKa = 4.06GSEE317 pKa = 4.73HH318 pKa = 7.04IPGCDD323 pKa = 3.19GEE325 pKa = 4.72GADD328 pKa = 4.13RR329 pKa = 11.84TNYY332 pKa = 9.89CYY334 pKa = 10.36QPFPDD339 pKa = 3.88TSGPTDD345 pKa = 4.05GPTLAPLSVSPSNSPSLILTLDD367 pKa = 3.54PTNSLGSRR375 pKa = 11.84GGEE378 pKa = 3.94SEE380 pKa = 4.59SPSTPTSDD388 pKa = 3.5MPSISPSDD396 pKa = 4.22EE397 pKa = 3.71PSMIPSDD404 pKa = 4.16TPSMVPSDD412 pKa = 4.5VPSMLPSDD420 pKa = 4.74APSMIPSDD428 pKa = 4.36SPSDD432 pKa = 3.75TPSDD436 pKa = 3.8VPSDD440 pKa = 3.99VPSVLPSFSPSMRR453 pKa = 11.84PSDD456 pKa = 4.09TPSTMPSDD464 pKa = 3.86TPTDD468 pKa = 3.81VPSDD472 pKa = 3.94APSDD476 pKa = 4.03SPSDD480 pKa = 3.75VPSDD484 pKa = 3.95VPSDD488 pKa = 3.82APSDD492 pKa = 3.99VPSDD496 pKa = 3.99VPSDD500 pKa = 3.82APSDD504 pKa = 4.09VPSDD508 pKa = 3.81TPSDD512 pKa = 3.91VPSDD516 pKa = 4.05VPSDD520 pKa = 3.81TPSDD524 pKa = 3.91VPSDD528 pKa = 4.05VPSDD532 pKa = 3.71TPSAVPSDD540 pKa = 4.06VPSGNPSDD548 pKa = 4.46VPSEE552 pKa = 4.33TPSDD556 pKa = 4.04VPSDD560 pKa = 3.88TPSDD564 pKa = 3.91VPSDD568 pKa = 3.92VPSDD572 pKa = 3.87VPSDD576 pKa = 3.87VPSDD580 pKa = 3.87VPSDD584 pKa = 3.87VPSDD588 pKa = 3.96VPSDD592 pKa = 3.81TPSDD596 pKa = 3.91VPSDD600 pKa = 4.05VPSDD604 pKa = 3.87TPSDD608 pKa = 3.94LPSDD612 pKa = 4.16VPSDD616 pKa = 3.97VPSTMPSSTLGGTSSNGPVTGKK638 pKa = 10.9GSLAPTGSFSGEE650 pKa = 3.82SSGRR654 pKa = 11.84PSKK657 pKa = 10.09TPGVMVEE664 pKa = 3.9LSARR668 pKa = 11.84PSEE671 pKa = 4.49SFTPSPTTFPVLLPSKK687 pKa = 9.82VPSIAPSRR695 pKa = 11.84TEE697 pKa = 3.74TVAPSTAFPTEE708 pKa = 4.27TSSSLPTLTTTVSSAPTQMCSMSASEE734 pKa = 4.08RR735 pKa = 11.84ASTIMGILEE744 pKa = 3.99EE745 pKa = 4.82DD746 pKa = 4.08FNDD749 pKa = 3.29VSSPQFRR756 pKa = 11.84AVEE759 pKa = 4.02WLVQIDD765 pKa = 4.44PLSLCPGDD773 pKa = 4.27EE774 pKa = 4.27NLEE777 pKa = 3.78QRR779 pKa = 11.84YY780 pKa = 9.1ILAVLYY786 pKa = 8.81FQTGGEE792 pKa = 3.61WWTRR796 pKa = 11.84CSPMSAEE803 pKa = 4.09VCDD806 pKa = 3.56QGEE809 pKa = 4.29AFLSGANEE817 pKa = 4.29CAWGGVNCDD826 pKa = 2.79SSSRR830 pKa = 11.84VTALHH835 pKa = 6.83LDD837 pKa = 3.85SNNLSGSLPSEE848 pKa = 4.62LGRR851 pKa = 11.84LAYY854 pKa = 10.24LVEE857 pKa = 5.8LDD859 pKa = 3.84MDD861 pKa = 4.99DD862 pKa = 4.97NEE864 pKa = 4.68LTGSIPRR871 pKa = 11.84ILGQLSFLEE880 pKa = 5.02IVDD883 pKa = 5.28LDD885 pKa = 4.26DD886 pKa = 4.08NQLTGSIPEE895 pKa = 4.0EE896 pKa = 4.32LYY898 pKa = 10.85SVSSLEE904 pKa = 5.28ILDD907 pKa = 5.23LDD909 pKa = 3.96INQLTGTISTLIGNLVNLYY928 pKa = 10.01YY929 pKa = 10.86LQIDD933 pKa = 3.92SNKK936 pKa = 8.07FTGSIPSEE944 pKa = 4.14VGTLTRR950 pKa = 11.84LEE952 pKa = 4.39YY953 pKa = 10.77FSMTDD958 pKa = 3.07IQIAEE963 pKa = 4.51ALPDD967 pKa = 3.91SLCSRR972 pKa = 11.84DD973 pKa = 3.58TLLLFGDD980 pKa = 4.5CEE982 pKa = 4.4VCVVEE987 pKa = 5.33DD988 pKa = 4.06CCTACLAKK996 pKa = 9.73EE997 pKa = 4.25TTPP1000 pKa = 4.07

Molecular weight: 104.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B5Y3A4|B5Y3A4_PHATC Predicted protein (Fragment) OS=Phaeodactylum tricornutum (strain CCAP 1055/1) OX=556484 GN=PHATR_13439 PE=4 SV=1
MM1 pKa = 7.5TKK3 pKa = 9.03RR4 pKa = 11.84TLSNKK9 pKa = 9.3SRR11 pKa = 11.84YY12 pKa = 9.39SVLKK16 pKa = 10.53LSGFRR21 pKa = 11.84SRR23 pKa = 11.84MATPQGRR30 pKa = 11.84KK31 pKa = 7.39TIRR34 pKa = 11.84NRR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.85KK39 pKa = 10.07GRR41 pKa = 11.84KK42 pKa = 8.84NLTLRR47 pKa = 11.84RR48 pKa = 3.92

Molecular weight: 5.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10451

14

10465

4838762

29

4825

462.4

51.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.512 ± 0.02

1.569 ± 0.01

5.893 ± 0.016

6.083 ± 0.019

3.747 ± 0.013

6.394 ± 0.021

2.462 ± 0.011

4.53 ± 0.015

4.659 ± 0.018

9.452 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.253 ± 0.009

3.785 ± 0.012

5.188 ± 0.022

4.091 ± 0.014

6.148 ± 0.017

8.372 ± 0.026

6.125 ± 0.015

6.834 ± 0.017

1.347 ± 0.009

2.555 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski