Maize necrotic streak virus (MNeSV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Zeavirus

Average proteome isoelectric point is 8.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9E349|Q9E349_MNESV p21 protein OS=Maize necrotic streak virus OX=137556 PE=3 SV=2
MM1 pKa = 7.32EE2 pKa = 5.14RR3 pKa = 11.84AIQGSDD9 pKa = 2.18AWQQTGGQRR18 pKa = 11.84RR19 pKa = 11.84VGGCGDD25 pKa = 3.54SFAPFQLPDD34 pKa = 3.58EE35 pKa = 5.1SPTSDD40 pKa = 3.03EE41 pKa = 3.91WRR43 pKa = 11.84LHH45 pKa = 5.95HH46 pKa = 7.37DD47 pKa = 4.27AYY49 pKa = 10.97DD50 pKa = 4.66PDD52 pKa = 4.22TDD54 pKa = 4.0CPLGFKK60 pKa = 9.65EE61 pKa = 4.42FWSVGKK67 pKa = 10.36AISKK71 pKa = 9.95RR72 pKa = 11.84YY73 pKa = 9.01HH74 pKa = 6.01RR75 pKa = 11.84YY76 pKa = 8.7DD77 pKa = 3.0WKK79 pKa = 10.86EE80 pKa = 3.53ASLDD84 pKa = 3.53RR85 pKa = 11.84ALGSWQGDD93 pKa = 3.67KK94 pKa = 11.14VITEE98 pKa = 4.35ASRR101 pKa = 11.84FLGVDD106 pKa = 3.33QVSCTYY112 pKa = 10.1SIRR115 pKa = 11.84VRR117 pKa = 11.84GVSITLSGGSRR128 pKa = 11.84ALLRR132 pKa = 11.84LVSMADD138 pKa = 3.45RR139 pKa = 11.84IKK141 pKa = 10.75RR142 pKa = 11.84SEE144 pKa = 4.0LQFATSAVEE153 pKa = 4.2SVVSRR158 pKa = 11.84GCPEE162 pKa = 3.7EE163 pKa = 4.03EE164 pKa = 4.09TPKK167 pKa = 10.66EE168 pKa = 4.22SEE170 pKa = 3.94

Molecular weight: 18.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9E350|Q9E350_MNESV Capsid protein OS=Maize necrotic streak virus OX=137556 PE=3 SV=1
MM1 pKa = 7.64DD2 pKa = 5.26AEE4 pKa = 4.55YY5 pKa = 10.51EE6 pKa = 4.35QVSRR10 pKa = 11.84PWNEE14 pKa = 3.79LYY16 pKa = 10.69KK17 pKa = 10.71EE18 pKa = 4.0ATLGNKK24 pKa = 9.61LVVNVGSEE32 pKa = 4.03DD33 pKa = 3.76AEE35 pKa = 4.41IPLLPSNYY43 pKa = 8.3LTKK46 pKa = 10.78ARR48 pKa = 11.84LAMSGGYY55 pKa = 7.91ITMRR59 pKa = 11.84MIRR62 pKa = 11.84IRR64 pKa = 11.84IVPLVSRR71 pKa = 11.84NSGVSGRR78 pKa = 11.84LFLRR82 pKa = 11.84DD83 pKa = 3.2ITDD86 pKa = 3.41TTGKK90 pKa = 9.99KK91 pKa = 9.61LHH93 pKa = 5.68STEE96 pKa = 4.74LLDD99 pKa = 4.09LGKK102 pKa = 10.39EE103 pKa = 3.62IRR105 pKa = 11.84LSLRR109 pKa = 11.84HH110 pKa = 6.35LDD112 pKa = 3.79FSVSTRR118 pKa = 11.84SAVPIVFGFEE128 pKa = 4.1EE129 pKa = 4.69LVSPYY134 pKa = 10.95LEE136 pKa = 4.09GRR138 pKa = 11.84EE139 pKa = 3.97LFSVSFRR146 pKa = 11.84WQIGLSAQSYY156 pKa = 10.02SLPQVPWKK164 pKa = 10.71VLYY167 pKa = 10.68QEE169 pKa = 4.34DD170 pKa = 3.7ALRR173 pKa = 11.84RR174 pKa = 11.84KK175 pKa = 9.63LPKK178 pKa = 9.83KK179 pKa = 10.18ANKK182 pKa = 8.65TNSPPNVV189 pKa = 3.33

Molecular weight: 21.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1671

170

788

334.2

37.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.84 ± 0.832

1.795 ± 0.478

4.907 ± 0.482

5.745 ± 0.599

3.172 ± 0.507

7.66 ± 0.467

1.137 ± 0.4

4.967 ± 0.376

5.925 ± 0.339

8.677 ± 1.007

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.753 ± 0.377

3.232 ± 0.457

4.428 ± 0.346

2.932 ± 0.316

7.54 ± 0.544

6.882 ± 1.064

5.625 ± 0.794

9.096 ± 0.593

1.735 ± 0.175

3.89 ± 0.259
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski